Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,719,429	G→A	intergenic (‑385/‑49)	yiaF ← / → yiaG	DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,719,429	0	G	A	100.0%	180.6 / NA	57	intergenic (‑385/‑49)	yiaF/yiaG	DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/26);  total (31/26)

TGTTTCATTGTTAAAAAATGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATG  >  NC_000913/3719192‑3719674                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      tgttTCATTGTTAAAAAATGATAGTGCCGTTGCCTTTTTTGTAGCCAGATATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCa                                                                                                                                                                                                                                           <  1:27423/250‑1 (MQ=255) ggattcaatgatAAAAAATGCTAGTGCCTTTGCATTTTTTCTAGACAGAGAATTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATAGGCATTTATCAGATCGTTAGGTGGCTATAAGTAACGCTAAATGACCGGCTGAATCGAGTCATAGCCCGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACAATTCTTAATAAGCTTCGATGCa                                                                                                                                                                                                                                           >  2:461866/12‑250 (MQ=255)         tGTTAAAAAATGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGCAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCAAGCTAAATGCCAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTATGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACg                                                                                                                                                                                                                                    >  1:607335/1‑249 (MQ=255)             aaaaaaTGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCg                                                                                                                                                                                                                              <  2:485553/251‑1 (MQ=255)             aaaaaaTGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCg                                                                                                                                                                                                                              <  1:217057/251‑1 (MQ=255)               aaaaTGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTACTAAGCTTCGATGCACTTCACGATCCCGc                                                                                                                                                                                                                             >  2:446652/1‑250 (MQ=255)                 aaTGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAgt                                                                                                                                                                                                                          >  1:135316/1‑251 (MQ=255)                   tGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCCCGATCCCGCAgtgt                                                                                                                                                                                                                        >  2:582949/1‑251 (MQ=255)                     aTAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGa                                                                                                                                                                                                                      <  1:467010/251‑1 (MQ=255)                           ccgttttcTTTGTTGTAGCCATAGATTTACGCCTTCGCAGAAGGTTAGATGTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTAGCATATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCAGGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGGCTATTCTTAATAAGCTTCGATGCAATGCACGTTCCCGCAGTGTTATTTgag                                                                                                                                                                                                                <  2:369624/244‑1 (MQ=255)                             gTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCCCGATCCCGCAGTGTGATTTgagg                                                                                                                                                                                                               >  2:352131/1‑250 (MQ=255)                               ttccTTTTTTGTAGCCAGAGATTTACGCCTACGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTgaggag                                                                                                                                                                                                             <  1:85500/248‑1 (MQ=255)                                 cctttaatGTACCCAGAGACTTACGCTTTAGCAGGCGGATAGATTTTAGAAATCTTTACAAAACAGGAGTGTTAAGCGATATATAGGAATTTATCATATCGTTAGGTGGATATAAGTCACGCTCAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACACCGCTGTGTCTGCTTTTCCCGACTATTCTTCATAAGCGTCGATGCACTTCACGATACCGCAGCGAGTTTTGAGGAGttt                                                                                                                                                                                                          >  2:254397/8‑251 (MQ=255)                                          tAGCCAGAGATTTACGCCTTCGCGGAAGGTTAGATTTTCGAACTCTTTACAAAACAGGCGTGGTAAGGGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGa                                                                                                                                                                                                  >  2:61272/1‑250 (MQ=255)                                              cAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGTCTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAAtata                                                                                                                                                                                             <  1:508635/251‑1 (MQ=255)                                                                 aGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCTATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCt                                                                                                                                                                          <  1:562834/251‑1 (MQ=255)                                                                 aGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCAAGCTAAATGAAAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCt                                                                                                                                                                          >  1:367943/1‑251 (MQ=255)                                                                  gAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTg                                                                                                                                                                         <  1:198285/251‑1 (MQ=255)                                                                   aaGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGt                                                                                                                                                                        <  2:412419/251‑1 (MQ=255)                                                                      gTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTg                                                                                                                                                                      >  1:39760/1‑250 (MQ=255)                                                                        tAGATTTTCGAACTCTTTACAAAACAGGAGTGGGAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGAGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTg                                                                                                                                                                      >  2:280230/1‑248 (MQ=255)                                                                        tAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGagc                                                                                                                                                                   >  2:190647/1‑251 (MQ=255)                                                                         aGATTTTCTAAATCTTTGCAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGTCTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCGGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGagc                                                                                                                                                                   <  1:16265/250‑1 (MQ=255)                                                                          gATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTGGagcag                                                                                                                                                                 <  2:72566/251‑1 (MQ=255)                                                                             tttCGAAATCTTTACAAACCAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCt                                                                                                                                                              >  2:59264/1‑251 (MQ=255)                                                                                 gAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGCATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGa                                                                                                                                                           >  1:305935/1‑250 (MQ=255)                                                                                            aaaaCAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGtttt                                                                                                                                               <  1:595861/251‑1 (MQ=255)                                                                                               aCAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTaa                                                                                                                                            <  1:446652/251‑1 (MQ=255)                                                                                                cagaaGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTaa                                                                                                                                            >  2:369378/5‑250 (MQ=255)                                                                                                      tGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTAGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTCAAGATGa                                                                                                                                      >  1:407227/1‑250 (MQ=255)                                                                                                              gaTATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGa                                                                                                                              >  2:306395/1‑250 (MQ=255)                                                                                                                      gtcATTTATCAGATCGTTAGGTGTCTATAAGTCACGCTAAATGACCGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCAGTGTCTGCTTTTCCCGACTATTCTTAATATGCTTCGATGCAATTCACGACCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATACAATGCATGAGCTGTTGAGCAGCCTGGAACAGAGTGTTTTGAAAGATGAAACGCAGAAAATTAccc                                                                                                                     <  2:606698/249‑1 (MQ=255)                                                                                                                            tATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTGTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACg                                                                                                                <  1:415180/250‑1 (MQ=255)                                                                                                                              tCAGATCGTTAGGTGGCTATAAGACACGCTAAATGACAGGCTGAATAGAATCATAGCCAGAGCATGCCCTGACTTAACCCAGATGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGcac                                                                                                             >  1:100314/1‑251 (MQ=255)                                                                                                                                     gTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGaaca                                                                                                       >  1:588355/1‑250 (MQ=255)                                                                                                                                               tataAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTAc                                                                                            >  1:220271/1‑251 (MQ=255)                                                                                                                                                   aGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGaa                                                                                        <  1:432176/251‑1 (MQ=255)                                                                                                                                                        cGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTg                                                                                    >  2:168194/1‑250 (MQ=255)                                                                                                                                                                gACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCACTGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGACATTGAACAGTTAc                                                                           >  1:316127/1‑251 (MQ=255)                                                                                                                                                                      cTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCATGTACCGAAATTGAGCAGTTACGaaa                                                                       >  1:404199/1‑249 (MQ=255)                                                                                                                                                                        gaatcgaatcATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAggg                                                                   <  1:538576/251‑1 (MQ=255)                                                                                                                                                                        gaatcgaatcATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAggg                                                                   >  1:541431/1‑251 (MQ=255)                                                                                                                                                                              aataaTAGCCAGAGCATGCCTTCACTTCACCCCGCTGTCTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGAGTGATTTGAGGAGTTTTCAATGGCCTATAAAGATCCAATGCAAGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAACGATGAACCGCAGAAAATTACCCCGACGCCCAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGCCAgg                                                              >  2:425900/5‑250 (MQ=255)                                                                                                                                                                                       cAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATc                                                    <  2:234636/251‑1 (MQ=255)                                                                                                                                                                                                cccTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATGAAAAATCGATGATTTc                                           <  2:367943/251‑1 (MQ=255)                                                                                                                                                                                                cccTGACTGCTCCCCTCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCATCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTc                                           <  1:265983/251‑1 (MQ=255)                                                                                                                                                                                                    gACTTCACCCCGCTGTTTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTTTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGcc                                        <  1:582949/250‑1 (MQ=255)                                                                                                                                                                                                     aCTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCg                                      >  1:187750/1‑251 (MQ=255)                                                                                                                                                                                                        tCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCgg                                     <  1:168194/249‑1 (MQ=255)                                                                                                                                                                                                            cccGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGACTATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTAAGAAAAGGGACAGGCTTAAAAATCGATGATTTCGCCCGGGTTTTg                               >  1:294646/1‑251 (MQ=255)                                                                                                                                                                                                               gCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGc                            <  1:302642/251‑1 (MQ=255)                                                                                                                                                                                                                cTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCg                           >  1:173500/1‑251 (MQ=255)                                                                                                                                                                                                                              cccGACTCTTCTTAATAAGCTTAGATGCCATTCACGATCCCGACGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCACTGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGAAGAAAATTACCCTGCCGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTCTCGGTCGCCATg             >  2:539610/1‑251 (MQ=255)                                                                                                                                                                                                                               ccGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATgg            <  1:437919/251‑1 (MQ=255)                                                                                                                                                                                                                                cGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCCGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACAGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGt           >  2:190767/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTaaa        <  2:33916/249‑1 (MQ=255)                                                                                                                                                                                                                                         ttAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATg  >  2:189726/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      TGTTTCATTGTTAAAAAATGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATG  >  NC_000913/3719192‑3719674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑