Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,433,900	C→T	L71L (CTA→TTA)	def →	peptide deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,433,900	0	C	T	98.0%	153.4 / NA	50	L71L (CTA→TTA)	def	peptide deformylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (23/26);  minor base A (1/0);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGCTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTC  >  NC_000913/3433656‑3434132                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         tCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAgtgt                                                                                                                                                                                                                                    >  2:106734/1‑251 (MQ=255)    ttCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTa                                                                                                                                                                                                                                  <  1:298247/250‑1 (MQ=255)    ttCTAATTCCTGAACACATCTCTGGAGATTTATGGCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCGTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTa                                                                                                                                                                                                                                  <  2:552840/250‑1 (MQ=255)      cTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATAGTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCATCCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTa                                                                                                                                                                                                                                  <  2:172856/248‑1 (MQ=255)        aattCCTGAACCCATCTCTGGCGATTTATGTAAGTTTTGCAAGGGTTACATATTCAGGACGAGCGGCGTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGACGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAACCCGAGACGAACGTATAGTGTTaatca                                                                                                                                                                                                                              >  1:342041/4‑250 (MQ=255)        aattCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTAAATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTaatca                                                                                                                                                                                                                              >  1:191325/4‑250 (MQ=255)           tCCTGAATACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTACGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCCTCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATcc                                                                                                                                                                                                                          >  2:100329/1‑251 (MQ=255)            ccTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATcc                                                                                                                                                                                                                          >  1:68966/1‑250 (MQ=255)                 acacATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCt                                                                                                                                                                                                                    >  2:551589/1‑251 (MQ=255)                        tctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGa                                                                                                                                                                                                              >  1:277568/1‑250 (MQ=255)                                   atgtacgTTTTGCAAGTGTTACATATTCCGGACGAGCGGCGTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAAGGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGATCTTTTAGAAAAAAgcggcg                                                                                                                                                                                                   <  2:556297/244‑1 (MQ=255)                                       cAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTTTCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGACATCCATCAACGTATCATTGTTATTGATGTGTCGGAAAACCGTGACGAGCGGTTAGTGGTAATCAATCCAGAGCTTTTAGAATAAAGCGGCGAAACa                                                                                                                                                                                              <  2:536482/251‑1 (MQ=255)                                          ttttGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGc                                                                                                                                                                                           >  1:40726/1‑251 (MQ=255)                                               cAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTg                                                                                                                                                                                       >  2:521746/1‑250 (MQ=255)                                                aaGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTgaa                                                                                                                                                                                     <  1:525370/251‑1 (MQ=255)                                                    gtTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTgaagaa                                                                                                                                                                                  <  2:591598/250‑1 (MQ=255)                                                        cATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTg                                                                                                                                                                             >  2:549787/1‑251 (MQ=255)                                                                    gAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATccc                                                                                                                                                                 <  2:277568/251‑1 (MQ=255)                                                                     aGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCt                                                                                                                                                                <  1:318563/251‑1 (MQ=255)                                                                        ggCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGa                                                                                                                                                              >  2:216827/1‑250 (MQ=255)                                                                               cAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATGCATCGTATCGTCGATGATATGTGCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGGCGATCCCTGAACAACGTg                                                                                                                                                      <  2:64300/251‑1 (MQ=255)                                                                                     tGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTa                                                                                                                                                 <  1:354321/250‑1 (MQ=255)                                                                                     tGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTa                                                                                                                                                 <  1:311815/250‑1 (MQ=255)                                                                                        aaaaCCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGAGGATATGTTAGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCAGCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGc                                                                                                                                             <  2:168958/250‑1 (MQ=255)                                                                                         aaaCCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGcc                                                                                                                                            <  1:330299/251‑1 (MQ=255)                                                                                                 agaagaAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCgcgcgc                                                                                                                                      >  1:135352/1‑249 (MQ=255)                                                                                                       agTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGAAGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGCTATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATACCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGaa                                                                                                                                >  1:601211/1‑249 (MQ=255)                                                                                                        gTGAATGCAGACATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGACGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGATTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAAAAGGAATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGGGCTTTAGAGCCGCGCGCAGAGAAAGt                                                                                                                             >  2:61234/1‑251 (MQ=255)                                                                                                        gTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGCTGTACGCAGAAGAAGGTATCGGCATGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATAAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGt                                                                                                                             >  1:626434/1‑251 (MQ=255)                                                                                                           aaTGCAGACATTCAGCGTATCGTCGATGATATGTTCGAGAAGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCAAGAGCTTTTAGAAACAAGCGGCGAATCAGGCATTTACGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTa                                                                                                                           >  2:180013/1‑250 (MQ=255)                                                                                                            aTGCAGAAATTCAGCGGATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTaaa                                                                                                                         <  2:534033/251‑1 (MQ=255)                                                                                                              gCAGAAATTCAGCGTCTCGTCGATGATATGTTCGAGCCGATGTACGAAGAAGAAGGAATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGACGGTTGCCTGTCGATCCCTGACCAACGTGCTTTAGTGCCGCGCGCAGACAAAGTTaacat                                                                                                                       >  2:393186/1‑248 (MQ=255)                                                                                                                                      gatATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTa                                                                                                >  2:428062/1‑250 (MQ=255)                                                                                                                                         atGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATTAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTaaat                                                                                             <  2:107327/250‑2 (MQ=255)                                                                                                                                             tCGAGACGATGAACGCAGAAGAGGGTATTGGCCTGGCGGCAACCCAGGTTGATATCAAACAACGGATCATTGTTATTGATGTTTCGGAAAACCGTAACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAATAAGGGTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTACAATTCGCGCCCTTGACCGCGACgggacaccaatt                                                                                        >  2:452495/1‑241 (MQ=255)                                                                                                                                                       gTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGaa                                                                               <  1:521746/250‑1 (MQ=255)                                                                                                                                                                 gaagGTATTGGCCTGGCTGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTAGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGCCGGTTAACCATTTGAACTGGAAGCAGACGGTCt                                                                    >  2:219222/1‑251 (MQ=255)                                                                                                                                                                   agGTATTGGCCTGGCGGCCACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGt                                                                  >  1:488420/1‑251 (MQ=255)                                                                                                                                                                          ggCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCAt                                                           >  1:149788/1‑251 (MQ=255)                                                                                                                                                                           gCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATc                                                          <  1:78292/251‑1 (MQ=255)                                                                                                                                                                                       cccAGCTTGATATCCATCAACGTATCATTGTTCTTGCTGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCAt                                              >  2:454089/1‑251 (MQ=255)                                                                                                                                                                                            gTTGATTTCCATCAAGGTAACATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTTTCGATGCCTGAACAACGTGCTTTATTGCCGCGCGCAGAGAAAGTGAAAATGCGCGCCCTGGACCGTGACGGTAAACTATTTGAACTGGAAGCATACGGTCTGTTAGCCAGCTGTATTCAGCATGAGAt                                         <  2:74748/251‑1 (MQ=255)                                                                                                                                                                                               gATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGa                                      <  1:447545/251‑1 (MQ=255)                                                                                                                                                                                                          aCGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCg                           <  1:25368/251‑1 (MQ=255)                                                                                                                                                                                                                       aTTGATTTTTCGGAAAACCGTGACGACCGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGTTTGCCTGTCGATCCCTGAACAACGTTCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCATACGGTCTGTTAGCCATCTGTATTCAGTATGAGATGGATCACCTGTTCGGCAATCTGTTTAt              <  2:601211/251‑1 (MQ=255)                                                                                                                                                                                                                        ttGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCGCGGGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCTTCTGTATTCAGCATGAGATGGATCTCCTGGTCGGCAAACTGTTTATg             >  2:583677/1‑251 (MQ=255)                                                                                                                                                                                                                             gTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTa        <  1:509113/251‑1 (MQ=255)                                                                                                                                                                                                                              gttCGGAAAACCGTGGCGGACGGTTAGGGTTAAAAAAACCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTAt       <  2:234575/250‑1 (MQ=255)                                                                                                                                                                                                                                tCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATc      <  2:40726/250‑1 (MQ=255)                                                                                                                                                                                                                                   gAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTc  <  1:512967/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         TCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGCTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTC  >  NC_000913/3433656‑3434132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑