Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,942,635	C→T	intergenic (‑52/‑27)	znuA ← / → znuC	Zn(2(+)) ABC transporter periplasmic binding protein/Zn(2(+)) ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,942,635	0	C	T	100.0%	69.6 / NA	22	intergenic (‑52/‑27)	znuA/znuC	Zn(2(+)) ABC transporter periplasmic binding protein/Zn(2(+)) ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/8);  total (14/8)

GGCGCAGTGAATAATCATGTTCTGAAGCGCCGTCAGGAAGTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCG  >  NC_000913/1942388‑1942864                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                      ggCGCAGTGAATAATCATGTTCGGAAGCGCCGTCAGGAAGTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTGAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGGGATAT‑ATAACATTTTATGa                                                                                                                                                                                                                                   <  1:14025/251‑1 (MQ=255)             aatCATGTTCTGAAGCGCCGTCAGGAAGTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAg                                                                                                                                                                                                                         <  1:79053/250‑1 (MQ=255)                               tgTCAGGAAGTAAAACCTCTGTTTCTGATACCCCATCATCACTGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACCGCGGCATCTGCGCCCTGTGGTGCACCTCCCCAGAGAGAGACAGATAAGGCTTGGAAAAGAAGAGTTCATTTATTGTTA‑ATAAAGCGGCCAATAATCGTAATGCGTGTGAGAAGTGGGATATTAAAACATTTTATGACTGATACAAAACTTAAATTAAccaaaca                                                                                                                                                                                                       >  2:628786/2‑244 (MQ=255)                                gTCAGGAAGTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACa                                                                                                                                                                                                       >  1:198116/1‑249 (MQ=255)                                   aGGAAGTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATAGTAATGAATATGAGAAGTGTGGTATTATAACATTTTATTACTAATGCAAAACTAAAATTAACATGACAAGTCt                                                                                                                                                                                                  >  2:419339/1‑251 (MQ=255)                                           aaaCCTCTGTTTCTGTTACCACATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGAGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTccc                                                                                                                                                                                          >  1:540230/1‑251 (MQ=255)                                                                   cAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACCTTTTATGACTACTGCAAGACTAAAATTAACATGTCAAGTCTgg                                                                                                                                                                                                >  2:122383/1‑221 (MQ=255)                                                                   cAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTgg                                                                                                                                                                                                <  1:122383/221‑1 (MQ=255)                                                                                           cAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGAtg                                                                                                                                          <  1:552406/251‑1 (MQ=255)                                                                                             aCGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGAtgtg                                                                                                                                        >  1:398105/1‑251 (MQ=255)                                                                                                ggTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCAAGAGAGCGGCGGATAATGCCGCGAAAAGAAGGGATTTTTTAT‑GTACCATAATGCGGCCAATAATCGTAATGAATATGGGAAGTGTGATATTATAACATTTTATGCCTCCTGCCAGACTAAAATTAACATGACAAGTATGGTTTCACTGGAAAATGTCCCGGATTCTTTTGGCCAACGACGCGTCCTCTCTGCTGTGTCg                                                                                                                                     >  1:318139/1‑251 (MQ=255)                                                                                                 gTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGc                                                                                                                                    <  1:419339/251‑1 (MQ=255)                                                                                                               aCAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAACCCt                                                                                                                      >  2:255793/1‑251 (MQ=255)                                                                                                                                 gCCTGTGTTGCACCTCCCCAGAGAGCTGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATGTTGACTTTa                                                                                                    <  1:198887/251‑1 (MQ=255)                                                                                                                                                                  aaTGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTg                                                                   >  1:18476/1‑251 (MQ=255)                                                                                                                                                                     gctgcGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGGCGACACTGGt                                                                 <  2:540230/250‑1 (MQ=255)                                                                                                                                                                          gAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGCTGTGTCGCTGGAACTTAAACCTGGAAAACTTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGCCACTGGTACGGGt                                                           >  1:414652/1‑251 (MQ=255)                                                                                                                                                                              agaagCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGAGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGACTGGTTTCACTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCAGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTg                                                       >  1:335023/1‑251 (MQ=255)                                                                                                                                                                                    gTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCggg                                                 <  2:198116/251‑1 (MQ=255)                                                                                                                                                                                                        tGCGACCAATAATCGTAATGAATATGTGACGTGTGATCTTATACCATTTTATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGACAATGTCTCGGTTTCTTTTGGCCAACGCCGAGTCCTCTCTGATGTGTCGCTGGAACTTACACCTGGAAAAATTTTGCCTTTACTTGGGCAACATGGCGCAGGTCAGTCGACACTGGAACGGGTAGTGCTCGGGCTGGTAACACCCGATGa                                >  2:101485/1‑249 (MQ=255)                                                                                                                                                                                                                               atGAGAAGTGTGATATTATAACATTTTCTGAATACTGCAAGACTAAACTTAACATGAAAAGTCTGGTTTACCTGGAAAATGTCTCGGTTTCTTTTGGACAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACATGGAAAACTTTTGACTTTACTTGGTCCCAATGGAGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGaaa        >  2:390021/1‑250 (MQ=255)                                                                                                                                                                                                                                    acgtgtGATATTATAACATTTTATGACTACCGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAACAATTTTGACTTTACTTGGGCCAACTGGCGACGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGCTGACGGGGTTATCAAGCGCAACGGCAAACTgcg  >  2:275910/3‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                      GGCGCAGTGAATAATCATGTTCTGAAGCGCCGTCAGGAAGTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAACGGCGGCATCTGCGGCCTGTGTTGCACCTCCCCAGAGAGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTAT‑GTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCG  >  NC_000913/1942388‑1942864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑