Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,665,576	C→T	V79I (GTT→ATT)	gadX ←	DNA‑binding transcriptional dual regulator GadX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,665,576	0	C	T	97.7%	135.7 / NA	44	V79I (GTT→ATT)	gadX	DNA‑binding transcriptional dual regulator GadX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/15);  minor base G (0/1);  total (28/16)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GTCCACTCATGGGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAACC‑AGG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGAC  >  NC_000913/3665332‑3665817                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        gtCCACTCATGGGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATtttttt                                                                                                                                                                                                                                                   >  1:147797/1‑251 (MQ=255)   ccACTCATGGGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTGCAGTGATGAACTAGTAAGGAAGAAATTTTTTTa                                                                                                                                                                                                                                                  <  2:524111/250‑1 (MQ=255)        cATGGGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTGTTAtgtg                                                                                                                                                                                                                                              <  2:321465/249‑1 (MQ=255)                  attattattGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTa                                                                                                                                                                                                                                  <  1:169208/251‑1 (MQ=255)                     atttatgagaaCCGTACAAACTCGTGTTCGCAGTTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTTTTTCACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGAGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGTAATATCTCTACGACAGTGATGAACGAGTAATGAAGAAAGTTTTGTAGGTGCTTGTAGCTTTAAt                                                                                                                                                                                                                                <  2:305059/241‑1 (MQ=255)                            aTAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCAGGAAGATTTAATGcctcctc                                                                                                                                                                                                                        <  1:321579/251‑1 (MQ=255)                               aCCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCtt                                                                                                                                                                                                                     <  1:252773/251‑1 (MQ=255)                                       aaCTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCg                                                                                                                                                                                                             >  1:166822/1‑251 (MQ=255)                                            gtgtTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCggg                                                                                                                                                                                                           >  1:233499/1‑248 (MQ=255)                                              gttccaaTGTTCGGATGTAAAACGTTCAGCAGCCGCGGTATAAAGTGCTCTTCCTCAAGAAACACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTCTGTGCATGAAGATTTAATGCCTCCTCCTTGAACATCGGgataa                                                                                                                                                                                                       >  2:113660/7‑247 (MQ=255)                                                tCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAaa‑t                                                                                                                                                                                                    >  1:540235/1‑250 (MQ=255)                                                   cATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTCTAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CGGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATg                                                                                                                                                                                                >  2:110654/1‑251 (MQ=255)                                                     tGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATAGGGAGAAA‑TAATGa                                                                                                                                                                                               >  2:519953/1‑250 (MQ=255)                                                                             gcagcGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGGGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACa                                                                                                                                                                      <  1:387290/251‑1 (MQ=255)                                                                                 cGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCGTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAAt                                                                                                                                                                  <  1:255809/251‑1 (MQ=255)                                                                                  ggTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAAGACAAAAtt                                                                                                                                                                 <  2:507831/251‑1 (MQ=255)                                                                                      taAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGTACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGCTTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACACAATTTTc                                                                                                                                                              >  1:250375/1‑250 (MQ=255)                                                                                       aaaGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCa                                                                                                                                                             >  1:221251/1‑250 (MQ=255)                                                                                               cATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACtt                                                                                                                                                   >  1:211110/1‑250 (MQ=255)                                                                                               cATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACtt                                                                                                                                                   >  1:123414/1‑250 (MQ=255)                                                                                                     cAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACt                                                                                                                                            >  1:208639/1‑251 (MQ=255)                                                                                                      aaGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACTTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTc                                                                                                                                           <  1:250886/251‑1 (MQ=255)                                                                                                           aaaCAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAAtt                                                                                                                                      >  1:41960/1‑251 (MQ=255)                                                                                                                  taacaccacaaagaggaaTGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGAGCAACTTACTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGACATTTTTTTATGTGCATGAAGATTTAATGACTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGACTACCACATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTg                                                                                                                              >  2:54167/17‑251 (MQ=255)                                                                                                                   aacaacGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGCAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGc                                                                                                                             >  2:84709/1‑251 (MQ=255)                                                                                                                       acGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATcc                                                                                                                         >  1:99400/1‑251 (MQ=255)                                                                                                                               aTTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAAc                                                                                                                 <  1:76575/251‑1 (MQ=255)                                                                                                                                  aaTGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAACA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATGTTTTTATGTACATGAGGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAAcc                                                                                                                >  1:250490/1‑249 (MQ=255)                                                                                                                                  aaTGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAACC‑a                                                                                                              <  2:532769/250‑1 (MQ=255)                                                                                                                                          tttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAACC‑AGG‑TCACCg                                                                                                     <  1:176275/250‑1 (MQ=255)                                                                                                                                             aCGTAGCATTTCTGCATAGCCACGATTCTTATTCTGCGATA‑GTTGAGCAACTTACTGAACAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTACTGAAGCAATTTTTTTATGTGCATGAAGATTTAATGCATCCTCCTTGAGCCTCGGGAGCAT‑TAATGAAAGCGTGCCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGC‑CATGCCTAAGCAAAAACC‑AGG‑TCCCCGcaat                                                                                                 >  2:39773/1‑248 (MQ=255)                                                                                                                                                 aGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATATACTCGAATTTGG‑CTTGCATCCGCAAAAACC‑AGG‑TAACCGCCATTaa                                                                                              >  1:146017/1‑250 (MQ=255)                                                                                                                                                              aGCGCAGATTCTTCTTCTGCGATC‑GTTGCGCAACTTCCTGACACA‑CAGGAATATCTCTACTCCAGTGATGAACGAGTAATGAAGCCATTTTTTTATGTGCATGAAGATTTACTGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACCCGAATACAAACTTTTCAA‑CAC‑CCTTCTCTACTCGACTTTGG‑CTTGCATCCGCAAAAACC‑AGG‑TCACCGCCATTCACATAGCGATATTc                                                                                 >  2:610186/1‑250 (MQ=255)                                                                                                                                                                     aTTCTTTTTCTGC‑ATAAGTTGCGCAACTACCTAAAAAC‑CAGAAATATCTCTACTATAGTGATAAAGGAGTAAGGAAAAATTTTTTTTATGTGCATGAAGTTTAAATCCCTCCCCCTTGAGCATGGGGAAAAA‑TAATGAAGGCTGGTCCCTTGACACGAATAAAAAATTTTCAA‑CTC‑TCTTATCT‑CCCCAATTTGGGTTTGCA‑CCCCAAAAACCCAGG‑TCACCGCCATTAAAATAGCGATATTCACCATTaa                                                                         <  1:248159/251‑1 (MQ=255)                                                                                                                                                                        cTTATTCTGCGCTA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATCTCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAACC‑AGG‑TCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                       >  1:483280/1‑250 (MQ=255)                                                                                                                                                                                       gTTGCGTAACTTCCCGAAAAA‑CAGGAATATCTCTAATACAGTGATGCCCGAGTAATGAAGAACTTTTTTTCAGTGCATGAAGATTTACTGCCTAATCATTGCGCATCGGGAGAAC‑TAATAAAAGCGTGTCACTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCACAAACC‑AGG‑TCACCGCCATTAAAATAGCGATATTAACCATTAACCATGGTGAGCatatat                                                        >  2:257711/1‑251 (MQ=255)                                                                                                                                                                                              aaattCCTGAAAAAACGGAAATTTCTCAACTAAAGGGATGAAGGAGTAATAAAGAAATTTTTTTATGTGCATGAAAATTAAATCCCCCCTCCTGGAGCATGGGAAAAAA‑TAATAAAAGCGTGTCCCTTGACACAAATA‑AAAATTTTCAAACCC‑TCTTATCTCCTAAATTTTGG‑CTTGCACCCGAAAAACCC‑AGG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                  <  1:110654/248‑1 (MQ=255)                                                                                                                                                                                                    ataaaaaa‑CAGAAATATCTTTACTACAGTTAGAAACGATTAAGAAAGAAATTTTTTTATTTGAATGAAGTTTTATTCCCTCCTCCTTGAGCA‑CGGGAAAAAATAAAAAAGGCTCGCCCCTTGACACGAATACAAA‑TTTTAAA‑CCCCACTTACCTCTTCAATTTTTG‑CTTGCA‑CCCAAAAAACC‑GGGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCAt                                           <  2:166822/248‑1 (MQ=255)                                                                                                                                                                                                       aaaaa‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAACC‑AGG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                        >  1:383296/1‑251 (MQ=255)                                                                                                                                                                                                                         tactacAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAACC‑AGG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                        >  1:152036/1‑250 (MQ=255)                                                                                                                                                                                                                         tactacAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTACATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTCCTCGAATTTGG‑CTTGCATCCGCAAAAACC‑AGG‑TCACCGCCATTACAATAGCGATATTCACCATTAACAATGGTGAGAATATATTTATGt                                                  >  1:547443/1‑224 (MQ=255)                                                                                                                                                                                                                                        cGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTACTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCAGCAAAAACC‑AGG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt         >  1:581556/1‑250 (MQ=255)                                                                                                                                                                                                                                         gAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACAAGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAACC‑AGG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt         >  1:601485/1‑249 (MQ=255)                                                                                                                                                                                                                                              aTGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAACC‑AGG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGAc  >  1:227327/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        GTCCACTCATGGGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATA‑GTTGCGCAACTTCCTGAAAAA‑CAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAA‑TAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAA‑CAC‑ACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAACC‑AGG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGAC  >  NC_000913/3665332‑3665817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑