Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,329,638 G→A V103I (GTC→ATC)  sohB → S49 peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,329,6380GA85.0% 93.5 / 4.3 40V103I (GTC→ATC) sohBS49 peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base A (16/18);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGGCGATTGCCGCCATTATTGTCAATGTTGCTCAACGTAATAAACGCCAGCGTGGCGAGTTACGGGTCAACAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGCGGCGGTTACATGATGGCC  >  NC_000913/1329394‑1329883
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
tGCGGCGATTGCCGCCATTATTGTCAATGTTGCTCAACGTAATAAACGCCAGCGTGGCGAGTTACGGGTCAACAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTgg                                                                                                                                                                                                                                                  <  1:429109/250‑1 (MQ=255)
  cggcgATTGACGCCATTATTGTCAATGTTGCTCACCGTAATAAACGCCAGCGTGGCGAGTTACGGGTCAAAAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACAGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGAACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGCAAGCCAAAATGGGCGCGGTGGCAACTGACAGTAAACACC‑GCATCTGGGTg                                                                                                                                                                                                                                               >  2:605567/1‑251 (MQ=255)
     cgATTGCCGCCATTATTGTCAATGTTGCTCAACGTAATAAACGCCAGCGTGGCGAGTTACGGGTCAACAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTg                                                                                                                                                                                                                                            <  1:449469/251‑1 (MQ=255)
             gccATTATTGTCAATGTTGCTCAACGTAATAAACGCCAGCGTGGCGAGTTACGGGTCAACAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCGGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTaa                                                                                                                                                                                                                                    <  2:515760/251‑1 (MQ=255)
                         aaTGTTGCTCAACGTAATAAACGCCAGCGTGGCGAGTTACGGGTCAACAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATggc                                                                                                                                                                                                                        >  1:337452/1‑250 (MQ=255)
                               gCTCAACGTAATAAACGCCAGCGTGGCGAGTTACGGGTCAACAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACCTCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGcccc                                                                                                                                                                                                                  >  2:93718/1‑250 (MQ=255)
                               gCTCAACGTAATAAACGCCAGCGTGGCGAGTTACGGGTCAACAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCa                                                                                                                                                                                                                  <  1:456370/251‑1 (MQ=255)
                                  caaaggaataaACGCCAGCGTGGCGAGTTACGGGTCAACAAACCCAGCGAACAGTATAAGGAGAAGAAAGAAGAACCGGCCGCGGCGCTGATGGACTCACATCAGCAAAAAAAGGGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGa                                                                                                                                                                                                               <  1:615106/245‑1 (MQ=255)
                                  cAACGTAATAAACGCCAGCGTGGCGAGTTACGGGTCAACAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATg                                                                                                                                                                                                                >  1:50170/1‑250 (MQ=255)
                                          taaACGCCAGCGTGGCGAGTTACGGGTCAACAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACt                                                                                                                                                                                                       >  1:59817/1‑251 (MQ=255)
                                                          gAGTTACGGGTCAACAATCTCAGCGAACAGGATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCAGGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGAt                                                                                                                                                                                       <  2:50170/251‑1 (MQ=255)
                                                               aCGGGTCAACAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACg                                                                                                                                                                                   <  1:353844/250‑1 (MQ=255)
                                                                                           aaGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGa                                                                                                                                                      <  1:113613/251‑1 (MQ=255)
                                                                                              gagaTGAAAGAAGAACTGGCCGCTGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCa                                                                                                                                                   <  1:605567/251‑1 (MQ=255)
                                                                                                               ggCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACGCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTgt                                                                                                                                  <  2:337452/251‑2 (MQ=255)
                                                                                                                     ggcgCTGATGGACTCACCTCAGCAAAAACCGTGGCACAAAGCGCAGAAGAAACAGCATAAGCAAGAAGCCAACGCCGAACACGCGAAAGCCAAACTGGGAGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTCCGTGAAGAGATACCGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGaaatcc                                                                                                                            >  2:611823/1‑248 (MQ=255)
                                                                                                                     ggcgCTGATGGACTCACCTCAGCAAAAACAGTGGCACACAGCGCAGAAGAAAAAGCATCAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAACCTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGAAGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCACACCGCAGGACAAGGTTGTGATCCGTCTGGAAAGcc                                                                                                                            >  2:276166/1‑251 (MQ=255)
                                                                                                                        gcTGATGGCCTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGGGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCtg                                                                                                                         >  1:327742/1‑251 (MQ=255)
                                                                                                                            aTGGACTCACATCAGCAAAAAAAGTGGCACAACGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCtggtgg                                                                                                                     >  1:513793/1‑251 (MQ=255)
                                                                                                                                aCTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGgcatg                                                                                                                 <  1:160562/251‑1 (MQ=255)
                                                                                                                                                     ggCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATTGTGCATGGTTACGGGCTggc                                                                                             <  2:385381/250‑1 (MQ=255)
                                                                                                                                                     ggCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTggcg                                                                                            >  1:226496/1‑251 (MQ=255)
                                                                                                                                                     ggCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTggc                                                                                             <  1:100188/250‑1 (MQ=255)
                                                                                                                                                        acaAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACGGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCt                                                                                         <  2:594119/251‑1 (MQ=255)
                                                                                                                                                            aGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGc                                                                                     <  1:583676/251‑1 (MQ=255)
                                                                                                                                                                  gaagaaAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGc                                                                               <  2:327742/251‑1 (MQ=255)
                                                                                                                                                                      aaaaaGCATAAGCAAGAAGCGACGGCAGCAAAAGCGAAAGCCAAACTGGGAGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGACCCGTCTGGAAAGCACTGGTGGCATGGTGCATGGTTACGGGCGGGCGGCTTCGCAACTGCAGCg                                                                           >  2:496107/1‑251 (MQ=255)
                                                                                                                                                                              tAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGcgcg                                                                   >  1:152733/1‑251 (MQ=255)
                                                                                                                                                                                aGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAATCCCTGGTGGCATGGTGCATGTTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGAt                                                                 <  2:441468/251‑1 (MQ=255)
                                                                                                                                                                                              gcagcaAAAGCTAAAGCCAAACTGGGCGATGTGGCAACGGACAGTAAACCCC‑GCATCTGGGTGTTGGATTTTAAAGGCAGCATGGACGCCCATGAAGGAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCGGGTGGCATGGTGCATGGTTACGGGCTGGCGGCGTCGCAACTGCAGCGGCTGCGCGATAAAAACATTCCttt                                                   <  2:81914/251‑1 (MQ=255)
                                                                                                                                                                                                         gAAAGCCAAACTGGGCGAGGTGGCAACTGCCAGTAAACCCC‑GCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTAAGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCAGGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGAt                                                                 <  2:133209/226‑1 (MQ=255)
                                                                                                                                                                                                         gAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGTCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCAAGTTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGAt                                                                 >  1:133209/1‑226 (MQ=255)
                                                                                                                                                                                                                        ggAGGTGGCAAATGACAGTTAACCCC‑GCGTGTGGGTGCTGGATGGTAAAGGCAGCATGGAAGCCCATGAAGTGAACTCGCTGCGGGAAGAGATAACGGCTGTAGTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCGGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATGCCTTTAACTGTTACGGTAGACAAAGTCGCTg                         <  2:26309/250‑1 (MQ=255)
                                                                                                                                                                                                                        cGAGGTGGCAACTGACAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTg                         >  1:536500/1‑251 (MQ=255)
                                                                                                                                                                                                                          aGGTGGCAACTGACAGTCCACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGcc                       >  1:170960/1‑251 (MQ=255)
                                                                                                                                                                                                                           ggtagCAACTGACA‑TAAACCCCCGCGTCTGGGTACTTGATTTGAAATGCAGCATGGGCGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACGCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTTCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGGAGACAAAGTCGCTGCCa                      <  2:186262/247‑1 (MQ=255)
                                                                                                                                                                                                                                      aCAGTAAACCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCGTTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCCGCGGCGGCTAc           >  2:492768/1‑251 (MQ=255)
                                                                                                                                                                                                                                           aaaCCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGcggcgggatacaagt      >  1:612459/1‑242 (MQ=255)
                                                                                                                                                                                                                                           aaaCCCC‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGGGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATGCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGCGGCGGTTACatga       <  2:226496/250‑1 (MQ=255)
                                                                                                                                                                                                                                               ccc‑GCATCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTAAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGCGGCGGTTACATGATGGcc  <  2:318085/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
TGCGGCGATTGCCGCCATTATTGTCAATGTTGCTCAACGTAATAAACGCCAGCGTGGCGAGTTACGGGTCAACAATCTCAGCGAACAGTATAAGGAGATGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCC‑GCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCTGCGCGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGCGGCGGTTACATGATGGCC  >  NC_000913/1329394‑1329883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑