Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,070,858	T→G	K129N (AAA→AAC)	fbaA ←	fructose‑bisphosphate aldolase class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,070,858	0	T	G	100.0%	311.4 / NA	94	K129N (AAA→AAC)	fbaA	fructose‑bisphosphate aldolase class II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (45/49);  total (45/49)

CAGTTCGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACTTTAGCAGCGGTTTCC  >  NC_000913/3070615‑3071104                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        cAGTTCGGTGTATGCGTAATCCACGTCTTACGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCAc                                                                                                                                                                                                                                                  >  1:512032/1‑251 (MQ=255)     gcGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCTCGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTAGTGAGAGCAGATGTCGATGTTCGCTTGCAGAGATTCTTCAGACAGGTCGATCGTGTGAGAAGAGAACAGCGGCTTATCGGTAGCTGCGAAGTGGTTTTCACCcg                                                                                                                                                                                                                                               <  2:361544/249‑1 (MQ=255)       ggagtATGCGTAATCAACGTCTTCCGGCTGGGTGCACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGt                                                                                                                                                                                                                                            >  2:199854/4‑251 (MQ=255)               cGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGa                                                                                                                                                                                                                                    <  1:383827/251‑1 (MQ=255)                  aaTCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCg                                                                                                                                                                                                                                 >  1:347545/1‑251 (MQ=255)                  aaTCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCg                                                                                                                                                                                                                                 >  2:173174/1‑251 (MQ=255)                  aaTCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCg                                                                                                                                                                                                                                 >  2:192156/1‑251 (MQ=255)                     cAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTTTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTGTTCACCCGCGTCCATCAGACCGTCg                                                                                                                                                                                                                              <  2:251301/251‑1 (MQ=255)                      aaCGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGACGTGGTTTTCACCCGCGTCCAACAGACCGTCg                                                                                                                                                                                                                              >  1:299415/1‑250 (MQ=255)                        cGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATc                                                                                                                                                                                                                           <  2:111987/251‑1 (MQ=255)                         gTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATcc                                                                                                                                                                                                                          <  1:38707/251‑1 (MQ=255)                          tCTTCCGGCGGGGTGTACAGTGCAGAAGCGGCCATGTGGCTGTGGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCTATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGTCAGGTTGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATc                                                                                                                                                                                                                           <  2:514261/249‑1 (MQ=255)                           cTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCAAGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCAAGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCAGCTTACCGGTAGCGGCGAAGGGGTTTTCACCCGCGTCCAACAGACCGTCGATCCAc                                                                                                                                                                                                                        >  1:299004/1‑251 (MQ=255)                                ggCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTATTCTTCACCACCGGTGCAACCCAGATCGATTTCCCGAGTCATGACGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCCCCAGCGTCCAACCGCCCGTCGATCCACGgc                                                                                                                                                                                                                     >  1:299313/1‑249 (MQ=255)                                  cTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGGTTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGgcagc                                                                                                                                                                                                                  >  2:16795/1‑250 (MQ=255)                                  cTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGgcagcc                                                                                                                                                                                                                 >  2:369398/1‑250 (MQ=255)                                    gggTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGgcagca                                                                                                                                                                                                                 <  1:473854/249‑1 (MQ=255)                                    gggTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGcagcag                                                                                                                                                                                                                <  1:368661/250‑1 (MQ=255)                                      gtgtACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCATCCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCGCCAGGTATTTAGAGCAGATGTCGATGTTCTCTTGCATAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCTGCTAACAGCCCGTCGATCCTCGTCAGCAGttt                                                                                                                                                                                                             <  2:609306/251‑1 (MQ=255)                                       tgtACCGTGGAGAAACGTGCAAGTGGCTGTAGTCCACGTCGTCTTCTTCACCACCGGTGCACCCCCGCTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGCATTTAGAGCAGTTTTCGATGTTCTCTTGCAGAGACTCTCCAGACAGGCCGCTCATGTGAGAAGAGAACCGCGGCTTACCGGTAGCTGCGCAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCCGTttc                                                                                                                                                                                                            >  2:570239/1‑251 (MQ=255)                                          aCAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTttcttc                                                                                                                                                                                                         <  2:192191/251‑1 (MQ=255)                                                    aGCGTCCATGTGGCTTTTGTCCTCGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCGGCGAAGTGGTTTTCACCCGCGGCTAACAGACCGTCGATCCACGGCAGCAGTTTCTTCTCGCTGTGGt                                                                                                                                                                                               <  2:620904/251‑1 (MQ=255)                                                              tgGCTGTTGTCCACGCCGTCTTCTTCACCACCGGCGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGGGGTCAGTGTGc                                                                                                                                                                                       >  2:458740/1‑249 (MQ=255)                                                               ggCTGTTGTCCACGCCGTCTTCTTCACCCCCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAgg                                                                                                                                                                                    >  2:542268/1‑251 (MQ=255)                                                               ggCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAgg                                                                                                                                                                                    >  1:353351/1‑251 (MQ=255)                                                                      gtCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGGTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAAcc                                                                                                                                                                              <  1:89879/250‑1 (MQ=255)                                                                         cACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTTGACATGCGCTCCAGGTATTTAGAGCAGCTTTCTATGTTCTCTTGCATAGATTCTTCAGACAGGTCTATCATGTGCGAAGAGAACAGCGTCTTACCGTTAGCTGCGAAGGGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGTAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGaa                                                                                                                                                                          <  2:214507/251‑1 (MQ=255)                                                                           cGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAac                                                                                                                                                                         >  1:241826/1‑250 (MQ=255)                                                                                     ttcACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGtt                                                                                                                                                              >  2:586895/1‑251 (MQ=255)                                                                                       caccaaCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCa                                                                                                                                                            <  1:1708/251‑1 (MQ=255)                                                                                         ccaccCGTGCACCCCAGCTCCATTTCCCGAGTTATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGAAGATTTCGATGTGCTCGTGCAGAGATTCTTCAGACAGGCCTATCATGTGATAAGAGAATGGCGGCTTACCGATATCTGCGAATTGGTTTTCACCCGCGTCCAACAGTCCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGGGGTCAGAGTGGAGGACAGCCGTAACTCCATCACGTTCAGc                                                                                                                                                          <  2:437066/251‑1 (MQ=255)                                                                                          caccGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAAGGTTCAGcc                                                                                                                                                         <  1:527075/251‑1 (MQ=255)                                                                                               gTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTg                                                                                                                                                    <  1:160695/251‑1 (MQ=255)                                                                                                tGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCTGTGTTCTCTTGCAGAGATTCTTCAGACACGTCGCTCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGCTCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGCGCAGGATAACCGGAACACCATAATGTTCAGCCATCTgg                                                                                                                                                   >  1:53150/1‑251 (MQ=255)                                                                                                 gCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTgg                                                                                                                                                   <  2:347545/250‑1 (MQ=255)                                                                                                   aaCCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGa                                                                                                                                                <  1:420182/251‑1 (MQ=255)                                                                                                           tCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGAt                                                                                                                                         <  1:380618/250‑1 (MQ=255)                                                                                                           tCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCGTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGAt                                                                                                                                         <  2:166731/250‑1 (MQ=255)                                                                                                            cGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATgc                                                                                                                                       >  1:415653/1‑251 (MQ=255)                                                                                                                   cAGAGTCATGCCGCTTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCAcc                                                                                                                                  >  2:563864/1‑249 (MQ=255)                                                                                                                      agTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCagcg                                                                                                                              >  2:224743/1‑248 (MQ=255)                                                                                                                         cATGCTGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGAta                                                                                                                           <  2:526026/250‑2 (MQ=255)                                                                                                                               gATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCa                                                                                                                    <  2:9981/251‑1 (MQ=255)                                                                                                                                    tGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTGCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGAt                                                                                                                <  2:470004/250‑1 (MQ=255)                                                                                                                                       aCATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATagc                                                                                                             >  2:294278/1‑250 (MQ=255)                                                                                                                                       aCATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATagc                                                                                                             >  1:570645/1‑250 (MQ=255)                                                                                                                                         aTGCGCGCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGGGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAAGGTGATGCGCACCAGAGATCGCGCCCAGGCTagcagc                                                                                                          >  2:141691/1‑251 (MQ=255)                                                                                                                                            cgcTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCAcc                                                                                                       <  1:173174/251‑1 (MQ=255)                                                                                                                                             gcTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCAccc                                                                                                      <  2:584892/251‑1 (MQ=255)                                                                                                                                                ccAGGTATTTAGAGCAGCTTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGc                                                                                                   >  1:179929/1‑251 (MQ=255)                                                                                                                                                ccAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGc                                                                                                   >  1:341348/1‑251 (MQ=255)                                                                                                                                                 catgTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCAGGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGAGCACCAGAGATCGCGCCCAGGATAGCATCACCCTGCg                                                                                                  <  2:444527/248‑1 (MQ=255)                                                                                                                                                  aGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGGCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCTCCCTGCgg                                                                                                 <  1:417928/251‑1 (MQ=255)                                                                                                                                                  aGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTGCGTCGCGCAGTGGGCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCgg                                                                                                 <  2:299415/251‑1 (MQ=255)                                                                                                                                                  aGGTATTGATAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTGTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGGTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCgg                                                                                                 <  2:481600/251‑1 (MQ=255)                                                                                                                                                    gTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGaa                                                                                               <  1:620545/251‑1 (MQ=255)                                                                                                                                                          agcgcataTTTCGATGTTCTATTGCAGAGATTCTTCAGACAGTTCTATCTGGTGAGAAGATATCAGCGGCTGCCCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGAGCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTTCAGGATAACCTGAACGCCAGAATATTCAGCCATCTGGTTAACGTGATGCGCACCAGAGTTCGCGGCCAGGATAGCAGCACCATGCGCAACGTCAg                                                                                         <  2:550835/244‑1 (MQ=255)                                                                                                                                                          agatcAGATTGCGATGTTCTCTTGCATCGATTCTTCAGACAGGTCGAGCATGTGAGAACAGAACAGCGGCTGACCTGGAGCTGCGAAGTGGTTTTCTCCCGCCTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCTCGCAGTGGTCAGTGTGCATGATAACCGGAACGCCCGAATTTTCAGCCATCGGCTGAGCGTGAGGTGCACCAGAGAGCGCCGCCAGGATagcagcccgcagtgtaccgtca                                                                                          <  2:81340/246‑17 (MQ=255)                                                                                                                                                                gATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCa                                                                                  <  1:211374/251‑1 (MQ=255)                                                                                                                                                                   ttCGATGTTCTCTTCCAGGGTTTCTTCAGACAGGACGATCATGTGAAAAGAGAACAGCGGCATACCGGGAGCTGCGACGTGGTTTTCACCCGCGTCCAACAGCCCGACGATCAACGGCAGCAGTTTCTTAGCGCAGCGGTCAGTGTGCAGGATAACCGGAACACCCTAATGTTCAGCCCTCTGGTGAACGTGATGCGCACCAGAGATCGCGGCGAGGATAGCACCACCCGGCGGAACGTC‑GACTTTCACGc                                                                               >  2:294543/1‑251 (MQ=255)                                                                                                                                                                        tGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATCGCAGCACCCTGCGCAACGTCAGA‑TTTCACGCCtttc                                                                          >  1:532741/1‑250 (MQ=255)                                                                                                                                                                         gTTCTCTTGAAGAGATTATTCAGCCAGGTAGATCATGTGAGAAGAGAACAGCGGCTTAACGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGCCCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGGTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTAc                                                                         >  2:54635/1‑251 (MQ=255)                                                                                                                                                                                tGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGAt                                                                  >  1:176122/1‑251 (MQ=255)                                                                                                                                                                                   agagATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCTCCCGCGTCCAACAGACCTGCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATCACCGGACCACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACTAGCGATaaa                                                               <  2:218374/251‑1 (MQ=255)                                                                                                                                                                                     agaTTCTTCAGACATGTCGATCATTTGAGAAGAGAACAGCGGCTTCCCGGTAGCTGCGATGTGGTTTTCACCCGCGTCCAACATACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGGTCAGCCATCTGGTGAACGTGATGCGCACCATAGAGCGCGCCCAGGATAGCAGCATCCTGCGGAACGTCAGC‑TTGCACGCCTTGACCAGCGATAAAg                                                              <  2:601029/250‑1 (MQ=255)                                                                                                                                                                                     agaTTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAAc                                                                                              >  2:23713/1‑219 (MQ=255)                                                                                                                                                                                     agaTTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAAc                                                                                              <  1:23713/219‑1 (MQ=255)                                                                                                                                                                                     agaTTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAgg                                                             >  2:385522/1‑251 (MQ=255)                                                                                                                                                                                       aTTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGaa                                                           <  1:471512/251‑1 (MQ=255)                                                                                                                                                                                                aCAGGTCGATCATGTGAGAAGCGAACAGCGGCTTCCCGGTAGCTGCGAAGTGGGTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTAACGCCTTTACCATCGGTAACGGAAGCACCACCg                                                  >  2:266068/1‑251 (MQ=255)                                                                                                                                                                                                      cGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGga                                             <  2:353351/250‑1 (MQ=255)                                                                                                                                                                                                        aTCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGaa                                          <  1:224743/251‑1 (MQ=255)                                                                                                                                                                                                        aTCATGGGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGgaga                                           >  2:111263/1‑250 (MQ=255)                                                                                                                                                                                                          cATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACt                                        >  1:188813/1‑251 (MQ=255)                                                                                                                                                                                                            tgtgAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATg                                                                                                                                                                >  2:151888/1‑130 (MQ=255)                                                                                                                                                                                                            tgtgAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATg                                                                                                                                                                <  1:151888/130‑1 (MQ=255)                                                                                                                                                                                                               gagaagagaaCAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGACCGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACg                                   >  1:250026/1‑251 (MQ=255)                                                                                                                                                                                                                agaagagaaCAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACg                                   >  1:315739/1‑250 (MQ=255)                                                                                                                                                                                                                 gaagagaaCAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGAt                                 <  1:105860/251‑1 (MQ=255)                                                                                                                                                                                                                    gagaaCAGCGGCTTAACGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCCGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAAc                              >  2:256510/1‑251 (MQ=255)                                                                                                                                                                                                                           gCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGAGCAGGCTAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCt                       >  2:372169/1‑251 (MQ=255)                                                                                                                                                                                                                            cGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCtt                      <  2:620552/251‑1 (MQ=255)                                                                                                                                                                                                                              gCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTa                    <  1:503087/251‑1 (MQ=255)                                                                                                                                                                                                                               cTTACCGGTAGCTGCGAAGTGGATTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTa                    >  1:434496/1‑250 (MQ=255)                                                                                                                                                                                                                                 tACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAAc                  <  1:626455/250‑1 (MQ=255)                                                                                                                                                                                                                                  aCCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACt                 <  1:118552/250‑1 (MQ=255)                                                                                                                                                                                                                                    cGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCTATCCACGGCAGCAGTTTCTTGGCGCAGTGGTTAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTTATGCGCGTCAGAGATCGCGTCCATGATAGAAGCGCCTTGCTGAACGTGATA‑TTTCACGCCTTTATCAGCGATAAAGGACGCACCAGCGTTGGAGAACTGAACGCTAACCTgcgttctatcttta              <  2:239170/251‑12 (MQ=255)                                                                                                                                                                                                                                    cGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACttt               >  1:123157/1‑250 (MQ=255)                                                                                                                                                                                                                                     ggTAGCTGAGAAGTGGTTTTCAACCGCGTCCAACAGACCGTCGATCAACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGCTAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACttt               >  1:186795/1‑249 (MQ=255)                                                                                                                                                                                                                                      gTAGCTGCGACGTGGTTTTCCCCCGCGTCCAACCGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACAGGAAAACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGCGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGACCGATAACCGGCGCTTTAACTTTagc            >  2:283638/1‑251 (MQ=255)                                                                                                                                                                                                                                            gCGAAGTGGTTTTCACCCGCGGCCAACAGACCGTCGAGCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCCTTAACTTTAGCAGCgg       <  2:129198/250‑1 (MQ=255)                                                                                                                                                                                                                                             cGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACTTTAGCAGCGGtt     >  1:31481/1‑251 (MQ=255)                                                                                                                                                                                                                                                aGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACTTTAGCAGCGGTTTcc  <  1:294278/251‑1 (MQ=255)                                                                                                                                                                                                                                                aGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAAAACCATAATGTTCAGACATCTGGTGAACATGATGCGCACCAGAGATCGCGACCAGGATAGCAGCACCCTGCGGAACGTACGA‑TTTCACGCCTTTACAAGCGATAAAGGAAGAACCACCGTTGGAGAACAGAACGATAACCGGCGCTTTAACTTTAGCAGCGGTTTcc  >  1:317665/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        CAGTTCGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGA‑TTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACTTTAGCAGCGGTTTCC  >  NC_000913/3070615‑3071104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑