Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,629,247	C→T	I11I (ATC→ATT)	ydfZ →	putative selenoprotein YdfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,629,247	0	C	T	100.0%	90.3 / NA	31	I11I (ATC→ATT)	ydfZ	putative selenoprotein YdfZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/13);  total (18/13)

AGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCG  >  NC_000913/1629007‑1629489                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   aGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGc                                                                                                                                                                                                                                           >  1:282452/1‑250 (MQ=255) aGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGc                                                                                                                                                                                                                                           >  2:186552/1‑250 (MQ=255)            gACTGGTTTAGCGAAGAGTCCTTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTCTGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGGGTTATg                                                                                                                                                                                                                               >  2:124604/1‑251 (MQ=255)                 gTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGtt                                                                                                                                                                                                                            >  1:423259/1‑249 (MQ=255)                      gCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCgga                                                                                                                                                                                                                      >  1:594908/1‑249 (MQ=255)                      gCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCa                                                                                                                                                                                                                     <  2:423259/251‑1 (MQ=255)                           gagTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCACAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTACCCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGt                                                                                                                                                                                                                >  2:266096/1‑251 (MQ=255)                                                        gggTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATAc                                                                                                                                                                                   <  2:290904/251‑1 (MQ=255)                                                               agTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAACATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAggg                                                                                                                                                                            >  1:399537/1‑250 (MQ=255)                                                               agTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGCAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGt                                                                                                                                                                            >  2:503814/1‑251 (MQ=255)                                                                    tttCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTTATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCGAGGACGACCTACGATCGTAACCGTAACGCAAGTACCACTGGCAGCCGGGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCGGTCGATTGATACTGAAGGTCTGa                                                                                                                                                                        <  2:318206/250‑1 (MQ=255)                                                                              cTTATGGATCATGCATGCATCAAAAAAGATGTGAGATCGATCAAAAACAAAAATTATTTCACTCGACAGACGTATTTATATTGCGCCCGTTACGTGAGCTTCGCATGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGCTATGGTTAGCGGCACCGGTCCCACTGGCAAGATCCTGTCGATTGATACTGAAGGCCTGACAGCTGAGCa                                                                                                                                                              >  2:455481/1‑250 (MQ=255)                                                                                tATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCaa                                                                                                                                                             <  1:186552/249‑1 (MQ=255)                                                                                   ggCTCCTGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGACCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATccg                                                                                                                                                        >  1:609085/1‑251 (MQ=255)                                                                                     cTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATccgcc                                                                                                                                                      >  1:144837/1‑251 (MQ=255)                                                                                          gcatgcatCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATGTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGa                                                                                                                                                 <  1:503814/251‑1 (MQ=255)                                                                                                atCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAAcc                                                                                                                                           <  1:421547/251‑1 (MQ=255)                                                                                                atCAAAAAAGAGGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGACCCGGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAAcc                                                                                                                                           <  2:508913/251‑1 (MQ=255)                                                                                                     aaaaGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAgt                                                                                                                                      <  1:36849/251‑1 (MQ=255)                                                                                                      aaaGATGTGCGCTTGATCAACAACAAAAAATATTTCCCTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGCGCAAATCCGCCGCGGAAAAACCGTAgtt                                                                                                                                     >  2:594066/1‑251 (MQ=255)                                                                                                                            acaaaaaATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAAc                                                                                                               <  2:282452/251‑1 (MQ=255)                                                                                                                                       ttCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTg                                                                                                    >  1:47538/1‑251 (MQ=255)                                                                                                                                             cGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGATAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCCCTGGCAGCCGTGGTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATTCTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTg                                                                                              <  1:118676/251‑1 (MQ=255)                                                                                                                                                     gTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGCTTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGtct                                                                                      >  1:554766/1‑251 (MQ=255)                                                                                                                                                      gaTTTAAATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTAGGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGAGCCTGTCGATTGATACTGAAGGTCTGACCGCGGAGTAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGtctc                                                                                     <  1:287915/250‑1 (MQ=255)                                                                                                                                                                  gcCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTa                                                                          >  2:170594/1‑250 (MQ=255)                                                                                                                                                                        tACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACCCCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCgtg                                                                    <  2:152444/250‑1 (MQ=255)                                                                                                                                                                                                                   gacgacCTACGATCGTAACCGTAACGCAATTAACACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGAATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAat                         >  1:545614/1‑250 (MQ=255)                                                                                                                                                                                                                         cTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGc                  >  2:171461/1‑251 (MQ=255)                                                                                                                                                                                                                              aTCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACgg             >  1:42992/1‑251 (MQ=255)                                                                                                                                                                                                                                         aacGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcgcg  <  1:407496/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   AGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCG  >  NC_000913/1629007‑1629489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑