Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,823,537	A→G	L78P (CTG→CCG)	recA ←	DNA recombination/repair protein RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,823,537	0	A	G	100.0%	193.4 / NA	66	L78P (CTG→CCG)	recA	DNA recombination/repair protein RecA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/35);  total (31/35)

CGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTG  >  NC_000913/2823295‑2823780                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    cGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGcggcgtcagc                                                                                                                                                                                                                                             >  1:270612/1‑251 (MQ=255)  gCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCAACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGGTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGcggcgtcagcg                                                                                                                                                                                                                                            >  1:481891/1‑251 (MQ=255)   ccTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGcggcgtcagcgt                                                                                                                                                                                                                                           >  1:204171/1‑251 (MQ=255)           ttCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTAc                                                                                                                                                                                                                                   >  1:547708/1‑251 (MQ=255)             ccGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTAcc                                                                                                                                                                                                                                  <  1:179634/250‑1 (MQ=255)              cGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCgg                                                                                                                                                                                                                                >  1:426276/1‑251 (MQ=255)                     ggCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTc                                                                                                                                                                                                                         >  1:116652/1‑251 (MQ=255)                      gcggcattGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGAGCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTcc                                                                                                                                                                                                                        <  2:591899/244‑1 (MQ=255)                           aGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTcc                                                                                                                                                                                                                   >  2:566282/1‑251 (MQ=255)                             ggccgccACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGt                                                                                                                                                                                                                 <  2:158925/250‑1 (MQ=255)                                cgccACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGt                                                                                                                                                                                                                 >  2:437306/1‑248 (MQ=255)                                    aCGGAGCCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCTCAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCAGCGATAAACGCACAGGTTTTACCTTCTCGCGGCGCTGCGGCGATCACCTGCGGCGGCAGCGTGGTTTTACCGGAAGATGCCGGTCCGTAGATTTc                                                                                                                                                                                                          <  2:243516/251‑1 (MQ=255)                                       gAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGTGTCCAGCGCGTGTTCAGCATCGATAAACGCAGAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGGAGATTTcgac                                                                                                                                                                                                       <  2:629763/251‑1 (MQ=255)                                              cgacgaTAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACgg                                                                                                                                                                                                <  1:547187/251‑1 (MQ=255)                                                            aCTGCGCCAGCACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCCCAGCAGGTTGTCGCTATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATACACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGCTCACCTGCGGCGTCAGCGTGGTTTTACCGGCAGATTCCGGTCCGTAGCTTTCGACGCTCCGGCCCATCGGCAGacc                                                                                                                                                                                  >  2:570867/1‑251 (MQ=255)                                                            aCTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCTCCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGacc                                                                                                                                                                                  <  1:17018/251‑1 (MQ=255)                                                               gcgcCAGAACGCTCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGaccacc                                                                                                                                                                               <  1:14569/251‑1 (MQ=255)                                                                cgcCAGAACGCGCCAGGGCGGCCCAGATTTCCCGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCTACGCCCAGTTTACGTGCGTAGATTGGTTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTTCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCt                                                                                                                                                                              <  2:80962/251‑1 (MQ=255)                                                                   cAGAACGCGCCCGGGCGGCACAGATTTTCAGTGCCTGCTCCCCGGTTTCCGGCTGGGGGCCCAGCAGGGTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTTGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGcc                                                                                                                                                                           <  2:485788/251‑1 (MQ=255)                                                                   cAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGTTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGCCCCATCGGCAGACCACCTGcc                                                                                                                                                                           >  2:340929/1‑251 (MQ=255)                                                                      gaCGCTCCAGGGCGTCATAGATTTCCATTGCCGTCTCGCCGTTGGCCTGCGGTGAGCACAGCAGGTTGTCGAGATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAGCGCACAGGTTTGACCTTCTCGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAATATGCCGTACCGTAGAGTTCTACGATACGGCCCATCGGCAGACCGCCTGCCCCa                                                                                                                                                                        <  2:574107/250‑1 (MQ=255)                                                                      aaCGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCa                                                                                                                                                                        <  1:64073/251‑1 (MQ=255)                                                                           gcCAGGGCGTAACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAgcgc                                                                                                                                                                   >  2:407078/1‑251 (MQ=255)                                                                            ccAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGcgcg                                                                                                                                                                  <  2:343875/251‑1 (MQ=255)                                                                               gggCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGata                                                                                                                                                               <  1:566282/251‑1 (MQ=255)                                                                                    tCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATcc                                                                                                                                                            >  1:49992/1‑249 (MQ=255)                                                                                            ttCCAGTGCCTGCTAGCCGGCGTCCGACTGGGAGCACAGCAGCTTGTCGATATCGACCCCCAGTTTACGTGCGTAGATCGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCCCGCTGCGCTGCGGCGCTCACCTGCGGCGTCAGCGTGGTTTTACCGGACGATTCCGGTCCGTAGATTTCGCCGATACGGCCCATCGGCAGACCACCTGCCCCCAGCGCGATATCCCGTGAAAGCg                                                                                                                                                  >  2:597962/1‑251 (MQ=255)                                                                                               cAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAAc                                                                                                                                               >  2:384295/1‑251 (MQ=255)                                                                                                 gTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCg                                                                                                                                             <  2:110563/251‑1 (MQ=255)                                                                                                 gTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCg                                                                                                                                             >  1:331805/1‑251 (MQ=255)                                                                                                      tGCTCGCCGGTGTCCGGCTGGAAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGt                                                                                                                                           >  2:517038/1‑248 (MQ=255)                                                                                                                 gtCCGGCTGGGAGCACAGCAGGGTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTcc                                                                                                                              <  1:316450/250‑1 (MQ=255)                                                                                                                          ggAGCACAGCAGGTTGTCGATATCGAAGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGAGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATgg                                                                                                                    >  1:598292/1‑251 (MQ=255)                                                                                                                            aGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATAGCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGaa                                                                                                                  <  2:431000/251‑1 (MQ=255)                                                                                                                                      gTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCAc                                                                                                        >  2:151148/1‑251 (MQ=255)                                                                                                                                            gATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGgc                                                                                                  <  1:466597/251‑1 (MQ=255)                                                                                                                                               atCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGCTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGCAAGATTCCGGTCCGTAGATTTCGACGCTACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGgcgc                                                                                                >  1:302987/1‑250 (MQ=255)                                                                                                                                                    cGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCCGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCatgctg                                                                                          >  1:414170/1‑248 (MQ=255)                                                                                                                                                             tACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAATCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGATCCttt                                                                                  <  2:429759/250‑1 (MQ=255)                                                                                                                                                              aCGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTAc                                                                                <  1:22962/251‑1 (MQ=255)                                                                                                                                                              aCGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTAc                                                                                >  2:347632/1‑251 (MQ=255)                                                                                                                                                                 tGCGTAGATTGGGTCAAGCGCGTGTTCAGCATCGATAAACGCACCGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCACCAAGCGCGATATCCAGTGAACGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGCGCCTTTCCCa                                                                              >  2:233226/1‑250 (MQ=255)                                                                                                                                                                  gCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCaa                                                                             >  2:409067/1‑250 (MQ=255)                                                                                                                                                                      aGATTGGGTCCCGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGCAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCCAGCGCGATATCCAGTGAAAGCGAACCGGCAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGt                                                                        >  2:522281/1‑251 (MQ=255)                                                                                                                                                                      aGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTg                                                                         <  1:392314/250‑1 (MQ=255)                                                                                                                                                                      aGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGt                                                                        >  2:340440/1‑251 (MQ=255)                                                                                                                                                                           gggTCCAGCGCGTGTTCAGCATAGATCAACGCACAGGATTTAACTACACGCAGCGCTGCGGAGATCACCTGCGGCGTCAGCGTGGTTTTAACGGAAGATACCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCCAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTACCACATCCATGGAACGGACTTAACCCCGGCGCATGATGGAGCCTTTACCGAATTGTTtctc                                                                   >  2:82867/1‑251 (MQ=255)                                                                                                                                                                             gTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCa                                                                  >  1:119996/1‑250 (MQ=255)                                                                                                                                                                                cAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCt                                                              <  1:166706/251‑1 (MQ=255)                                                                                                                                                                                  gcgcGTGTTCAGCTTCGATAAACGCACGGGTTTTGCCTTCACGCTGCGCGTCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCACCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTGCCACATCCATGGAACGGTCTGCACCCAGGCGCATGATGTATCCTTTACCAAATTGTTTCTCAATCTgg                                                            <  2:527104/251‑1 (MQ=255)                                                                                                                                                                                      gtgtTCATCATCTATAAACGCACAGGTTTTCCCTTCACGCTCCGCTGCGGCGATCACCTGCGTCATCAGCGTTGTTTTACCGGAAGATTCCGGTCCGTATATTTCGACGATACGGCCCATCGGCAGACCACCTTCGCCAAGCGCGATATCCAGTGAAATCGAACCTGTAGATATGGTTTCCTCATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCa                                                        <  1:87684/251‑1 (MQ=255)                                                                                                                                                                                            aGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATCTCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACACAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGtgctg                                                  >  1:421887/1‑251 (MQ=255)                                                                                                                                                                                             gcagcGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGtgctgc                                                 <  2:144620/247‑1 (MQ=255)                                                                                                                                                                                                        cGCACAGGTTTTCCCTTCACGCGGCGCTGCGGCGATCCCCTGCGGCGTCCGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTTCTGCCGCCCACGCtt                                      <  2:113724/251‑1 (MQ=255)                                                                                                                                                                                                          cacaGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAGCCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCtttt                                    >  1:87989/1‑250 (MQ=255)                                                                                                                                                                                                               gtgttACCTTAACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACGACCTGCCCCAAGCGCGATATCCATTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCATGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCGGGCCCAGTGCTGCCGCCACCGCTTTCtgttt                               <  2:630518/248‑1 (MQ=255)                                                                                                                                                                                                                     ccTTCACGCTGCGCTGCGGCGATCACCTGCGACGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATCTCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCCGGCGCATGATGGAGCCTTTACCACATTGTTTCTCAATCTGTCCCAGTGCTGCCGCCAACGCTTTCTGTTTGttttt                         >  2:389862/1‑250 (MQ=255)                                                                                                                                                                                                                               gcgctgcgGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAg                 <  1:347632/249‑1 (MQ=255)                                                                                                                                                                                                                                cgcggcggctATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGACACCGGTCCGTAGAGTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGGTTGTTTTGGTCTACAGCCa              <  2:53590/247‑1 (MQ=255)                                                                                                                                                                                                                                   tgcgGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGCTAGCCAttt           >  1:328756/1‑251 (MQ=255)                                                                                                                                                                                                                                    gcggcgATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCAtt            <  2:49992/249‑1 (MQ=255)                                                                                                                                                                                                                                    gcggcgAACCCCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGCCGATCCGGACAATCGGCAGACCACCTGCACCAACAGCGATAACCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGCACGGTCTTCACCCAGGCGCAAGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCAtttt          >  2:560046/1‑251 (MQ=255)                                                                                                                                                                                                                                      ggcgATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGCTGGAGCCTTTACCAAATTGTTTCTCACTCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCCttttt         >  2:340750/1‑250 (MQ=255)                                                                                                                                                                                                                                      ggcgATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGGTAGCCCttttt         >  2:389955/1‑250 (MQ=255)                                                                                                                                                                                                                                         gATCACCCGCGGCGTCAGCGGTGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTTCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTc     <  2:408478/251‑1 (MQ=255)                                                                                                                                                                                                                                          agcACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATATGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCAGGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTcc    <  1:389393/249‑1 (MQ=255)                                                                                                                                                                                                                                           cccccTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCTTAGATTTCGACGATACGGCCCATCGGCAGACCCCCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATCGCCATTTTTACTCCt   <  2:56772/248‑1 (MQ=255)                                                                                                                                                                                                                                            cACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTg  <  1:340440/251‑1 (MQ=255)                                                                                                                                                                                                                                            cACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTg  <  1:27383/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    CGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTG  >  NC_000913/2823295‑2823780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑