Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,477,233	A→G	S133P (TCG→CCG)	dsdC ←	DNA‑binding transcriptional dual regulator DsdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,477,233	0	A	G	100.0%	126.6 / NA	38	S133P (TCG→CCG)	dsdC	DNA‑binding transcriptional dual regulator DsdC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/22);  total (16/22)

ATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCT  >  NC_000913/2476988‑2477475                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   aTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAAt                                                                                                                                                                                                                                                 >  1:62564/1‑249 (MQ=255)     gTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACgg                                                                                                                                                                                                                                           <  2:417241/251‑1 (MQ=255)                gTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCgtg                                                                                                                                                                                                                                >  2:737/1‑251 (MQ=255)                                                           aGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTATTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCCCCTAATGCGGGCACCAACCAGCATTTGTCGATagg                                                                                                                                                                                     >  1:458531/1‑250 (MQ=255)                                                                aaTTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGcc                                                                                                                                                                                <  1:8814/251‑1 (MQ=255)                                                                   tACCGTGTTGGGTAAAGCATGTCGTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCgg                                                                                                                                                                              <  2:16825/250‑1 (MQ=255)                                                                    aCCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCggg                                                                                                                                                                             <  1:73348/250‑1 (MQ=255)                                                                                        tCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCAAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTccc                                                                                                                                                         <  2:376611/250‑1 (MQ=255)                                                                                           ttGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGAt                                                                                                                                                      <  1:482516/250‑1 (MQ=255)                                                                                                 gTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCt                                                                                                                                               <  1:737/251‑1 (MQ=255)                                                                                                      ccGGGCTACATACTGGCAGGCTTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAAAGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGGATACAGAGGTAACGTTCCCGATAACTCCTGAttt                                                                                                                                          >  1:165027/1‑251 (MQ=255)                                                                                                       cGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATAAAGAGTTAACGTTCCCGATAACTCCTGAtttt                                                                                                                                         >  1:157880/1‑251 (MQ=255)                                                                                                       cGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGCGATGAGTGAGTTGCGCTGACGGCGCATCATCACAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAAGAGACGGATACCGGCGTGTAAAGTCACCTTAAGCGGGAACCCACCAGCATTGGGCGATAGTGGGCCGGGAATAACGGGTTAACGTTCCCgcatactaccagattt                                                                                                                                         >  2:225397/1‑236 (MQ=255)                                                                                                             aCATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTTCGCGGACGGCGTATCATCAAAGGAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACTTTGTCATTACCGGTGAGCACGGTGAGCGGAATAGACGGATACCGGCTTGTAAAGTCACCTAATGCGGGCAGCAACCAGCAGTGGGCGATAGAGGGCCGGGAATAGAGAGTTAACGGTCCCGAGAACACCttagtgttgata                                                                                                                                    <  2:594911/250‑12 (MQ=255)                                                                                                              cATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACGCCTGATTTTTGATATc                                                                                                                                  <  2:62564/251‑1 (MQ=255)                                                                                                               aTACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCATCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCa                                                                                                                                 <  1:547530/251‑1 (MQ=255)                                                                                                               aTACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGAAAATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTAATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAATCAACTAATGAGGGCACCAACCAGCATTGGGCGCAAAAAGGCCGGGAATACAGAATTAAAGATCCCGAAAACTCCTGATTTTTGATATc                                                                                                                                  >  2:48056/1‑250 (MQ=255)                                                                                                                 aCTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCaa                                                                                                                                <  1:485481/250‑1 (MQ=255)                                                                                                                      cAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCAACTAATGCGGGCACCACCCAGCATTGGGAGATAGAGGGCCGGGCATACAGAGTTAAAGTTCCCGATAACTCCTGATTTTTGATATCAAGAAttt                                                                                                                          >  1:36129/1‑251 (MQ=255)                                                                                                                         gATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCt                                                                                                                       <  1:228735/251‑1 (MQ=255)                                                                                                                                          gAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGAGAACTCCGGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGc                                                                                                      <  2:152249/251‑1 (MQ=255)                                                                                                                                                      tgagtTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGCGCACGGTGAGCGGAATAGCCGGATACCGGCGTGTAAAGTCACCCAATGCGGGCACCAACCAGCATTGGGCGGTCGAGGGCCGGGAATACAGAGTTAACGATCCCGCGAACTCCTGATTTTTGATTTCAAGAATTTCCTGGTTCTGGGTATCCATCGACGATTTTAgc                                                                                          >  1:410483/1‑251 (MQ=255)                                                                                                                                                              gcTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATa                                                                                  <  1:541650/251‑1 (MQ=255)                                                                                                                                                               cTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGGATACAGAGTTAAAGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGGTCAGGGGATACAGCGACGATTTTAGAGCCCAATa                                                                                  >  1:547049/1‑250 (MQ=255)                                                                                                                                                                  aCGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCCGCATTGGGCGATAGAGGGCCGGGGATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATTCAGCGACGATTTTAGCGCCCAATAAac                                                                               >  1:450270/1‑250 (MQ=255)                                                                                                                                                                         atcatcAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTcc                                                                       <  2:119861/251‑1 (MQ=255)                                                                                                                                                                             tcaAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAATCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAAAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTcccccc                                                                   >  2:503943/1‑249 (MQ=255)                                                                                                                                                                               aaaGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGt                                                                 <  1:102565/251‑1 (MQ=255)                                                                                                                                                                                     gATCGCCAAATCGATTCCGGCCCGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGGTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGGATCCAGCGAAGATTTTAGCGCCCCATAAACACGTTTCCCCTCGTGCGtt                                                            >  1:485560/1‑250 (MQ=255)                                                                                                                                                                                     gATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGTGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTa                                                           <  1:82350/251‑1 (MQ=255)                                                                                                                                                                                           cAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTaa                                                          >  2:4685/1‑246 (MQ=255)                                                                                                                                                                                           cAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTaa                                                          <  1:4685/246‑1 (MQ=255)                                                                                                                                                                                                  aTTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCg                                              <  1:143560/251‑1 (MQ=255)                                                                                                                                                                                                            gTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGGTAATTCCACTTTGCGATGGtaacga                                    <  2:224222/251‑1 (MQ=255)                                                                                                                                                                                                               gCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGgaacgaac                                  <  1:90319/250‑1 (MQ=255)                                                                                                                                                                                                                              cATTACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGGATCCAGCGACGATTTTAGCGCCCAATAAACACGTGTCCCCTCGTGCGGTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGccc                  <  2:331679/251‑1 (MQ=255)                                                                                                                                                                                                                                ttACCAGTGAGCACGGTGAGCGGAATAGACGGATACCGGCGAGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGCTTTTTGATATCAAGAATTTCCTGGGTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGAGCGTTAATTCCCCTTTGCGATGGGAACGAACAAACAAATGAATGCCCa                 >  1:557046/1‑250 (MQ=255)                                                                                                                                                                                                                                              ggTGAGCGGAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGCTAGAGGGCCGGGGATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCCCTTTGCGATGGGAACGAACAAACAACTGAATGCCCCATTCTTCTTCCAGCt  >  1:612710/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   ATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCT  >  NC_000913/2476988‑2477475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑