| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,773,176 | C→T | S299F (TCT→TTT) | mtlA → | mannitol‑specific PTS enzyme IICBA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,773,176 | 0 | C | T | 97.2% | 340.4 / 0.9 | 109 | coding (896/1914 nt) | mtlA | mannitol‑specific PTS enzyme IICBA component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (55/48); minor base . (3/0); total (58/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
TGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACGT > NC_000913/3772931‑3773414 | tGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGATAAACCGTCTGCGGGCGGTGCGGCACTCATCCACTTCCTGGGGGGCATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGATCACGCTGACTATCCTGGGCGGAGGACTGGTTTCTCCGGCATCTCCGG‑TTTTATCCt > 1:53717/1‑251 (MQ=255) gAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCtt > 1:214451/1‑251 (MQ=255) aaGCTAACCCATGTCCAGGTATGGGCGTGCTGCTGGCGTACATGGTCTTTGGTCGGGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACTAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGTGGTATGACTGGCGTGTTCACGCTGACTATCCTTGGCGGTGGGCGGGTTTCGCCGGCAACTCCGG‑TTTTATCCTTg < 2:89489/251‑1 (MQ=255) aaGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCGTTGGGCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGAGCCTCGCAGTCATCCTCGGCGGTATGACTGTCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTg < 2:368229/251‑1 (MQ=255) tAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTTTCCTTGCTGt > 2:472566/1‑251 (MQ=255) ccaggtccaggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGCCTGGCGTGTTCAAGATGACTATCCTGGGCGGTGGGCTGGTTTCTCCGGCATCTCCGG‑TTTTTTCCTTGCTGCACTg > 1:294162/1‑251 (MQ=255) ggtccaggtATGGGCGTGCTGCTGGCGTCCATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCCATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTTTCTTTGCCGTACTGGCg > 2:48808/1‑251 (MQ=255) ggtccaggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGGGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTCCTGGCg > 2:364053/1‑251 (MQ=255) ccggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCCCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTCCTGGCgctg > 2:449394/3‑247 (MQ=255) caggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGa < 1:153434/251‑1 (MQ=255) gttATGGGCGTGCTGCTGGCGTACATGTTCTTGGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGaca < 1:414924/249‑1 (MQ=255) gtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGTTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGaccc > 2:160130/1‑249 (MQ=255) gTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTATCGCTACACAGTCTGCGGGCGGAGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGCGTTCCCGCTGACTATCCTGGGCGGGGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAACAggg > 2:245777/1‑250 (MQ=255) ttGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TGTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTACGCTAACATcg < 2:414212/248‑1 (MQ=255) tgggTCGTGGTAGCGCTAATCAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTAGCCACGAAATCTTCTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCGGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTGTATCCTTGCTGTACTGGCGATGACCCCAAATTGTGCTTACTTCGCTGACATcg < 2:113411/246‑1 (MQ=255) tGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTTTCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCggg > 1:325919/1‑251 (MQ=255) gTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGgtg > 1:583769/1‑251 (MQ=255) ggTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGgtgtgtgg > 1:216466/1‑250 (MQ=255) gcTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGGCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgcggcg > 1:47322/1‑251 (MQ=255) cggcgaacaactatgactggagCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGAGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATACTGGGCGGGGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGAAGACAACAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgcggcg > 2:627261/22‑251 (MQ=255) aaCAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATgg < 1:112165/251‑1 (MQ=255) gcggcgggcggTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCGGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGtc < 2:114588/248‑1 (MQ=255) cTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCCTCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGGGTGTGTGCGGCGATGGCTggct > 2:614140/1‑248 (MQ=255) gggcggTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCtt > 1:592922/1‑251 (MQ=255) atcatcCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCt > 1:172944/1‑251 (MQ=255) cacgtCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTg < 1:505035/247‑1 (MQ=255) cACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTTGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGGGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTg > 1:454606/1‑251 (MQ=255) cACTTCATGGGGGGCATCCACGCGATCTACTTCCCGTATCTGCTGATGAATCCGCGTCTGACCCTCACAGTCATCCTCGGCGGTATAACTGGCGTGTTCCCACTGACTATCCTGGGCGGTGGTCTGGTTTCCCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCCAAAGGTGCTTACTTCGCTTACATCGCGGGTGTGGGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTCTTTTg > 2:256096/1‑251 (MQ=255) aCTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGc > 1:563570/1‑251 (MQ=255) cTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGaa > 1:216253/1‑250 (MQ=255) ggggggTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCGCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGaaaa < 2:287203/250‑1 (MQ=255) gggggTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGAGATCACGCTGAATATCCTGGGCGGTGGTCTGGGTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTAGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAAcc > 1:269886/1‑251 (MQ=255) gtctCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCTCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCGGGGCTGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCGTACTGCGCTAACATCGCGGGTGTGTGTTCGGCGATGGCTGTCTCCGTCTTTTTCTCTGCTATTTTGCTGAAATCCAGCa < 2:418213/248‑1 (MQ=255) ggaTCCACGAAATCTACTTCCCGTATGTGCGGATTAATCCGCGTCTGATCCTCGCAGTCCTCCGCGGCGGTAGGACTGGCGTGTTCACGCTGATTATCCTGGGCGGTGGTCGGGTGTCTCCGGCATCTCCGG‑TTATATCCTTGCTGTACTGGCGATGACACCAAAATGTGCTTACTTCGCTAACATCGCGGGTTTGTGTGCGGCGAGGGCTGTCTCCGTCGTTGTCTCTGCTATTTTGCTGAAAACCGGCa < 2:53717/249‑1 (MQ=255) gTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGGCTGGGTTCTCCGGCATCTCCGG‑TTTTATCCTTGGTTTACTGGCGATGACACCCAAAGGGGCTTACTTCGCTAACATCGCGGGTGTGGGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGc > 1:443257/1‑250 (MQ=255) aTCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCTCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGTGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATGTCCTG‑TTGTATCCTTGCGGTACTTGCTATGACATCAAAAGGTGCTTGCGGCGCTAACATCGCGGGTGTGTGGGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTAGTTTGCTGAAAACCAGCaa < 1:98402/250‑1 (MQ=255) tCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTATGGTTTCTTCGGCCTCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGCTGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGcca > 1:516120/1‑247 (MQ=255) ccACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAACCCAGCAAAGt < 1:119893/251‑1 (MQ=255) aCGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGa < 1:324170/251‑1 (MQ=255) gAAATCTACTTCCCGTATGCGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTATGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGCTGACACCAAAAGGGGCTTACTTCGCTAAACTCGCGGGGGTGGGTGCGGCGATGGCAGTCTCCTTCGTTGTCTATGCTATTTTGCTGTAAACCAGCAAAGTGaa > 2:399655/1‑250 (MQ=255) aaaTCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACCATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCACAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGaa > 1:598853/1‑249 (MQ=255) aTCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAaaga > 1:407247/1‑250 (MQ=255) cTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCCAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGCAAACCAGCAAAGTGAcaga > 2:192835/1‑248 (MQ=255) cTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGc < 1:449584/237‑1 (MQ=255) cTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGGGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAaagacg > 2:490320/1‑248 (MQ=255) cTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGGGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGc > 2:449584/1‑237 (MQ=255) tACTTACCGTCTGTGCTGATGCATCCGCGTCTCATCCTCGCAGTCATCCCAGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGCCCGCGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAACAGGTGCTTACTTCCCTAACATCGCGGGGGTGTGGGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAAACAGAACAGTGAaagaaga > 2:592275/1‑250 (MQ=255) ttCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGGCACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCACAGTGAaagaaga > 1:385178/1‑247 (MQ=255) ccGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAgatgat < 1:24429/251‑1 (MQ=255) ccGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCATGGGCGGTGGTCTGGGTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTGCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAgatgat < 1:612993/251‑1 (MQ=255) aTGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATAttt > 2:456842/1‑250 (MQ=255) gtgCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAATAAGATGATATTGaa < 1:153532/251‑1 (MQ=255) gCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAagc > 1:41531/1‑251 (MQ=255) gCTGATGAATCCGCGTCTGATCCTAGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTTACTATCCTGGGCGGAGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGGTGTCTCTGCTATTTTGCTGAAAACCCGCAAAGTGAAAGAAGAAGATGATATTGGagc > 2:55269/1‑251 (MQ=255) tgatgaATCCGCGTCTGCTCCTCGCAGTCCTCCTCGGCGGTATGTCTGGCGTGTTCACGCTGACCATCCTGGGCGGTCGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGGGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTTTTTTGCTGAAAAcaa > 2:428242/1‑216 (MQ=255) tgatgaATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCGCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCa < 1:428242/218‑1 (MQ=255) gatgaATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCTATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAagca < 1:567428/249‑1 (MQ=255) gaATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAAAAGATGATATTGAAGCAGCAAc > 1:278344/1‑251 (MQ=255) gaATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGGGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGAGGAAATTGAAGCAGCaa > 2:23834/1‑250 (MQ=255) gaATCCGAGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAAc < 1:304691/251‑1 (MQ=255) aaTCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTTGTTTCGCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACt < 1:621927/251‑1 (MQ=255) tGATCCTCGCATTCATCCTCGGCGGAATGACTGGCGTGTTTACGCTGACTTTCCTGGGCGGGGGTCTGGTTTCTCCGGCATCTCCGGGTT‑TATCCGTGCTGTACTGGCGATGACACCAAAAGGTGCTTAATTCGCTAACATCCAGGGTGTGTGTGCGGGGAGAGCTGACTCCTTCGTTGGCTCTGCTATTTTGCTGAAAACCCGCAAAGTGAAAGAAGAAGATGCTATTGGAGCAGCAAATCGTCGTaggc > 2:293392/1‑248 (MQ=255) tGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCCAAGTGAAagaagaaga > 1:14788/1‑222 (MQ=255) tGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAagaagaaga < 2:14788/222‑1 (MQ=255) gATCCTCGCAGTCATCCTCGGCGGTATGACTGGCTTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGc < 1:606539/250‑1 (MQ=255) gATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTa < 1:384988/247‑1 (MQ=255) gATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTa > 2:384988/1‑247 (MQ=255) gATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTAGTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTAt < 2:216253/248‑1 (MQ=255) aTCCTCGCAGGCATCCGCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTGCGCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTGCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACGCGTCGTATGCAg < 2:455057/251‑1 (MQ=255) ccTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGGGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTAATGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGGTGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGCAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAgg > 1:66428/1‑250 (MQ=255) cTCGCAGTCATCATAGGCGGTATGACTGGCGTGTTCAAGCTGACTATCCTGGGCCGGGGTCTGGTTTCTCCGGCATCTCCGGATT‑TATACTTGCTGTACTGGCGATGACACCAAAAGGTGCTTAATTCGCTAAAATAGCGGGGGTGTTTGCGGCGATGGCTGTCTCCTTCGTTATCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCCACTCGTCGTATGCAggcc > 2:593454/1‑249 (MQ=255) cGCAGTCAGCCTCGGCGGTATGCCTGGCGTGGTCACGCTGACTATCCTGGGCTGTGGCCTGGTTGCCCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTGTGTGCTATTTGGCTGAAACCCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACAt < 1:256096/251‑1 (MQ=255) cAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCCGCAAAGTGAAAGAAGAAGATGATATTGAag > 1:518137/1‑224 (MQ=255) cAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAag < 2:518137/224‑1 (MQ=255) cAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGa < 2:179518/251‑1 (MQ=255) gTCATACTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGCGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGGGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCGGCAAAGTGAAAGAAGAAGCTGATATTGAAGCAGCAACtcttccgatgcgggaaatgaa > 2:115336/1‑231 (MQ=255) gTCAGCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGaaa < 2:587533/251‑1 (MQ=255) tCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGaa > 1:570745/1‑249 (MQ=255) cATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGc < 2:242514/251‑1 (MQ=255) ccTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATTACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTg < 1:420424/250‑1 (MQ=255) cggcggTATTACTTGCGTGGTCACTCGGACTATCCTGGGCGGTGGTCTGGGTTCTCCGGCATCTCCGG‑TTTTAGCCTTGCTGGACTGGCGATGACAACAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGGGGGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGTTAGTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGAGATGGAAGCAGCAACTCGTCGTATGCAAGACATGAAAGCTGAGt < 2:608468/250‑1 (MQ=255) ggTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTaaa < 2:35718/251‑1 (MQ=255) aTGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAgg < 2:624380/250‑1 (MQ=255) gACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGgcgc > 2:475620/1‑251 (MQ=255) gtgcACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGTGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCt < 1:537357/248‑1 (MQ=255) gtTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGCGTCTAAAGGCGCATCTCCGCt > 2:18218/1‑251 (MQ=255) cACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCCGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTc > 2:499103/1‑251 (MQ=255) aCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTct > 1:471039/1‑251 (MQ=255) aCTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACGGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCGCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGAGATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGc < 1:245777/251‑1 (MQ=255) aTCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCg < 1:615045/249‑1 (MQ=255) tCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGc > 2:604043/1‑247 (MQ=255) tGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGAtgtg > 1:87538/1‑250 (MQ=255) gtggtCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACg > 1:386502/1‑251 (MQ=255) tCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCt > 2:484378/1‑251 (MQ=255) gTTTCTCCGGCATCTCCGG‑TTTTATACTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGc > 2:428019/1‑251 (MQ=255) gTTTCTCCGGCATCGCCGG‑TTTTATCCGTGCTGTAGTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTGCGTTGTCTCTGCTATGTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTTAAGCAGCAACTCGTCGGATGCAGGTCATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTGTGCTTGCGATGTGACTAACTGCCTGAGc < 2:328044/251‑1 (MQ=255) tttCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGcc > 1:40287/1‑251 (MQ=255) tcccGGTATCTCCGG‑TTTTATCCGTGCTGTACTGGCGATGACAACAAAAGGTGCTTACTTCGCTATCAGCGCGGGTGTGTGTGCGGCGATGGCCGTCGCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGAGGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAGGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCac < 2:341226/248‑1 (MQ=255) cGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCATCTTTTTCTCTGCTATTGGGCTTAAAACCAGCAAAGTGAAAGAAGAAGATTATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCAGCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCacgtac < 1:268343/251‑1 (MQ=255) gCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCacgtacgt < 1:109214/251‑1 (MQ=255) | TGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACGT > NC_000913/3772931‑3773414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |