Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,518,721	T→C	Q115R (CAG→CGG)	aroK ←	shikimate kinase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,518,721	0	T	C	95.8%	205.1 / ‑2.2	71	Q115R (CAG→CGG)	aroK	shikimate kinase 1
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (36/32);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CGACATCCACCTTAATTACTGTACCCGCAGACGAGTGTATATAAAGCCAGAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCTGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGTTCGTTTCTCAATCTCTTGATCGGAAT  >  NC_000913/3518491‑3518964                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    cgACATCCACCTTAATTACTGTACCCGCAGACGAGTGTATATAAAGACAGAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCttt                                                                                                                                                                                                                                 >  1:543202/1‑251 (MQ=255) cgACATCCACCTTAATTACTGTACCCGCAGACGAGCGTCTATCAAGCCAGACTTCGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCttt                                                                                                                                                                                                                                 >  2:77292/1‑251 (MQ=255)          ccTTAATTTCTGTACACGCAGACGAGTGTATATAAAGCCATAAGTAGTTGCTTTCCATCATGTGAATAATCTGGGTTGCAACCTCTTTAGCTCTTTGATCATCAGTGCGAATCGTCTCGTCGGCAATCTCTTCATACAGCGGATTTCGTTCATTGGCCAACGCTTCCAGAACTTCACTCCGCGG‑TTTTTCAACGTGCATCAACGGGCGTTTCTTATCACTCTGCGTGCGGACAATTTGCGTTTCGATgttc                                                                                                                                                                                                                        <  1:461847/251‑4 (MQ=255)                        cccGCAGACGAGTGTATATAAAGCCAGAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATaaa                                                                                                                                                                                                           >  1:66645/1‑250 (MQ=255)                        cccGCAGACGAGTGTATATAAAGCCAGAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATGGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTACGGTGGTCGTTGCAAGATAAAc                                                                                                                                                                                                          <  2:543202/251‑1 (MQ=255)                            cAGACGAGTGTATATAAAGCCAGAATTAGTGGCTTTCCAGCATGGGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGAc                                                                                                                                                                                                       >  2:535644/1‑250 (MQ=255)                                cGAGTGTATATAAAGCCAGAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGc                                                                                                                                                                                                  >  1:4654/1‑251 (MQ=255)                                  aGTGTATATAAAGCCAGAATTAGTTGCTTGCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCa                                                                                                                                                                                                <  1:8091/251‑1 (MQ=255)                                    tgtATATAAAGCCAGAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGAcaacgcaacg                                                                                                                                                                                              >  2:560767/1‑251 (MQ=255)                                       atataAAGCCAGAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTATCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTCCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCGGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATTGTCGTTTCAAGATAAACGACAACGCCACGAg                                                                                                                                                                                            <  1:24411/250‑1 (MQ=255)                                       atataAAGCCAGAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAg                                                                                                                                                                                            <  2:21487/250‑1 (MQ=255)                                       atataAAGCCAGAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGc                                                                                                                                                                                           <  1:222904/251‑1 (MQ=255)                                              gCCAGAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGaa                                                                                                                                                                                       >  1:32225/1‑248 (MQ=255)                                                  gAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGt                                                                                                                                                                                >  1:461354/1‑251 (MQ=255)                                                      tAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTAc                                                                                                                                                                             <  1:137208/250‑1 (MQ=255)                                                       aGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTAcgc                                                                                                                                                                           <  2:132819/251‑1 (MQ=255)                                                              ttCCAGCATGTGAATAATCTGGTT‑GCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCCTTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGGGCCGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGGTGGTCGTTTCAAGATAAACGACAACGCCACGGGCGGAAAGACGGGTACGCGGTTcccg                                                                                                                                                                   >  2:487122/1‑248 (MQ=255)                                                              ttCCAGCATGTGAATAAGCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCGCTTCATACAGCGGATTTCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTTCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTc                                                                                                                                                                      <  1:618146/249‑1 (MQ=255)                                                                ccAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAg                                                                                                                                                                                                                <  2:104355/205‑1 (MQ=255)                                                                ccAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAg                                                                                                                                                                                                                >  1:104355/1‑205 (MQ=255)                                                                   gCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGAt                                                                                                                                                               <  1:153230/251‑1 (MQ=255)                                                                   gCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGAt                                                                                                                                                               >  2:11756/1‑251 (MQ=255)                                                                         gAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTcaca                                                                                                                                                         <  1:614396/251‑1 (MQ=255)                                                                          aataatCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACCGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTcac                                                                                                                                                          >  1:590086/1‑249 (MQ=255)                                                                                      tGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCa                                                                                                                                             <  1:487122/250‑1 (MQ=255)                                                                                       gCAACCTCTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCGTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGAGGGTCGTTGCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTATGCGTTGCACGGGAGTTCACAGAGCCACCGCCAGt                                                                                                                                           <  2:598219/251‑1 (MQ=255)                                                                                       gCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGt                                                                                                                                           >  2:20170/1‑251 (MQ=255)                                                                                          aCCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCAACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCAACGAGCCGCCGCCAGTAGc                                                                                                                                        >  2:319804/1‑251 (MQ=255)                                                                                            cACTTTAGCGCTTTGATCATCAGTAAGAATGGTCACGTCGGAAATCTCTTCATACAGCGGATTGCGTTAATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGGTTCAAGATAAACGACAACGACACGAGCGGAAAGAGGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCACCAGTAGcc                                                                                                                                       >  2:358858/1‑250 (MQ=255)                                                                                                 gatcgcTTGGATCATCATTACGAATGGTCACTTCGGTAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCCCGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCTCGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGcac                                                                                                                                  <  2:589253/247‑1 (MQ=255)                                                                                                       tttGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATAccc                                                                                                                           >  1:30062/1‑251 (MQ=255)                                                                                                        ttGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGCGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCt                                                                                                                          >  2:225200/1‑251 (MQ=255)                                                                                                                caGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCg                                                                                                                  <  2:4654/251‑1 (MQ=255)                                                                                                                 aGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATGTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCgg                                                                                                                 <  1:266970/251‑1 (MQ=255)                                                                                                                     cgaatgttccccgcgtcAATCTCTTCATACATCGGATTGCGTGCATTGGCCAACGCTTCCAGAACTTCACGCGGCGGCTGTT‑CTACGTGCAGCCACGGGCGTTTTCTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACACAGCCACGAGCCGTAAGACGGATATGCGTGTTACGGGATTTCACTGGGCCGCCGCAAGGAGCCAGCACACGACCCTGTTtcgccgtacc                                                                                                             >  2:337895/17‑243 (MQ=255)                                                                                                                                   ggCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGACAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATTCCCTGTTTCTCGGTCAACTCATTGATGACCt                                                                                               >  2:227070/1‑251 (MQ=255)                                                                                                                                     aaaTCTCTTCATACAGCGAATTGCGTTAATTGGCCAACGCTTCCAGAAATTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGAGCAAGTTGCTTTTCGATGGTCGTTTCACGATAAACGACAACGCCACGCGCGGAAAGACGGTTACGCGTTTAACGGGATTTCACAGAGCCGCCGCACGTAGCCAGCACCATACCCCACTTCTCGGTCAACTCATTGATGCCCttt                                                                                             >  2:256642/2‑251 (MQ=255)                                                                                                                                      aaTCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCCGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCtttt                                                                                            >  1:329944/1‑251 (MQ=255)                                                                                                                                         ctctTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTttctt                                                                                         >  1:122287/1‑251 (MQ=255)                                                                                                                                            ttCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGCCAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTcgc                                                                                      >  2:349055/1‑251 (MQ=255)                                                                                                                                              cATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCTGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTcgcg                                                                                     <  1:402159/250‑1 (MQ=255)                                                                                                                                               aTACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCTCGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGAt                                                                                   <  1:21777/251‑1 (MQ=255)                                                                                                                                                     cGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCgg                                                                              >  2:216001/1‑250 (MQ=255)                                                                                                                                                     cGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATAACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCgg                                                                              >  1:380610/1‑250 (MQ=255)                                                                                                                                                           gCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGATCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGTAAGCctt                                                                       <  2:325920/251‑1 (MQ=255)                                                                                                                                                           gCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCctt                                                                       <  1:486721/251‑1 (MQ=255)                                                                                                                                                             gTTCATTGGCCAACGCTTCAAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGAGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCcttct                                                                     >  1:552604/1‑251 (MQ=255)                                                                                                                                                                cATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTCGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCttcttc                                                                   <  1:11756/250‑1 (MQ=255)                                                                                                                                                                   ttgCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGGGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGAGGGTCTTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCGTCTTCGcc                                                                <  2:380610/248‑1 (MQ=255)                                                                                                                                                                   gggCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGGGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTGCGCGATCGCGGAAGCCTTCTTCGCCt                                                               <  2:329944/250‑1 (MQ=255)                                                                                                                                                                      ccAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCt                                                            <  1:225200/251‑1 (MQ=255)                                                                                                                                                                        aaCGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTa                                                           >  2:72680/1‑250 (MQ=255)                                                                                                                                                                                   aaCTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAAccc                                               <  2:517822/251‑1 (MQ=255)                                                                                                                                                                                       tCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTGCAAGATAAACGACAACGCCCCGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCGGTTTCTCGGTCAACTCATTGATGACCTTTTCTGCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAAGCGAAAACCCAg                                             <  2:30062/249‑1 (MQ=255)                                                                                                                                                                                             ggcgg‑AGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCGAGATAAACGGCAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGGCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATc                                     >  2:42077/1‑251 (MQ=255)                                                                                                                                                                                                     tttCAACGTGCCGCACAGGGCGATTTTTATCACGCTGCGTGCGTGCACGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGCGCTGAAAGACGTTTACGCATTTCACGGGCTTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGTAAACCAAGCCCACATCCGCTCCg                              >  2:550354/1‑251 (MQ=255)                                                                                                                                                                                                     tttCAACGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCg                              >  1:253474/1‑251 (MQ=255)                                                                                                                                                                                                     tttCAACGTGCACCAACGGGCGTTTTTTATCACGCCGCGGGCGTGCAAGTGGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATCCCCTGTTTCGCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTcc                               <  2:324046/250‑1 (MQ=255)                                                                                                                                                                                                        aaaCGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGtt                           <  2:545587/250‑1 (MQ=255)                                                                                                                                                                                                         aaCGTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGAACGCCAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGACAGACGGTTACGCGATTAACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTACGCGATCGCGGAAGCCTTCTTCGCCTTCTCAATCGAAAACCCCGCCCACATCAGCTCCGGTTc                          >  2:568275/1‑251 (MQ=255)                                                                                                                                                                                                           cGTGCATCAACGGGCGTTTGTTAGCCCGCCGCGTGCGGGCCAGTTGCTTTTCGATTGTCGTTTCAAGATAAACGACAACGCCACGATCGGAAAGCCGGTTACGCGTTTCACGGGATTCCACAGTGCCGCCGCCAGTAGCCAGCACAAGACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGTTCGt                        <  1:561920/251‑1 (MQ=255)                                                                                                                                                                                                            gtgaagcaACGGGCGTTTTTTATCTCGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGCTAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGCGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTATTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGCAAACCCATCCCCCAtaacctacgcttcgtt                       >  2:66527/5‑236 (MQ=255)                                                                                                                                                                                                            gTGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCGCCGGTTCGtt                       <  1:149744/251‑1 (MQ=255)                                                                                                                                                                                                             tGCAGCAACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGTTCGttt                      >  1:459222/1‑251 (MQ=255)                                                                                                                                                                                                                agcaACGGGCGTTTTTTATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAAAGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGTTCGTTtctc                   >  1:102998/1‑251 (MQ=255)                                                                                                                                                                                                                            tttttATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGTTCGTTTCTCAATCTCTTGAt        <  1:602392/250‑1 (MQ=255)                                                                                                                                                                                                                             ttttATCCCGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGTTCGTTTCTCAATCTCTTGATc       <  1:20170/250‑1 (MQ=255)                                                                                                                                                                                                                              tttATCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGTTCGTTTCTCAATCTCTTGATCgg     >  2:130913/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTCACGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGTTCGTTTCTCAATCTCTTGATCGGAAt  >  1:130962/1‑251 (MQ=255)                                                                                                                                                                                                                                    aCGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACt                                                                                                           >  2:130358/1‑143 (MQ=255)                                                                                                                                                                                                                                    aCGCCGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACt                                                                                                           <  1:130358/143‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    CGACATCCACCTTAATTACTGTACCCGCAGACGAGTGTATATAAAGCCAGAATTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGG‑TGTTTCAACGTGCAGCAACGGGCGTTTTTTATCACGCTGCGTGCGTGCAAGTTGCTTTTCGATGGTCGTTTCAAGATAAACGACAACGCCACGAGCGGAAAGACGGTTACGCGTTTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGTTCGTTTCTCAATCTCTTGATCGGAAT  >  NC_000913/3518491‑3518964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑