Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,838,241	G→A	intergenic (‑136/+13)	hydN ← / ← ascG	putative electron transport protein HydN/DNA‑binding transcriptional repressor AscG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,241	0	G	A	98.4%	185.1 / NA	61	intergenic (‑136/+13)	hydN/ascG	putative electron transport protein HydN/DNA‑binding transcriptional repressor AscG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (25/35);  minor base C (1/0);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTC  >  NC_000913/2837996‑2838484                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ggggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATatgatg                                                                                                                                                                                                                                                  <  2:150103/251‑1 (MQ=255)           aTGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTa                                                                                                                                                                                                                                        <  1:371534/251‑1 (MQ=255)                 agcccTGATATTCGTGATGCGAAACCACGCACGCTACTTCGCAGGCAAGGCAACCAATACATTTACTCGCGTCAGCAATGCTGAAACGGTTAATCAAATTCTCCAGCAATGACAGTTAATGCGCAGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATcgcg                                                                                                                                                                                                                                   >  2:615790/4‑250 (MQ=255)                  gTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCACCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGaa                                                                                                                                                                                                                                 <  1:517073/251‑1 (MQ=255)                      tGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAAGATTCATCAAATTATCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAATCGATGTCATCGAAACTAGTGACGATGACATATGATGCCAATGTTTATCGCGCAgg                                                                                                                                                                                                                               >  1:259014/1‑249 (MQ=255)                       gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAg                                                                                                                                                                                                                             <  2:69907/250‑1 (MQ=255)                       gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGc                                                                                                                                                                                                                            <  1:56176/251‑1 (MQ=255)                           ttccgtatgaGAAACCACGCACGCTACTTCACATGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCTCGAAGGAGCAAt                                                                                                                                                                                                                         <  2:40447/244‑1 (MQ=255)                           ttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAAt                                                                                                                                                                                                                         <  1:606937/250‑1 (MQ=255)                                   gagaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTAGTTAGGGAAATTAATTTCTGTAATGCAGGAAAAAGTGTGTCAGCTACGGTAGGGACGATGACATATGATGACAAGGTTGATCGTGAAGGAGCAAGGGGTGAGTc                                                                                                                                                                                                                <  2:41631/251‑1 (MQ=255)                                   gagaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGAAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTc                                                                                                                                                                                                                <  2:127369/251‑1 (MQ=255)                                           acgcacgcTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGcggcgg                                                                                                                                                                                                         >  1:125368/1‑250 (MQ=255)                                             gcacgcTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCCGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATc                                                                                                                                                                                                      <  2:158779/251‑1 (MQ=255)                                                    aCTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTc                                                                                                                                                                                               >  1:183445/1‑251 (MQ=255)                                                      ttCACAGGCACGGCAACCAATACATTTACTCGCGTCCGCAATGATGACACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGCTACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTc‑cc                                                                                                                                                                                            >  2:358476/1‑250 (MQ=255)                                                          caGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTCCTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAA‑CGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGa                                                                                                                                                                                        >  1:84902/1‑250 (MQ=255)                                                            ggTACTGCAACCAATACATTTACTCGCGTCAGCAATGCTGAAACGGTTCATCAAATTCTCCATCAATGACAGTTAATGCGCCGATACATTCACAAATCATGACAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGATCAATGAGTGATTCGCGGCTTATCAGTTTTC‑GGCTGAAg                                                                                                                                                                                      <  2:28631/251‑1 (MQ=255)                                                             gTACGGCAACCGATACATTTGCTCGCGGCGGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGAGCCGATACAATCACAAATCATGCCAGTTTTAAATTTACTGTTATTTAAGGAAAATAATTTCTGTAATGCAGGAAAAACGATGTCATCGACTCTAGTGACGATGACATAGGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CACTGAAgg                                                                                                                                                                                     <  2:161984/251‑1 (MQ=255)                                                                     aCCAATACAGTTACTCGCGTCAGCAATGATGAAGCGGGTCAACAAATTCTCCAGCAATGACAGTGAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATATACTGTTATTTAAGGAAATTAAGTTCTGTAATGCAGGAAAAACGATGTCATCGACGCTAGTGACGATGACATATGAGGACAATGTTTAGCTCTAAGGAGCAATAAGTGAGTCGCGGCGGAGCAGTTTTC‑CGCTGAAGGTTGTcg                                                                                                                                                                               <  2:327710/249‑1 (MQ=255)                                                                        aaTACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGt                                                                                                                                                                          <  1:429864/251‑1 (MQ=255)                                                                                    cgcgTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCAGCGACACGAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTCTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCg                                                                                                                                                             <  2:540538/251‑1 (MQ=255)                                                                                             aaTGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTCTTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGGAGGTTTTCGGCGGTGAGAGA‑ACCCCGCCATCGAGc                                                                                                                                                    >  2:73702/1‑251 (MQ=255)                                                                                                gatgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCAt                                                                                                                                                  <  1:531437/250‑1 (MQ=255)                                                                                                 atgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCAGCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATaa                                                                                                                                                <  2:455556/251‑1 (MQ=255)                                                                                                       aggTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGTTACATTCACAAATCATGCCATTTTTTAAGTTACTGTTAATTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCTATGAGTGAGTCGCGTCGGATCAGTTTTC‑CGCTAAAGGTTTGCGGCGATGAGAAA‑TCCCCTCCATCGAGCATAAAAATCa                                                                                                                                          <  2:25108/250‑1 (MQ=255)                                                                                                                         aGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCt                                                                                                                        <  1:171220/251‑1 (MQ=255)                                                                                                                           cAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGa                                                                                                                      <  1:211061/251‑1 (MQ=255)                                                                                                                              tGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTAATCGACACTAGTGACGATGAAATATGATGACAATGTTTATcgc                                                                                                                                                                                                                                    >  2:355692/1‑140 (MQ=255)                                                                                                                               gACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑ACCCCGCCCTCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAata                                                                                                                   >  1:353679/1‑249 (MQ=255)                                                                                                                               gACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAAGGAGTGAGTCGCGGCGGATCAGTTGTC‑CGCTGAAGGTTTTCGGCTGAGAGAAA‑TCCCCGCCAGCGAGCATAAAAATCAGCCGCCCAAGAAGTGCCTGAatc                                                                                                                   <  2:280020/250‑1 (MQ=255)                                                                                                                                    ttAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGGGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCAtctc                                                                                                              >  2:206150/1‑250 (MQ=255)                                                                                                                                      aaTGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCGGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAg                                                                                                            <  1:604667/250‑1 (MQ=255)                                                                                                                                             cGATAAATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTCATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGCCAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGAACAGTTTTC‑CGCTGAAGGTTTTCGGAGGTGAGACG‑TTCCCGCCATCGAGCATAAAAATCAGCCGTACCATAATTTCATGAATCCTCTCCGTCACCgg                                                                                                     >  2:416909/1‑250 (MQ=255)                                                                                                                                              gATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGATACTAGTGACGCTTACATATGGTGACAAAGTTTATATCGATGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCTGGGTGA‑GGGTTTCGGCGGTGAGAAAATCCCGCCCAT‑AAGCATGAAAAACAACCGTCCAATAATTTCCttcatacactcaatcaacgat                                                                                                    >  2:518664/1‑230 (MQ=255)                                                                                                                                                  cATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAAtttt                                                                                               <  2:125368/251‑1 (MQ=255)                                                                                                                                                         aaaTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCCCCGGAATTTTTACGCTg                                                                                        >  2:167612/1‑251 (MQ=255)                                                                                                                                                            tCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGTGCATAAAAATCCGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTTCGCTgg                                                                                       >  1:412582/1‑249 (MQ=255)                                                                                                                                                                  cAGTTTTTAATTGACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAATGAGCAATGAGTGAGTCGCGGCGGATCATTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCAGCGAGCATAAAAATCAGCCGTCCAATAATGTCCTGAATCATCTCATTCACCGGAATTTTTACGCTGGAGAGCGcc                                                                               <  2:557925/251‑1 (MQ=255)                                                                                                                                                                     tttttAATTTACTGTTATTTAAGGAAATTACTTTCTGTAATGCAGGAAAAACGATGTCATCGAAACTAGTGACGCTGACATCTGATGACACTGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGGTTTCGGGGGTGGGACA‑TCCCCGCCATCGTTCATAAAAATTAGCCGTCCAATAATTTCCTGAATCATCTCCGGTACCGGAAATTTTACCCTGGAGAGCCCCgg                                                                             >  2:382296/1‑250 (MQ=255)                                                                                                                                                                       tttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATAAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGGGCGCCGGAAc                                                                          >  2:336534/1‑251 (MQ=255)                                                                                                                                                                          aaTTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACgg                                                                        <  1:336534/250‑1 (MQ=255)                                                                                                                                                                                   ttATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGAAGATGACATATGCTGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGATGAAGGTTTTCGGCGGAGAGAAA‑TCCCCGCCATCGCGCATAAAAATCAGTCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGacagggggagggggg                                                              >  2:562554/1‑237 (MQ=255)                                                                                                                                                                                       ttAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TACCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAAt                                                           >  1:391695/1‑250 (MQ=255)                                                                                                                                                                                             aaaTTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCaata                                                                                                                               >  1:227970/1‑176 (MQ=255)                                                                                                                                                                                             aaaTTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCaata                                                                                                                               <  2:227970/176‑1 (MQ=255)                                                                                                                                                                                              aattaattGCTGGAAGGCAGGAAAAACGATGTCATCGACACTAGTGACGATGAGATAGGATGACAATGTTTATCGCGAAGGAGCAATGAGGGAGACGCGGCGGATCAGTTTGC‑CGCTGAAGGTTTTCGGGGGTGAGAAA‑TCCCCGCCATCGAGCAGAAAAATCAGCGGTCCAATAATTTCCTGAATCATCGCAGTCACCGGAAGTTTTACGCTGGAGAGCGCCGGAACGGTGGAGGGGGCAAGAGCGatag                                                   <  2:553804/251‑2 (MQ=255)                                                                                                                                                                                                  aattTCTGTAATGCAGGCAAAACGATGTCATCGACACTAGTGACGCTGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGGTTTCGGCGGTGAGAAA‑TACCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGCATCCACTCAGTCACCGGAATTTTTTAGCTGGAAAACGCCGGCCCGGTGTAAGGGGCCATAGGGatctcctc                                               >  2:359729/1‑245 (MQ=255)                                                                                                                                                                                                  aattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATatcatc                                               <  2:624038/251‑1 (MQ=255)                                                                                                                                                                                                  aattTCTGTAACGCATGAAAAACTATGTCATTGACACTAGTCGCGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCCGCGGGTCATTTTTC‑CGTTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCAATCGAGCATAAAAATCAGCCGTCCAATAATTGCGTGAATCAGCTCAGTCACCGGAATTGGTACGCTGTAGATCGCCTGAACGGTGTAGGGGGCAATAGCGATatcat                                                <  1:357072/250‑1 (MQ=255)                                                                                                                                                                                                      tCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGaa                                            <  1:471640/250‑1 (MQ=255)                                                                                                                                                                                                       cTGTAATGCCGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGCTGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCCGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGCGCATAAAAATCAGCCGTCCCATAATTTTCTGCATCATCTCAGTCACCCGAATTTTTACGCTGGAAAGCGCCGGAACGGGGTAGGGGGGAATAGCGATATCCTCGAATc                                          >  2:420968/1‑251 (MQ=255)                                                                                                                                                                                                           aaTGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGa                                       <  1:599275/250‑1 (MQ=255)                                                                                                                                                                                                             tGCAGGAACAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATAAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGAGCCGGAACGGGGTAGGGGGCAATAGCGATATCATCGAATACGATa                                     >  1:225116/1‑250 (MQ=255)                                                                                                                                                                                                                     aaaCGATGTCATCGACACTAGTGACGATGACATATGATGACAATGGTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGAGAACTGACAcc                            <  1:124339/251‑1 (MQ=255)                                                                                                                                                                                                                          aTGTCATCGCCACTAGTGACGATGCCATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCCCCATCCCGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCAACTCAGTCCCCGGAATTTTTACGCTGGAAAACCCCGGAAAGGCGGAGGGGGCAATAGCGAACTCATCGAAACCGATAAATGACACCTGctc                       >  2:429857/1‑251 (MQ=255)                                                                                                                                                                                                                          aTGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGGGTAGGGGGCAATAGCGATATCATCGAATCCGATCACTGACACCTGctc                       >  1:543494/1‑251 (MQ=255)                                                                                                                                                                                                                            gTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTg                     <  1:118026/251‑1 (MQ=255)                                                                                                                                                                                                                                 cGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCAcc                <  2:166695/251‑1 (MQ=255)                                                                                                                                                                                                                                             cGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCCTAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGTGCCGGAACGGGGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGAAACCTGCTCTGGCACCGCTACGCCgcgc    >  1:395800/1‑251 (MQ=255)                                                                                                                                                                                                                                               aTGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTc  <  1:136976/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aCATATGATGACAATGTTTCTCGCGAAGGAGCAATGAGTGCGTCGCGGCGGATCAGTTTTC‑CGCTGACGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAAATTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTTGGGAGCGCCGGAACGGTGTAGGGGGCAAAAGCGAATTCATCGAATCCGATAACt                                  >  2:630069/1‑216 (MQ=255)                                                                                                                                                                                                                                                  aCATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACt                                  <  1:630069/216‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTC‑CGCTGAAGGTTTTCGGCGGTGAGAAA‑TCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTC  >  NC_000913/2837996‑2838484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑