Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,129,796	G→A	S12F (TCC→TTC)	flgN ←	flagellar biosynthesis protein FlgN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,129,796	0	G	A	88.1%	101.5 / ‑5.2	42	S12F (TCC→TTC)	flgN	flagellar biosynthesis protein FlgN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (19/18);  minor base C (5/0);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 6.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTATGTTGATTCATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGG‑TTGTTTTTGCC  >  NC_000913/1129551‑1130039                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      cGTTATGTTGTTGCATTGGGCGTTGTTGCTGCGTTTTTACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCAGTATTGGGTGCTGTCCTTCGTAACTGTTCGAGTTAATCCAGCGGCGCCAGCCGTGAGCTTGTTTGGGCTGTAAGTCATGGCAACGTGCTGCCGTTGGACTGCCCCATATAGAAATGGTACTGCTCGTGGGCCATGACCGTTGCGAGATCGATAAGCTCAGCGAACATc                                                                                                                                                                                                                                                  <  2:98015/251‑1 (MQ=255)              cATTTGGCGAAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTACTATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATCGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGCACATCTGGTCGAGGAtct                                                                                                                                                                                                                                     >  2:357816/1‑251 (MQ=255)                  tGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGc                                                                                                                                                                                                                                 >  2:23634/1‑251 (MQ=255)                    gCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTACTATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCa                                                                                                                                                                                                                                >  2:259585/1‑250 (MQ=255)                                            aaTTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTAct                                                                                                                                                                                                       <  1:359163/250‑1 (MQ=255)                                             aTTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGTTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGGTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCTTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATTCGATCAGTTActct                                                                                                                                                                                                     <  2:540316/251‑1 (MQ=255)                                                tCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGc                                                                                                                                                                                                   <  2:628758/250‑1 (MQ=255)                                                 ccTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCa                                                                                                                                                                                                  >  1:230900/1‑250 (MQ=255)                                                   tGCCAGCGTTGACTAATCTCAACGCTATCTGCTGCATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCACTTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAGGACGTG‑TCATACGATCAGTTACTCTGCAAGt                                                                                                                                                                                               >  2:498153/1‑251 (MQ=255)                                                             gACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTgcgc                                                                                                                                                                                     >  1:229069/1‑251 (MQ=255)                                                                    atCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTg                                                                                                                                                                              <  2:347360/251‑1 (MQ=255)                                                                             aTTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAgcgc                                                                                                                                                                      <  1:545633/250‑1 (MQ=255)                                                                                    gTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCa                                                                                                                                                               >  1:70924/1‑250 (MQ=255)                                                                                         gggTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCGTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGATGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTg                                                                                                                                                         <  1:288065/251‑1 (MQ=255)                                                                                                 tCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGgtgt                                                                                                                                                  <  2:482464/250‑1 (MQ=255)                                                                                                           cTGTTGGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTACTCCATTGCCACTGGCTGACGTTGATCTGCCCCATAGTGAGCTGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGCACATCTgg                                                                                                                                                                                                                                               >  2:566618/1‑148 (MQ=255)                                                                                                           cTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTgg                                                                                                                                                                                                                                               <  1:566618/148‑1 (MQ=255)                                                                                                             gTTCGAGGTAATCCAGCGGCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCGTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGtt                                                                                                                                      <  2:48212/250‑1 (MQ=255)                                                                                                                        tCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGa                                                                                                                           >  1:357356/1‑250 (MQ=255)                                                                                                                             agTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGCACATCTGGTCGAGGCTCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGCCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGcc                                                                                                                     >  2:3310/2‑251 (MQ=255)                                                                                                                                   cagctgTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGGTGCTGCTCTTGATCCATTACCGTTTTGAGCTCGGTAAGCACAGCGAACATCGTGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTGACTCTGCAATTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCGATTTTGCCGGTGTCCATTTTCAGTGCACCGTTACGAATCGCCTGtttt                                                                                                               <  2:576780/251‑1 (MQ=255)                                                                                                                                    agcagTGCGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAACGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGACTCGCCAGtttt                                                                                                               >  1:559464/1‑250 (MQ=255)                                                                                                                                         tGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGtt                                                                                                                                                                               >  1:95095/1‑181 (MQ=255)                                                                                                                                         tGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGtt                                                                                                                                                                               <  2:95095/181‑1 (MQ=255)                                                                                                                                                ttttGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGacac                                                                                                  >  1:451348/1‑251 (MQ=255)                                                                                                                                                         gTAATCCATTGCAACTGGCTTCCGTTTATCTGCCCCATAGAAAGATGGTGCTGCTCTTGATCCATTTCCGTTTTTAGAACCTTTAGCACAGCGAACATCTGGGCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTTCTGCGCTTCGTTGATTCGCGCATCGGCAATTTTTCCGGGGTCCATTTTTATTTCACCGTTACGAAGCGCCAGTTTTAACGCTTCGACACGTTCAAg                                                                                           <  2:301469/249‑1 (MQ=255)                                                                                                                                                             tCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCCCAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTCCGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGat                                                                                     >  2:223769/1‑251 (MQ=255)                                                                                                                                                               cATTGCAAATGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGATGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCCGCAAGTCTTGCTGCGCTTAGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCAAGTTTTAACGCTTCGACACGTTCAAGATTGatat                                                                                   >  1:396530/1‑251 (MQ=255)                                                                                                                                                                aTTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGCTCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCAAGGATCTCTGCAAAAC‑TGATCATACGATCCGTTACTCTGCAAGTCTTGCAGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAAACGCCAGTTTTAACGCTTCGAAACGTTCAAGATTgcaatc                                                                                  >  1:605686/1‑246 (MQ=255)                                                                                                                                                                   gCAACTGGCTGCCGTAGATCTGCCCAATAGAGAGCTGTTGCTGATCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACCTCTGGTCGCGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGAATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGCATCGCCAGTTTTAAAGCTTCGACACGTTCCAGCTTGATATCAct                                                                               >  2:102584/1‑251 (MQ=255)                                                                                                                                                                               cGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTg                                                                   <  2:230900/251‑1 (MQ=255)                                                                                                                                                                                    aTCTGCCCACTAGAGAGATGTTGCTGCTCTTGCTCCATTACCGTTTTGAGATCGTTAAGCACCGCGCACATCTGGTCGCGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATc                                                               >  2:280955/1‑250 (MQ=255)                                                                                                                                                                                       tGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTAACGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGCTCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTCGTTCACCGTTACGACTCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCCCTGCTGCCGGGCTGCATCAGtt                                                           >  2:126490/1‑251 (MQ=255)                                                                                                                                                                                              tAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGCACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATCCGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGCTTGATATCACTGCTGCCggg                                                                      >  2:44792/1‑233 (MQ=255)                                                                                                                                                                                              tAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCggg                                                                      <  1:44792/233‑1 (MQ=255)                                                                                                                                                                                                         tgctgcTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCtt                                          <  2:229069/250‑1 (MQ=255)                                                                                                                                                                                                                            ccGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGAG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCAGGTGTCCATTTTTAGTTCACCGTTACGACTCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACActggcgctggt                      >  2:444591/1‑251 (MQ=255)                                                                                                                                                                                                                             cGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACActggtgctggtg                     <  1:357816/251‑1 (MQ=255)                                                                                                                                                                                                                                           taagcgaaGCGAACATCTGGTCGAGGATCTCTGCAAGACGAG‑TCAAACGATCAGTTACTCTGCATGTCTTCCTGCGCTACGTTGCTCAGCACATCGGCAATTTTGCCGATGTCCATTTTTAGTTCAACGTTACGAATCGCCAGTTTTAACGCCTCGACACGTTCAAGATTGCTATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGATTGttt       >  2:289365/8‑251 (MQ=255)                                                                                                                                                                                                                                           tAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGG‑TTGtttt      >  1:75775/1‑251 (MQ=255)                                                                                                                                                                                                                                           tAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGG‑TTGttt       <  1:361700/250‑1 (MQ=255)                                                                                                                                                                                                                                               cacaGCGAACATCTTGGCGAGGCTCTCTGCCAGACGTG‑TCATACGCTCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGAATCGGCAATATTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGCCACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGG‑TTGtttttttc  >  2:331014/1‑248 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CGTTATGTTGATTCATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTG‑TCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGG‑TTGTTTTTGCC  >  NC_000913/1129551‑1130039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑