Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,112,209	C→T	D449D (GAC→GAT)	opgH →	osmoregulated periplasmic glucans biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,112,209	0	C	T	100.0%	150.1 / NA	52	D449D (GAC→GAT)	opgH	osmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/25);  total (27/25)

GAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTAT  >  NC_000913/1111963‑1112448                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gAACGCCGGGATCCTTCAGCCGTCGCCGAAAGCGCCAGGTACGGATACGCTGTATGCGCGCTGTCCGCAGTTCGCGACCCGCGTGTATGAGCCACTGTTTACGGCCGGTTTGCACTTCTGGCCACTTGGCGAGCCGCACTACTGGGGACATAACGCGATTATCCGAGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCg                                                                                                                                                                                                                                               >  2:564547/1‑251 (MQ=255) gAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGAACTTCTGGCAACTTGGCGAGTCGCAATACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCATTTGCCGGTTCAATCCTGTCACATGATTTCg                                                                                                                                                                                                                                               >  1:402562/1‑251 (MQ=255) gAACGCCGGGATCATTCAGGCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGGCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCg                                                                                                                                                                                                                                               <  2:622902/251‑1 (MQ=255)   aCGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGAGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTGTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTg                                                                                                                                                                                                                                             <  1:428749/251‑1 (MQ=255)            gcATTCAGTCGTCGCCGAAAGCGGCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAgcggcg                                                                                                                                                                                                                                    <  2:589302/250‑1 (MQ=255)              agtCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCGCTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGTTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGtt                                                                                                                                                                                                                                  <  1:281796/249‑1 (MQ=255)              aTTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGtt                                                                                                                                                                                                                                  >  1:315172/1‑251 (MQ=255)                  aGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATg                                                                                                                                                                                                                              <  1:508161/251‑1 (MQ=255)                        cgCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGCGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGt                                                                                                                                                                                                                        >  2:529177/1‑251 (MQ=255)                        cgCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGt                                                                                                                                                                                                                        <  1:27048/251‑1 (MQ=255)                         accGAAAGCGCCCGGTATGGATACGCTGTATGCGCGCTGGCAGCAGTCCGCTACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGtt                                                                                                                                                                                                                       <  2:350642/250‑2 (MQ=255)                              aaGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGt                                                                                                                                                                                                                  <  2:48551/251‑1 (MQ=255)                                  gcccGGTATGGATACGCTGTATGCTCGCTGTCAGCAGTTCGCTACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTCCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGTACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGTCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTggg                                                                                                                                                                                                              <  2:603430/249‑1 (MQ=255)                                      ggTATGGATACGCTGTATGCGCGCGGTCAGCACTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGAGTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTGAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTggggtgt                                                                                                                                                                                                          <  2:286133/251‑4 (MQ=255)                                         aTGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGGTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTg                                                                                                                                                                                                      <  2:260864/251‑1 (MQ=255)                                                  cTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCtt                                                                                                                                                                                              >  1:171593/1‑250 (MQ=255)                                                    gTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTAc                                                                                                                                                                                            <  1:3784/250‑1 (MQ=255)                                                    gTATGCGCGCTGTCAGCAGTTCCCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACAGAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTa                                                                                                                                                                                             <  1:51687/249‑1 (MQ=255)                                                            gcTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCg                                                                                                                                                                                   <  1:160566/251‑1 (MQ=255)                                                               gTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCgg                                                                                                                                                                                  <  1:239833/249‑1 (MQ=255)                                                                    cagTTCGCGACCCCCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTTAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTa                                                                                                                                                                           <  2:358515/251‑1 (MQ=255)                                                                                    tgtATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCt                                                                                                                                                           >  1:190180/1‑251 (MQ=255)                                                                                       aTGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAAc                                                                                                                                                        <  2:453948/251‑1 (MQ=255)                                                                                                     aCAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGc                                                                                                                                          <  1:263649/250‑1 (MQ=255)                                                                                                         ccGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTaaa                                                                                                                                      >  2:243605/1‑250 (MQ=255)                                                                                                         ccGGTTTGCACTTCTGGCAACTTGGCACGTCGCACTACTGGGGACATAACGCTATTATCCGCGTGAAACCGTGTATCGATCATGGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGGCGGTTCAATCCTGGCACATGATTTCGTGGAAGCGTCGTTGATGCGCCTTGCAGTTTGGGGGG‑TCTGTATTTCTTACGATCTCCCGTGTTCTGATGAAGTATTGCCTGCTAACTTGCTTGA‑TGATCTaaaa                                                                                                                                     <  2:402562/251‑1 (MQ=255)                                                                                                                gCACTTCTGGCAACTTGGCGCGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGAcc                                                                                                                              >  2:245300/1‑251 (MQ=255)                                                                                                                                          actactGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCtgatg                                                                                                    >  1:380545/1‑251 (MQ=255)                                                                                                                                           ctactGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCtgatg                                                                                                    >  1:130223/1‑250 (MQ=255)                                                                                                                                                 gggACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGAGAAATGGCTCCGCTGCCGGCCGACGGTTACTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATCCGCCGTGCAGGCTGGGGGG‑ACTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGACCTTcc                                                                                             >  1:567625/1‑251 (MQ=255)                                                                                                                                                           gcgATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCt                                                                                   >  2:545615/1‑251 (MQ=255)                                                                                                                                                             gATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTgg                                                                                 >  2:622528/1‑251 (MQ=255)                                                                                                                                                                        tGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGACCTTCCGTCTGTTCCTGGTGAAGGGTATg                                                                      >  2:213604/1‑251 (MQ=255)                                                                                                                                                                            aCCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGCAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGGGAAGGGCATGCAcc                                                                  >  1:408334/1‑251 (MQ=255)                                                                                                                                                                                  tATCGAGCACGGCGCCCGGGCTCCGCTGCGGGGAGAGGGTTCTTTTGCCGGTGCATTCCTGTCACATGATTTCGTGGAGGGGGGGTGGAGGCGCCGTGCAGGTGGGGGGG‑TCGGGATTGCTTACGATCCCCCGGGTTCTTATGAAGAATTGCCGCCTACCTTGCTTGA‑TGATCTAAAACGTGCCCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGtt                                                             <  2:190180/250‑1 (MQ=255)                                                                                                                                                                                        gccctgcccactgtgcccGCTGCGGGGGGAAGGTTCCTGTGCCGGTGCAGTCGTGTCACAGGGTTGCGGGGAATGGGCGTTGATGCGCGGTG‑AGTTGGGGGGGGTCGTGATTGCTTGTGATCCCCCGGTTTCTTATAAAAAATTCCCGCCTACCTTG‑TTGAATGAGCAAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCTCCCGGTTCACCGtt                                                      <  2:355391/235‑2 (MQ=255)                                                                                                                                                                                          aCTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCg                                                    >  2:522465/1‑251 (MQ=255)                                                                                                                                                                                                   tGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGACTTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTg                                           >  1:547452/1‑251 (MQ=255)                                                                                                                                                                                                    ggCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACCTGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTg                                           >  2:331727/1‑250 (MQ=255)                                                                                                                                                                                                      cTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCCGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACg                                        >  1:557335/1‑251 (MQ=255)                                                                                                                                                                                                      cTCCGCTGCCGGGCGAAGGTTACTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑ACTGGATTGCTTACGATCTCCCGGGTTCTTATGCAGAATTGCCGCCTACCTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACg                                        >  2:262148/1‑251 (MQ=255)                                                                                                                                                                                                               cGGGCGAAGGATCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGCTTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTACCTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCTTGCGGTGTTCCTGACGGGCGTGATg                               >  2:224141/1‑251 (MQ=255)                                                                                                                                                                                                                gggCGAAGTTTCCTTGGCGTGTTAAACCCTGTCACAGTATTCCGGGGAAGGGGCGTTGGTGCGCCGTGCAGGTGGGGGGG‑TCTGGTTTGTTTACGATCCCCCGGGTTCTTAGAAAGAATTGCCGCTTATCTTGTTTGA‑TGAGCTAAAACTTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGt                              <  1:92853/251‑1 (MQ=255)                                                                                                                                                                                                                gggCGAAGTTCCCTTTGCCGTTTCAACCCTTTCCCATGATTTCGTGGAAGCGGCTTTGATGCGCCGGGCTGTTTGGGGGT‑TCTGGATTTCTTACGATCTCCGGGTTTTTTATAAAAAATTGCCGCCTAACTTGCTGGA‑TGACCTAAACCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGt                              <  1:386382/251‑1 (MQ=255)                                                                                                                                                                                                                gggCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGt                              >  1:462773/1‑251 (MQ=255)                                                                                                                                                                                                                    gAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGtttt                           >  1:64646/1‑247 (MQ=255)                                                                                                                                                                                                                       ggTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGACGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGCATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGACACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGTCGTGATGTTTTAtct                       >  2:274664/1‑251 (MQ=255)                                                                                                                                                                                                                         ttCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTAtctct                     >  2:280077/1‑251 (MQ=255)                                                                                                                                                                                                                           ccttgtcgggttacttgtgggcccaTGATTTCGTGGAGGCGTCGTTGTTGCGCCGTGAGGGTGGGGGGG‑GCTGGATTCTTTACGATCCCCGGGGTTTTTAGAAAGAATTCCCGCCTACCTTGCTTGT‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTTTTCCTGACGGGCGTGATGTTTTAtctctc                    <  1:384203/227‑1 (MQ=255)                                                                                                                                                                                                                              ttGCCGGTTCAATCCTGTCCCATGTTTTCGTGGAGGCGGCGTTGATGCGCCGTGCGGGTTGGGGGG‑TCTGGATTGCTGACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGAAAAAACGTCACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTctccgc                 <  1:432886/250‑1 (MQ=255)                                                                                                                                                                                                                                  aggTTCAATACTGTCAACTGATTTCGTGGAAACGGCGTTGATGCGCCATGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCAGATGGTGCAACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCAGGTTCACCGTGCGGTGTTCCTGACGGGAGTGCTGTTTTATCTCtcagctacgc            >  2:519908/2‑251 (MQ=255)                                                                                                                                                                                                                                            cTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTAt  >  2:438315/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGG‑TCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGA‑TGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTAT  >  NC_000913/1111963‑1112448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑