Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,194,922 G→A T10T (ACC→ACT)  rluE ← 23S rRNA pseudouridine(2457) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,194,9220GA97.4% 110.9 / ‑5.3 38T10T (ACC→ACT) rluE23S rRNA pseudouridine(2457) synthase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (15/22);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

TATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCC  >  NC_000913/1194681‑1195168
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      
tATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTc                                                                                                                                                                                                                                                <  1:321652/250‑1 (MQ=255)
     gtccgCACCAGCAACACTTCGCTATCGCGGTTAAGTCGACCTGCTGCATAAACACCCTGAATCGGGATGACTTCTTTTAATGTTTTGCGTCCGGCTTCATCGGCGAACTGCGGAAGCACATTGTAGGGGTTCTTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTAGGCATGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGCTTTCTACAAGCAGTTTTTTGCATGGTATTTTctg                                                                                                                                                                                                                                              >  2:166683/5‑245 (MQ=255)
                 aaCCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCg                                                                                                                                                                                                                              <  2:286698/251‑1 (MQ=255)
                    ccTTCGCTATCGCTGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAAGTGCCGCAt                                                                                                                                                                                                                           <  1:202237/251‑1 (MQ=255)
                         gCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATATGATTTTCAGAGATTATGAATTGCCGCATtatat                                                                                                                                                                                                                      <  2:456640/251‑2 (MQ=255)
                                 ggTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCtaataa                                                                                                                                                                                                              <  2:323279/251‑1 (MQ=255)
                                       ggCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAAGCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCa                                                                                                                                                                                                        <  2:85334/251‑1 (MQ=255)
                                           aCCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCAGCGGTGAACTGCGGAAGAACATCGTAGGGTTTAGTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATGTCTAAAAGAAGTTTTTTGCATAGTATTTTAAGAGATTAGGAATTGCCGCAGTATAGCCTAATAACGCGCATcgt                                                                                                                                                                                                    <  2:65424/251‑3 (MQ=255)
                                                              tGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCAGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAAt                                                                                                                                                                                   >  1:139625/1‑249 (MQ=255)
                                                                       aTGAATTCTTTTCATGTTTTGCGGCCGGCTTCATCGATGAACTGCGGACGAACATGGTAGGGCTTATTGCTCAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGTGATTATGAATTGCTGACTTATAGCCTAATAACGCGCATCTTTCATGAAGGCAAACAATAGGGTAGTAtt                                                                                                                                                                        >  2:43154/1‑251 (MQ=255)
                                                                              attttAATGTTTTGCGTCCGGGTTCCTGGGTAAACAGCGGAAGAATATCGTAAGGGTTATTGAAACGGATCACACGCATGGGCTGGTCATCAGGTTTACGCCTGCTAGAACGTTGGGAGCTGAATCGCTTAACCTGGTGTATTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCATAATAACACGCATCTTTCATGACGGCACACAATAGGGTAGTATTGACAAGc                                                                                                                                                                 >  2:374578/2‑251 (MQ=255)
                                                                                  tAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAAt                                                                                                                                                             >  1:404972/1‑251 (MQ=255)
                                                                                  tAATGTTTTGAGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAAt                                                                                                                                                             >  2:190391/1‑251 (MQ=255)
                                                                                                   cTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACaa                                                                                                                                             >  1:46540/1‑250 (MQ=255)
                                                                                                       aTCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATATCGCGCATCTTTCATGa                                                                                                                                                                                              <  2:378953/197‑1 (MQ=255)
                                                                                                       aTCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGCGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGa                                                                                                                                                                                              >  1:378953/1‑197 (MQ=255)
                                                                                                                     gaagaaCATCGTCGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTAAGCCTGGTAGAACGTTGCGAGCTGACTCGCTTAACCTGGTGATTTCTCAAAGAAGTTTTTTGCATAGTATTTTAAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAACAAATTGCTCTAAAGCATc                                                                                                                          >  2:239574/1‑251 (MQ=255)
                                                                                                                      aagaaCATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAAGTGCTCTAAAGCATcc                                                                                                                         <  2:46540/251‑1 (MQ=255)
                                                                                                                          aCATCGGAGGGTTTATTGAACAGGCTCACACGCGTGGGCTGGTTTTCAGGTTTACGCATGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATTAATTGCAGCATTATAGCCTAATAACGCGCATCTTTCATGACGTCAAACAATCGGGTAGTATTGACAAGCCACTTACAAATCATTAACAAAAAATTGCTCTAAAACATCCGTAt                                                                                                                     >  2:463519/1‑251 (MQ=255)
                                                                                                                           catcgttggagttATTGAACTGGATCACGCGCGTGAGCTGGTTTTCAGGTTTACGGCTGGTAGACCGTTGCGGGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGTTTATGAAGTGACGAATTATAGCCTAATAACGCACATCGTGCATGACTGCAAAGAAGAGGGTAGTATTGACAAGCCAATTCCAAAACATTAACAAAACATGGCTCTAGAGCGTCCATATc                                                                                                                    <  2:188139/240‑1 (MQ=255)
                                                                                                                           cATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATc                                                                                                                    <  1:344723/251‑1 (MQ=255)
                                                                                                                                aaGGGGTTATTGAACCGGATCACACGCGAGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTACAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCACACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGAATCGCAgg                                                                                                               >  2:357779/2‑251 (MQ=255)
                                                                                                                                 aGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGa                                                                                                              <  1:345126/251‑1 (MQ=255)
                                                                                                                                  gggTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGAc                                                                                                             >  1:365049/1‑251 (MQ=255)
                                                                                                                                        aTTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAAc                                                                                                       <  1:542913/251‑1 (MQ=255)
                                                                                                                                               aGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATg                                                                                                >  1:65418/1‑251 (MQ=255)
                                                                                                                                                    cacaCGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACg                                                                                           <  1:315896/251‑1 (MQ=255)
                                                                                                                                                               gCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCTAGCTGAATCGCTTAACCTGGTTATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCACCGGGGTGGCAGAc                                                                                <  2:21364/251‑1 (MQ=255)
                                                                                                                                                                                    tGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACg                                                                                                                                                                                            >  2:552965/1‑122 (MQ=255)
                                                                                                                                                                                    tGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACg                                                                                                                                                                                            <  1:552965/122‑1 (MQ=255)
                                                                                                                                                                                     ggTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTAGTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCg                                                            <  1:97501/249‑1 (MQ=255)
                                                                                                                                                                                                                aaCCTGGTGAGTTCTAAAAGAAGCTTGTTGCCGAGGATTTTCAGGGATTATGAAATGCCGCATTATAGCCTAATAACGCGCACCTTTCATGCCTGCAAACTATATGGTAGTATTGAGAAGCCAATTACATATCATTAACAAAAAATTGCTCTAAAGCATCCGGAGCGCAGGACGGAAACGCAGAGGCAGCTTGGTGGCAGACAAGCAAACCAGTAGCGCTGGAAGGAGTGTTGAATTGGAAGAAAagtag                                <  2:312633/250‑1 (MQ=255)
                                                                                                                                                                                                                             cTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGcaca                   >  1:338039/1‑250 (MQ=255)
                                                                                                                                                                                                                                 aagaagTGTTTGGCATAGGATTTACAGAGATTATGAAGTGCCGCATTATAGCCTAATAACGCGGAACGTACATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATGAACAAAAAATTGCTCTAAAGCAGCCGGATCGGAGGACGCAAACGCATAGGCAACGTGGTGGCAGACTAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAGGTAAAGTAGTTGTTCCGCCACAAGGCa              <  2:219839/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gaagTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCaag            <  2:80017/251‑1 (MQ=255)
                                                                                                                                                                                                                                          tttGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTTGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGATGTTCCGGCACAAGGCAAGAAGATc      <  2:572269/250‑1 (MQ=255)
                                                                                                                                                                                                                                            tGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGACAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGCATCGCAGGACGAAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGGGAATGGTAAGTACAGTAGTTGATCCGGCACAAGGCAAGAAGATCAcc   >  1:388216/1‑251 (MQ=255)
                                                                                                                                                                                                                                             gCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCAccc  <  1:189494/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      
TATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCC  >  NC_000913/1194681‑1195168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑