Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,827	C→T	L152F (CTT→TTT)	evgS →	sensor histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,827	0	C	T	100.0%	84.9 / NA	28	L152F (CTT→TTT)	evgS	sensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

AGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAATATT  >  NC_000913/2484600‑2485073                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       agcaACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCtt                                                                                                                                                                                                                                 >  1:443873/1‑251 (MQ=255)                  aTTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGt                                                                                                                                                                                                                <  1:474200/251‑1 (MQ=255)                              tattTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAATCCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTc                                                                                                                                                                                                     <  1:605494/250‑1 (MQ=255)                                                   gCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGCAGCAAATTAcccccc                                                                                                                                                                                  >  1:425586/1‑248 (MQ=255)                                                               aaaTTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAAACAAACCATTGATAATTTCCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCCCCCCAAAACCAGTAAATATTGCGCGGGTAGCAAATTACCCCCCAGACGAGGTGATtc                                                                                                                                                                    <  2:206526/250‑1 (MQ=255)                                                                 aTTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATtcatc                                                                                                                                                                 <  1:54852/251‑1 (MQ=255)                                                                    aaCACTCCGGGAAGAAGCAGATCATCAAAAAGCAATGGACGCGCTTACTGAAGGTGAAGTCGATATAGTGTTATCCCATTTAGCTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACAGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCACATTACCCCCCAGACGAGGTAATTCatcaat                                                                                                                                                              >  2:70979/1‑251 (MQ=255)                                                                       acTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCAt                                                                                                                                                           >  2:393748/1‑251 (MQ=255)                                                                        cTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACAAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCAtt                                                                                                                                                          >  1:263801/1‑251 (MQ=255)                                                                                                     aaTGGACGCGCTTTCAGAAGGTGAAGGTGATATAGTTTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGGAATTCATCAATCAAATCCAAAAGCTACAATTATCTCTTTTACaa                                                                                                                             <  2:361386/251‑1 (MQ=255)                                                                                                      aTGGACGCGCTTGCAGAAGGTGAAGGCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAAGAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACaaa                                                                                                                            <  2:263801/251‑1 (MQ=255)                                                                                                       tGGACGCGCTTGCATAAGGTGACGTCGATATCGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGCTACTTACCTTTCCTGCGCTTGTAACCACCTTTCCCGACTCAATGCGCCCTCTTACCTCACCAAACCCATTACATTTTGCTCGGGTCGCAACTTACCCCCCAGACGCGGTAATTCATCCATCATTTCCAAAATCAACAATTCTCTCGTTTACAAAt                                                                                                                           >  2:184187/1‑251 (MQ=255)                                                                                                                   cAGAAGGTGAAGTCGATATAGTGTTATCGCATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTTCAACCAAATCATTGATAATTCCCGTTCCGGCGCTTGTAACCACCTGTCACGGCCCAATGCGACCGCTTACCTCACCAAAACCAATAAATATTGCTCGGGTAGAAAATTACCTCCCAGACGATGTAAATCAGCAAGCAGTTCCAAAAGCAACAAGTATCTCTTTCACAAACTTATATCAGGCa                                                                                                               <  2:550728/251‑1 (MQ=255)                                                                                                                    aGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAACCCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGAGCGCTTACCTCACCAAAACCAGTAACTATTGCTCGGGTAGCAACTTACCCCCCAGACGAGGGAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                                                                              >  1:269367/1‑251 (MQ=255)                                                                                                                      aaGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTTCCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTGCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTa                                                                                                            <  2:260929/251‑1 (MQ=255)                                                                                                                             aGTCGATATAGTGTTATCACATTTAGTTACTGCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAGCCAGTAAATATTGCTCGGGGAGCAAAGTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCg                                                                                                     <  1:309174/251‑1 (MQ=255)                                                                                                                                                      gTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACtt                                                                            <  1:28869/251‑1 (MQ=255)                                                                                                                                                       ttACTTAGCCGCCTCTTACTAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGCCGCTGGTAACCCCCTTTCACGACTCAATGCGGCAGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGATGGTCACAATGATTACttt                                                                           >  2:298784/1‑251 (MQ=255)                                                                                                                                                         aCTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAt                                                                         >  1:590143/1‑251 (MQ=255)                                                                                                                                                                 gccTCTTAATAATGACATTGCTGCAACCAATCCATTGATAATTACCTTTCCGGCGCTGGTAACCTCCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCGCTTTTACAAATTTATATCAGGCATTAGCATCCGTCGCAGATGGGCACAATGATTACTGTATTGgtagta                                                                 <  2:425586/251‑1 (MQ=255)                                                                                                                                                                    tctTAATAATGACATTGCTGCAACAAAACCATTGCTAATTACCTTTCCGGCGCTGGTAACCAACTTTCACGCCTCAATGCGCCCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGCGGAAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTCGCATCCGTCTCAGATGGGCACAATGCTTACTTTATTGGTAGTAAc                                                               >  2:55653/1‑250 (MQ=255)                                                                                                                                                                       taataaTGACATTGCTGCAACCAAACCATTGATACTTACCTTTCCGACGCTGGTAACCACCTTTCACGACTCAATGAGACCGCTTACCTCACCAAAACAAGTAAATATTGCTCGGGTAGCAAATTACCACCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAATAAcatcc                                                           >  1:541646/1‑250 (MQ=255)                                                                                                                                                                        aataatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcatca                                                           <  1:613138/250‑1 (MQ=255)                                                                                                                                                                          taatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACGCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                                                                              <  1:463191/197‑1 (MQ=255)                                                                                                                                                                          taatGACATTGCTGCAACCAAACCATTGATAATTACATTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                                                                              >  2:463191/1‑197 (MQ=255)                                                                                                                                                                                            caaaccaTTGATAATTATCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCAACAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCGCTTTTACAAATTTATATCAGGTATGAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTccc                                      <  1:281351/251‑1 (MQ=255)                                                                                                                                                                                                          ttACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAACTTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcactcac                        >  1:613015/1‑251 (MQ=255)                                                                                                                                                                                                                                caccTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCATCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAAtatt  <  1:584762/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       AGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAATATT  >  NC_000913/2484600‑2485073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑