Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,244,908	C→T	L61L (CTG→TTG)	ymgE →	PF04226 family protein YmgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,244,908	0	C	T	100.0%	100.1 / NA	35	L61L (CTG→TTG)	ymgE	PF04226 family protein YmgE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/13);  total (22/13)

GCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATCTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTT  >  NC_000913/1244663‑1245153                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        gCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCTTGAACATGGTAATTATTGCCTGGATTATTTTTGTCCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATCCGCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGcac                                                                                                                                                                                                                                                    <  1:356782/251‑1 (MQ=255)                   taTGTAAGCATTGCTTGCGTCCGAATCATCGAAACGAGGGCCTGAACATGAGGATTATTGCCTGGATTAGTTTTGACCGGTTAGCCGGCATTTACGCCAAGCGAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATGCTCGGGATAGTCGGTGCGGTGGTTGGCGGCTGGTTGACGACCATGTTTGGCATTGGCGGGTCCATCAGTTGGTTTAATTGGCACAGCCCC‑CTGGTCGCgg‑t                                                                                                                                                                                                                                 <  2:37200/249‑1 (MQ=255)                   gATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCgggg                                                                                                                                                                                                                                 >  1:587139/1‑249 (MQ=255)                    aTGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGTCCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGAGTTTTCCTGACCTGTATTCGCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTGGCACAGCTTC‑CTGGTGGCgg‑tg                                                                                                                                                                                                                                <  2:239922/250‑1 (MQ=255)                      gaaaGCATTTATTGCGTCCGATTCATCGAAACGAGGGCCTGACCATGGGACTTATTGCCTGGATTATTTTTGACCTGCTAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCCCCCTCAGGGGGTTTAATTTGCCAAGCTTC‑CTGGGGGCGg‑tggtg                                                                                                                                                                                                                             >  2:589042/3‑251 (MQ=255)                      gTAAGCATTTCTTGCGTCCGATTCAACGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCgg‑tggt                                                                                                                                                                                                                              <  2:20934/250‑1 (MQ=255)                                cTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGGGGGAGCTAttt                                                                                                                                                                                                                   >  1:626677/1‑250 (MQ=255)                                     gTCCGATTCATCGAAACGACGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGCTGGTGGTGGATTTTTCCTGACCTGTATTCTAGGGCTAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTTATTTTCACAGATTC‑CTGTTGGCGG‑GGGGGGGCGCTATTATCGtt                                                                                                                                                                                                              >  2:138188/1‑251 (MQ=255)                                                   aaCGAGGGCCTGAACATGGGAATTATTGCATGGATGATTTTTGACCTGATAGCCGGCATTATCTCAAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGGGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCTGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCg                                                                                                                                                                                                <  1:404681/251‑1 (MQ=255)                                                       aGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGGGCTATTCTCGTTCTGGGCATATTTCGcctc                                                                                                                                                                                            >  1:581681/1‑251 (MQ=255)                                                              gAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGGCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGGGCTATTCTCGTTTTGGGCCTATTCCGCCTCCTGCgcg                                                                                                                                                                                     >  1:265690/1‑249 (MQ=255)                                                                    gtgAATTATTGCCTGGATTATTTGTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGGCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAAt                                                                                                                                                                               <  2:364735/249‑1 (MQ=255)                                                                      gAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATa                                                                                                                                                                              >  1:420057/1‑250 (MQ=255)                                                                       aaTTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCAAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCACTCAGGGGTTTTAATTTGCATAGCTTCTATGG‑GGCGG‑TGGTAGGAGCTATTATCGTTCTGGGCCTATTCCGCCACCTGCGAAGGGAATaa                                                                                                                                                                             >  2:608192/1‑250 (MQ=255)                                                                                             gACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGTAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCg                                                                                                                                                       <  1:589042/250‑1 (MQ=255)                                                                                                cTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGGGGGGGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGGAGAGAATAAGATTTTCATCAGGCggtatgcggta                                                                                                                                                    >  2:89733/1‑241 (MQ=255)                                                                                                cTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGGTTTAATTTGCCCAGCTTC‑CTGGTGGCGG‑GGGGGGGGGCTATTCTCGTTTTGGGCATATTCCGCCTCCTGCGGAGAGAATAAGATTTTCATACGGCGGGTAGCGATAc                                                                                                                                                   >  1:60745/1‑251 (MQ=255)                                                                                                       ccGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGcc                                                                                                                                            >  1:142529/1‑251 (MQ=255)                                                                                                        cGGCATTATCGCCAAGCTAATCATACCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGCCCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGGGGCGG‑GGGGGGGAGCTATTCTCTGTCTGGGCCTTTTCCGCCTCCCGCGGAGGGAAAAAGATTTTTATCAAGCGGATaacggatcacaaggcg                                                                                                                                           >  2:621842/1‑236 (MQ=255)                                                                                                         ggCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGGGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGGGTGG‑GGGGGCTAATCTCGTTCTTGGCCTATTCCGCCTCCTGCGGAGAGAATAAGATTTTTATAAGGCGGGTAGCGCTACAACTGCCGc                                                                                                                                          >  2:35888/1‑251 (MQ=255)                                                                                                             ttATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATc                                                                                                                                      >  1:8811/1‑251 (MQ=255)                                                                                                               aTCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGTATAGCGATACAGATGCCGCTATcc                                                                                                                                     <  1:363947/250‑1 (MQ=255)                                                                                                                   ccAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCCCAGCTTC‑CTGGTGGCGG‑TGGGGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCttt                                                                                                                                >  1:68384/1‑251 (MQ=255)                                                                                                                   ccAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCttt                                                                                                                                <  2:626677/251‑1 (MQ=255)                                                                                                                                             ggtggtggATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGGTTGTCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGGGGCGG‑TGGGGGGAGCTATTCTCGGTCTGGGCATATTCCGGCTCCTGCGAAGAGAATAAGATTTTCATAAGTCGGATAGGGATACAGATGCCGCTATCCGCTTTCCAATCAGAACGTGTACTTGACAccc                                                                                                      >  1:3978/1‑250 (MQ=255)                                                                                                                                                          tCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTTCGCCTCCTGCGAAGAGAATAAGATTTTCATAAg                                                                                                                                                                 >  1:411617/1‑179 (MQ=255)                                                                                                                                                          tCCTGACCTGTATTCTCGGGATAGGCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTGCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTGTCATAAg                                                                                                                                                                 <  2:411617/179‑1 (MQ=255)                                                                                                                                                                       tctcGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGTCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TTGTGGGAGCTATTCTCGGTCTTGGCATATTCCGCCTCCTGCGAag                                                                                                                                                                                    <  2:621258/147‑1 (MQ=255)                                                                                                                                                                       tctcGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑GGGTGGGAGCTATTATTGTTCTGGGCATATTCCGCCTCCTGCGAag                                                                                                                                                                                    >  1:621258/1‑147 (MQ=255)                                                                                                                                                                                    ggtgcggtgGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCCCCATCCGTGGTTTTCATTTGCACAGCTTC‑CTGGTGGCGG‑TGGAGGGAGCTATTTTCGTTCTGGGCCTATTCCGACTCCTGCGGAGAGAATAAGATTTTCATCAGGCGGATAGCGGTACAGATGCCGCTTTCCGCTTTCACATAAGAACGTATACCCGACACCCGCTTTACGGGGGAAAAAACTCAATATCGAACTTCGattt                                                               >  2:80128/1‑249 (MQ=255)                                                                                                                                                                                                ggcggcTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCCCATCAAAACGGATACTCGACACCTGCTTTACGGGTGAAAAAAATCAAAATAGCACTTCGATATCCGGTAAAGTCg                                                   >  2:391430/1‑251 (MQ=255)                                                                                                                                                                                                       ggctggcGACCATGTTTGGCATTGGCGGCTCAATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGGGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTCTCCGCTTTTACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGGCGGTTCCCg                                            >  2:67958/1‑251 (MQ=255)                                                                                                                                                                                                                 cATGTTTTGCAGTGGCGGCTCCATCAGTGGTTTTAATTTGCTCATCTTC‑CTGGTGGCGG‑TTGTGTGAGCTAGTCTCCTTCTGGGCATATTCCGCCTCTTTCGAAGAGAATATGATTTTCATAAGGCGGATATCGATACAGATGCCGCTATCCGCTTTCACATCAGAACTTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCTCTTCGATATCCGATAAAGTCGGTGCCCGGTaccaacaa                                  <  2:579358/251‑1 (MQ=255)                                                                                                                                                                                                                      ttGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGa                              <  1:35888/250‑1 (MQ=255)                                                                                                                                                                                                                                                 ttAATTTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCgttgtt  >  2:215986/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        GCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATCTGCACAGCTTC‑CTGGTGGCGG‑TGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTT  >  NC_000913/1244663‑1245153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑