Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,286,730 C→T L8F (CTC→TTC)  ychS → putative uncharacterized protein YchS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,286,7300CT95.8% 60.5 / NA 24L8F (CTC→TTC) ychSputative uncharacterized protein YchS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (10/13);  minor base G (0/1);  total (10/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTC  >  NC_000913/1286490‑1286970
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
gACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGGTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTTATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTg                                                                                                                                                                                                                                         <  2:326848/250‑1 (MQ=255)
   gcGTAAGTACCAGCGGGGGCGCGGCCGGCACGATGCGAATGTTAGGTCACATAGGCTCTTTTTCGGCGGGGTTGTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGTTTATCCGGCCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGGGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTTGGGGAAGGATTATTCAGCGCTgcgcg                                                                                                                                                                                                                                     <  2:275846/250‑1 (MQ=255)
   acGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGGGGGGGGAGGATTATTCAGCGCTgcgcg                                                                                                                                                                                                                                     >  1:130416/1‑251 (MQ=255)
                                       gAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACCGGGGGGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGGAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGAGGGGGGGGAAGGAGTATTCAGCGCTGCGCGATTCGCCCTTCGGGGCGTTGCCTGCGGCAACGctct                                                                                                                                                                                                 >  2:147058/1‑251 (MQ=255)
                                                 tCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAATTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGGCGTTGCCTGCGGCAACGGTCTCTCGCTgggg                                                                                                                                                                                       >  2:503624/1‑249 (MQ=255)
                                                                    gcggGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCTCTCAAGTCGAACCTTGGTc                                                                                                                                                                    <  1:129744/251‑1 (MQ=255)
                                                                                                    aGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGaa                                                                                                                                    <  1:421028/251‑1 (MQ=255)
                                                                                                          cGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTg                                                                                                                              >  1:304367/1‑251 (MQ=255)
                                                                                                                     cgcttATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAATCGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGTAAGCGATGGTGGTGGGGGAAGGTTTATTCAGCGCTGCGCGCTTCGCCCTACGGGGCGTTGCCGGCGGCAACGCTCGCGCGCTGGCGCTCAAGTCGATCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATGGCGGAtt                                                                                                                    <  1:101711/250‑1 (MQ=255)
                                                                                                                           cccGACCTAAAGGTGAGGATATTGTAGGCCCGGTAAACGTTGTGCCATCGGGCAAAAGTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGGGGGGGAAGGATTATTCAGCGCTGCGCGCTGCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCAAGTCGAACCTTGGTCGAAGTTTCTCATCCTTCCCCGGTTGGGCAGAATATTTGATTGCGGATTCGTTTgt                                                                                                             <  2:106712/250‑2 (MQ=21)
                                                                                                                              gACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTgaga                                                                                                           >  2:117120/1‑250 (MQ=255)
                                                                                                                              gACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGaa                                                                                                          <  1:117120/251‑1 (MQ=255)
                                                                                                                                  tACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAGTTcc                                                                                                      >  2:231513/1‑251 (MQ=255)
                                                                                                                                   aCAGGGGAGGAAATTGTAGGCGCGGTAACCGTGGTGCCATAGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTTAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCACAATGTTGGGTTGCGGTTTCgattaggggttacg                                                                                                     >  2:321415/1‑238 (MQ=18)
                                                                                                                                            gaTATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTg                                                                                            <  1:592632/251‑1 (MQ=255)
                                                                                                                                                     ggCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGCAGCGATGGTGGTGGGGGAAGGATTCTTCAGCGCTGAGTGAATCGCCCTTCGGGTCGTTGACTGCGGCAACGCCCTCTCGCTGGCGATCCAGTCGAAACTTGGTCGAAGCTTCTCTTCCTTCCCCGCTTGGGCCGAATATTTAATTGCGGATTTGCTTGAGCATCCCGGGGCTTATGATAGTGAtg                                                                                   >  1:570059/1‑251 (MQ=255)
                                                                                                                                                                                   aTTAGCTCGGCGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTTCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCGCGACGCGAAGGTTAGGAGTGGCTTCTCCTGCTTCCGCGGTTGGGCTGAATATTTGATTGCGGATTCGTTTGGGAGTACCGTGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTagacctatcgacg                                                     >  2:197634/1‑239 (MQ=9)
                                                                                                                                                                                          cGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCGTCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg                                              <  1:69558/251‑1 (MQ=255)
                                                                                                                                                                                                   aGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAGTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCggg                                     >  1:415202/1‑251 (MQ=255)
                                                                                                                                                                                                                         cGATTGTGGTGGGGGAAGGATTATTTAGCGCTGCGCGCGTCGCCCGTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAATATTTGATTGCTGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGctct               <  1:18695/251‑1 (MQ=14)
                                                                                                                                                                                                                         cGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGATCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGctct               <  1:613430/251‑1 (MQ=255)
                                                                                                                                                                                                                              gtggtggGGGAAGGGTTTGTCAGCGCGGCGCGCTTCTACGTGCGGGGTGTTGGCTGCGTCGACGCTCTCTCTCTGGCGGTCAAGTCGAAAGGGGGGGGAAGCGTCGCATCCGTCCCCGCTGGGGCAGAGGATGTGATTGGGGAGGCGTATGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCTTTGCCTGCGGCAACGCTCTCTCGc          <  2:390476/251‑1 (MQ=9)
                                                                                                                                                                                                                                     gggAAGGATTATTCAGCGCTGGGCGCTTCGCCCGTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCt   <  1:308542/251‑1 (MQ=255)
                                                                                                                                                                                                                                      ggAAGGATTATTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGTGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTc  <  1:147058/251‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
GACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTC  >  NC_000913/1286490‑1286970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑