Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,234,947	A→G	K4E (AAG→GAG)	fadR →	DNA‑binding transcriptional dual regulator FadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,234,947	0	A	G	100.0%	108.2 / NA	35	K4E (AAG→GAG)	fadR	DNA‑binding transcriptional dual regulator FadR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/19);  total (16/19)

ATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTG  >  NC_000913/1234710‑1235187                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 aTCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAAGCGCAAAGccc                                                                                                                                                                                                                                     >  1:337657/1‑251 (MQ=255)     ccATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCcgg                                                                                                                                                                                                                                   <  2:530261/249‑1 (MQ=255)                tCCCTTACCTTACAAATAATCAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCgg                                                                                                                                                                                                                       <  1:522523/250‑1 (MQ=255)                 cccgtaccttacAAATAATCAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGGGAGGCAAGTGTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTGTTTCTTCGTTTTGTCTTCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCCCTATGGTCATTGAGGCGCAGAGCCCGGCGGGTTTCGCGGaa                                                                                                                                                                                                                     <  1:587197/247‑1 (MQ=255)                      accttacAAATAATCAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGGCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTGTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGTGTTGCGCGGAAGAGTa                                                                                                                                                                                                                <  1:468497/251‑1 (MQ=255)                          tacAAATAATCAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGACATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTAGCGGAGGGGTACatt                                                                                                                                                                                                            >  2:526282/1‑251 (MQ=255)                                    cAATGATGTTTTGATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACAGTATTGAAAGt                                                                                                                                                                                                   <  2:502712/250‑1 (MQ=255)                                         atgTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGAGCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACGTTTTTTTGCTGTGTTATGGAAATCTCTCTATGGTCATTGAGTCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTgg                                                                                                                                                                                             <  1:286704/251‑1 (MQ=255)                                                     ttAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGCAAATGAAGCCTTGATACCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTCTTGAAAGTATCTGGAATAACCGCTtc                                                                                                                                                                                 >  1:377464/1‑251 (MQ=255)                                                     ttAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAACATGAAGCCTTGATCCCTTTTTCTTATTTTTGTCTGCTATCAGCGTCGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTtc                                                                                                                                                                                 >  1:377473/1‑251 (MQ=255)                                                             aaaGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCgg                                                                                                                                                                         <  2:567647/251‑1 (MQ=255)                                                               aGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCggg                                                                                                                                                                        >  1:138025/1‑250 (MQ=255)                                                                           aggcaggAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTc                                                                                                                                                                                                                           >  1:271434/1‑187 (MQ=255)                                                                           aggcaggAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTc                                                                                                                                                                                                                           <  2:271434/187‑1 (MQ=255)                                                                                                     gTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCcgg                                                                                                                                                                                                                                   <  1:202665/153‑1 (MQ=255)                                                                                                     gTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCcgg                                                                                                                                                                                                                                   >  2:202665/1‑153 (MQ=255)                                                                                                                agagATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGGTTAGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCCGAACGTGACATTTCAGACTTAATTGGCgaaaatcg                                                                                                                      >  2:30684/1‑244 (MQ=255)                                                                                                                      gCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGtacta                                                                                                                 <  1:443735/250‑1 (MQ=255)                                                                                                                               gAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTg                                                                                                        <  1:423865/250‑1 (MQ=255)                                                                                                                                      tGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCATCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGtt                                                                                                 >  1:602502/1‑250 (MQ=255)                                                                                                                                          cccTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAg                                                                                             <  1:30684/250‑1 (MQ=255)                                                                                                                                                ttcttcTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGCCTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTgg                                                                                      >  2:476581/1‑251 (MQ=255)                                                                                                                                                                    cAGCGTAGTTATCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTGCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGTATTAATTGGCGTAACGCGTTCTACGTTACGTGAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGAc                                                                   <  1:572617/250‑1 (MQ=255)                                                                                                                                                                         tAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAACGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTATGGCACGAGATGGCTGGTTGACCATTc                                                              >  1:273715/1‑250 (MQ=255)                                                                                                                                                                           gTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATGGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGACCATTCAAc                                                           <  1:284424/251‑1 (MQ=255)                                                                                                                                                                                 ccTCTGGTATGATGAGTCCAACATCGGATTGGTGTGTTAAGGAAATCTCACGATGGTCATTGAGGCGCAAAGCACGGCGGGTCTCGCGGAAGAGTACATGATTGAAAGTATCTGGAATAACCGCTTACCTCCCGGCACTATTTTGCCCGCATAACGTGAACTTGCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCa                                                     <  1:607683/251‑1 (MQ=255)                                                                                                                                                                                         atgatgAGTCCAAATTTGTTTTCCTGTGTTATGGCAATCTCCCTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACCTTATTGAAAGTCTCTGGAATACCCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACATGCACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTGGCACGAGATTGCTGGTTGACCATTCAACCTGGCACGCcgacg                                             >  2:262028/1‑251 (MQ=255)                                                                                                                                                                                          tgatgaGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCcgacg                                             >  1:338935/1‑250 (MQ=255)                                                                                                                                                                                                    aaCTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGaataa                                  <  2:224459/251‑1 (MQ=255)                                                                                                                                                                                                         tGTTTTGCTGTGTTATGGAAATCTCCCTATGGTCATTGAGGCTCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGTCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGGCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTc                              <  2:377473/250‑1 (MQ=255)                                                                                                                                                                                                         ggTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTAGCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACTTTACGTGAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTTAATAATTTc                              <  2:377464/249‑1 (MQ=255)                                                                                                                                                                                                                  gtgtTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGCCCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTCTGGGAAACtt                    >  2:541506/1‑251 (MQ=255)                                                                                                                                                                                                                  gtgtTATGGAAATCTCACTATGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCATCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGAGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTTTGGGAAACtt                    >  1:626659/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTGGTCATTGAGGGTCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTGCCCTCCCGGGACTAGTTTGCCCGCAGAACGTGAACGTTCAGAATTAATGGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTGGCACGGGATGGCTGGTGGACCATTCAACATGGCAAGCCGACGAAGGTGTATAATTGCTGGGAATCGTCCGGTTTAAATAACCTTg  <  2:620852/250‑1 (MQ=255)                                                                                                                                                                                                                                     aTGGTCATTGAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTg  >  1:376404/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 ATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACGTGAACTTTCAGAATTAATTGGCGTAACGCGTACTACGTTACGTGAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTG  >  NC_000913/1234710‑1235187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑