Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,138,152	C→T	T261I (ACC→ATC)	flgJ →	putative peptidoglycan hydrolase FlgJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,138,152	0	C	T	100.0%	166.9 / NA	56	T261I (ACC→ATC)	flgJ	putative peptidoglycan hydrolase FlgJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/33);  total (23/33)

ACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAAC  >  NC_000913/1137907‑1138398                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       aCTGGCATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAAACCGCGCTACGCCGCCGTGACGAtcgccg                                                                                                                                                                                                                                                   >  2:372303/1‑251 (MQ=255) aCTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGGTTACGTTGGGATGTTAACGCGTAACCCGCGCTACGCCGCCGCGACGAtcgccg                                                                                                                                                                                                                                                   >  2:132428/1‑251 (MQ=255)                tgggCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACCGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAATGCGGAACAgg                                                                                                                                                                                                                                    >  1:579898/2‑251 (MQ=255)                ggggCAACGGCAAATCCACAGCGAAAACGGAGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAgg                                                                                                                                                                                                                                    >  1:174433/1‑251 (MQ=255)                          aaaaTCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGc                                                                                                                                                                                                                          <  2:406990/250‑1 (MQ=255)                              tacgccgcGAAAACAGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGACTATGGCAAATGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGACGCGAAGAAAGTAAAAGCGAAGATTCGCGTCTACAGCTCGGATCTGGAAGACTTGTCGGATTACGTTGGGATGTTAACGCGTAACCCGCGCTACGCCGCCGCGTCGATCGCAGCGAGAGCGGTACGGGAGGCGCACGCCCt                                                                                                                                                                                                                       >  2:534906/3‑250 (MQ=255)                                   cgcgAAACCGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACGGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGGCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCTTACAGGa                                                                                                                                                                                                                 <  2:269586/251‑1 (MQ=255)                                         aaCGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCggt                                                                                                                                                                                                           <  2:346422/251‑2 (MQ=255)                                              cgagccgagcTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATg                                                                                                                                                                                                      <  2:131702/251‑1 (MQ=255)                                                agccgagcTATAACCTTTTGGGTGTCAAATCCTCTGGCAACTGGAAAGGGCCAGTTACTTAAATCACCGCGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGGTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGcc                                                                                                                                                                                                    <  2:17628/251‑1 (MQ=255)                                                  ccgagcTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCAc                                                                                                                                                                                                  >  1:435654/1‑251 (MQ=255)                                                   cgagcTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCAcc                                                                                                                                                                                                 >  2:489122/1‑251 (MQ=255)                                                         ataACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCt                                                                                                                                                                                           <  1:366990/251‑1 (MQ=255)                                                                gTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGCCTGAATATGAACACGGCGAAGCGTAGAAAGAACAAGCGACGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTAAGCCGCCGTGACGATAGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGAGCGAGGGCTATGCCACCGATCCTCACTATg                                                                                                                                                                                    >  2:247252/1‑251 (MQ=255)                                                                         aaaaGCCTCTTGCAACTGGAAATGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGGTTACATCTCGTATCTGGAAGCCTTTTCGGATTACGTTGGGCTGTTAACGCGTAACCCCTGCTTCGTCGCCGTGACGATCGCCGCTAGTTCGGAACATGGGGCTCAGGCTCTACATGACGCGGGCTATGCCACCTGTCCTCTCTATGCCCGCAAAc                                                                                                                                                                           <  2:589030/250‑1 (MQ=255)                                                                                 ctGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCa                                                                                                                                                                     <  1:166228/249‑1 (MQ=255)                                                                                   ggCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAACCTCACCAACAt                                                                                                                                                                 >  2:279603/1‑251 (MQ=255)                                                                                    gCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATg                                                                                                                                                                <  1:209987/251‑1 (MQ=255)                                                                                       aCTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGAGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTATGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGTCCCTACCGGCCGCGGGCTATGCCACCGATCCTCACTCTGCCCGAAAACTCACCAACATGAtt                                                                                                                                                             >  2:138899/1‑251 (MQ=255)                                                                                         tGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGGTCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTc                                                                                                                                                            >  2:156919/1‑250 (MQ=255)                                                                                          ggAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcag                                                                                                                                                          <  1:190591/251‑1 (MQ=255)                                                                                          ggAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTc                                                                                                                                                            <  1:319210/249‑1 (MQ=255)                                                                                           gAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcagc                                                                                                                                                         >  2:481549/1‑251 (MQ=255)                                                                                              aGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCCCTATGCCCGCAAACTCACCAACATGATTcagcagt                                                                                                                                                      <  2:472423/251‑2 (MQ=255)                                                                                                            aaaTCACCGCGAATGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGc                                                                                                                                        <  2:214288/251‑1 (MQ=255)                                                                                                             aaTCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCg                                                                                                                                       <  1:489122/251‑1 (MQ=255)                                                                                                              aTCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCCCTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCg                                                                                                                                       >  2:420177/1‑250 (MQ=255)                                                                                                                            atatGAAAACGGCGAAGATAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGGATCTGGAAGCCTGGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATTCTCACTATGCCCGAAAACTCACCAACATGAGGCAGCAGATGAAATCGATAAGCGACAAGGTGAGCaaaa                                                                                                                        <  2:579898/251‑1 (MQ=255)                                                                                                                                 aaaaCGGCGATGCGAAAAAAGTAATAGCGAATTTTCGCGTCTACAGCTCGTATCTGGAAGCCGTGTCGGATTACGTTGGGCTGTTAACTCGTAACCCGGTCTACGCCGCCGTGACGATCGTCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACATGACGCGGGCTATGCCTCCGATCCTCACTAGGCCCGCTAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTAc                                                                                                                   <  1:77987/251‑1 (MQ=255)                                                                                                                                  aaaCGGCGAAGCGAAGCAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTAc                                                                                                                   >  2:599750/1‑250 (MQ=255)                                                                                                                                   aaCGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGAACCTCACTATGCCCGCAAACTCACCACCATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTAcc                                                                                                                  >  1:492697/1‑249 (MQ=255)                                                                                                                                     cGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGGGAGCAAAACCTACAGt                                                                                                                >  2:330230/1‑250 (MQ=255)                                                                                                                                      ggCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTAt                                                                                                              >  1:227218/1‑251 (MQ=255)                                                                                                                                      ggCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCGCGTATCTGGAAGCCTTGTCGGATTTCGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACGCACCAACATGATTCAGCAGATGAAAGCGATAAGCGACAAGGTGAGCAAATCCTACAGTAt                                                                                                              <  2:238112/251‑1 (MQ=255)                                                                                                                                          aagcgaagAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAg                                                                                                                 >  1:453094/1‑244 (MQ=255)                                                                                                                                          aagcgaagAAAGTAAAAGCGAAGGTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAg                                                                                                                 <  2:453094/244‑1 (MQ=255)                                                                                                                                          aagcgaagAAAGTAAAAGAGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGACGGATTACGTTGGGCTGTTAACGCGTAACCCGCGATACGCCGCCGTGACGATCGCCGCGAGTGAGGAACCGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGAAAAGCGACAAGGGGAGCAAAACCTACAGTATGAAc                                                                                                          >  2:592203/1‑251 (MQ=255)                                                                                                                                               aagaaAGTAAAAGCGAAGGTTCGCGTCGACAGCTCTTATCTGGAAGCCTTGTCGGAGTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCGCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGAGTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGa                                                                                                     <  1:247252/251‑1 (MQ=255)                                                                                                                                                agaaAGTAAAATCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGAt                                                                                                    <  2:382819/251‑1 (MQ=255)                                                                                                                                                  aaaGTAAAAGCGAAGTTTCGCGTCTACCGCTCGTATCTGCAAGCCTTGTCGGATGACGTGGGGCGGTTAACGCGTAACCCGCGCTACGCCGCCGTGACTATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGAGGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACACTGATaa                                                                                                  <  2:556775/251‑1 (MQ=255)                                                                                                                                                     gTAAAAGCGAAGTTGCGCGTCTACGGCTCGGATCTGGATGCCTTGGCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCATCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCt                                                                                               <  2:383186/251‑1 (MQ=255)                                                                                                                                                       aaaaGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGt                                                                                             >  2:109208/1‑251 (MQ=255)                                                                                                                                                            cGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGa                                                                                        <  2:611061/251‑1 (MQ=255)                                                                                                                                                                  ttCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACt                                                                                  >  1:10397/1‑251 (MQ=255)                                                                                                                                                                   tCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCACCATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTATGAATAACTc                                                                                 >  2:204270/1‑251 (MQ=255)                                                                                                                                                                            aaGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAAACGATAAGCGACAAGGGGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCcggc                                                                        >  2:346428/2‑251 (MQ=255)                                                                                                                                                                             aGCTCGTCTCTGGAAGCCTTGTCGGATGACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCcggcg                                                                       <  2:74954/251‑1 (MQ=255)                                                                                                                                                                              gCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGCACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCcggcgg                                                                      >  1:413731/1‑251 (MQ=255)                                                                                                                                                                              gCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCcggcgg                                                                      <  1:39136/251‑1 (MQ=255)                                                                                                                                                                                                tGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGataata                                                      <  2:213778/249‑1 (MQ=255)                                                                                                                                                                                                              gggCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTATAATTGAAGGc                                      <  1:599750/251‑1 (MQ=255)                                                                                                                                                                                                              gggCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGGCGCTGCCGATAATACTCTGTAATTGAAGGc                                      >  2:261577/1‑251 (MQ=255)                                                                                                                                                                                                                       aCGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGa                             <  1:379209/251‑1 (MQ=255)                                                                                                                                                                                                                          cggaaCCCGCGCTACGCCGCCTTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACGCACCAACAGGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGGCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAAcc                          <  1:616545/248‑1 (MQ=255)                                                                                                                                                                                                                                  gcgcTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTc                  <  1:6203/251‑1 (MQ=255)                                                                                                                                                                                                                                   cgcTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTcc                 <  1:279603/251‑1 (MQ=255)                                                                                                                                                                                                                                       aCGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGc              <  2:227218/250‑1 (MQ=255)                                                                                                                                                                                                                                          ccgccgTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGa          <  1:420177/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gacgatCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATtaata    >  1:117197/1‑250 (MQ=255)                                                                                                                                                                                                                                                  acgatCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAAc  <  1:372303/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       ACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAAC  >  NC_000913/1137907‑1138398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑