Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,161,403	G→A	A50V (GCC→GTC)	fhuE ←	ferric coprogen/ferric rhodotorulic acid outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,161,403	0	G	A	96.4%	83.6 / ‑6.4	28	A50V (GCC→GTC)	fhuE	ferric coprogen/ferric rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (11/16);  minor base T (0/1);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATTT  >  NC_000913/1161187‑1161633                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       gAATAATAAAGAGCACGCTCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGc                                                                                                                                                                                                      >  2:290851/1‑251 (MQ=255)      ataaAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTg                                                                                                                                                                                                 <  1:380566/251‑1 (MQ=255)        aaaGAGCACGATCGGAATCCGCCTGACTTTTGCTGTGCCCCAGCGTGTTTTCCATCCCTTCGCCCAGCGTGTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGATGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCGGTAATCATTTTCGCCATCATCTGGAGCTGTGTCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTgca                                                                                                                                                                                               <  1:557553/251‑1 (MQ=255)        aaaGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTgc                                                                                                                                                                                                >  1:551197/1‑250 (MQ=255)        aaaGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTGTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTGTGGTACCCGCAGAGGTAGACGTGACGCTGTAATCATTTTCGCCAGCATCTGGAGCTGTGACTGATCCCTCAACAAGGACCGTTGCTTCAGTGGCTGGTgca                                                                                                                                                                                               <  2:299881/251‑1 (MQ=255)               aCGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAg                                                                                                                                                                                        <  1:75075/251‑1 (MQ=255)                    cGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGc                                                                                                                                                                                    >  1:224497/1‑250 (MQ=255)                    cGGAATCCGCCTGACTGTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCAGCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCTCGTTGAGGCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTGTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGc                                                                                                                                                                                    <  1:91276/250‑1 (MQ=255)                                               ccAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTACATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCa                                                                                                                                                         >  1:551208/1‑250 (MQ=255)                                                       ttttCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCa                                                                                                                                                  >  1:168454/1‑249 (MQ=255)                                                                            gTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCg                                                                                                                                                                                      <  2:240754/192‑1 (MQ=255)                                                                            gTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCg                                                                                                                                                                                      >  1:240754/1‑192 (MQ=255)                                                                            gTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGc                                                                                                                           >  1:463820/1‑251 (MQ=255)                                                                                gTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGa                                                                                                                       <  2:537884/251‑1 (MQ=255)                                                                                                          tgGCTACCAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGAAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATAttagatcgggagggcgtcgggt                                                                                               >  2:569707/1‑229 (MQ=255)                                                                                                          tgGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATAtt                                                                                                                   <  1:569707/229‑1 (MQ=255)                                                                                                                            gACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAAtct                                                                           <  1:290851/251‑1 (MQ=255)                                                                                                                                               gAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATTCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTgtcgt                                                         <  2:109222/250‑1 (MQ=255)                                                                                                                                                    aTCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGTTTTATGTTGTCGTCAGGCt                                                   >  2:255846/1‑251 (MQ=255)                                                                                                                                                        gCATTTTGTCACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCCTCATCTGGAGCTGTGACTAAACCCTCAACAATCTCCGTTTCTTCAGTGGCTAGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTAGGCAACCGGCAATTAGTGACGGTTTGTTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTTGTGTATGTTGCCGTCAGGCTCATg                                               <  2:216446/251‑1 (MQ=255)                                                                                                                                                             ttGGTAACCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCgg                                           <  1:586264/250‑1 (MQ=255)                                                                                                                                                                          agGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAg                             <  1:119753/251‑1 (MQ=255)                                                                                                                                                                              aGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGAt                         <  1:255846/251‑1 (MQ=255)                                                                                                                                                                                    tACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTTGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTatat                    <  1:366687/250‑1 (MQ=255)                                                                                                                                                                                       gCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGAAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGAATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGAGATGAGCGTTTATAttt                  >  2:429341/1‑249 (MQ=255)                                                                                                                                                                                            aaTCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGtgtg            <  2:551197/250‑1 (MQ=255)                                                                                                                                                                                                  tttCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGaata      >  1:152757/1‑250 (MQ=255)                                                                                                                                                                                                     cGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAAttt  <  2:51427/251‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       GAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATTT  >  NC_000913/1161187‑1161633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑