Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	867	C→T	S177S (AGC→AGT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	867	0	C	T	100.0%	120.8 / NA	41	S177S (AGC→AGT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/2);  total (39/2)

TGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATG  >  NC_000913/630‑1101                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           tGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTg                                                                                                                                                                                                                               >  1:123938/1‑251 (MQ=255)   cccAAATAACACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGAt                                                                                                                                                                                                                             >  1:501157/1‑251 (MQ=255)    ccAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGGCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCCAGTCGCATTCCGGCTGATc                                                                                                                                                                                                                            >  2:119341/1‑251 (MQ=255)          aaaaCATGTCCTGCATGGCATTCGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATgg                                                                                                                                                                                                                      >  2:114297/1‑251 (MQ=255)                     tGCATGGCATTCGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTg                                                                                                                                                                                                                    >  1:143126/1‑242 (MQ=255)                         tGGCGTTAGTTTGCTGGGGCAGGGCCCGGAGAGCATCAACGGTGCGCTGATTGGCCGTGGCGAGAAAATGTCGATCGCCATTATGGACGGCGTATTAGCAGCGCGCGGTCACAACGATACAGATATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGACTCTACCGTCGATATTGCTGAGTCTACCCGACGTATTGCGGCAAGTTGCATTCCGGCTGATCCCATGGTGATGATGGCAGGtt                                                                                                                                                                                                       >  2:582031/1‑251 (MQ=255)                                aGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGGGCTGATGGCAGGTTTCAccgc                                                                                                                                                                                                >  1:156689/1‑251 (MQ=255)                                                        aGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACtggg                                                                                                                                                                        >  1:565557/1‑250 (MQ=255)                                                         gCATCAACGCTGCGCTGCTTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACtggtg                                                                                                                                                                       >  2:581830/1‑251 (MQ=255)                                                                  ctgcgctgATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGAAGGTTTCACCGCCGGTAATGAAAAAGGAGAACTGGTGGTACTTgg                                                                                                                                                               >  1:288047/1‑250 (MQ=255)                                                                    gcgctgATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACg                                                                                                                                                            >  2:585065/1‑251 (MQ=255)                                                                       ctgATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGAAATGAAAAAGGCGAACTGGGGGTACTTGGACGCa                                                                                                                                                          >  1:12359/1‑250 (MQ=255)                                                                               ccGTTGCGAGAAAATGTCGCTCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCg                                                                                                                                                 >  2:462142/1‑251 (MQ=255)                                                                                  tGGCGAGAAAGTGTTGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGCTCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGCTCACATGGTGCTGCTGGCAGGTTTCACCGCCGGTAATGAAAAAGGAGAACTGGTGGTACTTGGACGCCACGGTTCCGact                                                                                                                                              >  2:97432/1‑251 (MQ=255)                                                                                               tCGATCGCCCTTCTGGCCGGCGTATTAGAAGCGCGCGTTCACAACGTTACCGTAATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAACAAGGCGACCTGGTGGTACTTGGCCGCAACGGTTCCGACTACTCCGcggcggg                                                                                                                                 >  2:323895/1‑250 (MQ=255)                                                                                               tCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGcggcggg                                                                                                                                 >  1:463793/1‑250 (MQ=255)                                                                                               tCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGt                                                                                                                                 >  2:198302/1‑251 (MQ=255)                                                                                                 gATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTgc                                                                                                                               >  1:129594/1‑251 (MQ=255)                                                                                                    cGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGact                                                                                                                                              >  2:359853/1‑233 (MQ=255)                                                                                                             ggCCGGCGTATTAGAAGCGCGCGGTCACAAAGTTACCGTTATCGATCCGGACGAAAAACTGCTGGCCGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGCCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGCTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGtt                                                                                                                   >  2:258859/1‑251 (MQ=255)                                                                                                               ccGGCGTGTTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGCATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGAATTCCGGCTGATCACATGGTGCTGATGGCCGGTTTCACAGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCGGCCAGtttc                                                                                                                 >  2:227933/1‑250 (MQ=255)                                                                                                                     tATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGcc                                                                                                           >  1:596700/1‑251 (MQ=255)                                                                                                                            aGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGCAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGAttgtt                                                                                                    >  2:263388/1‑251 (MQ=255)                                                                                                                              cgcgcgGTCACAACGTTACCGTTATCGATCAGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGCACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCCGGCTGTTTACGCGCCGATTGTTGc                                                                                                  >  1:198867/1‑251 (MQ=255)                                                                                                                                                  gTTATCGATCCTGTCGAAAAACTGCTGGCAGTGGGGCATTCCCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCCGGTTTCACCGACGGTAATGAAAAAGGCGAACTGGTGGCAATTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTCCGCGCAGATTGCTGCGAGATTTGGCCGGGCGTTgg                                                                              >  2:627216/1‑250 (MQ=255)                                                                                                                                                         aTCCGGTCGACAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTc                                                                                                                                                                                                     >  1:422665/1‑125 (MQ=255)                                                                                                                                                                cGAACAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCCCCGCCGGTAATGAAAAAGGCGAACTGGTGGCACTTGGACGCAACGGTTCCGACTAATCCGCGGCGGTGCTGGCTGGCTGTTTATGCGCCGGTTGTTGCGAGGTTTggacgggagttttcggggtctttcacc                                                                >  1:595954/1‑230 (MQ=255)                                                                                                                                                                  aaaaaCTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGACAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGGTGCCTGTTTACGCGCCCGTTGTTGCGAGGTTTGGACGGACGTTGACGGGGTCTCTACCTGc                                                              >  1:337812/1‑251 (MQ=255)                                                                                                                                                                        tgctgGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCcg                                                        >  1:381568/1‑251 (MQ=255)                                                                                                                                                                         gctgGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCcgc                                                       >  1:439628/1‑251 (MQ=255)                                                                                                                                                                                   gggCATTACCTCGAATCTACCGTCGATATTGCTGCGTCCACCCGCCGAATTGCGGCAAGTCGCATTCCGGCAGATAGCCAGGCACTGATGGCAGGTTTCACCGCCGGTACTGAAAAAGGCGTACTGGGGGTACTTGGACGCACCGGTTCCGACTACTCCGCGGCGGTGCTGGCAGCCTGTTTAAGCGCCGATTGATGAGAGATTTGGACGGACGTTGAACGGGTCtataacgtcggcccgggacatggacc                                             >  1:425323/1‑229 (MQ=255)                                                                                                                                                                                     gCATTACCTCGAATCTACCGTCGATATTGCTGAGTACACCCGCCGAATTGCGGCAAGTCGCATTCCGGCTGATCAAATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGAGGCGGTGCTGGATGCCTGTTTACGCGCCGATTGTTGCGGGCTTTGGACGGACGTTGACGGGGCCTATACCCTCGACCCGCGTCAGGTGGCCg                                           >  1:262244/1‑251 (MQ=255)                                                                                                                                                                                                    caccctctttttgggtatctccccccGCCGTATTGCTGCAAGTCGCATTCCGGCGTATCACATGGTGCTGATGGCAGTTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGa                            <  1:143448/228‑1 (MQ=255)                                                                                                                                                                                                       cGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCCGCCTGTTTTCGCGCCGGTTGTTGCGAGATTTGGACGGACGTTGACGGGGGCTATACCTGCGGCCCGCGGCAGGGGCCCGATGCGGGGttgttggagg                         >  2:542522/1‑247 (MQ=255)                                                                                                                                                                                                           gATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGACGGTAATGAAAAAGGCGAACTGGTGGTCCTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCCGCGACCCGCGTCAGGAGCCCGATGCGAGGGTGTTGAAggcggt                     >  1:412174/1‑246 (MQ=255)                                                                                                                                                                                                                       tCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGGTGCCTGTTTAAGCGCCGATTGTTGCGAGATTTGGACGGGCGGTGACGGGGTCTATACCTGCGAACCGCGTCAGGGGCCCGATGCGAGGTTGTTGGAGTCGGTGTCCTAcccgg          >  2:446314/1‑247 (MQ=255)                                                                                                                                                                                                                       tCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGAGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGCCTATACCTGCGCCCCCCGTCAGGTGCCCGGTGCGAGGGTGTTGAAGGCGATGGCCTACCAggg         >  2:345777/1‑250 (MQ=255)                                                                                                                                                                                                                        ccACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGa         >  1:401480/1‑250 (MQ=255)                                                                                                                                                                                                                            ccgccgTATTGCGGCAAGTCGAATCCCGGCGGTTCACATGGTGCTGAGGGCAGTTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGa    <  1:585065/251‑1 (MQ=255)                                                                                                                                                                                                                             cgccgTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCCGGTGCCCGATGCGCGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGAt   >  1:60219/1‑251 (MQ=255)                                                                                                                                                                                                                              gccgTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGGGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGCGGTTGTTGAAGTCGGTGTCCTACCAGGAAGCggtg  >  2:353802/1‑248 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           TGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATG  >  NC_000913/630‑1101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑