Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,410,419	G→A	L312L (CTG→TTG)	yfbS ←	putative transporter YfbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,410,419	0	G	A	100.0%	184.3 / NA	60	L312L (CTG→TTG)	yfbS	putative transporter YfbS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/23);  total (37/23)

CCAGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAG  >  NC_000913/2410176‑2410656                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              ccAGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATc                                                                                                                                                                                                                                        >  1:496423/1‑251 (MQ=255) ccAGCATACCGATCAGTTTCCAGTGACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAACACCAATTTCGCGCACCGATTTACCATTCAGTTCTGACTCAGGCATTAATGAAATCTCTGCCATGCCCACATCAAGTGCCTG‑GTCCGAAAAATCCTCGCCGCGCAATACCATc                                                                                                                                                                                                                                        >  1:28149/1‑251 (MQ=255)   aGCATACCGATCAGTTTCAAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACAACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGACTTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTAGCCGCGCAATACCATCgg                                                                                                                                                                                                                                      >  1:435213/1‑251 (MQ=255)   aGCAGACCGATCAGTTTCCAGTTCCCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCgg                                                                                                                                                                                                                                      <  1:400848/251‑1 (MQ=255)      aTACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTc                                                                                                                                                                                                                                   >  1:622550/1‑251 (MQ=255)      aTACCGATCAGTTTCCAGTGACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAAGCAGTTCTGACGCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GGCAGAAAAATACTCGCCGCTCAATTCCATCGGCTc                                                                                                                                                                                                                                   <  2:240380/251‑1 (MQ=255)              cAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATc                                                                                                                                                                                                                                        >  2:98944/1‑238 (MQ=255)              cAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATc                                                                                                                                                                                                                                        <  1:98944/238‑1 (MQ=255)                     cacttACCCACAACCTGGATGATATCGCCCACCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCCCGCCATTGCGCTTTAGCCCCACCACATTCAGTCAGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCCATCAGTTCTGACTCAGGAATTAATGAAATCTATGCCATGCCCACCTCAAGAGCCTG‑GTCAGACAAATACTCGCCGCGCAATAACATCGGCTCCAGCAAttactaact                                                                                                                                                                                                                    >  2:338598/4‑244 (MQ=255)                        atACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCCATCAGTTCTGACTCAGGAATTAATGAAATATCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCAAGCAATTGCTCACTAc                                                                                                                                                                                                                  >  2:340359/2‑250 (MQ=255)                          aCCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGTCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGGAGCGGTTACGAAAGCCAATGTCGCGCACCGAGTTACCAATGAGTTCGGAGTCAGGGAGTTAGGAAAGCCCTGCCATGCCCACATCAAGTGC‑TGTGGCAGAAAAATTCTCGCCGCGCAATACCACCGGCGCCAGGAATGGCTCACTACaa                                                                                                                                                                                                                <  2:628152/250‑1 (MQ=255)                                   cAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTCCAAAATTGCCgg                                                                                                                                                                                                       >  1:39516/1‑250 (MQ=255)                                     ggATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCgg                                                                                                                                                                                                       >  1:110603/1‑248 (MQ=255)                                                        cagAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGAc                                                                                                                                                                                  >  1:258064/1‑250 (MQ=255)                                                               tCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAAt                                                                                                                                                                          >  2:227737/1‑251 (MQ=255)                                                                       cAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCa                                                                                                                                                                     <  1:137904/248‑1 (MQ=255)                                                                       cAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCa                                                                                                                                                                     >  2:137904/1‑248 (MQ=255)                                                                        aGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAAc                                                                                                                                                                 <  2:466483/251‑1 (MQ=255)                                                                         gCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACg                                                                                                                                                                <  1:207862/251‑1 (MQ=255)                                                                            aaCCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAAtt                                                                                                                                                                                                                           >  2:398835/1‑189 (MQ=255)                                                                            aaCCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAAtt                                                                                                                                                                                                                           <  1:398835/189‑1 (MQ=255)                                                                                    aGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACACAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCAcgcgcgcg                                                                                                                                                     >  1:554520/1‑251 (MQ=255)                                                                                      cgccacgccaTTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGa                                                                                                                                                    >  2:1729/1‑250 (MQ=255)                                                                                               aTTGCGCTTTAGCCCCAACACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAgca                                                                                                                                           >  2:454449/1‑248 (MQ=255)                                                                                                  gcgcTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATGTCGCGCACCGATTTACCAAGCAGTTCTGACGCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACAGAGCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAAcc                                                                                                                                        <  2:441414/250‑1 (MQ=255)                                                                                                   cgcTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAAcccc                                                                                                                                      <  1:423707/251‑1 (MQ=255)                                                                                                       ttAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCAtt                                                                                                                                   <  1:160424/250‑1 (MQ=255)                                                                                                               accacATTCAGGCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCAc                                                                                                                          <  1:34821/251‑1 (MQ=255)                                                                                                                    aTTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATa                                                                                                                      >  1:591908/1‑250 (MQ=255)                                                                                                                                    gTACGCAAAACAATTTCGCGCACCGATTTAAAAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGACATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAAcgccg                                                                                                     >  2:253596/1‑251 (MQ=255)                                                                                                                                    gTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAAcgccg                                                                                                     >  1:463622/1‑251 (MQ=255)                                                                                                                                         aaaaCCAATTTCGAGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGACATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGc                                                                                                >  2:84455/1‑251 (MQ=255)                                                                                                                                           aaCCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGC‑AGCGtt                                                                                             <  1:26934/251‑1 (MQ=255)                                                                                                                                              cAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCCGCGTTc                                                                                            >  2:31974/1‑250 (MQ=255)                                                                                                                                                  ttCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAaca                                                                                        <  1:364110/250‑1 (MQ=255)                                                                                                                                                       caacagATTTACCAATCAGTTATGACTCAGGAATTAATGAAATCACTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCTCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATCCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATg                                                                                  <  2:28149/246‑1 (MQ=255)                                                                                                                                                          ccGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACg                                                                               >  1:211414/1‑251 (MQ=255)                                                                                                                                                                tACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAgc                                                                          <  2:283879/250‑1 (MQ=255)                                                                                                                                                                            cggacccAGGAATTAATGAAATCTCTGCCATGCCCGAATAAAGGGCCTG‑GTCAGAAAAATCCTCTCCGCGCAAGACCAGCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGAGCGACAGCAGCCGCAGACATATCAATAAGTAAAACGGCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACAGATGAAGTTATCGCGATACCGCTc                                                              <  2:359528/244‑1 (MQ=255)                                                                                                                                                                                cTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGt                                                          >  1:307825/1‑250 (MQ=255)                                                                                                                                                                                 tCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCCCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATACCGCTCACGTa                                                         >  2:308023/1‑250 (MQ=255)                                                                                                                                                                                     gAATTAATGAAATCTCTGCCATGCCCACATCACGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTg                                                    >  2:77150/1‑251 (MQ=255)                                                                                                                                                                                             gAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGatcatc                                            >  1:333454/1‑251 (MQ=255)                                                                                                                                                                                                aTCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATcc                                           >  2:36144/1‑249 (MQ=255)                                                                                                                                                                                                       ccATGCCCACATCAAGGGCATG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTg                                   >  1:173108/1‑250 (MQ=255)                                                                                                                                                                                                            cccACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt                             <  1:420258/251‑1 (MQ=255)                                                                                                                                                                                                            cccACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt                             >  1:578022/1‑251 (MQ=255)                                                                                                                                                                                                             ccACATCAAGGTCCTG‑GACAGAAATATACTAGCCGCGCAATACCATCGGCCCCCGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGACACCCGCGCGAAATTAAGAAACCCCATTATCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGCTGACGTTAGCGCCATAACGCTCACGTAATTTGAGGTCGTCCAGCCGTTGACCAATc                            >  2:86677/1‑251 (MQ=255)                                                                                                                                                                                                             ccACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATACCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATc                            >  1:52593/1‑251 (MQ=255)                                                                                                                                                                                                             ccACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATc                            <  1:541567/251‑1 (MQ=255)                                                                                                                                                                                                                 aTCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGATACAGCAATTGCTCACTACAAAATTGCCGGAGATAGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCAAGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAAAGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTg                        >  1:367300/1‑251 (MQ=255)                                                                                                                                                                                                                      gggCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGtt                                                                             <  1:378266/193‑1 (MQ=255)                                                                                                                                                                                                                      gggCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGtt                                                                             >  2:378266/1‑193 (MQ=255)                                                                                                                                                                                                                        gCCTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATGGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCGCGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCGCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATcc                 <  2:577268/251‑1 (MQ=255)                                                                                                                                                                                                                         ccTG‑GTAAGCAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCAGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCCATCCg                >  1:504504/1‑251 (MQ=255)                                                                                                                                                                                                                          cTG‑GTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGCTCCgg               >  2:139355/1‑251 (MQ=255)                                                                                                                                                                                                                                  gAAAAATACTAGCCGCGCAATCCCATCGGTGCCAGCAATTGCTCAATACAAAAAGGCCGTAGATCGACATCAGCCGCAGACATATCAATAAACAAAACGTCACGCGCGCGAAATGCAGAAACCCCATGAACGTTCCCGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGAGGACGTTAGCGCCACGGCGCGCACGTAATTGGTGATCAGCCAGCTGTTGACCAATCATTGGCCGTCCGGGGCGAAt        <  2:39269/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaaaaTACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAAt        >  1:409965/1‑250 (MQ=255)                                                                                                                                                                                                                                        tACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAACTTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGCCGTTAGCGCCATAACGCTAACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGcc    >  2:275303/1‑249 (MQ=255)                                                                                                                                                                                                                                         aCTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAg  <  2:591908/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              CCAGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTG‑GTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAG  >  NC_000913/2410176‑2410656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑