Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,283,465	G→A	P480S (CCG→TCG)	actP ←	acetate/glycolate:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,283,465	0	G	A	100.0%	210.7 / NA	68	P480S (CCG→TCG)	actP	acetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/30);  total (38/30)

ATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACG  >  NC_000913/4283219‑4283708                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aTTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGcc                                                                                                                                                                                                                                                  <  2:303331/250‑1 (MQ=255)       cGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGatc                                                                                                                                                                                                                                           <  2:137044/251‑1 (MQ=255)            aGACGACCGGGGCAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGatcatc                                                                                                                                                                                                                                        >  1:505382/1‑249 (MQ=255)             gacgacCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGt                                                                                                                                                                                                                                     >  2:525481/1‑251 (MQ=255)              acgacCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCACCGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGta                                                                                                                                                                                                                                    <  2:509601/251‑1 (MQ=255)                  ccGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGTAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAAGCGTCGAGCCGATGATCATCAGtactac                                                                                                                                                                                                                                <  1:133390/251‑1 (MQ=255)                  ccGGGGAAGGGGAGAGATGAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCCCGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGtactac                                                                                                                                                                                                                                <  2:18671/251‑1 (MQ=255)                            ggAGAGATTAGTGCGCGCGGCCTTGCTCACCGCCAAAGCCGGTGTGGGAGCGGATCAACTGCGCGCGGAACAGTTCACGATCACGCGCGCCTTCCGCTGCGTTCTCGGTTGCCGAGAAGAACCAGATGCCGAGGCATGCCACGGTGATAGAGCACAACGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATc                                                                                                                                                                                                                      >  2:550409/1‑251 (MQ=255)                             gagagaTTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCa                                                                                                                                                                                                                     <  1:339483/251‑1 (MQ=255)                                          gcgcgGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCagccagcc                                                                                                                                                                                                        >  1:386751/1‑251 (MQ=255)                                            gcgtcctagCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTGCCCGCTCACGCGCGCCTTCCGCTGAGTGATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCTTCGGTGATAGAGAACAGCCCCGGGTATTCATCCGGGAAGATGGCTTTTTCGTGTCCAAGGATCTGTAACCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCAc                                                                                                                                                                                                      <  1:46870/243‑1 (MQ=255)                                                                aGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGCTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGGATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCACCATCGCGCCAAGCGTg                                                                                                                                                                                  >  2:401888/1‑251 (MQ=255)                                                                   cGGTCTGGGAGCCGATCAACAGCGAGAGGAACCGCTCACGCTCACGCGCGCCTTTCGCAGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCAACGGTGATAGTGAACAGCGCCGAGAATTCTTACGGCAAGACGGCTTATTAGGGAACACGAATCTGTAACCACATCGTCGAGCAGAGGATCGTCAGTACCACTGCGGGAATCCACCCCAGCCACACACCCTTCACCGCGCCACGCGTgtt                                                                                                                                                                                >  2:396050/1‑248 (MQ=255)                                                                    ggTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTc                                                                                                                                                                               >  1:386430/1‑250 (MQ=255)                                                                        tGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGttt                                                                                                                                                                          >  1:505551/1‑251 (MQ=255)                                                                          ggAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGCGGTCAGTTTCg                                                                                                                                                                        >  2:384242/1‑251 (MQ=255)                                                                                  tAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGCGATAGCGAACAGCGCCGGGTATTCACACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCACCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTAc                                                                                                                                                                >  1:593205/1‑251 (MQ=255)                                                                                  aaaaCTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTAc                                                                                                                                                                <  2:563917/250‑1 (MQ=255)                                                                                     acggcgcgcGGAACAGTTCACGCTCACGCGCGCCTTCCTCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCGGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGGCAGTTTCGACCAGTACATa                                                                                                                                                             <  2:386751/248‑1 (MQ=255)                                                                                         cgcgcgGAACAGTTCACGATCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGCGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAACCAGCCCCGGCCAGCAACCCATAATCGCGCAAGGCGGGGCCGGTTTGGACCAGAACATaaaa                                                                                                                                                          >  1:546067/1‑247 (MQ=255)                                                                                              ggAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGc                                                                                                                                                       >  1:445609/1‑248 (MQ=255)                                                                                              ggAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGCGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGc                                                                                                                                                       >  1:388916/1‑248 (MQ=255)                                                                                                    gtgcacgctcacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATc                                                                                                                                              <  2:3424/248‑1 (MQ=255)                                                                                                                  gcgcCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCt                                                                                                                                >  1:562796/1‑251 (MQ=255)                                                                                                                    gcCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTcg                                                                                                                              >  2:152089/1‑251 (MQ=255)                                                                                                                          cGCTGAGTTATCGGTTGCCGAGCAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAGCAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCcgcg                                                                                                                         >  2:107593/1‑250 (MQ=255)                                                                                                                                     cGGTTGCCGAGAAGTACCAGATGCCGAGGAATGCCACGTTGTTAGAGAACAGTGCCGGGTATTAATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTCCTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGAGCGTGAAGTTACAGCTCGCCGCGATGGCAAACGcc                                                                                                             <  1:462889/251‑1 (MQ=255)                                                                                                                                       gTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGTGTATTCATACGGGAAGATGTCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAg                                                                                                           <  1:148572/251‑1 (MQ=255)                                                                                                                                             gagaAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCccac                                                                                                     >  2:164724/1‑251 (MQ=255)                                                                                                                                                 agaaCCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCAt                                                                                                  >  2:201870/1‑250 (MQ=255)                                                                                                                                                      cAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGc                                                                                            <  1:341553/251‑1 (MQ=255)                                                                                                                                                       aGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCg                                                                                           <  1:134350/251‑1 (MQ=255)                                                                                                                                                            ccGAGGAATGCCACGGTAATAGAGAACAGCGACGTGTATTCAGACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGtt                                                                                      <  2:100757/251‑1 (MQ=255)                                                                                                                                                              gAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCt                                                                                    >  1:278946/1‑251 (MQ=255)                                                                                                                                                              gAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCt                                                                                    <  1:460881/251‑1 (MQ=255)                                                                                                                                                                   aTGCCCCGGTGATAGTGAACAACGCCGGGTATTAATACGGGAAGATGGCTTTTTCATGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAACCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGCATGATCGGGACGTTACAGACCGCCGCGATGGCCAACGCAACCCCCACCATAAAGGCGTTGTTCTGATtc                                                                               >  2:134759/1‑251 (MQ=255)                                                                                                                                                                    ggCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATtct                                                                              <  1:60660/250‑1 (MQ=255)                                                                                                                                                                       cACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTTCTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCaa                                                                           <  1:434980/251‑1 (MQ=255)                                                                                                                                                                          ggagATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAAc                                                                         >  2:175036/4‑250 (MQ=255)                                                                                                                                                                           gTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGAGCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAg                                                                       <  1:281398/251‑1 (MQ=255)                                                                                                                                                                             gATAGAGAACAGGGCCGGGTATTCATACGGGAAGATGGCTGTTTCGTGACCAAGGATCTGTACCCAAACCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGc                                                                      <  2:536244/250‑1 (MQ=255)                                                                                                                                                                                 gagaACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGAGGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACgac                                                                 <  2:505551/251‑3 (MQ=255)                                                                                                                                                                                    aaCAGCGCCGGGTATTCAGACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAg                                                              <  2:483936/251‑1 (MQ=255)                                                                                                                                                                                     aCAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTGCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGAGGGCAAACGCCAGCCCCGCCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAgg                                                             <  2:562796/251‑1 (MQ=255)                                                                                                                                                                                                tATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAAt                                                  >  1:31694/1‑251 (MQ=255)                                                                                                                                                                                                    catgcGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAACCGGCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGGTACAGCTCGCCGCGATGGCAAACGCCAGCCCCTCCATAAAGGCGATGTTCTGGTTCTCGAACATCACGCCGAGGATAATCGCGATCACg                                              <  2:593205/247‑1 (MQ=255)                                                                                                                                                                                                         gggAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCg                                           >  1:479413/1‑249 (MQ=255)                                                                                                                                                                                                           gAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCCGTTTCGACCAGAACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAgg                                        >  1:251865/1‑250 (MQ=255)                                                                                                                                                                                                            aaGATGGCTTTGTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGAt                                      <  1:525481/251‑1 (MQ=255)                                                                                                                                                                                                                 ggCTTTTTCGTGAACACGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCAAGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACCGCACGCCGAGGATAATCGCACTCACGCCGCGGATCAgc                                  >  2:360160/1‑249 (MQ=255)                                                                                                                                                                                                                      tttCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGACTGCTCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTg                            >  1:95870/1‑251 (MQ=255)                                                                                                                                                                                                                       ttCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGTTGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTg                            >  1:23413/1‑250 (MQ=255)                                                                                                                                                                                                                          gTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTCCGGTGAttt                        >  2:189493/1‑251 (MQ=255)                                                                                                                                                                                                                             aCCAAGGATCTGTACCCAAATCGGCGAGCCGAGGATCATCAGGACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTGCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTg                     <  2:31694/251‑1 (MQ=255)                                                                                                                                                                                                                             aCCAAGGATCTGTACCCAAAACGTCGAGCCTAGGATCACCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCGGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTg                     <  2:58446/251‑1 (MQ=255)                                                                                                                                                                                                                              ccAAGGATCTGTAACCAAATCGTCAAGCCGCGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACCTAGAAAGCCGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGCTGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTg                     >  2:430089/1‑250 (MQ=255)                                                                                                                                                                                                                               cAAGGATCTGTGCCCAAAGCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGAt                   <  2:505382/251‑1 (MQ=255)                                                                                                                                                                                                                                   gATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCCGTTTCGACCAGCACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGACGAGGATCAGTACGGTGATTTTTGATcccc               >  1:613653/1‑251 (MQ=255)                                                                                                                                                                                                                                      cTGTACCCAAATCGTCGAGCCGAGGATCATCATTACTACTGCGGTACTCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAAAGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCCAACAGCACGCCGAGGATAATAGCAATCACGCCGAGGATCGGCACGGGGATTTTTGATACCCGc             >  2:156177/1‑250 (MQ=255)                                                                                                                                                                                                                                      cTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGc             >  1:209966/1‑250 (MQ=255)                                                                                                                                                                                                                                        gTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATAccc               <  1:215689/246‑1 (MQ=255)                                                                                                                                                                                                                                         tACCCAAATCGTCGAGCCGAGGATCATCAGAACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACACCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGcc         >  1:79519/1‑250 (MQ=255)                                                                                                                                                                                                                                          aCCCAAATCGTCGAGCCGCGGATCATCAGTACTACTGCGGTCATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGctc        >  1:200034/1‑251 (MQ=255)                                                                                                                                                                                                                                          aCCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCCAGGATCAGTACGGTGATTTTTGATACCCGCAGctc        >  2:169107/1‑251 (MQ=255)                                                                                                                                                                                                                                            ccAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGAGCAGTACGGTGATTTGTGATACCCGCAGCTCtt      <  1:189493/251‑1 (MQ=255)                                                                                                                                                                                                                                             cAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCAACGCGTGGTCAGTTTAGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATAACGCCGAGGATCAGGACGGAGATTTTTGATACCCGCAGCTCTTc     >  1:288849/1‑251 (MQ=255)                                                                                                                                                                                                                                              aaaTCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTc     >  2:367982/1‑250 (MQ=255)                                                                                                                                                                                                                                                aTCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTAAGGTGATTTTTGATACCCGCAGCTCTTCACg  >  2:454316/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACG  >  NC_000913/4283219‑4283708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑