Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,384,808 C→T K305K (AAG→AAA)  yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,384,8080CT97.7% 130.7 / ‑4.5 43K305K (AAG→AAA) yfbKIPR002035/DUF3520 domain‑containing protein YfbK
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (24/18);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

ATTCAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTGAAC  >  NC_000913/2384562‑2385049
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
aTTCAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTtc                                                                                                                                                                                                                                                 <  2:523037/250‑1 (MQ=255)
  tCAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTtc‑gt                                                                                                                                                                                                                                              <  1:236226/250‑1 (MQ=255)
      tACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGATGTAATTGCTATTCACCACGCCAAACGTCGAAAGAGTAACACCAGACTCCCGATGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGATAAAGTCACCGTCAGTGGCTAATAAAATGAGATTGATGCCGCACTTAATAAACCCTTTC‑GTCGCct                                                                                                                                                                                                                                         >  1:232951/1‑251 (MQ=255)
              gcgcTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTAGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGa                                                                                                                                                                                                                                    <  1:284909/248‑1 (MQ=255)
                  ttCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCCCGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCACCGTTACAGTCACCGTCAGTGGCTAATAAAATGCGCTTGATGCCGCCCTTACTAACCCCTTTC‑GTCGCCTGCTGATAAGccc                                                                                                                                                                                                                             >  2:64625/1‑250 (MQ=255)
                    cAGAGAGGGTATAAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGt                                                                                                                                                                                                                           >  2:585848/1‑251 (MQ=255)
                     agagagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGtt                                                                                                                                                                                                                          >  2:91169/1‑251 (MQ=255)
                             tATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCg                                                                                                                                                                                                                  >  2:362833/1‑251 (MQ=255)
                                 aaTGTAGCTGTAGTTGCCGTTCCCAACCTCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTAACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCAc                                                                                                                                                                                                              >  2:141158/1‑251 (MQ=255)
                                               tGCCTTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTATTTGCTATTCCCCACGCAAAACGTCGACAGAGTAACCCCAGCCTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAAGGCCAACGTTAAAGTCCCCTTCAGTGGCTAATAAAATGCGATGGATGCCGCCCTTAATAACCCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACt                                                                                                                                                                                                 <  2:478737/250‑1 (MQ=255)
                                               tGCCGTTACAAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCCGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTg                                                                                                                                                                                                >  1:529104/1‑251 (MQ=255)
                                                gCCGTTACCAACATCGGAAATTCGCACCATCATTGCCTCGTTGTAATTGCTATCCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTg                                                                                                                                                                                                <  2:188335/250‑1 (MQ=255)
                                                                   aTTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCTCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATc                                                                                                                                                                            >  2:364367/1‑251 (MQ=255)
                                                                                 ggccgcGTTGTAATTGCTATCCCCCCCGCCAAACGTAGACAGAGGAACACCAGACTCCCGCTGGTTTTGGACCATGGATCAAATCGATTGTGGAGCGTCAATGCCAACGTAAAAGTAACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTAAATAAACCCTTTC‑GTCGCCGGCTGATAAGCCAGTGCAAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATGGCGGCATTa                                                                                                                                                               <  2:574684/245‑1 (MQ=255)
                                                                                            aaTTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCtt                                                                                                                                                    >  2:190264/1‑250 (MQ=255)
                                                                                                   actCCCCACGCAAAACGTCGCAAGAGTAACCCCAGACTCCCTCTGTTTTTTGACCTTTGATTCAAGGGTTTTTGGATCGTCAATTCAAACGTTAAAGCCCCCTTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTAAATAACCCCTTTC‑GCCCCCTGCTTTTAAGCCAGTTCCAGCCCGTCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCTTTATGACTcc                                                                                                                                            <  2:501189/249‑1 (MQ=255)
                                                                                                   aTTCCCGACGCCAAACGTCGACGGAGTAGCACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATAGATTTTGGATCGTCAATGCCAACGTTCAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTACTTTCCGCCTTATGACTcc                                                                                                                                            >  2:113235/1‑251 (MQ=255)
                                                                                                                      gACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgcaatgc                                                                                                                         >  2:25486/1‑251 (MQ=255)
                                                                                                                           attaccacCAGACCCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATAc                                                                                                                    <  1:362833/246‑1 (MQ=255)
                                                                                                                                         cccGCTGTTTTTTGACCATTGATTCAATCGATTTGGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGAGGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAg                                                                                                        <  1:25486/249‑1 (MQ=255)
                                                                                                                                          ccGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTACTACAATGCGATTGATGCCGCCCTTACTAAACCCTTTC‑GTCGCCTGCTGATACGCCAGTTCCAGCCCGGCCCCGCCATTGGTCCTGCCTTCGGCATCCAGCGAATCTATTGCGGCATTACTTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCACTACGGGAGTCGCCAGCGt                                                                                                     >  1:299555/1‑251 (MQ=255)
                                                                                                                                            gCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCAAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAg                                                                                                   <  1:428373/251‑1 (MQ=255)
                                                                                                                                              tGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGCTAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGt                                                                                                 >  1:497837/1‑251 (MQ=255)
                                                                                                                                               gTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTc                                                                                                >  2:113218/1‑251 (MQ=255)
                                                                                                                                                   tttGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACg                                                                                             <  1:510762/250‑1 (MQ=255)
                                                                                                                                                      gACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATgg                                                                                         <  1:364367/251‑1 (MQ=255)
                                                                                                                                                               ttCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTAt                                                                                >  1:1272/1‑251 (MQ=255)
                                                                                                                                                                 cAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATcc                                                                              >  1:502772/1‑251 (MQ=255)
                                                                                                                                                                     cGATTTTGGATCGTAAATGCAAACGTTAAAGTCACCGTAAGTAGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATTCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCt                                                                          >  2:282904/1‑251 (MQ=255)
                                                                                                                                                                      gtttttGGATCGTCAATGCCACCGTTAAAGTCACCGTCAGTGGCTAATAAAATGAGATTGATGCCGCCCTTAATAACCCCTTTC‑GTCGCCGGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGAGGGCAATGTTATCCTGCTc                                                                         <  2:497837/249‑1 (MQ=255)
                                                                                                                                                                      gATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTc                                                                         >  1:621822/1‑251 (MQ=255)
                                                                                                                                                                                    aaTGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGa                                                           <  1:91169/251‑1 (MQ=255)
                                                                                                                                                                                       gCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGAcc                                                         <  1:220088/250‑1 (MQ=255)
                                                                                                                                                                                         cAACGTAAAAGTCACAGTAAGTGGTTAATAAAATGCGATTAATGCCGCCCTTAATAAACCCTTTC‑GTCGCCGGCTGATAAGCCAGTTCCAGCCCGGCCCCGCCATTGGTACTGCCTTCGGCATCCAGCGAAGCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGAGAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCaat                                                      <  1:64625/251‑1 (MQ=255)
                                                                                                                                                                                          aaCGTGAAAGTCACCGTCAGTGGCTAAAAAAAGGCGATTGATGCCGCCCTAAAAAAACCCTGTC‑GTCGCCTGCTTATAAGCCAGTGCCAGCCCGGCACCGCCATTGGGACTGCCTTCGGCAGCCAGCGAATCAATGGCGTCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAAGACGGGAGTCGCCATCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCaata                                                     <  1:261225/251‑1 (MQ=255)
                                                                                                                                                                                                  aGTCACCGTCAGTGGCTACTAACATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGGGTCGCCGGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAAc                                             >  1:485214/1‑251 (MQ=255)
                                                                                                                                                                                                   gTCACCGTTAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCAATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACg                                            >  1:359492/1‑251 (MQ=255)
                                                                                                                                                                                                                       aaaTGCGATTGCTGCCGACCTTAATAAACCCTTTC‑GCCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGGGGCaa                         >  2:526680/1‑250 (MQ=255)
                                                                                                                                                                                                                                tGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGACCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCAAGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTtcatc                >  1:394223/1‑250 (MQ=255)
                                                                                                                                                                                                                                  aTGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTtcatc                >  1:463167/1‑248 (MQ=255)
                                                                                                                                                                                                                                    gccgccCTTAA‑AAACCCCTTCCGTCGCCTGCTGATAAGCCAGTCCCAGCCCGGCCCCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGaaa           <  1:352897/251‑1 (MQ=255)
                                                                                                                                                                                                                                      cgccCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATc         >  1:743/1‑251 (MQ=255)
                                                                                                                                                                                                                                             aataaCCCCTTTC‑GTCCCCTGCTGATAAGCCAGTTCCAGCCCGTCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTGAAc  <  1:406836/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
ATTCAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTGAAC  >  NC_000913/2384562‑2385049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑