Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	100.0%	271.1 / NA	84	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/43);  total (41/43)

CTACAGTGCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCAGC  >  NC_000913/3329496‑3329989                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         ctACAGTGCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGAACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCAAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATg                                                                                                                                                                                                                                                       >  1:421394/1‑251 (MQ=255)          cccAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAAc                                                                                                                                                                                                                                              >  1:518279/1‑251 (MQ=255)             aaaGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAgg                                                                                                                                                                                                                                           >  2:197667/1‑251 (MQ=255)                 ccTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCggg                                                                                                                                                                                                                                       >  1:486558/1‑251 (MQ=255)                    ggTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTa                                                                                                                                                                                                                                     <  1:569691/250‑1 (MQ=255)                        atatGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAc‑c                                                                                                                                                                                                                               <  2:568856/251‑1 (MQ=255)                        atatGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGACGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGACATATGAGTACAAACAGGCGGGTATCAc‑c                                                                                                                                                                                                                               >  1:396822/1‑251 (MQ=255)                         tatGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAc‑cc                                                                                                                                                                                                                              >  1:175948/1‑250 (MQ=255)                          gtGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGTTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTg                                                                                                                                                                                                                             <  2:97994/250‑1 (MQ=255)                          atGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCggggtttccctg                                                                                                                                                                                                                              >  2:138396/1‑242 (MQ=255)                               ttttATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCC‑CTGGAGCg                                                                                                                                                                                                                        >  2:14259/1‑251 (MQ=255)                                 ttATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCgg                                                                                                                                                                                                                       <  1:270968/250‑1 (MQ=255)                                 ttATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CGGGAGCgg                                                                                                                                                                                                                       >  2:472905/1‑250 (MQ=255)                                  tataCGCGTGGCATCTTATTCCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCg                                                                                                                                                                                                                                         >  2:31161/1‑232 (MQ=255)                                  tataCGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCg                                                                                                                                                                                                                                         <  1:31161/232‑1 (MQ=255)                                        cgTGGCATCTTCTTACCCCGTTACGATGTTCAGCCAGGTCCGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTCCGCTGACGGGATGCCTGCCACACCGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAA‑CTGGATCGGAAAActg                                                                                                                                                                                                                >  2:561977/1‑250 (MQ=255)                                              aTCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGcc                                                                                                                                                                                                         >  1:205987/1‑251 (MQ=255)                                                 ttattaCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCAC‑CCGGAGCGGCACACTGCTGTGccca                                                                                                                                                                                                       >  2:404205/1‑248 (MQ=255)                                                    ttaCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAAAAATGCTGCGCCAGACTc                                                                                                                                                                                                   >  1:586759/1‑251 (MQ=255)                                                      aCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCTCTCCCGTTTTCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCa                                                                                                                                                                                                  <  1:321830/250‑1 (MQ=255)                                                           gTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGGGCGGAACACTGCTGCGCCAGACTCAGGtac                                                                                                                                                                                             >  2:495640/1‑248 (MQ=255)                                                                 aTGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCCATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACACCGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAAcc                                                                                                                                                                                      >  1:92251/1‑251 (MQ=255)                                                                       aGCCAGGTACGCCCCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACGGGAAGTGGTGCCAGGCGACCTGAACCGCTTTCCGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCATGTTACCGTACCTGGAAc                                                                                                                                                                                <  2:92492/251‑1 (MQ=255)                                                                        gCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACg                                                                                                                                                                               <  1:128058/251‑1 (MQ=255)                                                                          cAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGt                                                                                                                                                                             <  1:76296/251‑1 (MQ=255)                                                                               aCGCTCCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGtt                                                                                                                                                                         <  2:196653/250‑1 (MQ=255)                                                                               aCGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTg                                                                                                                                                                        <  1:549240/251‑1 (MQ=255)                                                                                cGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATAAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGGTCACGAAACTGGAACGGGAGTTGc                                                                                                                                                                       >  1:71460/1‑251 (MQ=255)                                                                                 gCACCCGCCCCCGTGGTTCCGCCGAGGCGCCCTACTGCGCGCTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTACCGTTGGCTTTTGCAGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGACATATGAGTTAAAAAAGGCGGGGATCAA‑CTGGAGAGGAAAACTCCAGGGGCAGACGCAGGCTAACGAACCAGGGACGGtgaatgcc                                                                                                                                                                      >  2:431243/1‑244 (MQ=255)                                                                                 ccACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGcc                                                                                                                                                                      <  2:285759/250‑1 (MQ=255)                                                                                   aCCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGGGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAg                                                                                                                                                                    <  1:437092/251‑1 (MQ=255)                                                                                   aCCCTCCCCCGTGGTTCTGCCGAAGCGCAAGACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACTGGATGCCTGCCACAGCGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTaaaa                                                                                                                                                                                                                                               <  1:603556/177‑1 (MQ=255)                                                                                   aCCCTCACCCGTGGTTATGCCAAAGCGCAATACTGCGCACTCGATGTGGTGCCAGGCGACCTGAACCGATTTACGCTGACGGGATGCCTGCCCCAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGCGTTaaaa                                                                                                                                                                                                                                               >  2:603556/1‑177 (MQ=255)                                                                                     ccTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTTCCAGTa                                                                                                                                                                  <  1:164198/251‑1 (MQ=255)                                                                                     ccTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGt                                                                                                                                                                   >  1:541718/1‑250 (MQ=255)                                                                                                    tGCCGAAGCGCCATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGGACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGGCGGCCCCGc                                                                                                                                                   >  1:83021/1‑251 (MQ=255)                                                                                                    tGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGTAAACAGTCGGCCCCGc                                                                                                                                                   >  1:590854/1‑251 (MQ=255)                                                                                                     gCCGAAGCGCAATACTGCGAACGGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCt                                                                                                                                                  <  2:477949/251‑1 (MQ=255)                                                                                                            cgcAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGAt                                                                                                                                           <  1:490631/251‑1 (MQ=255)                                                                                                              cAATACTGCGACCTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGACGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGAGGATCAC‑CTTGGGCGGGACCCTGCTGCACCAGACTCAGGATAACGAACCTGGAACGGTAGTTGCCAGGAGACAGGCGGCCCCGccgccccatct                                                                                                                                         >  2:544981/1‑241 (MQ=255)                                                                                                                  aCTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCtt                                                                                                                                     <  1:596217/251‑1 (MQ=255)                                                                                                                   cTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTCTCCC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGGAAACAGTAGGCCCCGCTGCACGATCTGCtt                                                                                                                                     >  1:83865/1‑250 (MQ=255)                                                                                                                     gCGAACTGGATGTGGTGCCAGGCGCCCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTaa                                                                                                                                   <  2:205987/250‑1 (MQ=255)                                                                                                                             gATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCt                                                                                                                          <  1:466821/251‑1 (MQ=255)                                                                                                                              aTGTGGTGCCAGGCGACCTGACCCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTg                                                                                                                         <  2:520758/251‑1 (MQ=255)                                                                                                                              aTGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGGAAACTGCTGCGCCCGACTCAGGGTAACGAAACTGGAGAGGTAGTTGCCAGTAAACAGTCGGGCCCGCCGCACCGTCTGCtttcgaatatgtct                                                                                                                         >  2:248613/1‑239 (MQ=255)                                                                                                                                  ggagCCAGGCGACATGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGAGCAGGATGGCGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCAC‑ATGGCGCGGAAAACTGCTGCGCCAGACTCAGGTTTACGAACCTGGGACGGGGGTTGCCAGTAAACAGTCGGCCCCGCCGCACGATCCGCTTAAGATTCTGCTGaaa                                                                                                                      >  2:121459/4‑250 (MQ=255)                                                                                                                                   gtgCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCAC‑CTGGAGCGGAACACTGCTTGGCCAGACTCAGGCTAACGAACCTGGAACAGAAGCTGCCAGTAAACAATCGGCCCCGCTGCACGATCTGCtttatatttttctgtaaa                                                                                                                     >  2:157299/1‑234 (MQ=255)                                                                                                                                       cAGGCGTCCTGAACCGCTTCACGTTGACGTGTTGCCTGCCTAAACGTTCTGAGCCGCTCCCGTTGGCTTTTGACGTGCAGGATGGAGCCAGCTATGCCGGTGCAAGTCTGAAATATGAGTTAAAACAGGCGGGTATCTC‑CTGGAGCGGAACACTGCGGCGCCAGACTCAGGTTAACGAACCGGGAACGGTAGGTGCCAGTAAACAGTCGGCTCCGCTGCACGATCTGCTTAAGATTATGCTGATAAAGTCg                                                                                                                <  2:408430/251‑1 (MQ=255)                                                                                                                                        aGGCGACCTTAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCgg                                                                                                               <  1:295778/251‑1 (MQ=255)                                                                                                                                        aGGCGACCTGACCCGCTTTACGCTGACTGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGGGCCAGCTATGCCGGTGCAATTCGGAAATATGAGTGAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGGCGGCCCCGCTGCACGATCTGCTTAAGATTATGCGGAAAAAGTCgg                                                                                                               <  2:565471/251‑1 (MQ=255)                                                                                                                                        aGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCC‑CTGGAGCGGGACACTGCTGCGCCAGACTCCGGTTAACGAACCTGGGACGGTAGTTTCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTTAAAAAGTCgg                                                                                                               >  2:139599/1‑251 (MQ=255)                                                                                                                                         ggCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCCGACTCAGGGTAACGAACCTGGAACGGTAGGTGCCAGTAAACAGTCGGCCCCGCCGCCCGATCTGCTTAAGATTATGCTGAAAAAGTCggg                                                                                                              >  1:156588/1‑250 (MQ=255)                                                                                                                                            gACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGaca                                                                                                            >  1:594889/1‑250 (MQ=255)                                                                                                                                             aCCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGacaac                                                                                                          <  1:269082/251‑1 (MQ=255)                                                                                                                                                 gAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATg                                                                                                       >  1:166721/1‑250 (MQ=255)                                                                                                                                                    ccGCTTTATGCTGACGGGAGGCCTGCCACAACGTTCTGCGCCGCTCCCGTTGGCTTTTGCCGTGCAGTATTGAGCCAGCTAGGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGATCGGAACACTGCTGCGCCATACTCATGTTAACTACCCTTGTACTGTAGTTGCCAGTAAACAGTCGGCCCCTCTTCACTATCGGCTTAAGATTATGCTGAAAAAGTCGTACAACATGATCg                                                                                                   <  2:546704/251‑1 (MQ=255)                                                                                                                                                               tGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAAACTCAGGGTAAAGCACCTGGAACGGGAGTTGCCAGTAAAAAGTCGGCCCCGCTGCACGATCAGCTTAAGATTATGCTTAAAAAGGCGGGCAACATGaggaccggtacgggt                                                                                        >  2:1566/1‑237 (MQ=255)                                                                                                                                                                   gggATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCa                                                                                                                                               >  1:506081/1‑192 (MQ=255)                                                                                                                                                                   gggATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCa                                                                                                                                               <  2:506081/192‑1 (MQ=255)                                                                                                                                                                    ggATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAAAGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGCCAACATGCTCGCCGATACGGTTTTCCg                                                                                   >  1:398979/1‑251 (MQ=255)                                                                                                                                                                         cctgccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATg                                                                               >  1:128036/1‑250 (MQ=255)                                                                                                                                                                         cctgccACAACGTTCTGAGCCGCTCCCGGTGGCTTTTGCCGTGCAGGATGGGGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGACCGCCGATACGGTTTTCCGCATGa                                                                              <  2:533531/251‑1 (MQ=255)                                                                                                                                                                             ccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAgg                                                                          <  1:119994/251‑1 (MQ=255)                                                                                                                                                                              cacaACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGc                                                                         <  2:156588/251‑1 (MQ=255)                                                                                                                                                                               acaACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGGTATGCCGGTGCAAGTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGAGCGCCGATACGGTTTTCCGCATGATAGGcc                                                                        <  2:609038/251‑1 (MQ=255)                                                                                                                                                                                 aaCGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCGGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCAt                                                                      <  1:627139/251‑1 (MQ=255)                                                                                                                                                                                         gAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCtt                                                              <  1:36236/251‑1 (MQ=255)                                                                                                                                                                                               cTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGGAACGGTTTTCCGCATGATAGGCCATGCCCGCTTCAAttt                                                        >  1:518609/1‑249 (MQ=255)                                                                                                                                                                                                 cccGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAAtgtg                                                       <  2:92251/250‑1 (MQ=255)                                                                                                                                                                                                   cGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCt                                                    <  1:30008/251‑1 (MQ=255)                                                                                                                                                                                                            ttGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTAT‑ACACTGGAGCGGGACACTGCTGCGCCCGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCCGTAAACCGTCGGCCCCGCTGCACGATCTTCTTAAGATTATGCTGAAAAAGGCGGACAACATGATCGCCGATACGGGTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATgg                                           >  1:377150/1‑251 (MQ=255)                                                                                                                                                                                                                 gTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑c                                     <  2:166721/251‑1 (MQ=255)                                                                                                                                                                                                                    cAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCCGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTTCTTCAGATTATGCTGAAAAAATCGGACAACCTGATCGCCGCTACGGGTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑cccg                                  >  1:372745/1‑249 (MQ=255)                                                                                                                                                                                                                    cAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCTAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACAGGGTCGCCGATACGGTTTGGCGCATGAGAGGTCATGCGCGGTTCAATGTGCCGGGAACATGGCGGG‑CCgg                                  <  2:557668/251‑1 (MQ=255)                                                                                                                                                                                                                     aGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCt                                                                                                                                                  <  1:250477/139‑1 (MQ=255)                                                                                                                                                                                                                               cAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCg                       <  1:196193/251‑1 (MQ=255)                                                                                                                                                                                                                                aGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGCACACTTCTGCGCCAGACTCAGGTTAACGAACCTGGAAAGGTAGTTGCCAGTAAACAGTCGGGCCCGCTGCCCGATCTGCTTAAGATTATGCTGAAAAAAACGGACAACATGATCGCCCGTCCGGTTTTCCGCATGATAAGCCATGCGCGCTTCAATGTGCCTGGGACATGGCGGGGCCgggcgggagccgg                       >  2:112207/1‑241 (MQ=255)                                                                                                                                                                                                                                  cTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGCGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTTAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGGTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGCACATGGCGGG‑CCGGGTCGGGCGCCgggg                    >  1:382105/1‑248 (MQ=255)                                                                                                                                                                                                                                  cTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGGACACTGCTGCGCCAGACTCAGGTTAACGGACCTGGAACGGGAGTTGCCAGTAAACCGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGGAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCCTGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGGCGGAAGCCgggg                    >  1:445059/1‑248 (MQ=255)                                                                                                                                                                                                                                      gCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTCa                <  2:173049/251‑1 (MQ=255)                                                                                                                                                                                                                                              aaTTCGGAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTCAGATCCTgc        <  2:486201/251‑1 (MQ=255)                                                                                                                                                                                                                                                    gAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCTCGCTGCACTATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCa    <  1:432323/249‑1 (MQ=255)                                                                                                                                                                                                                                                    gAAATATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCAGc  <  1:404205/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         CTACAGTGCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCAC‑CTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCAGC  >  NC_000913/3329496‑3329989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑