Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,146,384	A→G	V71A (GTA→GCA)	yceF ←	m(7)GTP pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,384	0	A	G	100.0%	132.7 / NA	41	V71A (GTA→GCA)	yceF	m(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/24);  total (17/24)

CCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCGTATTGATG  >  NC_000913/1146151‑1146626                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   ccGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCaat                                                                                                                                                                                                                                    >  1:208888/1‑250 (MQ=255)      cAGTGCAGGGGAGGCTCTTTACGCACGTAATTATCAATCGCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCGGGTCTGTACCAATAATTaa                                                                                                                                                                                                                              <  2:550222/251‑1 (MQ=255)       cgttcAGGGGATGCTCTTTACGCACTTAATTATCAATCTCCGCCTCTCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGACGGTGACGATATTGCCGCTTGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGATCACATGCCTGGTCTGAACCAATAATTaaa                                                                                                                                                                                                                             <  1:427912/247‑1 (MQ=255)       aGTGCAGGGGATGATCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTAGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGAGACGATATTGCCGCGGGCTTTGCGTAATTGCAGACGGGCATATTCTTCCGTTAACGGTTTACCGGAGATTTCGCCATCAAGAACACATGCCTGTTCTGAACCAATAATTaa                                                                                                                                                                                                                              >  2:112599/1‑250 (MQ=255)        gTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACATCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAAt                                                                                                                                                                                                                            <  2:566072/251‑1 (MQ=255)                                      aTCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCATTTCAGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGAGCCGGATAACGTGCCGCCATTGATTgcg                                                                                                                                                                                              <  2:80268/251‑1 (MQ=255)                                               cGCCTCGCTCCGGTGACGGATGTGGACGTCCAAAGGCTCCTCCTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCAAGTATTGAAGGTGACGATATTGCCTCTGGATTTGCGTAATTGTAGACGGGCATTTTATTCCGTTAACGGGTTACCGGTGATTTCGCCACCAAGAACACATGCCTGGTATGAACCAAGAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCtt                                                                                                                                                                                     <  1:253225/251‑1 (MQ=255)                                               cGCCTCGCTCAGGTGACGGAAATGGACTTCAAAAGGCGCCACGTCTGTGTGCAGATGCCCATTCGCCGAATCAAACAACGCCAGTCCAGTATAGAATGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAATGGTTTACCGGTGAGTTCGCCATCAATAACACTTGCCTGGTCTGAACCAATAATTAAATGATCTGGATAACGTGACGCCATATATTGAGCTTGTTCtt                                                                                                                                                                                     <  2:270273/251‑1 (MQ=255)                                                gCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTtg                                                                                                                                                                                    <  1:338195/251‑1 (MQ=255)                                                    cGCTGAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTTCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAATGGGACGAGATTGCCGGTGGCGTTGCTTAATTGCAGAGGGGCATTTTCTTCCGTTAACGGTTTACCGGGGATGGCGCCATTAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAGCGGGACGCCAGAGATTGCGCTTTTTCTTGTGcc                                                                                                                                                                                <  1:269535/251‑1 (MQ=255)                                                        cAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGt                                                                                                                                                                             <  1:40119/250‑1 (MQ=255)                                                         aGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCg                                                                                                                                                                           <  2:55527/251‑1 (MQ=255)                                                                       gACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACACCGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTc                                                                                                                                                             >  1:87560/1‑251 (MQ=255)                                                                       aaCGTCAAAAGGCCACACTTCAGTTTTCAGATGCACATTCGCCGAATTAACCAACGCAAGTCCAGTCTAGACGGTGACGATATCGAAGCTGGCTTTGCGTAATTGCAGACGGGCCTTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGCCCAAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTTTGCCAGTCGAAGCACCAACTGTc                                                                                                                                                             >  2:578193/2‑251 (MQ=255)                                                                        aCGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTcg                                                                                                                                                            <  1:233114/251‑1 (MQ=255)                                                                           tCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCgg                                                                                                                                                         >  1:327850/1‑251 (MQ=255)                                                                                                            atCGCCGAATTAAACAACGCCAGTACAGGATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTGTACCGGTGATTTCGCCATCAAGAACACATGCCTGGGCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGAccgc                                                                                                                        <  2:21175/250‑3 (MQ=255)                                                                                                             tCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACctct                                                                                                                       >  1:162357/1‑251 (MQ=255)                                                                                                                                 agtccagtATAGAAGGTGCCGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGCACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCaaa                                                                                                      >  2:433597/1‑248 (MQ=255)                                                                                                                                         ataGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTAGTCGACCTCTGGTGCTGCACATTCAAAAGCGATTTGc                                                                                            >  1:535637/1‑250 (MQ=255)                                                                                                                                           aGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGc                                                                                            >  1:266019/1‑248 (MQ=255)                                                                                                                                           aGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAg                                                                                          <  2:266019/250‑1 (MQ=255)                                                                                                                                            gAAGGTGACGATAGTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGtt                                                                                        <  1:207052/251‑1 (MQ=255)                                                                                                                                                  gACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGTGCATTTTCTTCCGTTAACGGTTTTCCGGTGATTTAGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGGGCCAGTCGAAGCACCAACTGGCTCGGTGATTCGTCGCGGCGTGGGGTGTCGTCGACCTCTGGGGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCa                                                                                  <  1:277701/251‑1 (MQ=255)                                                                                                                                                             cGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGcgcg                                                                        >  2:186249/1‑250 (MQ=255)                                                                                                                                                                tGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACTAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGc                                                                    >  1:121493/1‑251 (MQ=255)                                                                                                                                                                      tGCGTAATTGCAGACGGGCATTTTCTTCCGTTCACGGATTACCGGTGATTTCGCCATCAAGAACACATGCCTGTTATGAAACAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGAACTCTGGTGCTGCACATTCAAAAGCGATTTGCAGTTTTTCCAGAAGCGCGCGACGCCATGGc                                                              >  1:343866/1‑251 (MQ=255)                                                                                                                                                                      tGCGTAATTGCAGACGGGCATGTTCGTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCTCTTTTTCTTGTGCCAGTCGAAGCACCAACTGGCGCTGTGATTCGTCGCTGCGTGGGGTTTCGCCGACCTCTGGTGCTGCACATTCAAAAGAGAGTGGTAGTTGGTCCAGAAGAGCGCGACGCCATgg                                                               <  2:630456/250‑1 (MQ=255)                                                                                                                                                                                 aGACGGGAATTTTCTTCCGTTAACGGTTTACCGGTGAGTGCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGAGTGCGCTTTTTCGTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGTCGAGGTTGAGGc                                                   <  2:327850/251‑1 (MQ=255)                                                                                                                                                                                         aTTTTCTACCGTTAACGGTGTACCGGGGATTTCTCCATCAAGAACACATGCCTGGTCTGAACCAATAAGTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTGTCTTGTGCCAGTCGAAGGACCAAAGGTCGCGGTGATTCGTTTCGGCGTGGGGTTTCGTCGCCCTCTGTTGCTGCACATTCAAAGTAGATGTGGAGTTGTTCCAGTAGGGCGCGTCGCCATGTCGAGGTTGAGGCTTAAATaa                                           <  2:604781/251‑1 (MQ=255)                                                                                                                                                                                           tttCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAg                                          <  1:186249/250‑1 (MQ=255)                                                                                                                                                                                                tcCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGttt                                       <  1:433597/248‑1 (MQ=255)                                                                                                                                                                                                         ggTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATg                                                                >  2:261673/1‑214 (MQ=255)                                                                                                                                                                                                         ggTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATg                                                                <  1:261673/214‑1 (MQ=255)                                                                                                                                                                                                             tACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGCAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTcc                       >  2:252734/1‑251 (MQ=255)                                                                                                                                                                                                                       ttCGCCATCAAGAACACATGCCTGGTCTGATCCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGatat              <  1:321143/250‑1 (MQ=255)                                                                                                                                                                                                                          gCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCg          >  1:381826/1‑251 (MQ=255)                                                                                                                                                                                                                            cATCAAGAACACATGCCTGGTCTGAACTAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCGTa        <  2:121493/251‑1 (MQ=255)                                                                                                                                                                                                                             aTCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGCGATTGAGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCGTAt       >  2:582471/1‑251 (MQ=255)                                                                                                                                                                                                                               cAAGAACACATGCCTGGTCTGAACCAATACTTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCGTATtg     >  1:167891/1‑251 (MQ=255)                                                                                                                                                                                                                                  gAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCGTATtgatg  <  1:252734/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   CCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCGTATTGATG  >  NC_000913/1146151‑1146626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑