Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,275,204 C→T intergenic (‑163/‑267) yjcB ← / → pdeC uncharacterized protein YjcB/c‑di‑GMP phosphodiesterase PdeC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,275,2040CT98.0% 157.7 / NA 52intergenic (‑163/‑267)yjcB/pdeCuncharacterized protein YjcB/c‑di‑GMP phosphodiesterase PdeC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/31);  minor base G (0/1);  total (20/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCG  >  NC_000913/4274958‑4275442
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
gTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTGTGTTAGTCat                                                                                                                                                                                                                                             <  2:238968/250‑1 (MQ=255)
  tGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCatat                                                                                                                                                                                                                                           <  1:58772/250‑1 (MQ=255)
    aGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAATTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAg                                                                                                                                                                                                                                        <  1:338624/251‑1 (MQ=255)
       gtgCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGcc                                                                                                                                                                                                                                     <  1:340630/251‑1 (MQ=255)
       gtgCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGGTTGTTAGTCATATTAGGc                                                                                                                                                                                                                                      <  2:448322/250‑1 (MQ=255)
          ggggacAGAAACATCATTACGGTACGAAGAATATGCCAGAGAAGAAGAACAACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGGCGATGACCTGGACAGCGCGGAGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGGGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAATTATATCAAAATGGATTGTTTGTTAGTCATATTAGGcattg                                                                                                                                                                                                                                  <  2:55000/246‑5 (MQ=255)
            ggcaaGAAACATCATTACGGAACTAAGAAGGTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACGCTGTGTGTTTCATTAATTTTGGGAACTAGATCACAATTGATTGGTTGTTAGTCATATTAGGCCGTg                                                                                                                                                                                                                                  <  2:277607/245‑1 (MQ=255)
                aGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACtttt                                                                                                                                                                                                                            <  2:458017/251‑1 (MQ=255)
                aGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACtttt                                                                                                                                                                                                                            <  1:510302/251‑1 (MQ=255)
                         atTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTa                                                                                                                                                                                                                   <  1:143102/251‑1 (MQ=255)
                            aCGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGCGCGGGCAGCCATCAAACAATTCCTCTGAACAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTAATATTAGGCCGTGACTTTTATTGCTGTAc                                                                                                                                                                                                                  >  1:579205/1‑249 (MQ=255)
                              ggCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGGGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACATCGCGGCGGTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCTCAATTGAGTGTTTGTTAGTTATATTAGGCCGTGACTTTTATTGTTGTTCAGAt                                                                                                                                                                                                              <  2:613380/251‑1 (MQ=255)
                                         gTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGtttt                                                                                                                                                                                                   >  1:501993/1‑251 (MQ=255)
                                           tGCCAGCGAAAAAGAACCACGCCAGTGGGGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGGTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTGAGTCATATTAGGCCGTGACTTTCATTGCTGTACAGATTATGTGGttttt                                                                                                                                                                                                  <  2:274530/250‑1 (MQ=255)
                                             ccAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCTTCTGAAAAGAGCCGAAGCACTGGACAGAGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAACATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACACTTGATTGTTTGTTAGTCATATTAGGACGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTc                                                                                                                                                                                                 >  1:374391/1‑249 (MQ=255)
                                                  gaagaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGc                                                                                                                                                                                           >  2:622059/1‑250 (MQ=255)
                                                    agaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAtt                                                                                                                                                                                        <  1:519237/251‑1 (MQ=255)
                                                         aaCCACGCCAGTGGTGAGGGTATCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAggg                                                                                                                                                                                   >  1:492639/1‑251 (MQ=255)
                                                                ccAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCt                                                                                                                                                                            >  1:511626/1‑251 (MQ=255)
                                                                     gggtaGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGTACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGTGCTTGCAGAACACAAAAATGAAACACTCTGTGTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTTCAGATTATGTGGTTTTTCAGTGGCATTAATGCCATACCTTCccg                                                                                                                                                                       <  2:383110/247‑1 (MQ=255)
                                                                          gggTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCgccgcc                                                                                                                                                                  >  1:74920/1‑251 (MQ=255)
                                                                               gCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGc                                                                                                                                                             <  1:363032/251‑1 (MQ=255)
                                                                                     aaaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCt                                                                                                                                                       <  1:261437/251‑1 (MQ=255)
                                                                                         aaTTCCTCTGAAAAGAGCCGATGCCCTGGCCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGt                                                                                                                                                     >  1:445049/1‑249 (MQ=255)
                                                                                                ctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaat                                                                                                                                              <  1:329877/249‑1 (MQ=255)
                                                                                                     aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGGGGTTTTTCAGTGGCATTAAGGGCTTATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAAtttt                                                                                                                                       <  2:294557/251‑1 (MQ=255)
                                                                                                                    ggACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGACACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAGTGTTTGTTTGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTCGGAAATTAATTTTGAATATCAATGAatt                                                                                                                        >  1:81480/1‑251 (MQ=255)
                                                                                                                    ggACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAatt                                                                                                                        <  2:257269/251‑1 (MQ=255)
                                                                                                                        aGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTAttt                                                                                                                    >  1:446039/1‑251 (MQ=255)
                                                                                                                        aGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTAttt                                                                                                                    <  2:138502/251‑1 (MQ=255)
                                                                                                                            cggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaattaat                                                                                                                                          >  2:510078/1‑225 (MQ=255)
                                                                                                                            cggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaattaat                                                                                                                                          <  1:510078/225‑1 (MQ=255)
                                                                                                                            aggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCAt                                                                                                                >  2:21748/2‑251 (MQ=255)
                                                                                                                                          ggAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTa                                                                                                  <  1:354924/251‑1 (MQ=255)
                                                                                                                                             ggCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGaa                                                                                               <  1:194391/251‑1 (MQ=255)
                                                                                                                                                                      gggCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCaa                                                                       >  1:352851/1‑250 (MQ=255)
                                                                                                                                                                       ggCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGACAGGTATCAATTTCAAATCAGGCaaa                                                                      >  1:123796/1‑250 (MQ=255)
                                                                                                                                                                            gCAGAACACAAAAATGAAACACTCTTTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGGCATATTAGGCCGTGATTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATGTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAAGCAGGCAAAAGTGCt                                                                <  2:445049/251‑1 (MQ=255)
                                                                                                                                                                                   acaAAAATGTAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATGGATTGTTTGTTATTCATATTAGGCCGTGGCTTTTATTGCTGTACAGATTATGTGGGTTTTCAGTGTCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTtata                                                         <  1:532480/251‑1 (MQ=255)
                                                                                                                                                                                      aaaaTGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCg                                                      <  2:501993/251‑1 (MQ=255)
                                                                                                                                                                                          tGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGCTTGCTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTACGGGCATATATTCACGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCACTTTCAAATCAGGCAAACGTGCTATTTATACCGTa                                                    >  1:549360/1‑249 (MQ=255)
                                                                                                                                                                                              acacTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGAttt                                              <  1:84909/251‑1 (MQ=255)
                                                                                                                                                                                                  tctGTATGATTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGACATATTAGGCCGTGACTTTTATTGATGTACAGAGTATGTGGTTTTTCAGTGGCATTAATGGCATATCTTCACGCCGCCTCTGCCTTCCTGTAGGACATTAATTTTGAGTATCTATGAATTATTTTCATCCAGGTGACGCTTAGAAAGGTATCAATTTCAAATTATGCAACAGTGCTCTTTATACCGTAAGATTTATCt                                          >  2:448006/1‑251 (MQ=255)
                                                                                                                                                                                                            tCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTc                                 <  1:329336/250‑1 (MQ=255)
                                                                                                                                                                                                            tCATTAATTTTGTGAAATATATCACAATTGATTGTTTGTTAGTTAGATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTTGTTTTTCAGTGGCATTAAGGACATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAAGCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGGCGtt                                 <  2:81480/250‑2 (MQ=255)
                                                                                                                                                                                                                 aaTTTTGTGAACTATATCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTAcc                           <  2:72681/251‑1 (MQ=255)
                                                                                                                                                                                                                                atCACAATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTGATCTAAAGACGTCGGTACCCAGGGTTTTCACCtt            <  2:446039/251‑1 (MQ=255)
                                                                                                                                                                                                                                  cacaATTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGc          >  1:21218/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aaTTGATTGTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAAt       <  1:339986/251‑1 (MQ=255)
                                                                                                                                                                                                                                       ttgattgTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATgg     >  2:593912/1‑251 (MQ=255)
                                                                                                                                                                                                                                       ttgattgTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATgg     >  2:178237/1‑251 (MQ=255)
                                                                                                                                                                                                                                          attgTTTGTTAGTCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCg  >  1:345667/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
GTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCG  >  NC_000913/4274958‑4275442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑