Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,859	T→C	Y110C (TAT→TGT)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,859	0	T	C	100.0%	72.3 / NA	27	Y110C (TAT→TGT)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/14);  total (13/14)

GACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑TAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATC  >  NC_000913/1358615‑1359104                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gACGGTGCCAACGGGTGCTCCGTTATGCAGGCCCTGAGCTACCAGGAGGATAAATACCGCCATGATGGAGATTGGCACCAGTACAAACAGGGTATTGAAGTTAGCGAGCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAGGGCGACGAAGGTTACCACCCGCACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCca                                                                                                                                                                                                                                                 <  2:345756/251‑1 (MQ=255)       gCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCGCACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCagcagc                                                                                                                                                                                                                                            <  1:32707/250‑1 (MQ=255)                 tctcCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCATGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGc                                                                                                                                                                                                                                  <  2:612544/250‑1 (MQ=255)                     cTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGGCGAAGGTTACCACCCACACCCACGGCGGCAATTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGCTAAACATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGAcc                                                                                                                                                                                                                             >  1:350844/1‑251 (MQ=255)                          tGCAGTCCCTGAACTACAAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGCTAAAATGGCGACGAAGGGTTCCACCCACACCCACCGCGGCCCTTCCGGGTAGAGGGCGGAGAGAAAGATTTTTGCCAACAAGACGGTTATCATCGGCAAAAAGAAA‑CAATCCAACAGCGATGACCCACCGACCATaa                                                                                                                                                                                                                         >  1:450514/1‑250 (MQ=255)                                 ccTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGGTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAACCGACCATAAATCCGACg                                                                                                                                                                                                                 >  1:91442/1‑251 (MQ=255)                                      aCTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCgg                                                                                                                                                                                                            <  1:336934/251‑1 (MQ=255)                                            aGGAAGCTAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAAt                                                                                                                                                                                                      >  1:510697/1‑251 (MQ=255)                                                 gATAAACACCTCCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTATCGACCAGGTTGACGCTCTGCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGATAGATAGATTTTCTCCAACAAGACGTTAATCAGCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACt                                                                                                                                                                                                 <  1:359801/251‑1 (MQ=255)                                                       caccacCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACAGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTgcg                                                                                                                                                                                           <  1:136673/251‑1 (MQ=255)                                                            ccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGACGAAGGTTACCACCCACACCCACGGCGGCAAATTCGGGAAGAGGGCGGGGGGAGAGATTTTCTCCAACAAAAAGTTAATCATCGGCCAAAAGAAAACAAACCA‑CCGCGATGAACCGACGAACACAAATCCGACGGGCGGGATAATCGACGTTTGagcgg                                                                                                                                                                                        >  1:329104/1‑248 (MQ=255)                                                             caTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAgg                                                                                                                                                                                     <  2:183193/251‑1 (MQ=255)                                                                               cAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGATCTTCAGATTAGCGGCGGTTAAAAAGGCGAAGAAAGGTACCACCCCCACCCACGGCGGCACTTCCAGGGAGAGGGCGGGGAGATATATTTTCGCCAACAAGACGATAATCATCGGCACAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGGCCCTAAATCCGACGGGCGGGTTACTAGACTTTTGCGCGGAGGGATAGGcccaaacaccct                                                                                                                                                                   >  2:399252/1‑241 (MQ=255)                                                                                                             cAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGc                                                                                                                                     <  1:479383/251‑1 (MQ=255)                                                                                                              aGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGCAGGTTACCACCCACACCCACGGCGGCACTTCCGGGGAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGGTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAACCGACCATAAATCCGACGTGGGGGTTAATCGACTTTTGCGCGTAGGGATAGGCCGAACCGGCCCCCGGCAACTGGCGGACCAGTTTGCCGTAGCt                                                                                                                                    >  2:292495/1‑251 (MQ=255)                                                                                                                       ctctTCAGATTCGCGGCGGCTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGGAGCGGGAGGAGAGACAGCTTTTCGCCAACCCGACGTTAATCCTCGGCAACAAGAGA‑CAATCCAGCAGCGATGACCAGCCGGCCATAAATCCCACGGGCGGGTTAATCGAATTTTGCGCGTACGGATAAGCCGGACCGGCATCCGGAAAATCGCGAACCAGATTGACGTAGCTAATAGCggg                                                                                                                           >  1:384515/1‑250 (MQ=255)                                                                                                                           tCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTa                                                                                                                          <  1:292495/248‑1 (MQ=255)                                                                                                                              gATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGTCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCTACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGc                                                                                                                    <  1:630520/251‑1 (MQ=255)                                                                                                                                                                     acacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTg                                                                              >  1:20172/1‑250 (MQ=255)                                                                                                                                                                       ccccacGGCGGCCCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCAAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGGAGGTATAGGCCGAACCGGCCCCCGGAAACTGGCGACCCAGTTGGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGAcc                                                                           <  2:510697/250‑1 (MQ=255)                                                                                                                                                                                       ccGGGCAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATAGGCAAAAAGAGA‑CAATCCAGCAGCGCTGACCAGCCGACCCTAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGaa                                                                                                                                                             >  2:413186/1‑153 (MQ=255)                                                                                                                                                                                       ccGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGaa                                                                                                                                                             <  1:413186/153‑1 (MQ=255)                                                                                                                                                                                            aaGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCCGTTTGCCGTAGCTGATAGAGGTAAACAGCACACCCGCCAGCGCCAGCAAATCGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAAt                                                      >  2:23944/1‑251 (MQ=255)                                                                                                                                                                                                gggCGGAGAGATAGATTGTCGCCAACAAGACATTAATGATCGGAAAAAAGAGA‑CAAGCCAGCAGCGATGACCAGCCGACCATAAACCCGACGTGCGGGTTAATCGACTTTGTCGCGTAGGTATAGGCCGAACCGGCCGCCGAAAACTGGCGAACCAGTTTGCTGTAGCGGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCa                                                  <  1:73460/251‑1 (MQ=255)                                                                                                                                                                                                             gatTTTCGACAACGAGCCGTTCATCATCGGCAAAAAGAGA‑CAATCCAGCCGAGATGACCAGCCGACCATCAATACGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTCGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATAcccaacgactcaaat                                      >  2:209949/1‑237 (MQ=255)                                                                                                                                                                                                                  ccGCCAACAAGACGTTAATCATGGGCAAAAAGAGA‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGTAGACAATACCAAAAGTATCAAATACGGTc                                <  1:179056/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gaga‑CAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGAATCAAATACGGACATCGGAGTGAGATAGGCCAGACCAatcatc  >  1:125625/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGA‑TAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATC  >  NC_000913/1358615‑1359104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑