Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,086,153	C→T	G123R (GGG→AGG)	pgaD ←	poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,086,153	0	C	T	100.0%	107.1 / NA	34	G123R (GGG→AGG)	pgaD	poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/15);  total (19/15)

CAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCC  >  NC_000913/1085907‑1086394                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  cAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTctgg                                                                                                                                                                                                                                               >  2:526815/1‑251 (MQ=255)   aTGCGATATGCCTAGCGGCGTATGCTCCGGCTTCAGTGACGCGCTGGAACGTTTCGAAGACGATGAAATGGTTGGGATCGTCCGGCTCGGTAAAGCGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACCGTTAAGTGTGTTATCGGCGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCAGCAACAACCATTTTTATTTGCCTctggct                                                                                                                                                                                                                                             >  2:378817/1‑251 (MQ=255)           tGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAg                                                                                                                                                                                                                                      <  2:287542/250‑1 (MQ=255)                  cggcgTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACCTTGCCAACGTACGATGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCt                                                                                                                                                                                                                              >  1:619264/1‑251 (MQ=255)                                                 gAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGAt                                                                                                                                                                                                 >  1:302905/1‑249 (MQ=255)                                                 gAACGTGTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATag                                                                                                                                                                                               <  2:342483/251‑1 (MQ=255)                                                                 aTGAAATGGTTGTGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCTGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGACCGGGCGAACCGGGCTTTGTTTTTGGTGGTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTGTTTTGTAGTTGCTGATAGAGCTCTTCAGGTATTg                                                                                                                                                                               <  2:291427/251‑1 (MQ=255)                                                                             ggATCGGCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCGTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTg                                                                                                                                                                   <  1:561012/251‑1 (MQ=255)                                                                                     cgggtCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCAGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTTGGTTTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCATCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCt                                                                                                                                                           <  1:355108/247‑1 (MQ=255)                                                                                     cGGTTCGGTAAAGAGGACTGCGTACGTTCGGCTCTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGGGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCt                                                                                                                                                           <  2:598242/251‑1 (MQ=255)                                                                                        ttCGGAAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCCTTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTgc                                                                                                                                                        >  1:441918/1‑251 (MQ=255)                                                                                            gTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGAGAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTgcggcg                                                                                                                                                    <  1:312245/251‑1 (MQ=255)                                                                                               aaGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGtat                                                                                                                                                 >  1:202590/1‑251 (MQ=255)                                                                                                agagGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAGGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGAATTGCTAAGCTCTCTGCATATTCTTGCGGCGtat                                                                                                                                                 >  1:207273/1‑250 (MQ=255)                                                                                                                   gCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGAtgc                                                                                                                             >  2:465366/1‑251 (MQ=255)                                                                                                                   gCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGAtgc                                                                                                                             >  2:41588/1‑251 (MQ=255)                                                                                                                          gCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGtt                                                                                                                      <  2:178662/251‑1 (MQ=255)                                                                                                                             aaCGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGttttt                                                                                                                   <  2:212635/251‑1 (MQ=255)                                                                                                                                 tACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGaa                                                                                                                >  2:182711/1‑250 (MQ=255)                                                                                                                                  aCGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGaaa                                                                                                               <  1:188579/250‑1 (MQ=255)                                                                                                                                        cATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGc                                                                                                        >  2:277973/1‑251 (MQ=255)                                                                                                                                                      aGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAgcgc                                                                                          >  1:627904/1‑251 (MQ=255)                                                                                                                                                                  gTGCAGAGCCCTGGCGAACCGGGATTTGTTTTGGGTGTTTATGCCCGGACTTGCGCTTTTTCTGAACCAACCATTTTTATTTGCCTCTGGCTGGTGCAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGATCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAACCGCGGCTTATTGTACCGCGACCAGACAATTa                                                                               >  2:541582/1‑250 (MQ=255)                                                                                                                                                                    gCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAac                                                                             >  1:392285/1‑250 (MQ=255)                                                                                                                                                                         gCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTa                                                                                                                                                                                                          >  2:230706/1‑120 (MQ=255)                                                                                                                                                                         gCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTa                                                                                                                                                                                                          <  1:230706/120‑1 (MQ=255)                                                                                                                                                                                      gggCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTg                                                          >  2:621836/1‑251 (MQ=255)                                                                                                                                                                                      gggCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTg                                                          <  2:202590/251‑1 (MQ=255)                                                                                                                                                                                                         tATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTg                                       >  1:469681/1‑251 (MQ=255)                                                                                                                                                                                                                ggACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCgg                                <  2:392285/251‑1 (MQ=255)                                                                                                                                                                                                                              tCTGAAACAACCATTGTTATTTGCTTCTGGCTGGGTAAGTGTACGCGCATCCTTTGGCTTTTTGGTAGTTGCTGATAGATCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTGATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGc                   <  1:236940/250‑1 (MQ=255)                                                                                                                                                                                                                                tGAAACAACAATTTTTATTTGTCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTGATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACACTTAACACGACGGCATTCGCCACTGCCCGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCttt                >  2:245470/1‑251 (MQ=255)                                                                                                                                                                                                                                             tttATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAAcc   >  1:384532/1‑251 (MQ=255)                                                                                                                                                                                                                                               tATTTGCCTTTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAAccc  <  2:499127/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  CAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCC  >  NC_000913/1085907‑1086394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑