Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,991	G→A	L5L (CTG→TTG)	asnC ←	DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,991	0	G	A	100.0%	211.0 / NA	67	L5L (CTG→TTG)	asnC	DNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/42);  total (25/42)

GCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGG  >  NC_000913/3926748‑3927237                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        gcgcGGAAGGGTAGTCTTTGGCGCTCTTTAATAAAATGCCGATAAAGCAGCTTA‑CGTCATAACCGAGTTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAAttt                                                                                                                                                                                                                                                  <  1:217772/251‑1 (MQ=255)  cgcgGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGTTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAAtttt                                                                                                                                                                                                                                                 <  1:402699/251‑1 (MQ=255)     ggAAGGGTAGTCTTTGGCGCTCTTTAAGATAATGCCAATAAAGCCGCTTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATCCGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCa                                                                                                                                                                                                                                              <  1:505970/251‑1 (MQ=255)       aaGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATACAGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAACTTGTTTCGCCAGTTCGGAGTAAGCGGTGCGCGCATTGCCCATTCATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCAt                                                                                                                                                                                                                                             >  2:381327/1‑250 (MQ=255)        aGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTC‑CGTCATAACCGAGCTGCTTCGGGTTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAg                                                                                                                                                                                                                                           <  1:72174/251‑1 (MQ=255)           gTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTG‑CGTCATAACCGGGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGACCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGAt                                                                                                                                                                                                                                        <  1:14263/251‑1 (MQ=255)             agtttttGGCGCTTTTGAATATAATTCCGATAAAGCACCCTC‑TGGCATACCCGGGCTGTTTCGGGTGGACATCAATACGCGCCCCGGTAATGACCCCCGCCTGCTTCATTTTCTCTACGCGAACGTTAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTGCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGAGTGTCGATCAAATAAGTTGCCATAGGAttt                                                                                                                                                                                                                                      <  2:425367/247‑1 (MQ=255)             aGTCTTTGGGGCTCTTTAATATAATGCCGATAAAGCAGCCTC‑GGTCAAAACCGAGCTGCTTCGGGCTGACAGCAATCCGCGCCCCGGGAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTGCGGCGTAAGCTGTGCGCGCATTGTCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGAttt                                                                                                                                                                                                                                      <  2:357097/251‑1 (MQ=255)                   tGGCGCTCTTTAATATAATGCCGCTAAAGCAGCCTA‑CGTCATAACCAAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCTGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATACTTTTCCATAGGATTTTCTTAt                                                                                                                                                                                                                                >  2:267172/1‑251 (MQ=255)                     gcgcTCTTTAATATAATGCCGATAAAGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGc                                                                                                                                                                                                                              <  2:379678/251‑1 (MQ=255)                       gcTCTTTAATATAATGCCGATAAAGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGACTCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCgg                                                                                                                                                                                                                            >  2:86036/1‑251 (MQ=255)                        atctTTAATATAATGCCGATAAAGCAGCCTA‑CGTACTAACCGCGCTGCTTCGGTCTGACATAAATACGCGCCCCGGTAATGATCCCCGCTTGCTTCATTTTCTCTACTCGCACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCTCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATGTTCGATCAAATAATTTTCCATAGGATTATCTTATGCgg                                                                                                                                                                                                                            >  2:137236/2‑250 (MQ=255)                            ttAATATAATGCCGATAAAGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATtg                                                                                                                                                                                                                        <  1:148664/250‑1 (MQ=255)                            taaaaataATGCCGATAAAGCAGC‑TATCGGCATACCCAAGCTTCTCCGGGCTGACTTCAATACGCGCCCCGGTATTGAGCCGCGCCTGCTTCATTTTCTCTACTCGAACGTTAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTGGATCGAATAATTTTCCATAGGATTTTCTTAttcgtatt                                                                                                                                                                                                                         <  2:297303/244‑8 (MQ=255)                              aaTATAATGCCGATAAAGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATtgatg                                                                                                                                                                                                                     >  1:236238/1‑251 (MQ=255)                               atatAATGCCGATAAAGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATtgatg                                                                                                                                                                                                                     >  1:461010/1‑250 (MQ=255)                                 ataATGCCGATAAAGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGatt                                                                                                                                                                                                                  >  2:453997/1‑251 (MQ=255)                                          aTAAAGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCtctt                                                                                                                                                                                                         >  2:496031/1‑248 (MQ=255)                                            aaaGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTAtttt                                                                                                                                                                                                       >  2:478450/1‑251 (MQ=255)                                               gcagcCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGCTGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGc                                                                                                                                                                                                    >  1:434338/1‑251 (MQ=255)                                                          cgaaacccGAGCTTCTTCGGGCTGACTACAATACGCCCCCCGGAAATGTTCCCCTGCTGCTTCAATTTCTCTTCTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTTGTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCAGTCTATTTTAGCCTTCtttttt                                                                                                                                                                                          <  2:132581/245‑1 (MQ=255)                                                           cctaacctgtctgctTCGGGCTGCCAGCAATACGCGCCCCGGTAAGGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGTATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTa                                                                                                                                                                                         <  1:375341/241‑1 (MQ=255)                                                                 cGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAAt                                                                                                                                                                                   >  2:477739/1‑251 (MQ=255)                                                                  gAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATc                                                                                                                                                                                  <  1:194413/251‑1 (MQ=255)                                                                     ctgctTCGGGCTGACATCAATACGCGCCCCGGTACTGACCCCCGCCTGCTTCCTTTTCTCTACTCGAACGTGAATCGACCCCGGCCTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCCGATTGTCGATCAACTAATTTTCCATAGGATTTTCTTATGCGGCTtg                                                                                                                                                                                                                        >  2:406972/1‑210 (MQ=255)                                                                     ctgctTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATtg                                                                                                                                                                                                                        <  1:406972/210‑1 (MQ=255)                                                                     ctgctTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCaa                                                                                                                                                                                >  1:376788/1‑250 (MQ=255)                                                                       gtttCGGGCTGCCATCAAATGGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGAGCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTGTTTTAATGAATCaaaa                                                                                                                                                                              <  1:445633/248‑1 (MQ=255)                                                                       gctTCGGGCTGACAGCAATCCGCGCCCCGGTAAAGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGAATGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAg                                                                                                                                                                             <  1:7229/251‑1 (MQ=255)                                                                               cTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGtt                                                                                                                                                                       >  1:589298/1‑249 (MQ=255)                                                                                tGACATCTATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGCACGTGAATCGTCCCCGGCCTGACGCCACATTGTCTCGCCAGTTCGGCGTAAGAGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTa                                                                                                                                                                      >  2:370230/1‑249 (MQ=255)                                                                                 gACATCAATACGCGCCCCGGGAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTGTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAg                                                                                                                                                                     <  1:294723/249‑1 (MQ=255)                                                                                            gcgcCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCATCTGTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGaa                                                                                                                                                        <  2:272216/251‑1 (MQ=255)                                                                                             cgcCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATGGCCCAGTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAAt                                                                                                                                                       <  1:255673/251‑1 (MQ=255)                                                                                                cccGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGat                                                                                                                                                    <  1:478450/251‑1 (MQ=255)                                                                                                      aaTGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGc                                                                                                                                              >  1:328785/1‑251 (MQ=255)                                                                                                                    gCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGGGTCGGtt                                                                                                                                 <  2:324356/250‑1 (MQ=255)                                                                                                                    gCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAAGTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGtt‑t                                                                                                                               <  1:380910/251‑1 (MQ=255)                                                                                                                    gCTTCATTTTATCTACGCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGAGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTGTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGCCGGtt                                                                                                                                 <  1:339569/250‑1 (MQ=255)                                                                                                                           tttCTCTACTCGAACGTGAATCGTACCCGGACTGACGCCAAATTGTTGCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTGTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑Tttgttg                                                                                                                         <  2:120874/250‑1 (MQ=255)                                                                                                                                ctACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTa                                                                                                                     <  1:587438/249‑1 (MQ=255)                                                                                                                                     cGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCCGTTCGGCGTAAGCGGTGCGCGCAGTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGTCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCAGGTGTGTCGGTT‑TTTGTTGCTTAATCATa                                                                                                               <  2:307058/250‑1 (MQ=255)                                                                                                                                        aCGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGc                                                                                                            >  1:355356/1‑250 (MQ=255)                                                                                                                                          gTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCGTCTTTTTGAATGAATCAAAAGTGAGTTAGGCGTTTTATTGAATGATGATTGCATTTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAAc                                                                                                         <  2:316398/251‑1 (MQ=255)                                                                                                                                             aaTCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAgg                                                                                                      <  1:239421/251‑1 (MQ=255)                                                                                                                                                cGTCCCCGGACTGACGCCAAATTGTTGCGCCTGTTCGGCGTAAGAGGTGCGCGCATTGCACATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAGTTTTCCATAGGATTTTCTTATGCGGATAGATGATTCATTCTATTTTAGCCTTCTTGTCTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCAAGTGTGT‑GTTTGTTAGTTGCGTAATCATAAGCAACAGGACg                                                                                                   <  2:361729/251‑1 (MQ=255)                                                                                                                                                                           cGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGATTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTA‑ATTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAGACCGcgt                                                                        <  2:205679/251‑3 (MQ=255)                                                                                                                                                                           cGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCt                                                                         <  1:322012/250‑1 (MQ=255)                                                                                                                                                                                       gTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaac                                                            >  1:10037/1‑251 (MQ=255)                                                                                                                                                                                       gTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaac                                                            >  2:502859/1‑251 (MQ=255)                                                                                                                                                                                         aaGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaacaa                                                          <  1:115514/251‑1 (MQ=255)                                                                                                                                                                                         aaGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaaca                                                           <  1:439014/250‑1 (MQ=255)                                                                                                                                                                                             ggTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAAACGCTTACATTGCCAAACAACGTc                                                      >  1:625343/1‑251 (MQ=255)                                                                                                                                                                                                gcgcgcATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCaaa                                                   <  1:198529/251‑1 (MQ=255)                                                                                                                                                                                                    gcATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAAtt                                                 >  2:495027/1‑249 (MQ=255)                                                                                                                                                                                                    gcATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAg                                               <  2:119425/251‑1 (MQ=255)                                                                                                                                                                                                           ccATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTg                                        <  1:267172/251‑1 (MQ=255)                                                                                                                                                                                                           ccATTAATGCATCAAGGATGCCACGGACCAGATTGTCGCTCAAATAATTTTCCATAGGCTTTTCTTATACGGATTGATGATTCATTATATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGCGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGCCGCAGGAGTATAAAAACTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTg                                        >  2:599748/1‑251 (MQ=255)                                                                                                                                                                                                            cATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAGATTAGCTTCGTGa                                       <  2:252484/251‑1 (MQ=255)                                                                                                                                                                                                                           gattccACGGTCCAGATTGGCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTT‑AATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCg                       <  1:564801/247‑1 (MQ=255)                                                                                                                                                                                                                               ccACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCa                    <  1:453997/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTAGTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGga              <  1:380456/250‑1 (MQ=255)                                                                                                                                                                                                                                        aGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGgaaga           <  2:466317/251‑1 (MQ=255)                                                                                                                                                                                                                                             gTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTc      >  1:352156/1‑251 (MQ=255)                                                                                                                                                                                                                                               cGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAATAACGTCt     <  2:376788/250‑1 (MQ=255)                                                                                                                                                                                                                                                 aTCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTggg  >  1:266211/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aTCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTggg  >  1:243881/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        GCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTA‑CGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTT‑TTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGG  >  NC_000913/3926748‑3927237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑