BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,193,996	T→G	D70A (GAT→GCT)	thiE ←	thiamine phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,193,996	0	T	G	100.0%	353.1 / NA	116	D70A (GAT→GCT)	thiE	thiamine phosphate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (47/69); total (47/69)

Only 100 of 116 total aligned reads displayed.

GCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCT  >  NC_000913/4193751‑4194242

gCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTg                                                                                                                                                                                                                                                     >  1:384405/1‑251 (MQ=255)

 ccGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAACTCTTCCGGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGGAAGCGTTg                                                                                                                                                                                                                                                     >  2:172018/1‑250 (MQ=255)

   gTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATa                                                                                                                                                                                                                                                  <  1:16705/251‑1 (MQ=255)

     gccgtcggTTCCGGCCCGTGCGGTGCAGAGGTCATCGGTTTGGTGTGCGTCGGCAACCCAGGTCGCAGCGCGATATAAGAGGGGCGTGCTGCCCGCGCGACGTCGAGTGCCATATCGTCAGGGGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCTGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCTTTGATaa                                                                                                                                                                                                                                                 <  2:176946/243‑1 (MQ=255)

      ccAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAAc                                                                                                                                                                                                                                               >  2:147645/1‑251 (MQ=255)

        aGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACa                                                                                                                                                                                                                                              >  1:178938/1‑250 (MQ=255)

              tCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATcgcg                                                                                                                                                                                                                                        <  1:22484/250‑1 (MQ=255)

               ccAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATcgcgcg                                                                                                                                                                                                                                      <  1:551210/251‑1 (MQ=255)

                cAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTCGGGTCGGGAACACATGTCCCAGCGAGCTTTAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCAATATCGTCATGTGGCGAAAGGCCCAG‑CCGCAGGCCTGCCGCGCGGAGGGCATGGAGATCGGTGGCTTGCAAATCTCCCTGCCCCAAATGGACGCCATACGCCTGATGCTTTATCGCCAGCAGCCAGTAAGCGTTGATAAACAATCGCGCGt                                                                                                                                                                                                                                     <  2:428017/251‑1 (MQ=255)

                 cgCCCCTGGGGTGCAGAGGGCATCTGTTTGGTTTGCGGCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGtt                                                                                                                                                                                                                                    <  2:397591/250‑1 (MQ=255)

                   cccgcgcggttcgtaggggagctttttggttagCGTCGGGAACACTTGGCCAGGCGAGAATTAAGAGGGGCGTGCGTCCAGCGCGTCGCCGTTGTCCATATCGTCATGTGTAGAAACGCCCAG‑CCGCAGGCCGGCCGCGCGGATGGCAGTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGTTGCTGGAACGCCAGCCGCCATTAAGCGTTGATAAACAATCGCGCGTta                                                                                                                                                                                                                                   <  2:463230/226‑1 (MQ=255)

                     ccTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTtat                                                                                                                                                                                                                                  >  1:438189/1‑249 (MQ=255)

                     ccTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCCCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCAAG‑CCGCAGGACTGCCGCGCGGCTGGCATTGAGATCGGAGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCCTACGCCTGCTGCTTGATCGCCAGCCGCCAGTACGCGTTGATAAACAATCGCGCGtcat                                                                                                                                                                                                                                  >  1:398065/1‑246 (MQ=255)

                        gCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAgc                                                                                                                                                                                                                               <  1:298227/249‑1 (MQ=255)

                             gCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATAGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAgcggcgtcc                                                                                                                                                                                                                        >  1:523761/1‑248 (MQ=255)

                              cAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGGTATCAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGAGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGccc                                                                                                                                                                                                                       >  1:416926/1‑251 (MQ=255)

                              aaGAAGGCATCTGTTTGGTTTGCGGCGGGAACACATGTCCCAGGGCGATAGAAGAGGGGCGGGCTGCCAGCGCGGCGTCGATTTCCATATCGTCATTTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAACCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCTGCGGccc                                                                                                                                                                                                                       <  1:515508/250‑1 (MQ=255)

                                  aggcttcggtttggtttgCGGCGGGACCACAGGCCCCTGCGCGGAATAACAGGGGCGTGCTGCCAGCGCGAGGTCGATTCCCATATCGTCATGTGTTGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGACCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGTCCCAgcg                                                                                                                                                                                                                   <  2:50603/243‑1 (MQ=255)

                                  aGGCATCTGTTTGGTTTGCGTCGGGAACACATGGCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAgcg                                                                                                                                                                                                                   <  2:500993/251‑1 (MQ=255)

                                  aGGCATCTGTTTGGTTGGGGTGGGGAACACATGCCCCAGCTGGATATAAGAGGGGCGTGCGGCCAGCGCGACGTCAATTTCCATAGCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGAGGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACTCCATACGCCTTGTGGTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGTCCAgcg                                                                                                                                                                                                                   <  2:592486/251‑1 (MQ=255)

                                          gtttgtttggCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTgc                                                                                                                                                                                                            <  1:33800/250‑1 (MQ=255)

                                          gtttggtttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCCGCGCAATTgcc                                                                                                                                                                                                           >  1:508840/1‑251 (MQ=255)

                                          gtttggtttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAAt                                                                                                                                                                                                               <  1:147645/247‑1 (MQ=255)

                                              ggttggCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCTCGACGTCGATTTCCATATCGTCATGTTCCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCGTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCa                                                                                                                                                                                                       <  2:508840/251‑1 (MQ=255)

                                                  ggggTCGGGACCACTGGTCCCAGCGCGATAAAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATACCGTCATGTGTCGAACCGCCCAG‑CCGCAGGCCGGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGAGGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGAc                                                                                                                                                                                                   <  2:86037/248‑1 (MQ=255)

                                                  ggCGTGGGGAACACAGGTCCGAGCGCGATAAAGGAGGGGGGGGCTGCCAGTGGGACGTGGATTCCAATACCGCCGGGGGTCGAACCGCCCAG‑CCGCAGGCTGGCGGCGCGGAGGGCATTCAGAGCGGTGGCTTGCAAATCGTCCTGCCCCAAAGGGAGCCCATGCGCCTGATGCGTGATCGCCAGCCGCCAGTAAGCGGTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATGGCCGCCACGAc                                                                                                                                                                                                   <  2:208987/250‑1 (MQ=255)

                                                             cacaTGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTcc                                                                                                                                                                                         >  1:299271/1‑250 (MQ=255)

                                                               caTGCCCCAGCGCGATATAAGAGGGGCGTGCTGCCGGCGCCACGTCGATTCCCATATCGTCATGTGGCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGAGCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCAcc                                                                                                                                                                                      <  2:215517/251‑1 (MQ=255)

                                                                  ggcccAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACctct                                                                                                                                                                                   <  1:500813/249‑1 (MQ=255)

                                                                    cccAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCGGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTc                                                                                                                                                                                 >  2:171259/1‑251 (MQ=255)

                                                                      cAGCGCGATATAAGAGGGGGGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCGTGCAAAGCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCAt                                                                                                                                                                               <  1:535876/251‑1 (MQ=255)

                                                                          gcgATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATcgcg                                                                                                                                                                           <  1:469041/251‑1 (MQ=255)

                                                                               ataAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGAt                                                                                                                                                                      >  1:67365/1‑251 (MQ=255)

                                                                                 aaGAGGGTCGTTCGGCCAGCGCGTCGTCGATTTCCATATCGTCATTTGGCAAAAGGCCCAG‑CCGCAGGCCTGCCGGTCGGATGGCATTGAGATCGGTGGCTTTCAAATCTTCCTGCCCCAAATGGACGCCATACCCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAAGCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCt                                                                                                                                                                    <  1:598633/251‑1 (MQ=255)

                                                                                    agGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTg                                                                                                                                                                 >  1:401559/1‑251 (MQ=255)

                                                                                          gTGCTGCCAGCGCGACGTCGATTCCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGt                                                                                                                                                           <  1:350002/251‑1 (MQ=255)

                                                                                           tgctgcCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGCTCTTTGATGCGt                                                                                                                                                           >  1:288959/1‑250 (MQ=255)

                                                                                            gcgcccAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATGGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAg                                                                                                                                                         <  2:611014/247‑1 (MQ=255)

                                                                                                   gcgggACGTCGATTCCCCTACCGTCAGGTATCGAAACGCCAAG‑CCGAAGGCCTGCGGCGCGGATGGCATTGAGACGGGTGGCTTGCAATGCTCCCTGCCCCAAAGGGCGGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCTTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGgaga                                                                                                                                                  <  2:146995/247‑1 (MQ=255)

                                                                                                    cgcgACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCCCCCCAATTTCCGCCCCGACATCGGCTTCCACCTCTTCATCGCGGCGCTCTTTGCTGCGTAGCTGgag                                                                                                                                                   >  2:585624/1‑249 (MQ=255)

                                                                                                       gACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGAGGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtac                                                                                                                                              <  1:11954/251‑1 (MQ=255)

                                                                                                         cGTCGATTTCCATATCGTCATTTGCCGAAAGGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtacgt                                                                                                                                            <  1:141591/251‑1 (MQ=255)

                                                                                                              attccaattgtgttaTGTGGCGAACCGCCCAC‑CCGCGGGCCTGCCCCGCGGATGGCATTGAGACCGGTGGCTTGTAAATCTCCCTGCCCCAAATGGACTCCATCCGCCTGATGCTTGATCGCCGCCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATGGCCGCCACGACATCGGCTTCCTCCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGcc                                                                                                                                       <  2:222606/237‑1 (MQ=255)

                                                                                                                  ccATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGc                                                                                                                                    >  1:76518/1‑250 (MQ=255)

                                                                                                                     tatCGTCATGTGTCGAACCGCAAAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATAGCGCCGATCTTTGATGCGTAGCTGGAGAGCACGTACGCCTGCATcc                                                                                                                                >  2:430846/1‑251 (MQ=255)

                                                                                                                     tatCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATCGCCTTGCGATCGGTGGCTTGCAAATCTTCCAGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCAAGCCGCCAGTAAGCGTTGATAAACACTCGCGCGTTATAGCGGCGGCCCAGCGGAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGCTATTTGATGCGTAGCTGGAGAGTACGTACGCCTTCATcc                                                                                                                                >  1:93051/1‑251 (MQ=255)

                                                                                                                      atCGTCTTGTGTCGAAACGCCCCG‑CCGCAGGCTTGCCGCGCGGATGGCATTGAGATCGGTGGCTGGCAAATCTTCCTGCCCCAAATGGATGCCATACGCCTGATTTGTTATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTGATAGCGGCGGCGCAGCGCAATTGCCGCCACGACATCGGCTTCCACCGCTTCAGCGCGCCGATCTTTGATGCGGATCTGGATAGTCCGTACGTCTGCATcc                                                                                                                                <  2:374213/250‑1 (MQ=255)

                                                                                                                       ccGTCATGTGTCGAACCGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGACCGGTGGCTTGCAAATCCTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCa‑a                                                                                                                             <  2:76518/250‑1 (MQ=255)

                                                                                                                        cGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGcc                                                                                                                                                                         >  1:387939/1‑207 (MQ=255)

                                                                                                                         gTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑Ac                                                                                                                            >  1:433119/1‑250 (MQ=255)

                                                                                                                           cATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATATTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAgc                                                                                                                         >  2:556437/1‑250 (MQ=255)

                                                                                                                            agggggcGAAAC‑CCCAGGCCGCAGGCTTGCCGCGCGGAGGGCATTGAGATCGGTGGCTTGCAAACCTTCCCGCCCAAAATGGCCGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGGAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGAc                                                                                                                        <  2:106819/245‑1 (MQ=255)

                                                                                                                                  cgcaacggccgatcagCAGGACTGCGGCGCGGATGGCATTGGGATCGGTGGCTTGCAAATCTTCCGGCCCCAAATGGCTGCCACACGCATGATGCGTAATCGCCAGCCGCCAGTAAGAGTTGATAAACAACCGCGCGTTATAGCGGCGGCTCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTGCATCGCGCCGATCGGTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACCGACGTTCg                                                                                                                   <  2:104073/236‑1 (MQ=255)

                                                                                                                                   gatccGCCCAG‑CCGCAGGCCGGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCAAAATTGACGCCATACGCCTGATGCTTGAGCGCCAGCCGCCAGTAAGCGTGGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGAt                                                                                                                 <  2:375047/247‑1 (MQ=255)

                                                                                                                                       cGCCCAC‑CCGCAGGCCTGCCGCGCGGATTGCTTTGAGTGCGGTGGTTTTCAAAGCTTCCTGCCCCAAATGGCTGCCATCCGCCTGTTGTTTGATCCCCGTCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGATAGTACGTACGCGTGCATCCA‑ACAGACGTTCGATCCAc                                                                                                             <  2:504454/251‑1 (MQ=255)

                                                                                                                                        gCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACt                                                                                                            <  1:215182/251‑1 (MQ=255)

                                                                                                                                         cccAG‑CCGCGGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTg                                                                                                           <  1:32565/251‑1 (MQ=255)

                                                                                                                                          ccAG‑CCCCAGGCCTGCCGCGCGGATGTCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCAATACGCCGGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGAGCCACTGt                                                                                                          <  2:204372/251‑1 (MQ=255)

                                                                                                                                             cgcggCAGGCCTGCCGCGCGGATGGCATGGAAACGGGTGGTTTGCAAATTTCCCTGCCCCAAAGGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTAc                                                                                                        <  2:51969/247‑1 (MQ=255)

                                                                                                                                                cGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTGGCAAATCTTCCTGCCCCAAATGGACGCAATACGCCTGATGCTTGATCGCCAGCCGCCAGTACGCGTTGATAAACACTCGCGCGTTATACCGGCGGCCCAGCGCAATTGCCGCAACGCCATCGGCTTCCACCTCTTCATCGCGCCGCTCTTTGATGCGTCGCTGGCGAGTACGTACGACTGCATACA‑AACGAAGTTCGATCCACTGTCCGCt                                                                                                     >  2:79258/1‑251 (MQ=255)

                                                                                                                                                cGCAGGCCTGCCGCGCGGAGGGCATTGAGACCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGCCGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCt                                                                                                     <  1:430846/251‑1 (MQ=255)

                                                                                                                                                      ccggCCGCGCGGATGGCATTGAGAGCGGTGGCTGTCAAAGCTTCCTGCCCCAAATGGACGCCATACGCCGAATTCTTGAGCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACGGTACGCTGTccac                                                                                               <  2:523761/248‑1 (MQ=255)

                                                                                                                                                        tGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTccacc                                                                                              >  1:535606/1‑250 (MQ=255)

                                                                                                                                                                 gATGGCATTGAGATGGGTGGTTTGCAAATCTTCCGGCCCCAAAGGGACGCCATCCGCCTGATGTTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCGTTGATGCGTAGCTGGAGAGTTCGTACGCCTGCATCCA‑ACAGACGGTCGATCCACGGTACGCTTTCCACCTCCGGGTACa                                                                                    <  2:397228/251‑1 (MQ=255)

                                                                                                                                                                 gATGGCATTGAGATCGGTGGCTTCCAAATCTTCCTGCCCCAAAGGGACGCCATCCGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACa                                                                                    <  1:272248/251‑1 (MQ=255)

                                                                                                                                                                   tGGCATTGAGACCCGTGGCTTGCAAATCTTCCTGCCCCAACTGGACGCCATACGCCTGATGCTTAATCGCCAGCCGCCTGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGACCAGCGAAATTGCCGCCACGACATCGGCTTCCAACTCTTCATCGCGCCAATCGTTGGTCCTTAGCTGGCGAGGACGACCGACTGCATCCACACA‑ACGGTCTATCCACTGTATGCCGTCCACCACCGGGTAccat                                                                                  >  2:530345/1‑248 (MQ=255)

                                                                                                                                                                         ggagaTCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGaa                                                                            <  1:244269/250‑1 (MQ=255)

                                                                                                                                                                             aTCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACTCCATGCGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGaa                                                                        <  1:9268/251‑1 (MQ=255)

                                                                                                                                                                              tCGGTGGCTTGCAAATCTTCCTGGCCCAAATGGACCCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGAAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTACATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGaa                                                                        >  2:313440/1‑250 (MQ=255)

                                                                                                                                                                              ccGGTGGCTTGAAACGCTTCCTGCCCCAAATGGACCCCATACGCCTTATGCGTGATCGCCGGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCACCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGaa                                                                        <  2:346252/249‑1 (MQ=255)

                                                                                                                                                                               cgttGGCTTGCAAATCTCCCCCCCCCAAAGGGGCGCCATACGCCTGATTCTTGATCGCCGTCCGCCAGTAAGCGTTGATAAAAAATCGCGCGTTAGAGCGGCGGCCCAGCGCAATTGCCGCCACGACAGCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCCCCGGGTACAGTCCTGAACGaaaa                                                                      <  2:438189/248‑1 (MQ=255)

                                                                                                                                                                               cggtggttggcaatcctcccggCCCCAAAGGGACGCCATACGCCTGATGTTTGTTCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGaaaa                                                                      <  2:161568/230‑1 (MQ=255)

                                                                                                                                                                                ggTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGaaa                                                                       >  1:249271/1‑249 (MQ=255)

                                                                                                                                                                                ggTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAg                                                                     <  1:589648/251‑1 (MQ=255)

                                                                                                                                                                                   ggCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGCCATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCCCCACCGGGTACCGTCCTGAACGAAAAGGt                                                                   >  2:612991/1‑250 (MQ=255)

                                                                                                                                                                                      ttGCCAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATAGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGAGTTATCGCGGCGGCCCAGCGCAATTGCCGCCACGACCTCGTCTTCCACCTCTTCCTCGCGCCGGTCTTTGATGCGTAGCTAGCGAGTGAGTACGCCTGCAGCCA‑ACAGACGTTCGATCCGCTGTAAGATCTCCACCACCGGGTACAGTCCTTAACGAAAACGTAc                                                                 >  1:613807/1‑249 (MQ=255)

                                                                                                                                                                                         cAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACaggag                                                            <  1:470829/251‑1 (MQ=255)

                                                                                                                                                                                          aaaTCTACCAGCCCCAAATGGAAGCCATACGACAGATGCTTGATCGCCAGCAGCCAGAAAGCGTTGATAACCAACCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGCTCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATACA‑ACAGAAGTTCGATCCACTGTACGCTGTCCACCACCGGGTACCGTCCTGAACGAAAAGGGACcggagg                                                           >  2:423145/1‑251 (MQ=255)

                                                                                                                                                                                             tCTTCCTCCCCCAAATGGACGACATCCGACTGATACTTGATCGCCAGCCTCCAGTAAGCGTTGATAAAAAATCGCGCGTTATAGCCGCGGCCCAGCGCAATTGACGCCACGACCTCGGCTTCCACCTCTTCCTCGCGCCGTTCATTGATGCGTAGCTGGAGCATACGGACGCCCGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGACCACCACCGGGTACAATCCTGaaataaaacgtaccggaagaa                                                         >  2:356774/1‑231 (MQ=255)

                                                                                                                                                                                                ccccgCCCCAAATGGACGCCATACGCCTGATGTTGGATCGCCAGCCGCCAGTAAGCGTTGAAAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATc                                                     <  2:157154/247‑1 (MQ=255)

                                                                                                                                                                                                    gCCCCAAAGGGACGCCATCCGCCTGATGCTGGCGCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGTTTCCACCTCGTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGGACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAg                                                   <  2:208129/249‑1 (MQ=255)

                                                                                                                                                                                                            tGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTTATGCTTAGCTGGAGAGTCCGTACTCCTGCCTCCA‑ACAGACGTTCGATCCCCTGTACGCTGTCCACCACCGGGTACCGTCCTGAACGAACAGGTACAGGAGGAAAATCCTGCTGATACA‑t                                        >  1:360228/1‑251 (MQ=255)

                                                                                                                                                                                                              gACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGCTCTTTGATGCGTAGCTGGAGAGTACGTAAGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACCGGAGGAAAATCAGGCTGATACA‑Tc                                       >  1:237216/1‑250 (MQ=255)

                                                                                                                                                                                                                cGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACg                                    <  1:113543/251‑1 (MQ=255)

                                                                                                                                                                                                                  ccatcccccTGAGGCTTGACCGCCCGCCGCCAGTAAGCTTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACGCt                                  <  2:609173/244‑1 (MQ=255)

                                                                                                                                                                                                                  ccATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACGCt                                  <  2:28995/251‑1 (MQ=255)

                                                                                                                                                                                                                    aTACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAACCAATTGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACGCTtc                                >  1:151011/1‑251 (MQ=255)

                                                                                                                                                                                                                      aCGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGCTGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACGCTtcct                              >  1:593499/1‑251 (MQ=255)

                                                                                                                                                                                                                       cgcccgctgttgtagcgcccgccGCCAGTAAGCGTTGATAAACAATCGCGCGTTAGGGCGGCGGCCCCGCCCAATTGCCGCCGCGTCATCGGCTTCCACCTCTCCACCGGGCCGATCTTTGATGCGTCGCTGGAGAGTACGTACGCGTGCATCTA‑ACAGTCGTTCGAGCCGCGGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑GCACGCTtcctc                             <  2:335325/236‑1 (MQ=255)

                                                                                                                                                                                                                        gccggaggCTTGAGCGCCAGCCGACAGTAAGCGTGGATAAGCAATCGCTCGTAATAGCGGTGGCCCAGGGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGAGCTTGGATGCGTAGCTGGAGAGGACGGACGCCTGCAGCCA‑ACTGACGTACGAGCCACTGTACGCGGTCCTCCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACGCTtcctc                             <  1:260185/243‑1 (MQ=255)

                                                                                                                                                                                                                        gCCTGATGCTGGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACGCTtcctcc                            <  2:545747/251‑1 (MQ=255)

                                                                                                                                                                                                                        gCCTGATGCATGAACGCCAGCCGCCAGGAAGCGTTGATAAACAATCGAGCGTTATAGCGGCGGCATAGCGCAATTGCCGCAACGACATCGGATTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑AAAGACGTTCGATCCACTGTACGCTGTACACCACCGGGTACAGTCCTGTACGAAACGGTACAAGAGGAAAATCAGGCTGAT‑CACTCACGCTtcctcc                            >  2:44257/1‑251 (MQ=255)

                                                                                                                                                                                                                        cccgtaTGCTTGATCGCCGGCCGCCAGTAGGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACGCTtcctct                            <  2:384405/246‑2 (MQ=255)

                                                                                                                                                                                                                            ggtgcttgtacGCCAGCCGCCAGTAAGCGTTGAAAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCTCGTCATCGGCTTCCTCCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGAGGTTCGATCCACTGTACGCTTTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCGGATACA‑TCACGCTTCCTCCTTAc                        <  2:261834/241‑1 (MQ=255)

                                                                                                                                                                                                                            gATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTAAAGTCCTGAACGCAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TAACGCTTCCTCCTTAc                        >  2:218611/1‑251 (MQ=255)

                                                                                                                                                                                                                            gATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATGGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACGCTTCCTCCTTAc                        <  1:532159/251‑1 (MQ=255)

                                                                                                                                                                                                                            gATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTAGAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACGCTTCCGCCTTAc                        <  1:550251/251‑1 (MQ=255)

                                                                                                                                                                                                                                    aTCGCCAGACGCCAGTACGCGTTGATAACCAATCACACCTTATACCGGCGGCTCAGCGCAATTGCCGCCCCGACATCGGCTTCCCCCTCTTCATCGCGCCGCACTTTGCTGCGTAGCTGGAGTGTACGTACGCCAGCATCCA‑ACAGACGCTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATAAGGATGCTACA‑TCAAGCTTCCACCTTACGCAggc                  >  2:590240/1‑248 (MQ=255)

                                                                                                                                                                                                                                          aGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGCAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGCGGAAAATCAGGCTGATACA‑TCACGCTTCCTCCTTACGCCGGTAGATTtct          >  2:46029/1‑251 (MQ=255)

                                                                                                                                                                                                                                                  gTAAGCGTTGCTAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGCTCTTTGATGCGTAGCTGGCGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACCGTCCTGAACGACAAGGTACAGGAGGAAAATCAGGCTGATACA‑TAACGCTTCCTCCTTACGCAGGCAGATTTCTCCGCctct  >  2:296633/1‑251 (MQ=255)

GCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAG‑CCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCA‑ACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACA‑TCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCT  >  NC_000913/4193751‑4194242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`