Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,588,149	G→A	L50F (CTC→TTC)	ydeR ←	putative fimbrial protein YdeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,588,149	0	G	A	100.0%	64.0 / NA	21	L50F (CTC→TTC)	ydeR	putative fimbrial protein YdeR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/9);  total (12/9)

AACCGTTTTGCTATCGCCATTTTTTAACGCAGCATCCTGGTCATCCCTCAGCTCTATCTGAATATTTTCGGCAGTACCTTCATTTTTGTAATAACCCGTATTGTCAGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAGATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCAAAACGTAGCTAACAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCG  >  NC_000913/1587916‑1588390                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  aaCCGTTTTGCTATCGCCATTTTTTAACGCAGCATCCTGGTCATCCCTCAGCTCTATCTGAATATTTTCGGCAGTACCTTCATTTTTGTAATAACCCGTATTGTCAGTTGAACCTGTCACGATTGCCGTCTCTGCACTTGTTTCAACCGGACAAGCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAGCGTTAGcttctt                                                                                                                                                                                                                                  <  2:233469/251‑1 (MQ=255) aaCCGTTTTGCTATCGCCATTTTTTAACGCAGCATCCTGGTCATCCCTCAGCTCTATCTGAATATTTTCGGCAGTACCTTCATTTTTGTAATAACCCGTATTGTCAGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGcttctt                                                                                                                                                                                                                                  >  1:210670/1‑251 (MQ=255)       tttGCTATCGCCATTTTTTAACGCAGCATCCTGGTCATCCCTCAGCTCTATCTGAATATTTTCGGCAGTACCTTCATTTTTGTAATAACCCGTATTGTCAGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCCGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGttt                                                                                                                                                                                                                            >  1:536693/1‑251 (MQ=255)          gCTATCGCCATTTTTTAACGCAGCATCCTGGTCATCCCTCAGCTCTATCTGAATATTTTCGGCAGTACCTTCATTTTTGTAATAACCCGTATTGTCAGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGa                                                                                                                                                                                                                          <  1:411382/250‑1 (MQ=255)                          aaCGCAGCATCCTGGTCATCCCTCAGCTCTATCTGAATATTTTCGGCAGTACCTTCATTTTTGTAATAACCCGTATTGTCAGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGc                                                                                                                                                                                                         <  1:578080/251‑1 (MQ=255)                             gcagcaTCCTGGTCATCCCTCAGCTCTATCTGAATATTTTCGGCAGTACCTTCATTTTTGTAATAACCCGTATTGTCAGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGAc                                                                                                                                                                                                      >  2:522928/1‑251 (MQ=255)                                                                            aCCTTCATTTTTGTAATAACCCGTATTGTCAGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTGTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGTCCTGGAGAGTTg                                                                                                                                                       <  2:295638/251‑1 (MQ=255)                                                                                           ataaCCCGTATTGTCAGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCtagactggccccct                                                                                                                                        >  2:241219/1‑238 (MQ=255)                                                                                                    aTTGTCAGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGAGCCAGCCAGATGCAGAACAAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTATCAGTGATAGAGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCAAAACGt                                                                                                                                 >  1:558335/1‑249 (MQ=255)                                                                                                          aGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTCATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGCAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCCAAACGTAGCTAAc                                                                                                                          >  2:97661/1‑250 (MQ=255)                                                                                                          aGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCAAAACGTAGCTAAc                                                                                                                          <  1:97661/250‑1 (MQ=255)                                                                                                                         gATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTCGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCCAAACGTAGCTACCAGGAACCTCTTGTGa                                                                                                           >  1:44396/1‑250 (MQ=255)                                                                                                                         gATTGACGTCACTGCGCTTGTTTCAACCGGACAATCGGTTAATGACAAAGTACTATTGTGCCAGCAAGCTGCCGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGGCTCCCCGACCATTAACAGTGATCGTGACATCGGCTGCCTGGCGCGTTGCTGTGAATAACGCGCAAAACGGAGCTAACAGGAACCTCTTGTGa                                                                                                           >  2:361520/1‑250 (MQ=255)                                                                                                                              ccGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCCGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCAAAACGTAGCTAACAGGAACCTCTTGTGAAGTCtt                                                                                                     >  2:265017/1‑251 (MQ=255)                                                                                                                                       cACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACATTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACTCGCAAAACGTAGCTAACAGGAACCTTTTGTGAAGTCTTTTCATATCt                                                                                            <  2:608819/251‑1 (MQ=255)                                                                                                                                                       aCAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCAAAACGTAGCTAACAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGaa                                                                            <  2:466859/251‑1 (MQ=255)                                                                                                                                                                         aGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCAAAACGTAGc                                                                                                                              <  1:444987/183‑1 (MQ=255)                                                                                                                                                                                            gATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCAAAACGTAGCTAACAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTcc                                       <  2:210670/251‑1 (MQ=255)                                                                                                                                                                                                                   aTTGCGCGTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATCGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCAAAACGTAGCTAACAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGCGTTg                >  1:417804/1‑251 (MQ=255)                                                                                                                                                                                                                       cgcgTATAAAGATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCCGGGTTTAGCGACTACCCGACCATTAACAGTGCTAGCGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCAAAACGTAGCTAACAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACtt            >  1:290795/1‑251 (MQ=255)                                                                                                                                                                                                                                 gATCCCCGAAATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCAAAACGTAGCTAACAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCg  >  1:599377/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  AACCGTTTTGCTATCGCCATTTTTTAACGCAGCATCCTGGTCATCCCTCAGCTCTATCTGAATATTTTCGGCAGTACCTTCATTTTTGTAATAACCCGTATTGTCAGTTGAACCTGTCACGATTGCCGTCACTGCACTTGTTTCAACCGGACAATCGGTTAATGACAAAGTAATATTGTGCCAGCCAGATGCAGAACCAGGTTGTTGCAGATTGCGCGTATAAAGATCCCCGAGATTAACGTTAGCTTCTTTGGTTTGAATAGTGCAGGGTTTAGCGACTACCCGACCATTAACAGTGATAGTGACATCGGCGGCCTGGAGAGTTGCTGTGAATAACGCGCAAAACGTAGCTAACAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCG  >  NC_000913/1587916‑1588390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑