Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,144,974	C→T	A12V (GCT→GTT)	rluC →	23S rRNA pseudouridine(955/2504/2580) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,144,974	0	C	T	100.0%	122.3 / NA	40	A12V (GCT→GTT)	rluC	23S rRNA pseudouridine(955/2504/2580) synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/19);  total (21/19)

TTCCACTGCAAGCCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGCTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTG  >  NC_000913/1144739‑1145194                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             ttCCACTGCAAGCCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACg                                                                                                                                                                                                                >  2:53124/1‑250 (MQ=255)   ccACTGCAAGCCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGaa                                                                                                                                                                                                              <  1:573855/250‑1 (MQ=255)         cAAGCCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGc                                                                                                                                                                                                       >  1:338176/1‑251 (MQ=255)            gCCTTGTTACAGCAGAACGACTTTTACCATCTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCCCGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGAATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAACAATTGTTGTTATCACCGCTGACGAAGCGGGGCacc                                                                                                                                                                                                    >  2:94707/1‑249 (MQ=255)             ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGAc                                                                                                                                                                                                                 >  1:286415/1‑237 (MQ=255)             ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGAc                                                                                                                                                                                                                 <  2:286415/237‑1 (MQ=255)                      aGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATa                                                                                                                                                                                           <  1:56706/250‑1 (MQ=255)                           gATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAAGATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCAGCCGTAAAAATTGGTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGAGAACtttt                                                                                                                                                                                     <  2:369625/251‑1 (MQ=255)                                 ttttACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGt                                                                                                                                                                                >  1:49286/1‑250 (MQ=255)                                                 ccGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCTTAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGt                                                                                                                                                               >  1:446620/1‑251 (MQ=255)                                                                           ttGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTACGTATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGAAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACTAAAAAGTATGATTTACCGTAttt                                                                                                                                     >  1:627694/1‑251 (MQ=255)                                                                                ggTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGt                                                                                                                                <  1:355598/251‑1 (MQ=255)                                                                                         tttAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGa                                                                                                                        >  1:90368/1‑250 (MQ=255)                                                                                                  cATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTg                                                                                                              <  1:48162/251‑1 (MQ=255)                                                                                                    tCAATAAAGAGATCTCGCAGAGCAGTAATCAGGTGCAAAATTAAATCTAAGCATAGAAAAATTAGTGGCACGAATCCTTTTGGCTAATTAGCATCACCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGCAGCGGGGCAACGTATCGATACCTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATCATTTACCTTATTTTTCTTAAAGGCGAAGTGCgtggga                                                                                                             >  2:287/1‑245 (MQ=255)                                                                                                      aaTAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAAc                                                                                                           >  2:569024/1‑250 (MQ=255)                                                                                                                       gTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCt                                                                                         <  1:447345/251‑1 (MQ=255)                                                                                                                          aaGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGaa                                                                                      <  2:342735/251‑1 (MQ=255)                                                                                                                              aataaGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAatat                                                                                   <  2:453607/250‑1 (MQ=255)                                                                                                                              aataaGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAatat                                                                                   <  2:321526/250‑1 (MQ=255)                                                                                                                              aataaGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGGATTAAGCCTGAatat                                                                                   <  1:473119/250‑1 (MQ=255)                                                                                                                                  aGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACt                                                                              >  2:140003/1‑251 (MQ=255)                                                                                                                                    gTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCg                                                                            >  1:217951/1‑251 (MQ=255)                                                                                                                                         aaaatAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAAACGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTGATCACCGCTGACGAAGCGTGTCAACGTATCGATAACGTTTTGCGGACCCAATGGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCTAAGTGCGGGTGAACGAAAAACGAATTAAGCCTGAATATAATCTCCAAGCg                                                                       <  2:85425/247‑1 (MQ=255)                                                                                                                                          aaGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCgg                                                                      >  1:470840/1‑251 (MQ=255)                                                                                                                                            gTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGt                                                                    <  2:523474/251‑1 (MQ=255)                                                                                                                                               aaTATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGACGTGCGGGTGAACCAAAAACGTATTATGCCTGAATATAAACTCGAAGCGGGtgat                                                                 >  1:310571/1‑251 (MQ=255)                                                                                                                                                 tataAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGtgatg                                                                >  1:370885/1‑250 (MQ=255)                                                                                                                                                        aTAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTAttt                                                                                                                                     <  1:450991/174‑1 (MQ=255)                                                                                                                                                        aTAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTAttt                                                                                                                                     >  2:450991/1‑174 (MQ=255)                                                                                                                                                          aGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTa                                                       <  2:217951/250‑1 (MQ=255)                                                                                                                                                            aaaaaTGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGAATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGGGCGTATTc                                                    >  2:26542/1‑251 (MQ=255)                                                                                                                                                                 tGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTACAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACCAAAACCGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCaacg                                               >  1:630500/1‑248 (MQ=255)                                                                                                                                                                               ccTGTTGGCTAATTAGAATCGCCAACCAGGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGa                                 <  1:521888/251‑1 (MQ=255)                                                                                                                                                                                cTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTTCGTATTCCACCGGTTCGCGTTGCTg                                  >  1:603953/1‑249 (MQ=255)                                                                                                                                                                                                   gctaaCCATGAAAACAGATACTACAAACATAAAAATTGGTGTCAACCCCGCTGACAAAGCGGGGAAACGTATCGATAACTTTGTGCGTACCCTATTGAAAGGCGTACCAAAAAGTATGATTTGCCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGACTGAATATAAACTCGAAGCGGGTGATAAGGTGCGTATTCCACCGGTTCTCGTTGGTGATCGGGAAGAAGAGGCGGtgt             <  2:3228/248‑3 (MQ=255)                                                                                                                                                                                                   gCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGttt             >  2:168550/1‑251 (MQ=255)                                                                                                                                                                                                     cAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAATCTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCg           <  1:362687/251‑1 (MQ=255)                                                                                                                                                                                                         cATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATACCTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTTGcccc       >  1:521405/1‑249 (MQ=255)                                                                                                                                                                                                              aaaCAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTg  <  2:521405/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             TTCCACTGCAAGCCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGCTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTG  >  NC_000913/1144739‑1145194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑