| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,494,930 | C→T | E48K (GAA→AAA) | ydcI ← | DNA‑binding transcriptional dual regulator YdcI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,494,930 | 0 | C | T | 96.0% | 71.4 / ‑3.3 | 25 | coding (142/924 nt) | ydcI | DNA‑binding transcriptional dual regulator YdcI |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (11/13); minor base . (0/1); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAAT > NC_000913/1494689‑1495155 | tttggttgCTGATGAAACTGACCTATAACCGAAGTGAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGGAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCATGTT‑ATTCAATg < 2:272809/246‑1 (MQ=255) ttgttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTc < 2:128682/250‑1 (MQ=255) aaTATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATAGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGttt < 1:601465/250‑1 (MQ=255) ccccAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTTCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCcg < 1:56696/251‑1 (MQ=255) cAGTGCCGCAGTAGGTAGTGAACCAACCCTGACGCCATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAACTTAAGGGTTTCAGCcgcgc > 2:55796/1‑251 (MQ=255) gCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCa > 2:320414/1‑250 (MQ=255) gtaggtagTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGt > 2:46109/1‑251 (MQ=255) taggtagTGCACCAACCCTGACGCCATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCACGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTACGGGTTTCAGCCGCGAGCCCCAAAGtt > 2:533887/1‑251 (MQ=255) cTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGc > 1:44095/1‑251 (MQ=255) tttACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAAc > 1:60202/1‑251 (MQ=255) aTGAAGCGACCGGCCGGCAGTGTTGAAGGCTTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGTGTAAGCTAAGTTGCCCCTCCTGACGACCACGCCCAAACAAGCGAGCGTCAGTCAGCTGCTCCA‑GTTTATTCAAGGTTTTAGAGGGCGCAGGTTGACTCTAATTAAGGGTGGCAGCCGCGCGCCCCTAAGTTCCTTGTTGTGCGACTGCTACGAATGTATGAAGGCGGCGCAAACGGATgc < 2:273862/251‑1 (MQ=255) gAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGCACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCACACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCttg > 2:57102/1‑205 (MQ=255) gAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCttg < 1:57102/205‑1 (MQ=255) gCGACCGGCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTGTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCTACAGCTACGAATGTATGAAGGTGTCGCAAACGGATGCGCTGa < 2:536533/251‑1 (MQ=255) gACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACAGCATGCGTTAAACATTGTTCGCCGGGTAAGGAAAGTTGCGACCCATGTCGACCACGCTCACACAAGCGAGCGCCCGTCAGCTGATCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGACGCGCGCCCCAAAGTTACTTGTTGAGCGACAGCTACGAATGTATGAAGGTGGAGCAAACGGATGCGCTGACt > 1:90568/1‑251 (MQ=255) aCCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCGTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTa < 2:459129/251‑1 (MQ=255) gATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTAtttttt < 1:46109/250‑1 (MQ=255) gAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGAt < 1:290811/251‑1 (MQ=255) gctgtttaagggaaaTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCCCTA‑GTTTATTCAATGTTCGAGAGAGCGCATGTTGACTCAAATTAAGGGTTTCAGCCACGCGCCCCAAAGTTCCGTGTTGTGCTACAGCTACGAATGTATGTAGGTGGAAAAAACGGATGCGCGGACTAAACTGTCTTATTTGTTCCATAAGCTATATTAAATACGAAGCGGTTTCGccgagtagt < 2:612185/244‑9 (MQ=255) gggTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGa > 2:615647/1‑249 (MQ=255) ccACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCaa < 2:60202/251‑1 (MQ=255) gCTCAAACAAGCGAGCGCCAGACCGCTGCTCCA‑GTTTCTTCACTGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAACCGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCaaatt > 2:461000/1‑248 (MQ=255) aaCAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATa < 1:540607/174‑1 (MQ=255) aaCAAGCGAGCGCCAGTCAGCTGCTCAA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCACACGGATGCGCTGACTAAACAGACTATTTTTTTCCATa > 2:540607/1‑174 (MQ=255) cgagcgCCAGTCAGCTGCTCCA‑GTTTATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAAt < 1:590163/251‑1 (MQ=255) | TTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCA‑GTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAAT > NC_000913/1494689‑1495155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |