Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,663	C→T	S533F (TCT→TTT)	dhaR →	DNA‑binding transcriptional dual regulator DhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,663	0	C	T	100.0%	169.6 / NA	52	S533F (TCT→TTT)	dhaR	DNA‑binding transcriptional dual regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/25);  total (27/25)

GTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCT  >  NC_000913/1252420‑1252901                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               gTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGc                                                                                                                                                                                                                                          >  1:373319/1‑250 (MQ=255)    tAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGATGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATgg                                                                                                                                                                                                                                      >  2:450418/1‑251 (MQ=255)            aTTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCa                                                                                                                                                                                                                               >  1:143313/1‑250 (MQ=255)                 tGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTCGAACAAAATCGTTTTAGTCGCCAGATGTATTACGCGCTGCATGCATTTGAAATTACCATACCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGACGCTGGTGACTAACAAATTACGCGGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGAGCATGGCCAGGCAACGAtt                                                                                                                                                                                                                         >  1:574988/1‑251 (MQ=255)                  gTCAACGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGCTGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGAttt                                                                                                                                                                                                                        >  1:188761/1‑251 (MQ=255)                  gTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGAttt                                                                                                                                                                                                                        >  1:30981/1‑251 (MQ=255)                   tCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGAtttt                                                                                                                                                                                                                       >  1:366058/1‑251 (MQ=255)                       aGTGATTGCACCAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTCCCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAAc                                                                                                                                                                                                                   <  1:222858/251‑1 (MQ=255)                          gATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACttt                                                                                                                                                                                                                >  1:542165/1‑251 (MQ=255)                           aTTGCAACAACGACCGCGGCCCTCGCAAGGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACttt                                                                                                                                                                                                                <  1:463599/250‑1 (MQ=255)                              gCAACAACGACCGCGGACCTCGCAATGCGGGTGGAACAAAATCGTTTTAGTCGCTAGCTGTATTACGCGCTGCATGCTTTTGAAATTTCCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAg                                                                                                                                                                                                            <  1:105688/251‑1 (MQ=255)                               caacaaCGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGc                                                                                                                                                                                                           >  1:157479/1‑251 (MQ=255)                                aacaacGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGCTGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTCCAGCg                                                                                                                                                                                                          >  2:146910/1‑251 (MQ=255)                                      gACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGGGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACACCTTCATCg                                                                                                                                                                                                    >  2:216458/1‑251 (MQ=255)                                         cgcgGACCCCGCACTGGTGGTGAAACAAAATCGTTTTAGTCGCCAGCTGTGTTACGCGCTGCAGGAATTTGAAATTCCCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCgaga                                                                                                                                                                                                 <  2:350714/251‑1 (MQ=255)                                           gggaccgtgcatggctgtggaaataaaaTCTTTTTGGTCGCCAGTTGTATGCGGCTCTGCTGGCATTTGAAATTACCATCACGCCTCTGCGTATGCTGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCGACGCGGCTGAAGAGTTATGACGATGCCTTCGCTCGCCTGGTTTTTTTTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAAt                                                                                                                                                                                               <  1:407570/227‑1 (MQ=255)                                               ccccgcagtccggggggAAAAAAAGCGTTTTAGGCGCCATCTGTATTTCGCGCTGCATCAATTGGAAATTCCCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGATGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACTATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACTATTTTGAACTTTACAGCGTCATTGAGAATCTTg                                                                                                                                                                                           <  2:105721/236‑1 (MQ=255)                                                cTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGc                                                                                                                                                                                          >  2:246343/1‑251 (MQ=255)                                                     aaTGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTg                                                                                                                                                                                      >  1:539178/1‑250 (MQ=255)                                                            gtggAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTg                                                                                                                                                                               >  2:176444/1‑250 (MQ=255)                                                                           tttggTCGCCAGCTGTGTTACGCGCTTCTTGCATTTTAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTc                                                                                                                                                                <  1:532437/246‑1 (MQ=255)                                                                               gTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTCCCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTc                                                                                                                                                           <  1:246343/251‑1 (MQ=255)                                                                                 cGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGGAGTGGTAACGGGCGCATTCGCGTc                                                                                                                                                           >  2:511329/1‑249 (MQ=255)                                                                                  gCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCAt                                                                                                                                                                  >  2:203179/1‑241 (MQ=255)                                                                                  gCCAGCTGTATTACGCGCGGCATGCATTGGAAATTCCCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGGAGTGATAACGGTCGCAt                                                                                                                                                                  <  1:203179/241‑1 (MQ=255)                                                                                                          catTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTAcc                                                                                                                                <  1:424328/251‑1 (MQ=255)                                                                                                           attggAAATTCCCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCGCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCGGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCg                                                                                                                               <  2:277852/247‑1 (MQ=255)                                                                                                                          cccGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAgat                                                                                                                 <  2:447760/250‑1 (MQ=255)                                                                                                                                     gTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAAc                                                                                                                                                                                                                   >  2:248953/1‑141 (MQ=255)                                                                                                                                     gTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAAc                                                                                                                                                                                                                   <  1:248953/141‑1 (MQ=255)                                                                                                                                          cggcggGGCAGCATTCCGGCGCTGGTTAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTTGTTTTTTGTGCATTGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGACTAGTCATAACGGGCGCATTCGCGTCAGTGATTTGCCGGaa                                                                                                                                            <  2:520918/207‑1 (MQ=255)                                                                                                                                          cggcgTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCg                                                                                                <  2:539178/251‑1 (MQ=255)                                                                                                                                          cggcgTGGCAGCATTCCGGCGATGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCggca                                                                                                                                            >  1:520918/1‑205 (MQ=255)                                                                                                                                              gTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTGGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTGTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGGAGTGAGAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCtt                                                                                            <  1:616382/251‑1 (MQ=255)                                                                                                                                                      aTTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTccacc                                                                                      >  2:51566/1‑249 (MQ=255)                                                                                                                                                        tCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGt                                                                                   <  2:373319/250‑1 (MQ=255)                                                                                                                                                            gcgcTGGTGAATAACACATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTc                                                                              >  1:133589/1‑251 (MQ=255)                                                                                                                                                                 ggTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCAtt                                                                           <  1:31061/249‑1 (MQ=255)                                                                                                                                                                            aatgaCGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGTCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGGCATCGAGAAGCTAGCTCTGAGTAGTGATACCGGGCGCATACGCGTCAGGGATTTACCGAAACATCTCTTTACCGAGGAGGCGACAAAGAAGGTCAGCGCCACCCGCCTTTCCTCCAGTCTTTCATTTGCGGTAGGTGaa                                                              <  2:62424/247‑1 (MQ=255)                                                                                                                                                                               tACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGaaaa                                                            >  2:10881/1‑250 (MQ=255)                                                                                                                                                                               tACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATCACGATGCCCTCGCTCGCCTGGTTGTTTGTGAATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTATGAGTAGTGATAACGGGCGCATTCGCGTCAGTGCTTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTACACCAGTCTGTCATTTGCGGAAGTTGaaaa                                                            >  1:50390/1‑250 (MQ=255)                                                                                                                                                                                aCGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGaaaaa                                                           <  1:245465/250‑1 (MQ=255)                                                                                                                                                                                    aattttgcaaaaCGCTTCTCTACGCGGCTGAAAATTGATGACGAGTCCCTCGCTCGCCTGGTTTTTTGTGAATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTTCCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGTAAGTTGAAAAAGAGGc                                                      <  2:30981/243‑1 (MQ=255)                                                                                                                                                                                     gTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTGTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCa                                                     <  1:309662/251‑1 (MQ=255)                                                                                                                                                                                                       ctACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCCTTTGCGGCAGTTGAAAAAGAGGCAATTATTAACGCCGccc                                     >  2:447644/1‑249 (MQ=255)                                                                                                                                                                                                          cgcgGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTc                                >  1:220875/1‑251 (MQ=255)                                                                                                                                                                                                                           gaCGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCa               <  1:366666/251‑1 (MQ=255)                                                                                                                                                                                                                              gATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTccgg             >  1:383559/1‑247 (MQ=255)                                                                                                                                                                                                                                    ctcgctcgCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATATTGCTCTGAGTAGTGAAAACGGGAGCATCCGCGTAAGTGCTTCGCCGGAACATCTGCTTACCGAGCAGGCGACAGATGATGTCAGCGCCACACGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTAGCACTCAGGAAATGTc      >  1:562002/1‑251 (MQ=255)                                                                                                                                                                                                                                        ctcgcCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCCTTCAGGGAATGGCGGCt  >  1:79271/1‑251 (MQ=255)                                                                                                                                                                                                                                          cgcCTGGTTTTTTGTGCATGGCCAGGCAATGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAATGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCt  <  2:137064/249‑1 (MQ=255)                                                                                                                                                                                                                                          cgcCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCt  <  1:146910/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               GTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCT  >  NC_000913/1252420‑1252901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑