BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,176,344	G→A	H164Y (CAT→TAT)	cpdA ←	cAMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,176,344	0	G	A	98.2%	348.4 / NA	112	H164Y (CAT→TAT)	cpdA	cAMP phosphodiesterase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (51/59); minor base C (1/1); total (52/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

AGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATGCAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAAC  >  NC_000913/3176097‑3176586

agagTACGCCAGCCAGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACTCAGACTGTGTTGATCGATCCAACTACATCCCGCAGGTAGCTGATGATGATacag                                                                                                                                                                                                                                                 <  2:72577/251‑1 (MQ=255)

        ccAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATacagcagcagca                                                                                                                                                                                                                                         <  1:29710/251‑1 (MQ=255)

        ccAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATacagcagcagca                                                                                                                                                                                                                                         <  1:75340/251‑1 (MQ=255)

          aGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAAc                                                                                                                                                                                                                                       <  1:371634/251‑1 (MQ=255)

           gCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACg                                                                                                                                                                                                                                      <  1:340696/251‑1 (MQ=255)

            ccGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGt                                                                                                                                                                                                                                     >  1:262831/1‑251 (MQ=255)

              gggCTCGATGGGAGCCAGCTTAAAGTTGGAACAGTGCGGCTTCAACGGCATTCAGGTCGACTTCGCTGCCAGGAGTCCTCGACCATTCCATTCGAGATCCTGCTCCTGATTAATATGACTGCCCAGCAAGTATTTGACGTTCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCTGCGTTACTCAGACAGTGTTGAGCTAGCAAACTATAGCCCGCAGGTGGCGGATGAATATACAGCAGCTGCGACGTAt                                                                                                                                                                                                                                   <  2:381823/251‑1 (MQ=255)

              gggCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTAt                                                                                                                                                                                                                                   >  1:443888/1‑251 (MQ=255)

                 cgcgATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTGCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGc                                                                                                                                                                                                                                <  1:359076/251‑1 (MQ=255)

                    gATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGCGATCCAGCTCCTGCTGAATATGACCGCACAGCACGTATTTGACGTGCGGAAACTTCGCCAGCACGGAATCCCGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGCTGATGATACAGCCGCAGCAACGCATGGCGtt                                                                                                                                                                                                                             >  1:64109/1‑251 (MQ=255)

                      tGGTAACCAGCGTAAAGTTGGAACAGAGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCt                                                                                                                                                                                                                           >  1:203027/1‑251 (MQ=255)

                          agccAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATgtcttgctggc                                                                                                                                                                                                                        <  2:48466/248‑11 (MQ=255)

                          aTCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGgc                                                                                                                                                                                                                        <  2:459095/250‑1 (MQ=255)

                              aGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATc                                                                                                                                                                                                                   <  1:59367/251‑1 (MQ=255)

                                cGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCgg                                                                                                                                                                                                                 <  2:135022/251‑1 (MQ=255)

                                 gTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGc                                                                                                                                                                                                                >  1:262030/1‑251 (MQ=255)

                                 gTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCACAAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCAGCAGGAAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGc                                                                                                                                                                                                                >  1:444996/1‑251 (MQ=255)

                                      gTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGtt                                                                                                                                                                                                           >  2:53610/1‑251 (MQ=255)

                                      gTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGtt                                                                                                                                                                                                           <  2:152206/251‑1 (MQ=255)

                                                       tAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTcc                                                                                                                                                                                          >  2:446390/1‑250 (MQ=255)

                                                       tAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTc                                                                                                                                                                                           <  1:231918/250‑1 (MQ=255)

                                                       tAAACTGCAAACAGGACGAAGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGAATGTGTTGATCGAGCAAACTACAGCCCGCACGCAGCGGATGCTGATACAGTAGCAGCAACGTATGGCGTTCTGGCGCATCGGCAAGTTTACGtttaaacaactc                                                                                                                                                                                           >  2:112879/1‑240 (MQ=255)

                                                        aaaCTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCaa                                                                                                                                                                                         <  1:609022/251‑1 (MQ=255)

                                                         aaCTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCGTTCCAGGCTAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGTAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCATGTAGCGGATGATGATACATCAGCAGTAACGGATTGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAg                                                                                                                                                                                        <  2:303581/251‑1 (MQ=255)

                                                             gCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTAGGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCt                                                                                                                                                                                      <  2:307868/249‑1 (MQ=255)

                                                                  caGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTc                                                                                                                                                                               <  2:446666/251‑1 (MQ=255)

                                                                  caGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTc                                                                                                                                                                               <  1:507808/251‑1 (MQ=255)

                                                                   aGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCg                                                                                                                                                                              <  2:71777/251‑1 (MQ=255)

                                                                         aCGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCgctcagc                                                                                                                                                                        <  1:244255/251‑1 (MQ=255)

                                                                          gggCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCgctcagct                                                                                                                                                                       <  2:242525/250‑1 (MQ=255)

                                                                          cGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCgctcagcc                                                                                                                                                                       >  1:341076/1‑250 (MQ=255)

                                                                           ggCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGCTGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCgctcagctc                                                                                                                                                                      >  1:40490/1‑251 (MQ=255)

                                                                           ggCGGTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCgctcagctc                                                                                                                                                                      <  2:480255/251‑1 (MQ=255)

                                                                             cGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCAc                                                                                                                                                                    <  1:575994/251‑1 (MQ=255)

                                                                              gTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCAcc                                                                                                                                                                   <  2:600830/251‑1 (MQ=255)

                                                                                 gCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTg                                                                                                                                                                <  1:591770/251‑1 (MQ=255)

                                                                                      cagGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCTCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTTCGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCa                                                                                                                                                           <  1:203733/251‑1 (MQ=255)

                                                                                      aagGCGGTGCCCATTCCATGCGAGACCCAGCTCCTGAGGAATATGACCGCACAGCAAGTATTTTACGTGCGGAAACGTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTTCAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCATTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCa                                                                                                                                                           <  2:4353/250‑1 (MQ=255)

                                                                                             cgACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCaaa                                                                                                                                                    <  2:238894/251‑1 (MQ=255)

                                                                                                   ttCCAGTCGAGATCCAGCTCCTGATGAATATGCCCGCACAGCAAGTATTTGACGTGCGGCAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCCCGCCAAACACCTg                                                                                                                                              >  2:229793/1‑251 (MQ=255)

                                                                                                        gTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTAt                                                                                                                                         >  2:376852/1‑251 (MQ=255)

                                                                                                          cGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATcc                                                                                                                                       <  1:150267/251‑1 (MQ=255)

                                                                                                            agaTCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATcc                                                                                                                                       >  1:572277/1‑249 (MQ=255)

                                                                                                            agaTCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCCCACCGTGCGGCACGCCAACCACCTGGCTATcc                                                                                                                                       >  2:212226/1‑249 (MQ=255)

                                                                                                                  aGCTCCTGATGAATATGACCGCACAGCAAGAATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGCGCCAACTACAACCCGAAGGAAGCGGATGATGATACAGCAGCAGAAACGTATGGCGTTCTGGCGCATCGGCCGGTTTACGTTCCAGCCACTCAAGCTGCAAATCGCTCAGATCAACGTGCGGCACGACAAACACCTGGCAATcacgcaac                                                                                                                                 >  2:50054/1‑242 (MQ=255)

                                                                                                                    cTCCTGATGAATATGAACGCACAGCAAGTATTTGACGTGCGGACACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAgg                                                                                                                              >  2:240759/1‑250 (MQ=255)

                                                                                                                       cggatgaAAATGACCGCACAGAAATTTTTGGACTGGCGGAAACTTCGCCAGAACGTTACCCAGTTCGCCCGCGTTACGCAGACTGTGTTGACCGAGCCACCTACAACCCGCGGGTAGCGGATGATGATACAGCAGCAGCACCGTAGGGCGTCCTGGCGCATCGGCCAGTTTCCGTTCCAGCCACCCAAGCGGAAACTCGCTCAGCTCACCGTGCGGCACGCAAAACACCTG‑CTATCCAGCAACAGGATTTg                                                                                                                         <  2:42205/249‑1 (MQ=255)

                                                                                                                       cTGATGAATATGACCGCACAGCAAGTATTTGACGTGTGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGAttt                                                                                                                          >  1:613777/1‑251 (MQ=255)

                                                                                                                       cTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGAttt                                                                                                                          >  1:280727/1‑251 (MQ=255)

                                                                                                                         gatgaATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGc                                                                                                                        <  1:482512/251‑1 (MQ=255)

                                                                                                                         gatgaATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGc                                                                                                                        <  1:465598/251‑1 (MQ=255)

                                                                                                                         gatgaATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGAGGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACACGATTTg                                                                                                                         <  2:262030/250‑1 (MQ=255)

                                                                                                                             attatGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCAtt                                                                                                                    >  1:363578/3‑251 (MQ=255)

                                                                                                                              atatGACCGCGCAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTCCACCCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTg                                                                                                                   <  1:226847/251‑1 (MQ=255)

                                                                                                                               tatGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTCTCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGGTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGCTTTGCCATTGc                                                                                                                  >  1:605879/1‑251 (MQ=255)

                                                                                                                                 tGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTc                                                                                                                >  1:336837/1‑251 (MQ=255)

                                                                                                                                 tGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTc                                                                                                                >  2:190861/1‑251 (MQ=255)

                                                                                                                                   aCCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCAc                                                                                                              <  1:80594/251‑1 (MQ=255)

                                                                                                                                   aCCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCAc                                                                                                              >  1:557272/1‑251 (MQ=255)

                                                                                                                                      gCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGTCCAGTTTACGTTCCAGCCACGCAAGCTGAAACTCGCGCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAg                                                                                                                               <  2:302239/231‑1 (MQ=255)

                                                                                                                                               gTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACAcgc                                                                                                  >  2:156494/1‑251 (MQ=255)

                                                                                                                                                 aTTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACgcgc                                                                                                >  1:20118/1‑251 (MQ=255)

                                                                                                                                                  tttGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCt                                                                                               >  1:199493/1‑251 (MQ=255)

                                                                                                                                                   ttGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCt                                                                                               <  1:378627/250‑1 (MQ=255)

                                                                                                                                                    tGACGTGCGGAAACTGCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCtt                                                                                              <  2:557272/250‑1 (MQ=255)

                                                                                                                                                             gAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCgggg                                                                                      >  1:16691/1‑249 (MQ=255)

                                                                                                                                                             gAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGgag                                                                                    <  1:488213/251‑1 (MQ=255)

                                                                                                                                                              aaaCTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGgaga                                                                                   <  1:620411/251‑1 (MQ=255)

                                                                                                                                                                actccGCCACCACGGGATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGAGGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCAGTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATa                                                                                 <  2:40490/247‑1 (MQ=255)

                                                                                                                                                                 cTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATAc                                                                                >  2:539247/1‑251 (MQ=255)

                                                                                                                                                                  ttCGCCATCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACGTCAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTg                                                                                                                   <  1:615005/215‑1 (MQ=255)

                                                                                                                                                                  ttCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCa                                                                                                                                   >  2:480532/1‑199 (MQ=255)

                                                                                                                                                                  ttCGCCAGCACGGTATCAAGCTCGCCCGAGTTACGAAGACTGTGTTGATCGAGCCAACTAAAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGAATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCCCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTg                                                                                                                   >  2:615005/1‑215 (MQ=255)

                                                                                                                                                                   tCGCCAGCACGGTATCCAGGTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCATTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATAc                                                                                <  2:270956/249‑1 (MQ=255)

                                                                                                                                                                   gcGCCAGCACGGTTTCCAGTTCGCCCGCGTTACGCAGACTGTGATGATCGAGCCAACTACACCCCGCAGTGAGCAGATGATGATACAGCAGCAGAAACGTATGGCGTGCTGGCGCATCTGCCAGTGTACGTTCCAGCCACACAAGCGGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTAGCCAGCAACAGGAGTTGCCATTGCTCAACATTAAACACGCGCTTCTCCGTGGAGATAccc                                                                              <  2:586421/250‑1 (MQ=255)

                                                                                                                                                                     gCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCGTCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTTGCCGGGGAGATACCCGc                                                                            <  2:373131/251‑1 (MQ=255)

                                                                                                                                                                        aGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAACCACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATc                                                                         >  2:166914/1‑251 (MQ=255)

                                                                                                                                                                          cACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCt                                                                       <  1:307932/251‑1 (MQ=255)

                                                                                                                                                                            cGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGCTACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGt                                                                     >  1:261233/1‑251 (MQ=255)

                                                                                                                                                                             ggTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGt                                                                     >  1:460923/1‑250 (MQ=255)

                                                                                                                                                                                             gcgTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATcgc                                                    <  1:549448/251‑1 (MQ=255)

                                                                                                                                                                                                    gCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTgg                                             >  2:492078/1‑251 (MQ=255)

                                                                                                                                                                                                     cAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGa                                            <  1:298970/251‑1 (MQ=255)

                                                                                                                                                                                                      aGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGAACAAGCAGCAGCAACGAAAGGCGTTCTGGCGCAACGGCCAGCTTACGTTCCAGCCACCCAAGCTGAAACTAGCTCAGCTCACCGTGCGGCACAACAAACACCTGGCTATCCAGCAACCGGATTTGCCATTGCTCACCAATAAAAACGCGCTTTGCCGGGGAGATCCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGa                                            >  1:282998/1‑250 (MQ=255)

                                                                                                                                                                                                        aCTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAAt                                         <  1:53610/251‑1 (MQ=255)

                                                                                                                                                                                                          gatgtTGATCGAGCCAACTACATCCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCg                                       <  1:305293/249‑1 (MQ=255)

                                                                                                                                                                                                           gtgtTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCgg                                      >  2:367228/1‑250 (MQ=255)

                                                                                                                                                                                                           gtgtTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGt                                      <  2:280727/251‑1 (MQ=255)

                                                                                                                                                                                                               tGATCGCGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGtt                                  >  1:387409/1‑251 (MQ=255)

                                                                                                                                                                                                               tGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGtt                                  >  1:435452/1‑251 (MQ=255)

                                                                                                                                                                                                                       ccAACTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCa                          <  1:111462/251‑1 (MQ=255)

                                                                                                                                                                                                                         aaCTTCAACGCGCAGGTTGCGGGTGATGAGACATCAGCAGCAACGTATGTCGTTCTGGCGCATCGGCCATTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACATGTGGCTATCCATCAACAGGAGTTGGCATTGCTCCCCAATAAACACTCTCGTCGCCGGGGAGATACCCGTGTCTTGTAACGCGCTGTATATCGCTTGCTGGAAATCGTGGTTGCCCGGCAGc                        <  1:45595/251‑1 (MQ=255)

                                                                                                                                                                                                                         aaCTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGc                        <  1:257984/251‑1 (MQ=255)

                                                                                                                                                                                                                          aCTACAACCCGCAGGGAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCCCTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCCCGCCACACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGcc                       >  2:79005/1‑251 (MQ=255)

                                                                                                                                                                                                                           cTACAACCCGCAGGTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGcc                       >  1:489084/1‑250 (MQ=255)

                                                                                                                                                                                                                             aCAACCCGCAGGTAGCGGATGATGATCCAGCAGCAGCAACTGATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCa                      <  2:310321/249‑1 (MQ=255)

                                                                                                                                                                                                                             aCAACCCGCAGGTAGCGGATGAGGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCTATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGa                    <  1:329872/251‑1 (MQ=255)

                                                                                                                                                                                                                                        gTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGgcgcc         >  1:243507/1‑250 (MQ=255)

                                                                                                                                                                                                                                        gTAGCGGATGATGATACAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCa         <  1:367228/251‑1 (MQ=255)

                                                                                                                                                                                                                                               aggatgataCAGCTGCAGCAACGTAGGGCTTTCTGGCTCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAACCGCGCTCAGCTCACCGTGCGGCCCGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTCCATCGCGGGCTGGAAATCGTGGTTGCCCGTCAGCCAGACGCAGGGCTCATGAAAAc  <  2:483226/249‑1 (MQ=255)

AGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGAAACTTCGCCAGCACGGTATCCAGTTCGCCCGCGTTACGCAGACTGTGTTGATCGAGCCAACTACAACCCGCAGGTAGCGGATGATGATGCAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAAC  >  NC_000913/3176097‑3176586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`