Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,120,111	G→A	A159V (GCG→GTG)	solA ←	N‑methyl‑L‑tryptophan oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,120,111	0	G	A	93.8%	129.2 / 0.2	48	A159V (GCG→GTG)	solA	N‑methyl‑L‑tryptophan oxidase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (21/24);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01

AAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTC  >  NC_000913/1119873‑1120349                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               aaaCGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATc                                                                                                                                                                                                                                     <  2:468763/250‑1 (MQ=255)            aTTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCGTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGTCGAATTGCGGTGGCCGGGCAGTTGAACATTGGCGCACAGCCCGCGTCCTTCACCAGTTGTATCCAGTGTTTAATc                                                                                                                                                                                                                         <  2:233502/251‑1 (MQ=255)               tATTCTGCACGCTATAGCGGCCATCGTCCTGATACCAGGCAAATACTTTGCGTAGGGGCTGGACATGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGAGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGc                                                                                                                                                                                                                       <  1:340621/250‑1 (MQ=255)                  tcttcACGCTATAGCGGCCATCGGCCTGATACCAGGCCAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCCGTTGGATCCAGGTTTTAATCGCCAGt                                                                                                                                                                                                                   >  2:450220/1‑251 (MQ=255)                           tataGCGGACATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTgcgc                                                                                                                                                                                                          >  2:519552/1‑251 (MQ=255)                                             gATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAg                                                                                                                                                                                          <  1:192907/249‑1 (MQ=255)                                             gATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGtt                                                                                                                                                                                        <  2:189720/251‑1 (MQ=255)                                                              tggCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGGCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCTGAATCAGTTTCAAATAAGCCGATGt                                                                                                                                                                        <  2:515625/248‑1 (MQ=255)                                                                       ggCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCGTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCgg                                                                                                                                                              <  2:61523/251‑1 (MQ=255)                                                                           ggACAGGCAGCTCCGAGAGCAGGTATTTTGCCCATGTTCCCGCGCGGACAATCGCGTGTTTCGCCTGATACTCCCCGTCAGCCGGTTCAATAGGTACTCCATCAGCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCGCAGCCCGCTTCCTTCACCAGGTTGATCCAGGTTTTAATCGGCAGTTCGGTGCGCAAAAATCCGGAAGCAGTTTCAAATATGCCGGTGTAGTTGTCCGGGAc                                                                                                                                                           <  2:443590/250‑1 (MQ=255)                                                                               aGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCAGCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTa                                                                                                                                                      <  1:72009/251‑1 (MQ=255)                                                                                 gCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTAtt                                                                                                                                                    >  2:378527/1‑251 (MQ=255)                                                                                            aGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCg                                                                                                                                         >  1:597794/1‑251 (MQ=255)                                                                                             gcagagcTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTAATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTAAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAACCAGTTTCATATAAGACGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCgg                                                                                                                                        >  2:533744/7‑251 (MQ=255)                                                                                               aGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGc                                                                                                                                      >  1:218134/1‑251 (MQ=255)                                                                                                       tACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATcc                                                                                                                              >  2:484189/1‑251 (MQ=255)                                                                                                           cATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTg                                                                                                                          <  2:156944/251‑1 (MQ=255)                                                                                                                tCCCGCGCAGACAATCGCTTTTTTCGCCTGATATTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGGTGGATCCAGGTTTTAATCGCCAGTTCTCTGCGCAAAAAACCGCAATCATTTTCAAATAAGCCGATGTAGTTGTCCGTGACGCGTATTTCCGGCCAGCTGGCCATCAGCCCTTGCGCa                                                                                                                      <  2:284224/250‑1 (MQ=255)                                                                                                                            aaTCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAg                                                                                                                                                                                                                    <  1:405745/144‑1 (MQ=255)                                                                                                                             aTCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATACAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAAc                                                                                                        >  1:274341/1‑251 (MQ=255)                                                                                                                             aTCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACACCCTCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCAACAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAAc                                                                                                        >  1:579554/1‑251 (MQ=255)                                                                                                                               cGCTTTTTTCGCCAGATACTAACCGTCAGACGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTACTCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGt                                                                                                      >  1:187422/1‑251 (MQ=255)                                                                                                                                gCTTTTTTCGCCTGATAATCACCGTCAGCCGTTTCAATAGTTAAGCCATCATCGTCATGCCGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTACTCGCCAGTTCGCTGCGCAAACAACCGGAATCAGTTTCAAATACGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGCGCTTTTCAACGtt                                                                                                     >  2:319903/1‑251 (MQ=255)                                                                                                                                    ttttCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGt                                                                                                 >  2:6639/1‑251 (MQ=255)                                                                                                                                      ttCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGTCGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTCGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGtt                                                                                                <  2:440668/250‑1 (MQ=255)                                                                                                                                      ttCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTg                                                                                               >  2:431029/1‑251 (MQ=255)                                                                                                                                          ccTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCACGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCAGCGAGCTTTTCAACGTTGAGTTGCCAt                                                                                           <  2:108456/251‑1 (MQ=255)                                                                                                                                                   cACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCgg                                                                                    <  1:484189/249‑1 (MQ=255)                                                                                                                                                                gtCAATAGTTACGCCAGCATCGTCAGGACGAATTGCGGGGCCCGGGCAGGTGAACAGTTGCGCACAGCCCGCTTCCTGCACCATTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTGTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACg                                                                      <  1:300853/249‑1 (MQ=255)                                                                                                                                                                  cAATAGTTACGCCACCAGCGTCATGACGAATTGCGGTGACCGGACAGTTGAACAGTTGCGCACAGCCCGCTGCCTTCACCAGGTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTTTCCGGGACGCGTATTGCCGGCCAGCGGGTCATAATCCCTTTCGCATCGAGCTTTGCAACGTTGAGTTGCCATTGTGCGGCGCTGTGGGCGACGTTg                                                                   <  2:306083/251‑1 (MQ=255)                                                                                                                                                                                    cGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCa                                                 <  2:9987/251‑1 (MQ=255)                                                                                                                                                                                      tCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGc                                               >  2:227354/1‑251 (MQ=255)                                                                                                                                                                                          gACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCGTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGCCGTTGGCGAGAAATGTGGAATCAGCCgg                                            <  2:219098/250‑1 (MQ=255)                                                                                                                                                                                                     gTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCGTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGTAATCAGCCGGGCCAAGGTTAAt                                <  2:62714/251‑1 (MQ=255)                                                                                                                                                                                                        aCCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGAc                             >  1:528362/1‑251 (MQ=255)                                                                                                                                                                                                            ggCAGATGAAAAGTAGCGAAAAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATACGCCGATGTAGTTGTCCGGGAAGCGTATTTCCGGCAAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACATTGAGATTACATTGTTCGGCGCTGTGGGAGACGGTGGCGCGAAATGTGGAATAAGCAGGGCCAAGGTTAATGACGcc                          >  2:602291/1‑250 (MQ=255)                                                                                                                                                                                                             gCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCag                        <  1:519552/251‑1 (MQ=255)                                                                                                                                                                                                               aGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGTACGCGTATCTCCGGCCAGCGGGCCATAATCCCTTGCGCATc                                                                                                                    <  2:306759/157‑1 (MQ=255)                                                                                                                                                                                                               aGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATc                                                                                                                    >  1:306759/1‑157 (MQ=255)                                                                                                                                                                                                                gTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCACCGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGCGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGc                     >  1:145663/1‑251 (MQ=255)                                                                                                                                                                                                                gTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGc                     <  1:529778/251‑1 (MQ=255)                                                                                                                                                                                                                 ttgcaCAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCg                    >  2:176706/5‑251 (MQ=255)                                                                                                                                                                                                                   gAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTa                  <  1:431029/251‑1 (MQ=255)                                                                                                                                                                                                                    aaCAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAACCCGGACTCAGTTTAAAATAAGCCGATGAAGATGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGAATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGACGCTGTGGGGGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCCAGGTTAATGACGCCAGAGCgaa                  >  1:626449/1‑248 (MQ=255)                                                                                                                                                                                                                      cAGTTGCGCACAGCCCGATTCATTCACCAGTTGCATCCAGGATTTCATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGGTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATCATCCCTTGCGCTTCGAGCTTTTCAACGTGGAGTTGCCATTGTTCGGCGCTGGGGGCGACTTGGGCGATCAATGTGGAATCAGCCGGGCCAGAGTTAATGACGCCAGGGCGtcca                >  2:576429/1‑247 (MQ=255)                                                                                                                                                                                                                          tGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACaaaaa            <  1:6639/250‑1 (MQ=255)                                                                                                                                                                                                                                   cccGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGAtcttt  >  1:240445/1‑250 (MQ=255)                                                                                                                                                                                                                                   cccGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGAtcttc  <  1:584462/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               AAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTC  >  NC_000913/1119873‑1120349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑