Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	127.2 / NA	41	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/19);  total (22/19)

AGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTC  >  NC_000913/1337154‑1337633                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                aGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAACCGTAACTCTGGGCATCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGACGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGAATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑CCCACCTGTATTGGTAACTCTTAGCCTCTGCCCGATCCTATCGAACCGGCAATCGAAAAAGGCGATTTAAc                                                                                                                                                                                                                                       >  2:143662/1‑251 (MQ=255)        tGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggt                                                                                                                                                                                                                                >  1:452323/1‑251 (MQ=255)           tGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggtgcg                                                                                                                                                                                                                             >  2:435463/1‑251 (MQ=255)                gCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCt                                                                                                                                                                                                                        <  1:589021/251‑1 (MQ=255)                 cAGGCTTGATGGCGAAAAAAGCCGTAACTCTGGGCATCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGTTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTg                                                                                                                                                                                                                       <  2:142563/251‑1 (MQ=255)                  aGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGt                                                                                                                                                                                                                      >  2:453265/1‑251 (MQ=255)                          tGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAc                                                                                                                                                                                                              >  2:131994/1‑251 (MQ=255)                               aaaaaaGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTa                                                                                                                                                                                                          >  1:565855/1‑250 (MQ=255)                                     gCCGTAACTCTGGGCATCCAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAACGGCGATTTAACCGTCGGTGCGGGGCTGTCCGGCAACCGTCACTTTg                                                                                                                                                                                                    >  2:414015/1‑250 (MQ=255)                                             tctGGGCCTCAAGCGGAAACCATGGGTCAAAGCGTCGCTGGCACAGGGATCGAAAGTCGTTTCGGATTATCTGGCACAAGCGAAACTGACACCGTATCTCGACGTACTGGGGTTTAACCTTGTGGAATACGGTTGT‑ACCACCTGTATTGGTAAATCTGGGCCGCTGCCCGGTCCTATCGAAACGGCCATCAAAAAAGGCGATTTAAACGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGcagt                                                                                                                                                                                            >  1:357208/1‑247 (MQ=255)                                                      cAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCTGATCCATACGc                                                                                                                                                                                  >  2:464010/1‑251 (MQ=255)                                                                ccATGGGGCAAAGCGTCGCTGGCACCGGGTTCGAAATGCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTTGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTGTGAAGGCCGTATCCATCCGCTGGTTAAaac                                                                                                                                                                        <  2:167330/251‑1 (MQ=255)                                                                      gTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActg                                                                                                                                                                  >  2:169708/1‑251 (MQ=255)                                                                             cGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGcc                                                                                                                                                           <  1:75003/251‑1 (MQ=255)                                                                                    ggCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGGGAAACTGACCCCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgcc                                                                                                                                                      <  2:539676/249‑1 (MQ=255)                                                                                       aCCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTACCCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgccgccgc                                                                                                                                                 >  2:541994/1‑251 (MQ=255)                                                                                              tCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTTGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggtgg                                                                                                                                           <  1:387947/250‑1 (MQ=255)                                                                                                   gtccgatcttGATTATCTGGCAAAAGCGAAACTGACACCGGATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTAGTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTGTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCt                                                                                                                                     <  2:569400/242‑1 (MQ=255)                                                                                                    gTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTa                                                                                                                                    <  1:453265/251‑1 (MQ=255)                                                                                                            tGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGATTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCt                                                                                                                                     >  1:569400/1‑242 (MQ=255)                                                                                                             gATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGTTGGTTAACCTTTTGGGATACG‑TTGTTACCACCTGTATTGGTAACTCGGGGCCGCTGCCTGATCCTATCTAATCGTCAAGCAAAAAAGGTGATTTATCCGACGTTTCGGGGCGTTCCTTCAGCCGTAGGTTTGAAGGCCGTCTCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCTCCGCCGCTTGGGGTGGCCTATGCGCTg                                                                                                                             <  2:58228/249‑1 (MQ=255)                                                                                                             gATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTgg                                                                                                                            <  1:72200/250‑1 (MQ=255)                                                                                                                              gAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATc                                                                                                           >  1:112278/1‑250 (MQ=255)                                                                                                                                       acCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCtt                                                                                                 >  1:572361/1‑251 (MQ=255)                                                                                                                                                 gacgaTCTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCtct                                                                                       >  2:36191/1‑249 (MQ=255)                                                                                                                                                 gacgaACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGCTCCTATCGAAACGGCACTCAAAAAAGGCGATTTACCCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTAt                                                                                       >  1:85477/1‑251 (MQ=255)                                                                                                                                                  acgaACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATc                                                                                      >  1:116654/1‑251 (MQ=255)                                                                                                                                                    gaACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCgg                                                                                    >  2:39968/1‑251 (MQ=255)                                                                                                                                                      aCTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGcc                                                                                  >  1:7376/1‑251 (MQ=255)                                                                                                                                                         ggggTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCGGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCAt                                                                                <  2:189417/250‑1 (MQ=255)                                                                                                                                                           ggTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGAt                                                                             <  1:39968/251‑1 (MQ=255)                                                                                                                                                                 aCCTTGTGGGATACGGTGTT‑ACCACCTGGATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAAGCAAAAAAGGCGATTTAACCGTCGGTGCGGTGTTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGTTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTAACGGCCATGAGCGCaaa                                                                       <  2:303302/251‑1 (MQ=255)                                                                                                                                                                  ccTTGGGGGAAACGGTTGT‑ACCACCTGTAGTGGTAACGCTGGGCCGCTGCCCGATCCAATCGAAACGGCAATCAAAAAAGGCGATTGAACCGTCGGTGCGGTGCTGTCCGGCACCCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTTGCCTCGCCTCCGCTGGTGGTTGCCTATGCGCTGGCTGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAg                                                                      <  2:50518/251‑1 (MQ=255)                                                                                                                                                                                      accaccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGa                                                    >  2:51220/1‑250 (MQ=255)                                                                                                                                                                                               aTTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTg                                          <  1:147925/251‑1 (MQ=255)                                                                                                                                                                                               aTTGGTAACGCTGGGCCGCTGCCCGAGCCTATCTAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTTCGGTGCTGTCCGGCAGCCGTAGCTTTGAAGGCCGTATCCATCCGCTGTTTAAAACTAACTGGCTGGCCTCGCCGCCGCGGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGACTTCTGAGATTAGCGGCCATGATCGCAAAGGCGATCCTGTTTAGCTGTAAGATATCt                                           <  2:42544/250‑1 (MQ=255)                                                                                                                                                                                                                    cccGATCCTATCGAAACGGCAATCAACAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGCGGTTGCCTATGCGCTGGCGGGAAATATGATTATCAACCTGGCTTCTGAGCCTATCGGCCATTATCGAAACAACGATCCGGTTTATCTGAACGATATCTGGCACTCGGCACACGGAATTGc                     >  2:432068/1‑251 (MQ=255)                                                                                                                                                                                                                             aTCGAAACGGCAATCAAAAAAGGCGATTTAATCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCg              <  1:143662/249‑1 (MQ=255)                                                                                                                                                                                                                                gAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGa         <  1:153534/251‑1 (MQ=255)                                                                                                                                                                                                                                       cAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAAATATCTGGCCATCGGCACAAGAAATTGACCGTGCCGTAGAACAAGtc  >  2:610853/1‑251 (MQ=255)                                                                                                                                                                                                                                       cAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAAGATGAATATCAACCTGGCTTCTGAGCCTAGCGGCCATTATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGtc  <  1:415780/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                AGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGT‑ACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTC  >  NC_000913/1337154‑1337633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑