| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,133,252 | C→T | P213S (CCG→TCG) | metF → | 5,10‑methylenetetrahydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,133,252 | 0 | C | T | 96.3% | 143.2 / NA | 54 | P213S (CCG→TCG) | metF | 5,10‑methylenetetrahydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (18/34); minor base G (1/1); total (19/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
ATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGA > NC_000913/4133007‑4133494 | aTGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATGCCGGGAATTTTGTCGGt < 1:562484/250‑1 (MQ=255) tCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTa < 1:148250/250‑1 (MQ=255) aCCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGAT‑GTGG‑GAATTATTTCGGGGATTTTGTCGGTATCTAACttt > 1:278037/1‑251 (MQ=255) gggTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCGCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTTCCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑aaa < 2:79171/250‑1 (MQ=255) acccTGTGAAAAGAAGTGTCAGATTACGATTTTTCCGGGGCGGCGGAACCGGAAGTTCACCCGTAAGCAAAAAGCGCTCAGGCGAAATGGCTTAATCTGAAACTCAAAGGGGATGCCGGAGCCAACCGCGCGATTCCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTGTGTCTTTATCTAACTTT‑AAACatgc < 2:27094/248‑4 (MQ=255) aCGCTGTTAAAAGAAGTGGCAGAGTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACGTT‑AAACAGG‑c < 2:258128/251‑1 (MQ=255) gCTGTTAAAAGAAGTGGCAGATTTCGATAACTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTAGTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑Cga < 2:189647/251‑1 (MQ=255) ggtAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑Cgaag < 1:544108/248‑1 (MQ=255) tAAAAGAAGTGGCAGAGTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCGGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑Cgaagaa < 2:559863/250‑1 (MQ=255) gCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTTGT‑GTGG‑GAATTTTTCCGGGGATTTTGTTGGTATCTAACTTT‑TAACAGA‑CGAAGAAAATTTGccatat > 1:398894/1‑247 (MQ=255) gATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAc < 1:74015/251‑1 (MQ=255) cGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAAc < 2:182679/250‑1 (MQ=255) gATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCGAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGt < 1:99489/251‑1 (MQ=255) ggcggcGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCGCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGGCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCgg < 2:373586/251‑1 (MQ=255) ggcggaGTATACGGAAGTGCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTGGCCGATATGAC‑Caatgggggtatttcgg < 2:324195/251‑15 (MQ=255) ggAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATc < 1:587728/186‑1 (MQ=255) aGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTAAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGTTGTCGAAAGCTACCTGAGATTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑GAATTTTTACGGGAATTTTGTCGGTATATAACTTTCAAAC‑GG‑CGGAGAAA‑TTTTCTGATATGACACAA‑GTGCGTATTCCGGCGTgggaggggcca > 1:419457/1‑241 (MQ=255) ttCACCCGGAAGCAAAAAGCGCTCAGGCGGCTTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGTT‑GTGG‑AAATTTTTTCGGGGATTTTTTCGGGATCTCACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGCTATGAA‑CAACGTGCGTATTTCGGCGGGGGTGGGGCaactg > 1:543577/1‑248 (MQ=255) ttCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGACACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑CAATTTTTTCGGGGGTTTTGTCTGGATCTAACTTT‑TTACAGAAGGAAAAAA‑TTT‑ACGATATGAA‑CAACGTGCGTTTTCCCTCGAGGATGGCGCCAATg > 2:273433/1‑251 (MQ=255) cGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTTTTTCGGGGATTTTTTCGGTATCTATCTTT‑TAACAGG‑CGAAGAAA‑TTTTCCGATATTAC‑CCAAGTGCGTATTCCGGCGTGGCTGGGGCGAATATTCGAc > 1:349978/1‑251 (MQ=255) aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGTT‑GTGG‑GAATTTTTTCGGGGATTTTGTCGGTGTCTAAATTT‑TAACAGG‑CGAAGAAA‑ATTGCCGATATGAC‑CAACATGCGTATTCCGCTGTGGCGGGGGCAAATGTTctgcggggtgg > 2:48081/1‑241 (MQ=255) aaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCACAGTGGATGCCGGAGCCAACCGCGCTATTACTCAGTTCTTCTTCGAGGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCTGCGTGGATGGCGCAAATGTTCGACGGTCTGga < 1:520628/251‑1 (MQ=255) aaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑GAATTTTTTCGGGGATTTTTTCGGTTTCTAACTTT‑TAACAAG‑CGGAGAAA‑ATTTCCGATATGAC‑CCACGTGCGTATTTCGGCGTGGATGGGGCAAATGTTCGGCGGTCTgggtg > 1:118001/1‑248 (MQ=255) aGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGcc < 1:67107/251‑1 (MQ=255) ttAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGAc < 2:173556/221‑1 (MQ=255) ttAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGGATCTAACTTT‑AAAAAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTTCGGCGGGGATGGCGCAAATGTTCGAc > 1:173556/1‑221 (MQ=255) tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGGTTTGTAGCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATGTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGAGATGTC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCa < 1:568628/248‑1 (MQ=255) aaaGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCGTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATGATTCCGGGAATTTTGTCGGTATCTAACGTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTTTATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAATCTGGTGTGCGCGaa < 2:236570/251‑1 (MQ=255) tGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGGATCTAACTTT‑AAACAAG‑CGAAGAAA‑TTTTCCGATATGAC‑CAACGTGCGGATTTCGGCGTGGATGGCGCAAATGTTCGACGGGCTGGGTGATGATGCCGAAACCCGCAAACAGGTTGGCGCGGATAtt > 1:18511/1‑251 (MQ=255) ggAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAGTGAT‑GTGG‑GAATTATTTCGGGGATTTTGTCGGTATCTAACTTT‑TAACAGG‑CGAAGAAA‑TTTGTCGATATGAC‑AAACCTGCGTATTCCGGCGGGGGTGGCGCCAATGTTCGACGGTCTCGGTGATGGTGCCGAAACACCCAAAATGGTTGGCGCGGATCTTGcacagg > 1:355376/1‑245 (MQ=255) aacggcgcgATTACTCATTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGGCGGTCTGGATGATGATGCCGAAACCCGCAAACTGGGTGTCGCGAAGATTGCCATGGATATGGt < 2:52479/247‑1 (MQ=255) cAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGc < 1:244140/249‑1 (MQ=255) gTTCTTCGTCGATGTCGAAAGCTACCTGCGTTGTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTAGTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTg < 2:93834/251‑1 (MQ=255) tcttcGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTAGCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGaa < 1:356388/248‑1 (MQ=255) tcGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAg < 1:169671/249‑1 (MQ=255) aTGTCGAAGGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGGT‑GGGGGAATTTTTTCCGGGA‑CTTTGTTGGGATCTTAATTT‑TAACAAG‑CGGAGGAA‑TTTTACCATATGAA‑CAAAGTGGGTATTTCTGCGGGGGTGGGGCAAAAATTCGCCGCTCTTGATGATGGTGCCGAAAACCCCCAACTGGTTGGCGCGAAGAATGCCATGGCTTTGGTGGAGCATTTAAATCGTGAAAGAGTgga > 2:544560/1‑248 (MQ=255) aTGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGGTTGTTTGGGG‑AAAT‑ATTCCGGGAATTTTTTTGGGTTCTCACTTT‑AAACAGG‑CGCAGAGA‑TTTGCCGATATGAT‑AAAGGTGCGTATTTCGGCGGGGCAGGCGCAAAAATTTGGCGGTCTGGGGTATGATACAGAAAACCGCAAACTGGTTGGCGCGAATATTGCCATTGATATGGTGGAAATTTTAAACCggggaagagggac > 1:299705/1‑238 (MQ=255) gTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGAGATGGTAAAGATTTTAAGCCTTCAAGGATTGAAAGa < 2:40971/251‑1 (MQ=255) gCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCAc < 2:114733/251‑1 (MQ=255) aCCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACt < 2:278037/249‑1 (MQ=255) gTTTTCGTGACCGCTGTGTATCGGCGGGCATTTGT‑GTGG‑AAATTATTTCGGGGATTTTTTCGGGTTCTAAATTT‑TAAAAAG‑CGAAGAAA‑TTTTGCGATATGAC‑CCACATGCGTATTTCGGCGGGGGTGGGGCAAATTTTCGACGGGCTGGGTGATGATTCCGAAAACCGCAAACCGGTTGGGGGGGATATTGCCATGGGTATGGGGAAGATTTTTAATCGTGAAGGAGGGAAAGAATTACACTTTTATACg > 2:322258/1‑251 (MQ=255) cggcggGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTGT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAg < 2:536512/250‑1 (MQ=255) cggGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTAc > 1:172666/1‑251 (MQ=255) ggCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTAcgc < 2:118001/251‑1 (MQ=255) ggCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTAcgc < 2:453606/251‑1 (MQ=255) ggCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTAcgc > 1:41757/1‑251 (MQ=255) gtgG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGcc < 1:445828/250‑1 (MQ=255) tgG‑AAATTATTCCGGGAATTTTGGAGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGGGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAAACGTGCTGAAATGAGTTACGCGATTTGCCAt > 2:307642/1‑251 (MQ=255) g‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATAc < 2:349978/251‑1 (MQ=255) ttattCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTc < 2:119954/98‑1 (MQ=255) ttattCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTc > 1:119954/1‑98 (MQ=255) tCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTggggg > 2:370911/1‑251 (MQ=255) ccGGGAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGt < 1:20368/251‑1 (MQ=255) gAATTTTGTCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTGGCCATACGCTGGGGGTTCGa < 1:203013/251‑1 (MQ=255) | ATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGG‑AAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGG‑CGAAGAAA‑TTTGCCGATATGAC‑CAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGA > NC_000913/4133007‑4133494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |