| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,130,210 | G→A | N218N (AAC→AAT) | flgA ← | flagellar basal body P‑ring formation protein FlgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,130,210 | 0 | G | A | 96.5% | 75.4 / NA | 29 | N218N (AAC→AAT) | flgA | flagellar basal body P‑ring formation protein FlgA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (17/11); minor base T (0/1); total (17/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTAC‑CCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCA > NC_000913/1129967‑1130453 | gcCGGGTTGCATCAGTTTTGCTTGCGCGTCTCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATaa < 1:498153/251‑1 (MQ=255) gggTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCCCACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGaa < 2:451348/251‑1 (MQ=255) gCATCAGTTTTGCGTGCGCGTCGCTTAACGTCACACTGGTGCTGGGGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCCTACAGATTTATAAGAATATTc < 1:265948/251‑1 (MQ=255) cATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTTCCGCCCCGCTGTTTGGTACCGGCGCGTCAGAGGTTTCGCGCGGTTGAAAGGGGCTTAACGGCTTTAGAGGCGAAGTGCGATCAATACTCATGGGTTATTTCTCATTGAGGGCGCCTTTTTCCTTGGTTGCTTATTTTTCGGTAA‑GGGACGGGGAATCTTTAACAGCTTTCAGATTTATAAGAATTTTcc > 1:242856/1‑251 (MQ=255) aaCGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTGAGGGGTTTCTCGCGGGTGAACGGGGCTTATAGGCTTCCGAGGCGACGTGGGGTCAATACTCCTGGTTTATTTCTCATTGAGGGCGCTTTTTTTATGTGTTGCTTTTTTTTTGGCAAAGGGA‑GGGGAATCTTTTAAAACTTACAGATTTTTTAAAATATTTCCCTCTGCaacaac > 1:162791/1‑239 (MQ=255) aaCGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATcaac < 2:162791/239‑1 (MQ=255) ggTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGCGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGATTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCAccc > 2:523148/1‑251 (MQ=255) gttgttTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCcg > 2:566145/1‑251 (MQ=255) gccgccCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCtg > 2:453172/1‑251 (MQ=255) gcgTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAgcgc < 1:325196/251‑1 (MQ=255) tCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTg > 1:310756/1‑251 (MQ=255) tGGTTTCGCGTGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCt > 2:242006/1‑251 (MQ=255) ggTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCtt < 2:357356/251‑1 (MQ=255) ttCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCt < 1:126490/250‑1 (MQ=255) gagGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATc > 2:57084/1‑251 (MQ=255) ggCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATcac > 1:37129/1‑251 (MQ=255) cGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCCCCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGAcgcg < 1:102584/251‑1 (MQ=255) gATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGt > 1:324479/1‑251 (MQ=255) aTCACTACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGCAATCTTTAACAGCTTTCAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGCCGCCTGCCTTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCCTCACCGCTGGCGATCACATTGACGCGtt > 2:331532/1‑251 (MQ=255) gggCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTAC‑CCGCCATGCCTGGCg > 1:473893/1‑250 (MQ=255) ttATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTAC‑CCGCCATGCCTGGCGAAACTGGGt > 1:127458/1‑251 (MQ=255) tttagcggaaggggAGGGGTAATTTTTAACAGCTTACAGATTTATAAGAATTTTCCCATCTGCATCAACAACGCCGCTGATTCCTTGTCCCGATACCATGCGCCCCCGCGCATTCTGTGCGACGGCTGCATTGTCCAGCGCCTGCCTTTCTGGGTTGGCGCTAAACCCATCCCCGCGGGCGATCACATTGACGCTTTTTCCCGCTTTTAC‑CCGCCATGCCTGGCGAAACTTGGTTAACTGGATAGGTTGa < 1:523148/239‑1 (MQ=255) ggCAA‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTAC‑CCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTg > 1:66858/1‑251 (MQ=255) aa‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTAC‑CCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGAt > 1:45029/1‑250 (MQ=255) a‑GGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCtgtg > 2:523677/1‑109 (MQ=255) a‑GGGACGGGTAATCTTTAACAGCCTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCTCTGACTACCTGACCCGATACCATGCGCACCCGCGCATTCtgtg < 1:523677/109‑1 (MQ=255) tAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTAC‑CCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGTCTactg > 2:212415/1‑248 (MQ=255) aaCAGCTTACAGTATTATAAAAATATTCCCATCTGCACAAACACCGCCGCTGACCCCCTGTCCCGACACCATGCGCACCCCCGCATTTTGTGCGCCGGCTGCATTGTTCAGCGCCTGCCCTTCGGCGTTGCCGCTAACCCCATCCCCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTAC‑CCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATgg < 2:431635/251‑1 (MQ=255) cgtACAGATTTATAAGAATATCCCCAGCTGCATCAACAACGCCGCTGACTACATGTCCCGATCCCAGGCGCCCCCGCGCATGCTGTGCGCCGGCTGCATGGTTCAGCGCCTGACCTTCGGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGTGC‑GGCCCCCTTTTTCACCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATca < 2:5815/249‑1 (MQ=255) | GCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAA‑GGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTAC‑CCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCA > NC_000913/1129967‑1130453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |