Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,176,602	C→T	P392P (CCC→CCT)	lolC →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,176,602	0	C	T	100.0%	158.1 / NA	51	P392P (CCC→CCT)	lolC	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/30);  total (21/30)

CATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTG  >  NC_000913/1176356‑1176846                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     caggatgGTCTTTATGGTGAAAGGGGCCATCGCCGGGATAATCGGGGCGATCCTCGGAGCGGCGCGGGGCGCCCTGCTTGCCAGCCAGTTAAATAAGCTGATGCCGATAATCGGCGGCCTGCTTGAGGGCGCGGCGCGGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATGGCGCTGGTGGCGAGGGCTATCGCGCGGCTGGCTACGCTTTACCCTTCATGGCGCGCGTCCGCCACTCAACctgctg                                                                                                                                                                                                                                                  <  2:287692/248‑1 (MQ=255)            ttATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTa                                                                                                                                                                                                                                          <  1:288417/248‑1 (MQ=255)            ttATGGTGCAAGGGGCCAGCGCCGGGATTAGCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATTCCGATAATCGGTTTCCTGCTTCATGGCGCGGCGCTGCCGGTGGCTATCGACCCTTTACGGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGATTTACCCTTCATGGCGGGCTGCCGCCACTCAACCTGCTGAGGCTTTa                                                                                                                                                                                                                                          <  1:7435/248‑1 (MQ=255)              aTGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGtt                                                                                                                                                                                                                                      >  1:154766/1‑250 (MQ=255)                         ggCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACCCAACCTGCTACGGCTTTACGTTATGAATa                                                                                                                                                                                                                               >  2:223020/1‑246 (MQ=255)                             aGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGGCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGt                                                                                                                                                                                                                      >  2:30454/1‑251 (MQ=255)                                gcCGGGATTATCTGGGCGATCCTCGGAGCGGCGCGTGGCGCCCTGCTTTCCAGCCAGTTAAATAATCTGGTGCCGAGAATCGGCGTCCTGCTTGATGGCGCGGCTCGGCCGGTGGCTATCGTACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGGCTACGCTTTACCCTTCATGACGCGCGGCCGCCACTCAACCTGCTGATGCGTTACGTTATGAATAAGATCCTGTTGc                                                                                                                                                                                                                   <  1:121911/251‑1 (MQ=255)                                   gggATTATCGGTGCGACCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCCTGGCGCGCTGCAGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCATGTTGctat                                                                                                                                                                                                                >  2:117283/1‑248 (MQ=255)                                           cggggCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTGGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATTGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAAc                                                                                                                                                                                                        <  1:552392/247‑1 (MQ=255)                                             gTGCGATCCTCGGAGGGGCGCTTGGCGCCTTGCATGCCAGCCAGTTAAAAAATCTGATGCCGATAATCGGCGTCCTGCATGATGGCGCGTCGCTGCCGGTGGCTAGCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCTATGTCTATCGCGCTGCGGTCGACGCTTTTCCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGGTATGAATAATATCCTGGTGCAATGCGACAACCt                                                                                                                                                                                                      <  2:518167/251‑1 (MQ=255)                                                       cGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGc                                                                                                                                                                                            >  1:152238/1‑251 (MQ=255)                                                       cGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCGGCCGGAGGCTATCGAACCTTTACAGGTCTGTGTTATTGCGCTGGGGGCGGTGGATATCGCACGGCTGTCTACGCTTTACCCGTCAAGGCGCGCTGCCGCCACTGAACGTGCTGAGGCTTTACGGTCTGAATAAGAACATGTTGCAATTCGACAACCTGTGCaaaagc                                                                                                                                                                                            >  1:606617/1‑248 (MQ=255)                                                            gggcgCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCGTGATGGCGCGGCGCTGCCGGTGGCTATCGATCCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCTCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACTTTATGAATAAGATCCTGTTGCAATTCGACAATCGGTGCATACGCTATCa                                                                                                                                                                                       <  2:617134/250‑1 (MQ=255)                                                               cgtatGGCGCCCTGCGTGCCAGCCAGTTAAATAATCGGATGTCGATAAGCGGCGTCCTTCTTTATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTGATTGCGCTGGTGGCGATGGCTCTCGCGCTGCTGTCTGCGCTTTACCCTGCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGa                                                                                                                                                                                    <  2:145229/247‑1 (MQ=255)                                                                gcTTGGTGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGaa                                                                                                                                                                                   <  1:521872/251‑1 (MQ=255)                                                                    ggCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGACTAAGATCCTGTTGCCATGCGACAACCTGTGCAAACGCTATCAGGAAGGc                                                                                                                                                                                >  2:19701/1‑250 (MQ=255)                                                                        cccTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAgtgt                                                                                                                                                                           <  1:505967/251‑1 (MQ=255)                                                                        cccTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAgtgt                                                                                                                                                                           <  2:61172/251‑1 (MQ=255)                                                                         ccTGCTTGCCAGCCAGTTAAATAATCTGATGCCGAGAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGACCCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTGACCCTTCATGGCGCGCGGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGAGCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGtgtg                                                                                                                                                                          <  2:630410/251‑1 (MQ=255)                                                                                                     aTGCCGATAATAGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATAGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGAAAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGtt                                                                                                                                              >  2:292199/1‑251 (MQ=255)                                                                                                        ccGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTACCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAg                                                                                                                                                                                  <  1:616288/212‑1 (MQ=255)                                                                                                        ccGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTACCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAg                                                                                                                                                                                  >  2:616288/1‑212 (MQ=255)                                                                                                                cGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCgg                                                                                                                                    <  1:362467/250‑1 (MQ=255)                                                                                                                    gTCCTGTTTTATGGCGCGGCGCTGCCGGTGGCTATCGAACCTGTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAg                                                                                                                               <  1:223020/251‑1 (MQ=255)                                                                                                                         gtctGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTGACATGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTGCCCTTCATGGCGCGCTGCCGCCACTCATCCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTGGCAATGCGACAACTTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGGCGGCGAAGGTg                                                                                                                            <  2:408834/246‑1 (MQ=255)                                                                                                                              aTGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACGGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGACTAAGATACTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGCAGGAAGTGTGCAACCCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGAGCAatga                                                                                                                      >  1:369380/1‑250 (MQ=255)                                                                                                                                ggcgcggcgcTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATgg                                                                                                                   >  1:71846/1‑251 (MQ=255)                                                                                                                                  cgcggcgcTGCCGGTGGCTATCGAACTTTGACAGGTCATTGTTATTGCGCTGGTGGCGATGGCGATCGCGCTTCTGTCTACGCGTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCg                                                                                                                 <  1:551849/251‑1 (MQ=255)                                                                                                                                            ccGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGATTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGt                                                                                                        >  1:466622/1‑250 (MQ=255)                                                                                                                                             gggtgagtttagAACTTTTACAGGTCATTGTTATTGCGTGGGTGGCGTGGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCAGGGTGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAg                                                                                                      <  2:455935/240‑1 (MQ=255)                                                                                                                                             cGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAg                                                                                                      <  2:6609/251‑1 (MQ=255)                                                                                                                                                 ggCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGctc                                                                                                   <  1:525584/250‑1 (MQ=255)                                                                                                                                                  gCTATCGAAACTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTg                                                                                                 >  2:215214/1‑251 (MQ=255)                                                                                                                                                       cGAACCTTTACAGATCATTGTTATTGCGCTGGTGACGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTCTGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAACCGCTATCAGGAAGGCAGTGTGCACACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGAGAAATGATGGCGATCGTCGGTAGCTCTGGTTcc                                                                                            >  2:578084/1‑251 (MQ=255)                                                                                                                                                                aCAGGTCATTGTTATTGCGCTGGTGGCGAGGGCTATCGCGTTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGt                                                                                   <  1:19701/251‑1 (MQ=255)                                                                                                                                                                  aGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTAc                                                                                 >  2:313504/1‑251 (MQ=255)                                                                                                                                                                               tGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACct                                                                     <  1:562561/250‑1 (MQ=255)                                                                                                                                                                                      gtggCGAGGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCGGCACAATGTCATTTTCAGCGTCTGCGAAGGTGAAATGATGGCGATCGTCGTTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggcg                                                             <  1:392535/251‑1 (MQ=255)                                                                                                                                                                                                  aTCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATAc                                                   <  1:313504/249‑1 (MQ=255)                                                                                                                                                                                                  aTCGCGCTGCTGTCTAAGCTTTACCCTTCATGGCGCGCTGACGACACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGTTACGc                                                 >  2:358025/1‑251 (MQ=255)                                                                                                                                                                                                             gTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGc                                      <  1:238166/251‑1 (MQ=255)                                                                                                                                                                                                              tCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCg                                     >  2:471092/1‑251 (MQ=255)                                                                                                                                                                                                                 ccGCTTTACCCTCCATGGCGCGCTTCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGAtg                                  <  2:558826/250‑1 (MQ=255)                                                                                                                                                                                                                       tccccTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGAGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGAtt                             <  1:415347/248‑1 (MQ=255)                                                                                                                                                                                                                        ccccTTCATGGCGCGGTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGAtttt                           <  2:284723/250‑1 (MQ=255)                                                                                                                                                                                                                        aCCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGAtttt                           >  1:611761/1‑251 (MQ=255)                                                                                                                                                                                                                         cccTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGAttttt                          <  1:80200/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTCCGTTATGACTAAGCTCCTGATGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATAATCCAACCTCAGGCGATGTGATTTTTAATGGTcc                  >  2:102245/1‑250 (MQ=255)                                                                                                                                                                                                                                 ggCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATTAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGGCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGGGCCGTGCTGCACCTGCTGGGCGGTCTGGATACGCCGACGTCCGTCGAGGTGGGTGTTAATGGTc                   <  2:322931/250‑1 (MQ=255)                                                                                                                                                                                                                                        tGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATg           >  1:241630/1‑251 (MQ=255)                                                                                                                                                                                                                                          ccgccACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGctc                                                                                                   <  2:453927/161‑1 (MQ=255)                                                                                                                                                                                                                                                 tCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTg  >  1:408061/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTG  >  NC_000913/1176356‑1176846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑