Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,325,054	G→A	W103* (TGG→TGA)	yciQ →	DUF2207 domain‑containing protein YciQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,325,054	0	G	A	100.0%	70.4 / NA	23	W103* (TGG→TGA)	yciQ	DUF2207 domain‑containing protein YciQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/15);  total (8/15)

TATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAA  >  NC_000913/1324809‑1325289                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            tATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCt                                                                                                                                                                                                                                        >  1:530280/1‑251 (MQ=255)  aTGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCtt                                                                                                                                                                                                                                       <  1:59213/251‑1 (MQ=255)    gCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGa                                                                                                                                                                                                                                     <  2:394185/251‑1 (MQ=255)                                      ggATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCTAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGATCCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGc                                                                                                                                                                                                   <  1:375416/251‑1 (MQ=255)                                                       gTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCtt                                                                                                                                                                                  <  2:207663/251‑1 (MQ=255)                                                                               ggggTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCa                                                                                                                                                          <  1:83791/251‑1 (MQ=255)                                                                               ggggTGCTCGACATCAAAGAAAATATTCAGTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCGTGCCTCAAGGCGACTATCATTATc                                                                                                                                                           <  2:320940/250‑1 (MQ=255)                                                                                       cGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTTTCAAATTCa                                                                                                                                                    <  2:313966/249‑1 (MQ=255)                                                                                       cGACATCAAAGAAAATATTCAGTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACTACTATGGATGCAGCCTGATTGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGTTATATCGGGTTAATGAGTATTGTCGTGTGCGATAAACAACGTGTCTTGCCTCAATGCGACGATCATTATCTAATTCAt                                                                                                                                                   <  2:413979/250‑1 (MQ=255)                                                                                                       atTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAAGTCATTATCAGGTTAAAAATg                                                                                                                                   <  1:523341/250‑1 (MQ=255)                                                                                                             tttCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTCCCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCt                                                                                                                            >  1:311337/1‑251 (MQ=255)                                                                                                              ttCAGGCGCGAAATCAGCAGATTAAGCACTGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTg                                                                                                                           >  1:511016/1‑251 (MQ=255)                                                                                                                      cgAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAggg                                                                                                                   <  1:243667/251‑1 (MQ=255)                                                                                                                        aaaTCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATACTGTTGGCGTCACCCGTGATGGTCTTCCTAACCCCTGACATCTTGACTGGCCTATCGGGTTAATGAGTATTGTCGTGGGCGATACACAACGTTTCTTGCCTCAAGACACCTATCATTATCAAATTAATTATCAGGTAAAACATGCTTTACTACGTGAggggg                                                                                                                 >  1:297305/1‑251 (MQ=255)                                                                                                                               cagATTCAGAACGGCTTTTATCGTGATTTACCACGCCTATGGCTGCAGCCAGAAGGGGATGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCCCGATGGTCTTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCCGGTTAAAAATGCTTTCCTGCGTGAGCGGGATTCTg                                                                                                           >  2:415933/1‑250 (MQ=255)                                                                                                                                agATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGAAGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGAt                                                                                                         <  2:80158/251‑1 (MQ=255)                                                                                                                                                       gATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCgg                                                                                  <  1:370335/251‑1 (MQ=255)                                                                                                                                                                             aGCCTGATGGTGACGCTGCACTGCTGAACTCTCATATTGGTGGCGTTACCCGTTATGGTATTCCTTAACCCTGACATCTTGACTGGCATATCTGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGTTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCTCTGGCCGTTTGAAAtt                                                            <  2:511683/251‑1 (MQ=255)                                                                                                                                                                                                              atatTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAat                           <  1:479450/251‑1 (MQ=255)                                                                                                                                                                                                                   gttgGCGTCACCCGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGc                      >  1:339319/1‑251 (MQ=255)                                                                                                                                                                                                                               cGTGATGGTATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCAtt          >  1:133267/1‑251 (MQ=255)                                                                                                                                                                                                                                       tATTCCTGAACCCTGACATCTTGACTGGCATATCGGGTTAATGAGAAATGTCGTGGGCGATAAACAACGATTATTGCCTCCAGGCGACTACCATTATCAAATTCATTATCAGGTCAAAAATGCTTTCATGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCAATGGCCGTTTGCAATTTATAAGACCCGTTTTTATCTCCAGTTCTCTATTATTGCGGGTAATCAATTTAGCgca   >  1:613307/1‑248 (MQ=255)                                                                                                                                                                                                                                        aTTCCTGAACCCTGACATCGTGACTGGCATATCGGGTTAAAGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCTGGTAATCCATTTAGCGaaa  <  2:520474/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            TATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAA  >  NC_000913/1324809‑1325289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑