Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,110,033	G→A	Q233Q (CAG→CAA)	opgG →	osmoregulated periplasmic glucans biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,110,033	0	G	A	100.0%	133.6 / NA	45	Q233Q (CAG→CAA)	opgG	osmoregulated periplasmic glucans biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/21);  total (24/21)

CGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGG  >  NC_000913/1109790‑1110266                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          cGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGACGAATTTCCGCGCTTCAAAGAGTTCTGGCTCGAGCGTCCAAAACCGCCTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGaaa                                                                                                                                                                                                                                     >  1:408996/1‑250 (MQ=255)  ggggCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGAGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAat                                                                                                                                                                                                                                   >  1:462628/1‑251 (MQ=255)                      gATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGAAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCg                                                                                                                                                                                                               <  2:338682/251‑1 (MQ=255)                               aggttaggttGATGGCCTTGCTGCCCGCTGCCTGGCAATTGATACCGCCTTGCCATCGGGCGAATAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTgt                                                                                                                                                                                                      <  2:5424/251‑2 (MQ=255)                                ggacaggtTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGCCTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATTTATCTGCGCGATAAAGTCGGCACACTggg                                                                                                                                                                                                     >  2:386500/4‑251 (MQ=255)                                                          cgGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTACCCAGTATGTTcc                                                                                                                                                                           <  2:181374/251‑1 (MQ=255)                                                            gCCTGGCAATTTATGCCGCCTTGCCATCGGGGGAAGAATTACCGCGCTTCAAAGAGTTCTTGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAAGTCGGGGTTATGCCATGGCGTGACACGGTTGTTGATTTGCGATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTTGGGTTCGCATCGTTTACCAGTATGTTCCt                                                                                                                                                                          <  2:97289/250‑1 (MQ=255)                                                                ggCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGGGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACTGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTg                                                                                                                                                                     <  2:504599/251‑1 (MQ=255)                                                                   aaTTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGc                                                                                                                                                                  <  1:175652/251‑1 (MQ=255)                                                                      tGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACGGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTTCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCg                                                                                                                                                                <  2:103526/250‑1 (MQ=255)                                                                        aTACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGGCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGGGCGACAGGTGCTTACAAATTCGTGGTTAGGCCAGGGCGTGACACGGGTGTGGATGTTCAATCGAAATTCGATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTTCTGTTTGGGCCGaac                                                                                                                                                             <  2:41998/251‑1 (MQ=255)                                                                                atGCCATTGGGTGAAAGATTTCCGGCCTTCCAAGAGTTCTGGATCGAGCTTCCAAAACCGACTGATAAACTTTGAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGGTGTGGATGTGCAATCGAAAATCTATCTTCGCGAGAAAGTCGGCAAACGGGGGTTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTc                                                                                                                                                     <  2:364512/250‑1 (MQ=255)                                                                                       cGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGc                                                                                                                                               <  1:283663/250‑1 (MQ=255)                                                                                                   ttCCGCGCTTAAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGCTAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGGTGTGCCATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGCACCAACCGTCGCCTGCAACTCACTATCGt                                                                                                                                  >  2:277897/1‑251 (MQ=255)                                                                                                    taagcgcTCCAACGAGTTCTGGATCGAGCGTCCAAAACCGACTGCTACACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGAGACAGGCGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGAAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAAACAGAATGCTCCTGTTTGGGACGAACCAACCGTCGCCTGCAAATAACTATCgcc                                                                                                                                 >  2:356441/4‑249 (MQ=255)                                                                                                      cgcgCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGGCGGCAAACTGGGGGTTGCACCGGTAACCAGTATGTTCCGGTTTGGGCGGAACCAACCGGCGCCTGCAAATAACTATCGTCCg                                                                                                                               <  2:408996/251‑1 (MQ=255)                                                                                                        cgcTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACGCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTAGGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGa                                                                                                                             <  2:498219/251‑1 (MQ=255)                                                                                                           ttCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGtt                                                                                                                          >  1:193838/1‑251 (MQ=255)                                                                                                              aaaGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGcc                                                                                                                       >  1:424200/1‑250 (MQ=255)                                                                                                               aaGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCAc                                                                                                                      >  2:201822/1‑251 (MQ=255)                                                                                                                 tagatCTGGATCGAGCGTCCAAAACCGATTGAAAATCGTTTAACCAGTTACTCAGTACTTTCCACCCTGCGTGAGACAGGTGATTACAAAGTCGTTGTTATGCCTGGGCGTGACACGGTTGTTGATGTGCAATCGAAAATCTATCTGTGCTATAAAGTCGTCCAACGGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCTGAACCAACCGTCGCTTGAAAATAACTATCGTCCGGAGtggctgt                                                                                                                     <  2:434179/246‑7 (MQ=255)                                                                                                                         gATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTCTGCATTGCTTGCCTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTCTGCAAGGGCGTGCCACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACg                                                                                                            >  2:611271/1‑251 (MQ=255)                                                                                                                         gATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACg                                                                                                            <  1:163308/251‑1 (MQ=255)                                                                                                                         gATCGAGCGGCCAAAACCGACTGATAAACGGTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACg                                                                                                            <  1:611271/251‑1 (MQ=255)                                                                                                                          aTCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACgg                                                                                                           <  2:152406/251‑1 (MQ=255)                                                                                                                              aGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGTCGTGACACGGTTGTGGATGTGCAATCGAAACTCTATCTGCGCGATAACGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGACCCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTACCGGtctc                                                                                                       >  1:81628/1‑251 (MQ=255)                                                                                                                                     aaaaCCGACTGATAAAAGTTTAACGATATATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATTCCAGGGCGTGACACGGTTGTGGCTGTGCAATCGAAAATCTATCTGCGCGATAACGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGATTGGGCCGAACCAACCGTCGCCTGCAAATCACTATCGTCCGGAGTTGCACGACTCTATCGGTCTCTCTATcc                                                                                                >  2:360030/1‑251 (MQ=255)                                                                                                                                             cTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTATCTATCCATGCCGGt                                                                                        >  2:398171/1‑251 (MQ=255)                                                                                                                                              tGATAAAAGTTTCACCCTCTATGCATTGCTTGACTCGCCGCGTGCGAAAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTCTCTGCGCGATAAAGACGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAACTAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCggg                                                                                        >  2:620262/1‑249 (MQ=255)                                                                                                                                                     cGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCg                                                                                 >  1:28517/1‑250 (MQ=255)                                                                                                                                                          aaCCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATgg                                                                            >  2:506869/1‑250 (MQ=255)                                                                                                                                                                 tATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATcc                                                                                                >  2:319619/1‑223 (MQ=255)                                                                                                                                                                 tATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATcc                                                                                                <  1:319619/223‑1 (MQ=255)                                                                                                                                                                 tATGCATTGCTTGAATCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCg                                                                    >  1:200758/1‑251 (MQ=255)                                                                                                                                                                     cATTGCTTTTCTCGCCTCGTGCGACATGTGCTTTCAAATTCTTGGTTATGCCAGTGCGTTACTCTCTTGTTGTTGTGCTATCTATAATCTATCTGCTCTATAAAGTCTGCAAACTGGGGTTTGCACCGTTTTCCATTATTTTCCTGTTTGTTCCTAACCATCCTTCGCCTGCAAATAACTATCTTCCGGAGTTGCACTTCTCGAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTcc                                                                 <  1:425141/250‑1 (MQ=255)                                                                                                                                                                       ttGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGtt                                                              >  1:205450/1‑251 (MQ=255)                                                                                                                                                                         gCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGCTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAACGGATCTGGCGTCCGTTgc                                                            >  2:196570/1‑250 (MQ=255)                                                                                                                                                                                 cgccgcGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCg                                                    <  1:506869/251‑1 (MQ=255)                                                                                                                                                                                  gccgcGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCg                                                    >  2:141473/1‑250 (MQ=255)                                                                                                                                                                                          cGACAGGTGCTGACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGAGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGGCCGGAATTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTa                                           <  1:360030/251‑1 (MQ=255)                                                                                                                                                                                           gACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACAttt                                            >  1:451136/1‑249 (MQ=255)                                                                                                                                                                                              aGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGCGGATGCGCAATCGAAAATCTATCTGCGCGCTAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGTAACATTTAGCgg                                       >  2:592402/1‑251 (MQ=255)                                                                                                                                                                                                           ttCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTtctc                          >  1:227108/1‑251 (MQ=255)                                                                                                                                                                                                                              gTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGc       >  1:444861/1‑251 (MQ=255)                                                                                                                                                                                                                                   acGGTTGTGGATGGGCAATCGAAAATCTATCGGCGCTATAAAGTCGGCAATCTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGGCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGAGCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTgg  <  2:355166/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          CGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGG  >  NC_000913/1109790‑1110266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑