Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,171,417	G→A	L850L (CTG→TTG)	mfd ←	transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,171,417	0	G	A	100.0%	185.5 / NA	58	L850L (CTG→TTG)	mfd	transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/31);  total (27/31)

TTCAAGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACG  >  NC_000913/1171172‑1171654                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              ttaaagaagattttGTGCATCGGTAGTCATCGCTTTTGGATGCGTGGCCAGCACCCATGCATATGCCTGATGATGCGAACGTCCGACGAGACCGCGTCACTGGTGCAGCTGCGCCAGACCGAGGGGATCCGCGAGTTCAATGATAATAGTGTTGGATGTCGGGCTGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGAGCGTTGACACGTTGATGCTGTAAATCCTTCATCACCCGTTCCAATtccc                                                                                                                                                                                                                                          >  2:376137/11‑249 (MQ=255)  tCAAGACGTTTTTGAGCATCGGTAGTCATCTCTTTTGGCTTCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGCAGGGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCCTCCCCCGTTCCAATtcgcc                                                                                                                                                                                                                                         >  2:488441/1‑250 (MQ=255)     aGACGTTTCTGTGCATAGGTCGTCCTCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGCCCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGCTGATGGAAATCATTCATCACCCGTTCCAATtcgcgctc                                                                                                                                                                                                                                      >  2:294168/1‑251 (MQ=255)            tttGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCAt                                                                                                                                                                                                                                <  1:216801/250‑1 (MQ=255)             ttGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCt                                                                                                                                                                                                                              >  1:227828/1‑251 (MQ=255)               gtgCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGc                                                                                                                                                                                                                            <  2:440157/251‑1 (MQ=255)               gtgCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGc                                                                                                                                                                                                                            <  1:615957/251‑1 (MQ=255)                 gCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGcc                                                                                                                                                                                                                           <  1:394885/250‑1 (MQ=255)                            cATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATgg                                                                                                                                                                                                               >  1:159342/1‑251 (MQ=255)                              tCGCTTTTGGATGCGGTGTAAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCg                                                                                                                                                                                                             >  2:68943/1‑251 (MQ=255)                                            ggTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTgg                                                                                                                                                                                               >  1:138129/1‑251 (MQ=255)                                               gtCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCAc                                                                                                                                                                                            <  2:194978/251‑1 (MQ=255)                                                 cAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCAcc                                                                                                                                                                                           <  2:295703/250‑1 (MQ=255)                                                 cAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCa                                                                                                                                                                                          <  1:518661/251‑1 (MQ=255)                                                     aaCCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTc                                                                                                                                                                                      >  2:178481/1‑251 (MQ=255)                                                       ccATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTg                                                                                                                                                                                    >  2:293286/1‑251 (MQ=255)                                                          tGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGACGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGGTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGccc                                                                                                                                                                                 >  2:147198/1‑250 (MQ=255)                                                           gCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAg                                                                                                                                                                                <  1:219278/251‑1 (MQ=255)                                                               atGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTTAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATtcgc                                                                                                                                                                                                                                          <  1:139132/189‑1 (MQ=255)                                                               atGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGCTGGAAATCATTCATCACCCGTTCCAATtcgc                                                                                                                                                                                                                                          >  2:139132/1‑189 (MQ=255)                                                                   cTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGAATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGGTCTGCCAGCCGTTcgg                                                                                                                                                                        >  1:182561/1‑251 (MQ=255)                                                                     gatgatGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTcggc                                                                                                                                                                       <  1:251967/250‑1 (MQ=255)                                                                     gatgatGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGGCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTcggcg                                                                                                                                                                      <  2:446136/251‑1 (MQ=255)                                                                      atgatgCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCTCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTcggcgg                                                                                                                                                                     <  2:106352/251‑1 (MQ=255)                                                                      atgatgCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCATTCAATGATAATAGTGTTGGCTGTCGGGATGGCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTcggcgg                                                                                                                                                                     <  2:427615/251‑1 (MQ=255)                                                                          tgCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCttt                                                                                                                                                                 >  1:16253/1‑251 (MQ=255)                                                                             gAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTg                                                                                                                                                              >  1:256815/1‑251 (MQ=255)                                                                                cGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGAGCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAAt                                                                                                                                                           <  1:273119/251‑1 (MQ=255)                                                                                               cgTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGTTTTCTGAATGTTTTCCACATCAtt                                                                                                                                            <  2:134732/251‑1 (MQ=255)                                                                                               cgTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAAGGTTTTCCACATCAtt                                                                                                                                            <  1:474135/251‑1 (MQ=255)                                                                                                       gTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGat                                                                                                                                    <  1:91420/251‑1 (MQ=255)                                                                                                          cAGCTGCGCCATACCGAAGTGAGCCTCGCGGTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGGGTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCAGCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCTATGGTGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTTTAGAGataat                                                                                                                                 <  1:548015/251‑1 (MQ=255)                                                                                                            gCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGAtaataa                                                                                                                               <  1:290420/251‑1 (MQ=255)                                                                                                                  ccAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTGTTCCACATCATTGTAGAGATAATAAACCTGt                                                                                                                         <  2:227828/251‑1 (MQ=255)                                                                                                                      aCCGAAGTGATCCGCGCGTTCAATGATAATAGTGTGGGCTGCCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGCATGTTTTCCACATCATTGTAGAGATAATAAACCTGtcctc                                                                                                                     >  2:395363/1‑251 (MQ=255)                                                                                                                       ccGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGtcctcc                                                                                                                    <  2:573600/251‑1 (MQ=255)                                                                                                                       ccGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGtcctcc                                                                                                                    >  1:329502/1‑251 (MQ=255)                                                                                                                       ccGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCGCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGtcctcc                                                                                                                    <  1:68943/251‑1 (MQ=255)                                                                                                                         gAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCcgc                                                                                                                  <  1:600864/251‑1 (MQ=255)                                                                                                                                   gcgcgTTCAATGATAATAGGGTTTGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCTATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTc                                                                                                        <  1:320895/251‑1 (MQ=255)                                                                                                                                    cgcgTTCAATGATAATAGTGTTGGCTGTCGGGATGTCTATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTTCCACCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTc                                                                                                        >  2:165599/1‑250 (MQ=255)                                                                                                                                          cAATGAGAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAAGTGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAg                                                                                                  <  2:54261/250‑1 (MQ=255)                                                                                                                                           aaTGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGa                                                                                                <  1:272402/251‑1 (MQ=255)                                                                                                                                                          ggCTGTCTGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGAACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGacca                                                                                 <  2:221023/251‑1 (MQ=255)                                                                                                                                                            cTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATAGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGaccacc                                                                               >  1:364984/1‑251 (MQ=255)                                                                                                                                                                 gggATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCt                                                                          >  1:101845/1‑251 (MQ=255)                                                                                                                                                                                    cGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATc                                                                                              <  1:500886/212‑1 (MQ=255)                                                                                                                                                                                    cGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATc                                                                                              >  2:500886/1‑212 (MQ=255)                                                                                                                                                                                       tAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGtt                                                    >  2:573009/1‑251 (MQ=255)                                                                                                                                                                                              tGTACAAACCAGCACGTTGAAACGTTGTTGATGGAAATCATTCATCACCCGTTCCAAGTCGCGCTCGCGCAGCTGCCCGTGACCGATGGCGATCCGCGCTTCTTGCACCAGATCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACt                                              <  1:300000/250‑1 (MQ=255)                                                                                                                                                                                                    aaCCAGCCCGTTGAAACGTTGATGATGGAAATAATTCATCACCCGTTACAATTCGCGCTCGCGCATCTGCCCGTGACCGCTGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGCTTTCCACATCCTTGTAGGGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCCGGATCGCCCCCCGGACCACCATGCTGTCATACTCACGGACAAAGGGTTTAACTGCCAgac                                       >  2:73962/1‑251 (MQ=255)                                                                                                                                                                                                           aCGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGAGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCATTCGGCGGCTTTCTGCATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGAcgggc                                >  1:564895/1‑251 (MQ=255)                                                                                                                                                                                                              ttGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCCGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGAcggtcggg                             >  1:39311/1‑251 (MQ=255)                                                                                                                                                                                                                      ttGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCACGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAAATGCCAGACGACGGGCGGGCGGCGTgg                     >  2:601824/1‑251 (MQ=255)                                                                                                                                                                                                                           gatgGAAATCATTCTTCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACGCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGAt                 <  2:329224/250‑1 (MQ=255)                                                                                                                                                                                                                                aaaTCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGTCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCg            >  2:462330/1‑250 (MQ=255)                                                                                                                                                                                                                                       tcatcaCCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTcc    >  2:321273/1‑250 (MQ=255)                                                                                                                                                                                                                                         atcccccGTGCCAATTCGCGCTCGCGCATCTGCCCGGGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCTAAATTTCACGCAGGATCGCCGCCCGGACCACCATGCTGTCATACTCACTGACAAAGGTGTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACg  <  2:507744/247‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              TTCAAGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACG  >  NC_000913/1171172‑1171654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑