| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,109,740 | C→T | L136F (CTT→TTT) | opgG → | osmoregulated periplasmic glucans biosynthesis protein G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,109,740 | 0 | C | T | 95.2% | 52.7 / ‑5.3 | 21 | L136F (CTT→TTT) | opgG | osmoregulated periplasmic glucans biosynthesis protein G |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (11/9); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
ATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGAC > NC_000913/1109499‑1109978 | aTACGCGGACGATCAGCAGATCCAGTTTAATCATGATAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTAGAGTTCTATCAGCAGGGTATGTACTTCGATACCCCGGGCAAAATAAATGAAGTGACTGCCACCGCAGCCAAACGAATCAAATCCAGCCCGGATGATTTCACGTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAATACCTTGGCGGTGCCGGGTTTATAGTGTTTTACCCGa < 2:101447/251‑1 (MQ=255) ttAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGCTCAACAGCAAAGATAAAAACGATGa > 2:524622/1‑250 (MQ=255) tAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCTGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGaa < 1:262775/250‑1 (MQ=255) aTCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGCTAAAAACGATGaactc > 1:65358/1‑248 (MQ=255) aaTCTGAAGACCCCATTCAAACTCGCGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCt > 1:498219/1‑250 (MQ=255) aTCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTAt > 1:181374/1‑251 (MQ=255) gAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTcc < 1:271696/251‑1 (MQ=255) aCCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGt > 2:175652/1‑251 (MQ=255) cccATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGa < 1:283268/251‑1 (MQ=255) gAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGcaggtc > 2:441476/1‑250 (MQ=255) gTACTTCGATACCCCGGTTAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGccc < 2:355025/251‑1 (MQ=255) tACCCCGGTAAGAATAAATGAAGTGAAAGCCACCGAAGTAAAACGAATCAAATACAGACAGGCTTATTTAACTTTCGGCGAAGATAAGAATGAAAAAGATAAGGAAAAAGACCTTGGCTTTGCCGGTTTTAACGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGAATGCTCGGGGCCAGCTAATTCCGCGTGATTGGTGCAGGTCAGGTT‑ATGGCCTTTCCGACCGGGGCCTggg > 2:425141/1‑250 (MQ=255) cTGCCACCGCAGTCAAACGACTCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCgggg > 1:505561/1‑250 (MQ=255) gCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTg > 1:504599/1‑250 (MQ=255) gCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCGTGCCATCGGGTGAAGAAtt < 1:212497/251‑1 (MQ=255) aGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTc > 1:355166/1‑251 (MQ=255) aTCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGGAAAAGACATTGGCTTTGCCGGTTTTAAAGTGCTTTCCCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCGGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCACCTTGCCATCGGGTGCAGAATTTCCGCACTTCa > 1:402022/1‑250 (MQ=255) aTACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGt < 1:231804/251‑1 (MQ=255) ccTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTGCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCGGGCAATTGATGCCGCCTTGCCAGCGGGTGAAGAATTTCCGCGCTTCAAAGATTTCTGGATCGAGCGGCCaaa < 2:319669/250‑1 (MQ=255) aaGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCtt < 1:413780/251‑1 (MQ=255) gTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGAc > 1:22245/1‑250 (MQ=255) | ATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGAC > NC_000913/1109499‑1109978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |