Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,090,508	C→T	E594E (GAG→GAA)	pgaA ←	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,090,508	0	C	T	100.0%	81.4 / NA	27	E594E (GAG→GAA)	pgaA	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/9);  total (18/9)

CTGTCGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCAAGATTATGTACAT  >  NC_000913/1090272‑1090752                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     cTGTCGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTACTGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCCGCGCATTCTGCCTCGAGcc                                                                                                                                                                                                                                         >  2:534438/1‑250 (MQ=255)                                    gTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGcc                                                                                                                                                                                                     >  2:442900/1‑251 (MQ=255)                                          aCGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTcgc                                                                                                                                                                                               >  1:407831/1‑251 (MQ=255)                                                 tCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAGCGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCACGCCAGTCGCGAACAAt                                                                                                                                                                                        >  2:465232/1‑251 (MQ=255)                                                   aTTTTGATACCAGCGAACATACGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCACTACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCCAGCCAGTCGCGAACAATcc                                                                                                                                                                                      >  2:94267/1‑251 (MQ=255)                                                        gaacccAGAGAAAATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTCCATGGCCCGTATTGGATCGCGGGGGGAGAGGCTTTCAAGTTGCCAACCAATCCGCCATGTATCATAAAAGTCATCCCACCCAGACAGGCGCGCTCCGGTTTT‑ACGCTCAGGATTAAAACCGGCTTCAGCTTATTCTGCCGCTAGCAAGAAATTACGCTACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCttt                                                                                                                                                                                  <  1:62277/246‑1 (MQ=255)                                                           aCCAGCGAACATAAGCCTGAGCCCTGTTGCCTGTAACACCATTTTTCATTGCCCTTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGTTTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCt                                                                                                                                                                              <  1:139850/251‑1 (MQ=255)                                                             agggaacctaaagccggggCACTTTTGCTTGTACCACCATTTTTCATTCCCAGTAAGGAAACGGGGTGAGGGATGCTTTCCCGTTTCGACCAAATCCCCCAGTTATCATAAAAACCATACCACCCAGACAGGCGCGCGCGGGTTTT‑ATGCTCATGATTAAAAACGCGTTGGGCGTATTCGGCCCCGAGCAAGATTTTACGTGTCCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTc                                                                                                                                                                            <  2:485841/233‑1 (MQ=255)                                                                     ataagcttaagaaCTGTTGCCTGTAACACAATTTTTCTTTGCCGGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCAAACCAATCCGCCAGTTATCATAAAAAACATCCCAGCCAGACGGGCGCGCGCCGGGTTT‑ATGCTCAGGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAAtt                                                                                                                                                                    <  1:487952/239‑1 (MQ=255)                                                                                  cTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCAt                                                                                                                                                        >  1:465082/1‑250 (MQ=255)                                                                                     tggCCTGTAACCCCATTTTTCATGGCCCGAAAGGGAACGCGGTGAGAGAGGCGTTCCAGTTGCAAACCAATACGCCAGTTATAATAAAAATCATACCAGCCAGACGGGCGCGCGCCGGGTTT‑ATGCCCAGGATTGAAACGGCGTTCAGCGTATTCTGCCCCGAGCCAGATATTACGGGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAAc                                                                                                                                                     <  1:248865/248‑1 (MQ=255)                                                                                        ccTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGa                                                                                                                                                  >  2:142153/1‑250 (MQ=255)                                                                                                   aTTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAAc                                                                                                                                      >  2:466290/1‑251 (MQ=255)                                                                                                                       gagcgcgGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGc                                                                                                                  >  2:615837/4‑251 (MQ=255)                                                                                                                                                                 aGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGttt                                                                         >  1:389381/1‑250 (MQ=255)                                                                                                                                                                    tATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATACCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGCAGGTGGGTAAGTCGACATCATGTTTAcc                                                                      >  1:108484/1‑250 (MQ=255)                                                                                                                                                                        aTTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGAGTTCAGCGTATTCTGACTCGAGCCAGATATTACGTGACCGCCCCTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCCGTTATCCTTCAGCGGTGGTGAATAAACGCTGGTGGTTAAGTCGAACTCATGTTTACCACTAt                                                                 >  2:289484/1‑251 (MQ=255)                                                                                                                                                                            aaaTAATACCAGCCAGACAGGCGCGCCCCGGTTTT‑ATGCTAAGTATAAAAACCCCGTCCACCTTATTCGCCCGCGGGCCGGATATTCCGTGCCCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCgg                                                             <  2:108108/251‑1 (MQ=255)                                                                                                                                                                             aaccATACCATCCAGACAGCCGCGCGC‑GGGTTTGATGCCCATGATTGAAACCGCTTTCAGCTTTTTCTCCCTCGAGCCAGATATTACGGTACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGTTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCggg                                                            <  1:289484/248‑1 (MQ=255)                                                                                                                                                                                   aCCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCggtg                                                                                                              >  1:24465/1‑195 (MQ=255)                                                                                                                                                                                            aCAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAAtt                                             >  1:485897/1‑251 (MQ=255)                                                                                                                                                                                             cAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTc                                            >  1:436710/1‑251 (MQ=255)                                                                                                                                                                                              aGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTcc                                           >  2:158571/1‑251 (MQ=255)                                                                                                                                                                                                                 ttcTCATGATTAAAAGCGCGTTCAGCGTATTCGCCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATAc                          <  1:176359/248‑1 (MQ=255)                                                                                                                                                                                                                  gCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGACCCCGATTTTACGTGACCGCCACTCACCACCAGCACGCCACTCCCGCACAATCCCTTTTCCTTCGCTCAATTGTCCATCGGCATAACCGACTCCAGCACAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGAtccg                         >  2:572227/1‑247 (MQ=255)                                                                                                                                                                                                                                aCGCTTTCAGCGTATTCTCCCCCGAGCCAGTTATTCCGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCAAGAtt          <  1:442900/251‑1 (MQ=255)                                                                                                                                                                                                                                        aGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCAAGATTATGTACAt  >  2:505449/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     CTGTCGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTT‑ATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCAAGATTATGTACAT  >  NC_000913/1090272‑1090752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑