Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,762	G→A	V282V (GTC→GTT)	pyrC ←	dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,762	0	G	A	100.0%	139.5 / NA	45	V282V (GTC→GTT)	pyrC	dihydroorotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/14);  total (31/14)

GGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTG  >  NC_000913/1121547‑1122004                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   agAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACAAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTGGCTCTTCACGTACCAGTTCGATGAATGTGTCGGTGACCGGCAACACATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGtt                                                                                                                                                                                                                 <  1:325683/250‑1 (MQ=255)           aTTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGGCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGccc                                                                                                                                                                                                       <  2:116059/251‑1 (MQ=255)            tttttATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCcg                                                                                                                                                                                                      >  1:623753/1‑251 (MQ=255)                     tttAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGc                                                                                                                                                                                             >  1:438181/1‑251 (MQ=255)                           ggACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAACGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGc                                                                                                                                                                                       >  2:398349/1‑251 (MQ=255)                                   gTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTg                                                                                                                                                                               >  2:194490/1‑251 (MQ=255)                                       cGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGAt                                                                                                                                                                           >  2:15451/1‑251 (MQ=255)                                       cGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTGCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGGTGGCCTGCAACCCATAGAACTTCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAATGCATTCATTTCGTCAAAATCGTTAGCGTAACTGCCCAGCGCGGGGTGGGCGTTGAATCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGAt                                                                                                                                                                           <  1:268143/251‑1 (MQ=255)                                        gTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCAAGTACCAGTTCGATGAATGTGTCGTTGCCCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAACAACGGTAGCGTAACTGCCCAGCGCGGTTGCGGCGCTGAAGCAGCCCACGCCGCCGCAACTGCTCTCTTTGCGAtt                                                                                                                                                                          >  1:389883/1‑250 (MQ=255)                                             cccGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTg                                                                                                                                                                     >  1:149657/1‑251 (MQ=255)                                                    aGGACTGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGCGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTACCTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACAGATCTCTTTGCGATGACGTGCATGTgg                                                                                                                                                              >  2:458606/1‑251 (MQ=255)                                                            cACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAAt                                                                                                                                                      >  1:283245/1‑251 (MQ=255)                                                                     atcatcaGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCGAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCgg                                                                                                                                             >  2:498667/2‑250 (MQ=255)                                                                          cagtcagtGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAAt                                                                                                                                        >  1:97488/1‑251 (MQ=255)                                                                            gtcagtGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCAAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATAc                                                                                                                                      >  1:97122/1‑251 (MQ=255)                                                                            atcagtGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGCCGTGCATGTGGCGCAGATTCCGTACCGAGGacaac                                                                                                                                      >  2:498977/2‑247 (MQ=255)                                                                                gtGCGCTGCTTTCAGCAACCTGTTGCTCTTCCCGTACCAGTTCGCTGAATGTGTCGTTGCCCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTACTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGCAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGAGGCGCAGAATCCGTACCGAGGCATACTCg                                                                                                                                   >  2:320846/1‑250 (MQ=255)                                                                                            cAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTCCCGAGGAATACTCGATTAAAACCGCTg                                                                                                                      >  2:204787/1‑251 (MQ=255)                                                                                             aGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTgg                                                                                                                     >  2:466347/1‑251 (MQ=255)                                                                                                     gTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGt                                                                                                             >  1:393374/1‑251 (MQ=255)                                                                                                           ctTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGGGGAATACTCGATTAAAACCGCTGGCGACCAGTTCAc                                                                                                         <  2:192137/249‑1 (MQ=255)                                                                                                                 gTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGcc                                                                                                 <  1:363718/251‑1 (MQ=255)                                                                                                                    ccAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCCTGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAACCCGCTGGCGACAAGTTCACGCAATGCCTGt                                                                                              >  2:581032/1‑251 (MQ=255)                                                                                                                           gATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGa                                                                                        >  1:5303/1‑250 (MQ=255)                                                                                                                                 tgtgTCGTTGAACGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAAAGCCTGGTGGTGAATATTAc                                                                                 >  2:386793/1‑251 (MQ=255)                                                                                                                                  gtgtCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGcc                                                                                                 <  1:127112/234‑1 (MQ=255)                                                                                                                                  gtgtCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGcc                                                                                                 >  2:127112/1‑234 (MQ=255)                                                                                                                                  gtgtCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACg                                                                                <  1:544682/251‑1 (MQ=255)                                                                                                                                              ggCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATggg                                                                    >  2:117613/1‑251 (MQ=255)                                                                                                                                                              gCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGc                                                    <  2:623753/251‑1 (MQ=255)                                                                                                                                                              gCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGc                                                    <  1:437106/251‑1 (MQ=255)                                                                                                                                                                    cGTTTACAGAACAGAATGATTCACAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGCAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGCTTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGTATCTTACGTTTGAGGATGGGCAGACCATACCGGTGCGGacgc                                              >  2:268555/1‑251 (MQ=255)                                                                                                                                                                                agaaTGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAcc                                  <  1:320846/251‑1 (MQ=255)                                                                                                                                                                                      atacacaGTGCCGCAAAGCATTCATTTCTTCACAAACGGTTGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCACGCGCAGCCGCAACTGCCCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGCGGCATACTCGATTAAAACCGCTGGCGCCCAGGTCCCGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTCGACACTACAGGTGCGGACGCACGCCTCCACCCAGCat                             >  2:361130/7‑250 (MQ=255)                                                                                                                                                                                           aaGTGCTGCAAGGCATTCATTTCTTCAAAAACGGTAGCGTAACTGACCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGACTCTTAAGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCCGCCTATGGtt                       >  2:37130/1‑251 (MQ=255)                                                                                                                                                                                           aaGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGtt                       >  1:577197/1‑251 (MQ=255)                                                                                                                                                                                             gTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGc                     <  1:466347/251‑1 (MQ=255)                                                                                                                                                                                                ctgcAAAGCATTCATTTCTTCAAAAACGGTAGAGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCCGAATCCGTACCGAGGAGTACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGCTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACAAGAATATGGTTGCGGt                  >  1:262628/1‑251 (MQ=255)                                                                                                                                                                                                  gcAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGAACAGTTCACGCAATGCCTGTTGGAGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCggat                 >  2:376249/1‑248 (MQ=255)                                                                                                                                                                                                    aaaGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTaaa              <  1:541568/251‑1 (MQ=255)                                                                                                                                                                                                      aGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGTGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGTACGCCTCCAACCAGCATATGGTTGCGGTTAAACa            <  1:458606/251‑1 (MQ=255)                                                                                                                                                                                                      aGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACa            <  1:351221/251‑1 (MQ=255)                                                                                                                                                                                                      aGCATTCATTTCTTCAAAAACGGTAGCGTAAATGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCCCGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAAc             >  1:515814/1‑250 (MQ=255)                                                                                                                                                                                                             attTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGAtg     >  1:234427/1‑251 (MQ=255)                                                                                                                                                                                                                tcttcAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGAtgctg  >  2:286431/1‑251 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   GGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTG  >  NC_000913/1121547‑1122004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑