| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,227,656 | G→A | intergenic (‑184/‑25) | pliG ← / → ycgL | inhibitor of g‑type lysozyme/PF05166 family protein YcgL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,227,656 | 0 | G | A | 95.7% | 60.9 / ‑6.6 | 23 | intergenic (‑184/‑25) | pliG/ycgL | inhibitor of g‑type lysozyme/PF05166 family protein YcgL |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (8/14); minor base T (0/1); total (8/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
GCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGT > NC_000913/1227413‑1227892 | gcgcGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCAGTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAGCCGTAGAAATCTACAGCTGGCTTGCTTGCGACGCGGGt < 2:422765/248‑1 (MQ=255) gCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTAt < 2:574159/251‑1 (MQ=255) tCATTGGTAGTCTCCTGTTCAGAATGAGGACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGTGGATTACTTATTAAGGGGGCATGTTCTCAACATACCGTAGAAATCTACAGCTGGCTGGCTTTTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGtt < 1:262682/250‑1 (MQ=255) ccTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACGCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGTCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATc < 1:104319/251‑1 (MQ=255) aaTGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGG‑ATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCaa < 2:20126/251‑1 (MQ=255) gAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGATTAAAAGTATGGTTTGTGTGAGTTAGCGAAGCAGCAAGc < 2:413927/251‑1 (MQ=255) aGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGATTTCCTACGTTCGGTAAAATCACGTTGAGGAATTTTTGAAGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCAGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACATGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGAAAGCg > 1:37794/1‑251 (MQ=255) aaatataaCAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCATCAAGCGTGACCAGTCCTATTtat < 2:568232/250‑1 (MQ=255) tataaCAATCACTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGG‑ATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGt > 2:462812/1‑251 (MQ=255) cccTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGt < 2:497830/194‑1 (MQ=255) cccTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGt > 1:497830/1‑194 (MQ=255) cGGGAGAATTCCAGTCGCTTTCCCGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATAGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACg > 2:400549/1‑251 (MQ=255) aTTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTc < 2:471083/251‑1 (MQ=255) gttttCCTGCGTTCGTTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTTATTATAAAGGGGGCATGGACTCAACAACCCGTAGAAATCTACATCTGGATGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAATCCTGGCACCTTAAAGAGTAAAAGTAGGTTTTGTGTGATTTATCGAAGCAGCTATTGTGACTAGTCCTATGTATATGTCGAAAAAAAAGATGATCTTTCGCGTGTTct < 2:204826/249‑2 (MQ=255) aaaTCAAGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAgg > 1:469649/1‑251 (MQ=255) ggCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGcc < 1:141142/251‑1 (MQ=255) tGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTa < 2:178771/250‑1 (MQ=255) ataAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTGAGCGATGATTCTGCCGCt < 2:252219/250‑1 (MQ=255) ggggCAAGGACTAAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGATCGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGAATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAACAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATggg > 2:544741/1‑251 (MQ=255) gggCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGtttt > 2:199148/1‑211 (MQ=255) gggCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGtttt < 1:199148/211‑1 (MQ=255) aaaCCGTAGAAATCTACAGCTGGCTGGCTTGAGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGATTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc > 1:139107/1‑250 (MQ=255) ggctggctTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAATTATGTAGTTTGTGATTTATCGAAGCAGCAAGCGTGACCAGATCTATTTATATGTCTAAAAAAGAGACGATGGTGCGCGGGTTCCTGAGGTACTGATGAAAGGGGTTGGTCTGCCTCAGTTAGCGATGATTCTGCTGCTGGATGTGGGGAAGAAACTGGTCAATGCCGATATTGTAAAGGt < 2:282705/249‑1 (MQ=255) | GCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGT > NC_000913/1227413‑1227892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |