Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,126,518	C→T	A121V (GCC→GTC)	yceH →	DUF480 domain‑containing protein YceH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,126,518	0	C	T	100.0%	89.4 / NA	29	A121V (GCC→GTC)	yceH	DUF480 domain‑containing protein YceH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/12);  total (17/12)

ATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCGAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGC  >  NC_000913/1126272‑1126755                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              aTCAGAAAACGAACCGTGAACCGGTCATGAATCTGCGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGccgcgtcgcg                                                                                                                                                                                                                                           >  2:458422/1‑251 (MQ=255)  tCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCcgcgtcgcgc                                                                                                                                                                                                                                          >  1:37646/1‑251 (MQ=255)   cAGAAAACGAACCGTGAACCGGGCATGAATCTGAGTGAATCCAAAGTGCAGGAACAGCTTGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCTGAAGTGTCGTTAAGCACCACGTTGTTATTGCGTGGTGCCCAGACGGCAGGGGAACTGCGCAGCcgcgtcgcgc                                                                                                                                                                                                                                          <  2:256051/250‑1 (MQ=255)    aGAAAACGCACCGTGAACCGGTCATGAATCTGCGTGAATCCAAAGAGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCCGGTGAACTGCGCAGCCGCGTcgcgcg                                                                                                                                                                                                                                         >  2:140134/1‑250 (MQ=255)                gTGAACCGGTCATGAATCTGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTATTTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCAAGACGCCCGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGatttt                                                                                                                                                                                                                            >  1:404632/1‑247 (MQ=255)                              aaTCTGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTAATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAATTCTGAGCAACGTTTTTGTACTTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTCTTGCGTGGTGCCCAGACGCCAGATGAACTGCGCAGACGCGTAGCGCGAATGTATGAATTCAGCGatttgacgg                                                                                                                                                                                                               >  1:627979/1‑243 (MQ=255)                              aaTCTGAGTGAATCCAAAGTGCAGGAACACCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGCGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGGGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATCTGGCggg                                                                                                                                                                                                              >  2:262073/1‑250 (MQ=255)                                     gTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAg                                                                                                                                                                                                       <  1:418479/251‑1 (MQ=255)                                           ccaaaGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACGGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGGGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATTAATTCAGCGATATGGCGGAAGTGGAGTCGAc                                                                                                                                                                                                  <  2:33850/247‑1 (MQ=255)                                                  gCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGCGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGGGGAAGCGGCGCTGGaa                                                                                                                                                                                           >  2:582834/1‑250 (MQ=255)                                                              ggATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTAGACGCTGGAACCACTGGCAAATc                                                                                                                                                                              >  2:369984/1‑251 (MQ=255)                                                                                   ttATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTgtgg                                                                                                                                                         >  1:549250/1‑251 (MQ=255)                                                                                       cTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCg                                                                                                                                                     >  1:67007/1‑251 (MQ=255)                                                                                                                      tCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAAc                                                                                                                      >  1:582767/1‑251 (MQ=255)                                                                                                                       cACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAAcc                                                                                                                     <  1:16862/251‑1 (MQ=255)                                                                                                                          cAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGGCGACGCTGGAAAAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCt                                                                                                                  >  1:86606/1‑251 (MQ=255)                                                                                                                           aaaTATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGc                                                                                                                                                                                                                 <  2:346438/155‑1 (MQ=255)                                                                                                                           aaaTATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGGAAACGCGAAAACCGCt                                                                                                                  >  1:46573/1‑250 (MQ=255)                                                                                                                               atGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGGCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAAACCCTACATg                                                                                                             >  2:469956/1‑251 (MQ=255)                                                                                                                                  aGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCAt                                                                                                          <  1:854/251‑1 (MQ=255)                                                                                                                                     aaCGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCtt                                                                                                       <  2:37646/251‑1 (MQ=255)                                                                                                                                               tAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAgtggtg                                                                                             >  1:339534/1‑251 (MQ=255)                                                                                                                                                    cAGAATTGGGCGATCGGAAACTGAGCGCAGCGGAAGTGGCGCTAATCACCTCGTTGTTACCGCGTGGTGCCCAGACGCCAGGTGAACGGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGTAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTGTTGTTGTGCTTCTGGCCCGCGAACCGGGTAAACGCTGAAACCGCTACATTATTCTTTTCAgtgtataggtt                                                                                        <  2:411736/251‑9 (MQ=255)                                                                                                                                                       aaTTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAAGTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGaa                                                                                     <  1:315737/251‑1 (MQ=255)                                                                                                                                                            ggCGATCTGAAACGGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAAAAAATGGCAAATCGCGAAGGTGGGCATTTTGTGGTGTGTCTGGCCCGCGGACCGGGTAAACCCGAAAAACGCATCCTGCATCCTTTCAGTGGGGAGGTTGa                                                                                      >  2:124609/1‑245 (MQ=255)                                                                                                                                                                    gAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGGGCGTCTTGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATTCATCTTTTCAGTGGTTAGGTTGTAGAGCAGCcggcgg                                                                        <  2:404632/251‑1 (MQ=255)                                                                                                                                                                            gcgcAGCGGAAGTGGCGTTAATCCCCACGTTGTTATGGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAAGTCATCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAAGCGCGAAGATGGTCCTTTTGTGGTGCGTCAGGCCCGCGGACCGGGTAAACGCGAAAGCCGTGACATGCAGCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGat                                                                <  2:100904/251‑1 (MQ=255)                                                                                                                                                                                 gCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTc                                                           <  2:549250/251‑1 (MQ=255)                                                                                                                                                                                   ggAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGa                                                         <  2:582767/251‑1 (MQ=255)                                                                                                                                                                                               aaTCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGCGACGGATATGTAGAACGCGGTTGACg                                             >  2:298231/1‑251 (MQ=255)                                                                                                                                                                                                                               cGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGGGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGGCGAAGCCCTggg              >  1:511498/1‑249 (MQ=255)                                                                                                                                                                                                                                          cTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGCGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTAAAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGc  >  1:206574/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              ATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCGAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGC  >  NC_000913/1126272‑1126755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑